l(3)07207, Crbs, far, l(3)j1B5
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Gene model reviewed during 5.47
Low-frequency RNA-Seq exon junction(s) not annotated.
7.7, 7.5 (northern blot)
None of the polypeptides share 100% sequence identity.
2139 (aa); 234 (kD)
crb protein is used as a marker for the boundary cells that delineate the dorsal and ventral domains of the embryonic large intestine.
Four Cys-poor regions of crb are distantly
related to domains present in the C-terminal part of laminin-A chains and
merosin. This homology suggests that the Cys-poor regions may be directly
involved in its role in epithelial organization.
Component of the SAC complex, a complex composed of crb, Patj and sdt (PubMed:11740560, PubMed:10102271, PubMed:11076972). May interact with the par-6 complex, which is composed of par-6, baz and aPKC, via its interaction with Patj (PubMed:12900452, PubMed:10102271, PubMed:11076972). Interacts with other proteins with Patj and sdt via its short cytoplasmic tail (PubMed:11740560). Component of the CGX complex composed of crb, galla (galla-1 or galla-2) and Xpd (PubMed:25065591). Able to interact independently (via intracellular domain) with galla-1, galla-2 and Xpd (PubMed:25065591). Interacts with apn (PubMed:30645584).
Phosphorylated in the cytoplasmic domain.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\crb using the Feature Mapper tool.
crb transcripts are abundant in the apical region of tracheal cells from mid embryonic stage 11 until stage 14 and decrease afterwards.
crb protein is expressed in the apical membrane domain of the epithelial cells of imaginal discs and follicle cells in a reticular pattern outlining the borders of the cells.
crb protein is observed on the apical membranes of epithelial cells.
crb protein is used as a marker for the boundary cells that delineate the dorsal and ventral domains of the embryonic large intestine.
crb protein is detected in the scolopale cell lumen of the chordotonal organs.
crb protein localizes to the stalk of the rhabdomere. It is seen in this location from about 50% through pupal development only in rhabdomeres R2, R4, R5 and R7, because R1, R3 and R6 lack stalks at this stage.
Protein is observed concentrated in cells undergoing apical constriction during invagination of the salivary gland primordia.
In third instar larvae, crb protein is observed in epithelial cells but not adepithelial cells of all imaginal discs. It is also seen in the inner and outer optic lobe anlage, the imaginal ring of the salivary glands, faintly in trachea, and on the surface of garland cells. In adult males, crb protein is observed in the testicular duct, seminal vesicle, male accessory gland, ejaculatory bulb, and ejaculatory duct. In females, it is observed in follicle cells in oogenesis stages S1-S10.
crb protein is first detected during gastrulation in the ectoderm and in the endoderm but not in the mesoderm. It localizes to the apical surfaces of epithelial cells and is preferentially concentrated at the borders between neighboring cells. Between stages 8 and 11 crb protein expression is lost in the posterior midgut, in the cells at the bottom of the stomodeal invagination, and in neuroblasts before they delaminate from the ectoderm. By stage 11, crb protein is restricted to epithelial cells of ectodermal origin and is observed in the foregut, the tracheal system, the salivary glands, the Malpighian tubules, the optic lobes, and the stomatogastric nervous system. Later in embryogenesis, expression is observed in the hindgut epithelium and in external sensory organs and chordotonal organs of the PNS.
Comment: apical epithelial surface and lumen
GBrowse - Visual display of RNA-Seq signals
View Dmel\crb in GBrowse 2Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: crb CG6383
Source for merge of: crb l(3)S050920
Source for merge of: crb l(3)j1B5
Source for merge of: crb l(3)S058104
Source for merge of: crb l(3)07207
Source for merge of: crb far
Four more alleles discarded by Tubingen.
crb is required for cell polarity in the tubles only from the time when morphogenetic movements start.
The crb 3' UTR is necessary and sufficient for it's apical localization in embryos.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Overexpression of the JM region of the crb gene product recruits adherens junctions to ectopic sites of the photoreceptor cell membrane without causing loss of apicobasal polarity.
crb is required to inhibit light-induced photoreceptor degeneration.
crb is required for the formation and maintenance of the follicular epithelium.
Mutants exhibit cellular differentiation defects.
Loss of cell polarity in the epidermal primordium of crb mutant embryos is associated with a failure to establish the zonulae adherentes. These junctions fail to develop when an altered polarity is induced by the overexpression of crb. crb and sdt have different functions during the formation of the zonula adherens.
The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. crb is required for germ cell viability or early oogenesis.
crb is part of the apical membrane and is concentrated in the immediate vicinity of the zonula adherens.
crb and sdt encode critical components of a pathway that acts at the apical pole of epithelial cells to control their cytoarchitecture. Mosaic experiments suggest that sdt though not crb is required cell autonomously. Double mutant analysis suggests that sdt acts downstream of and is activated by crb.
Four Cys poor regions of crb are distantly related to domains present in the C terminal part of laminin A chains.
The homology with the C terminal part of laminin A chains suggests that the Cys poor regions of crb may be directly involved in interactions organizing epithelia.
The crb gene encodes an integral membrane protein with 30 EGF-like repeats in the extracellular domain.
crb may function to establish and/or maintain epithelia cell polarity.
crb mutants display many small holes in the cuticle.
