kettin, D-Titin, Titin, Ket, l(3)dre8
Gene model reviewed during 5.40
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.45
Gene model reviewed during 5.55
None of the polypeptides share 100% sequence identity.
4796 (aa); 540 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\sls using the Feature Mapper tool.
Ket transcripts are first expressed at stage 11 in the mesoderm. They are expressed in most if not all muscles until the third instar larval stage. At stage 14, they are expressed in nearly all somatic, visceral, and pharyngeal muscles. Later they are expressed in muscle attachment sites.
Ket transcripts are first detected in early stage 11 embryos. Expression is observed mainly in the hindgut and posterior midgut invagination. By late stage 11, expression is observed throughout the mesoderm and can be seen in the progenitors to the pharyngeal muscles and salivary glands. By stage 12,13, the mesodermal pattern has resolved into a parasegmental pattern. By stage 13,14, expression is present in both somatic and visceral mesoderm.
sls protein can be detected in scattered aggregates dispersed in the cytoplasm of muscle cell precursors as early at 30 hrs APF. By 48 hours sls, along with other myofibrillar proteins, have organized into Z-bands.
The protein is enriched at the membrane of fusion competent cells and primarily cytoplasmic in founder cells in the developing muscle system. In myotubes sls protein islocated near the points of contact with myoblasts.
GBrowse - Visual display of RNA-Seq signalsView Dmel\sls in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Titin CG1915
Source for merge of: sls l(3)S002001
Source for merge of: Ket Titin
Source for merge of: Ket l(3)rL182 l(3)62Ca
Source for merge of: sls Ket
Source for merge of: sls CG18857
Source for merge of: sls CG18242
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
sls may be involved in the morphogenesis of leg imaginal discs, and it is necessary for condensation and separation of sister chromatids during mitosis.
sls is essential for the formation and maintenance of normal sarcomere structure of muscles and muscle tendons.
Mutations in sls result in chromosome undercondensation, chromosome breakage, loss of diploidy and premature sister chromatid separation. Mutants also have defects in myoblast fusion and muscle organisation.
sls has a role in myoblast fusion during myogenesis and later serves to organise myofilaments into the highly ordered arrays underlying skeletal muscle striation.
maps to clone: DS07136
Identification: Via an autoimmune serum from a scleroderma patient. During embryogenesis, sls RNA and protein accumulate in all the somatic and visceral muscles. Antisera made against two different domains of sls localize this portion of the protein to the Z-discs of both larval visceral and adult thoracic muscles by confocal microscopy.
sls has been cloned and its expression pattern has been analysed. Isolated from a genomic library using human autoimmune scleroderma serum that identifies a chromosomal protein in human cells and Drosophila embryos.
sls encodes a mitotic chromosomal epitope and is expressed in the somatic musculature during late embryogenesis.
Isolated from a screen of a cDNA expression library with a human CREST serum.
Identified as the gene encoding the epitope for a monoclonal antibody, MAC155, that recognises the Z-discs of Drosophila and Lethocerus. The encoded protein may act as scaffolding in the Z-disc.
Recessive lethal mutations in this complementation group were identified by failure to complement the recessive lethality of Df(3L)R-R2.
Hemizygotes for seventeen of nineteen alleles die during the first larval instar; four of these show some escape into the second instar; two alleles cause lethality in the pupal-adult stage.