FlyBase Gene Report: Dmel\Pdfr

General Information

Symbol

Dmel\Pdfr

Species

D. melanogaster

Name

Pigment-dispersing factor receptor

Annotation Symbol

CG13758

Feature Type

protein_coding_gene

FlyBase ID

FBgn0260753

Gene Model Status

Current

Stock Availability

65 publicly available

Gene Summary

Pigment-dispersing factor receptor (Pdfr) encodes a G-Protein Coupled Receptor in the Family B class of GPCRs (Secretin Receptor-like) that can be activated by the product of Pdf. It has been implicated in regulation of circadian physiology, development of the flight motor system, and regulation of mating. [Date last reviewed: 2019-02-28] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

han, PDF receptor, EG:BACR25B3.3 , PDF-receptor, groom-of-pdf

Key Links

Genomic Location

Cytogenetic map

3A4-3A6

Sequence location

X:2,552,206..2,578,640 [+]

Recombination map

1-1

RefSeq locus

NC_004354 REGION:2552206..2578640

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (25 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables neuropeptide receptor activity

inferred from physical interaction with UniProtKB:O96690

(Hyun et al., 2005)

enables protein binding

inferred from physical interaction with UniProtKB:O96690

(Hyun et al., 2005)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables G protein-coupled peptide receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN001737881

(GO Reference Genome Project, 2011-)

enables G protein-coupled receptor activity

inferred from electronic annotation with InterPro:IPR000832, InterPro:IPR001879, InterPro:IPR017983, InterPro:IPR036445

(InterPro Project Members, 2004-)

enables neuropeptide receptor activity

inferred from sequence model

(Hewes and Taghert, 2001)

enables transmembrane signaling receptor activity

inferred from electronic annotation with InterPro:IPR017981

(InterPro Project Members, 2004-)

Biological Process (16 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway

inferred from direct assay

(Hyun et al., 2005)

involved_in circadian behavior

inferred from mutant phenotype

(Hyun et al., 2005)

inferred from mutant phenotype

(Lear et al., 2005)

inferred from direct assay

(Zhang et al., 2010, Mertens et al., 2005)

involved_in circadian rhythm

inferred from mutant phenotype

(Lear et al., 2005)

inferred from direct assay

(Mertens et al., 2005)

involved_in circadian sleep/wake cycle

inferred from mutant phenotype

(Parisky et al., 2008)

involved_in circadian temperature homeostasis

inferred from mutant phenotype

(Head et al., 2015)

involved_in copper ion homeostasis

inferred from mutant phenotype

(Norgate et al., 2007)

involved_in gravitaxis

inferred from direct assay

(Mertens et al., 2005)

involved_in locomotor rhythm

inferred from mutant phenotype

(Lear et al., 2009)

involved_in neuropeptide signaling pathway

inferred from direct assay

(Hyun et al., 2005)

involved_in positive regulation of calcium-mediated signaling

inferred from genetic interaction with FLYBASE:Itpr; FB:FBgn0010051,FLYBASE:Orai; FB:FBgn0041585,FLYBASE:Stim; FB:FBgn0045073

(Agrawal et al., 2013)

involved_in regulation of circadian rhythm

inferred from mutant phenotype

(Bai et al., 2018)

inferred from genetic interaction with FLYBASE:Nf1; FB:FBgn0015269

(Bai et al., 2018)

involved_in regulation of circadian sleep/wake cycle

inferred from mutant phenotype

(Chung et al., 2009)

involved_in response to paraquat

inferred from mutant phenotype

(Hajji et al., 2019)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in adenylate cyclase-modulating G protein-coupled receptor signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN001737881

(GO Reference Genome Project, 2011-)

involved_in cell surface receptor signaling pathway

inferred from electronic annotation with InterPro:IPR017981

(InterPro Project Members, 2004-)

involved_in G protein-coupled receptor signaling pathway

inferred from sequence model

(Hewes and Taghert, 2001)

inferred from electronic annotation with InterPro:IPR000832, InterPro:IPR017983

(InterPro Project Members, 2004-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in neuron projection

inferred from direct assay

(de la Paz Fernandez et al., 2007)

located_in neuronal cell body

inferred from direct assay

(de la Paz Fernandez et al., 2007)

located_in plasma membrane

inferred from direct assay

(Hyun et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR000832, InterPro:IPR001879, InterPro:IPR017981, InterPro:IPR036445

(InterPro Project Members, 2004-)

inferred from sequence model

(Hewes and Taghert, 2001)

non-traceable author statement

(Hyun et al., 2005)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001737881

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CLASS B GPCRs, SUBFAMILY B1

Protein Family (UniProt)

Belongs to the G-protein coupled receptor 2 family. (Q9W4Y2)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

CLASS B GPCRs, SUBFAMILY B1 -: The B1 subfamily of Class B GPCRs are largely classical hormone receptors. (Adapted from FBrf0147071 and FBrf0221117).

Protein Function (UniProtKB)

Receptor for PDF, a neuropeptide controlling circadian behavioral rhythms. Probably regulates circadian behavioral rhythms through coordination of activities of clock neurons. PDF-binding results in the elevation of cAMP synthesis. Plays a role in sleep regulation and regulates the state transition from sleep to wake (PubMed:19038223, PubMed:19230663).

(UniProt, Q9W4Y2)

Summary (Interactive Fly)

dopaminergic neurons that respond to PDFR signalling are sleep-promoting and during the day when PDF levels are high, they are inhibited, thereby promoting wakefulness - regulates free-running rhythmicity in Drosophila circadian locomotor activity - modulators of molecular cycling in the peripheral clocks of both the glial cells and the photoreceptors of the compound eye, regulation of night-onset temperature preference

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pdfr for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W4Y2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.39

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pdfr-RA

FBtr0070436

NM_130651

2910

669

Pdfr-RB

FBtr0308565

NM_001258572

3230

738

Pdfr-RC

FBtr0340084

NM_001297897

3331

724

Pdfr-RD

FBtr0340085

NM_001297898

3239

724

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Pdfr-PA

FBpp0099841

74.5

669

9.05

FBpp0300789

82.0

738

8.91

FBpp0309083

80.1

724

9.71

FBpp0309084

80.1

724

9.71

X2JDL2

NP_001284827

AHN59298

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

724 aa isoforms: Pdfr-PC, Pdfr-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9W4Y2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pdfr using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.77

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult protocerebrum | dorso-lateral

(Lear et al., 2005)

adult pars intercerebralis

(Lear et al., 2005)

Additional Descriptive Data

Pdfr transcript is detected in the dorsolateral protocerebrum, in a region near or including the DN1 clock neurons, and in the pars intercerebralis. The cells in the dorsolateral protocerebrum are found near the dorsal projections of the s-LNv neurons.

(Lear et al., 2005)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

interneuron | faint | subset of posterior lateral protocerebrum

(Chen et al., 2016)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

(Mertens et al., 2005)

adult protocerebrum | dorso-lateral

(Mertens et al., 2005)

adult gnathal ganglion | anterior | medial

(Mertens et al., 2005)

DN1 neuron

(Mertens et al., 2005)

DN3 neuron

(Mertens et al., 2005)

crop

Comment: >

(Veenstra et al., 2008)

Additional Descriptive Data

Pdfr is expressed in two to three interneurons with somata in the posterior lateral protocerebrum that express ScerGAL4^AstA.2.74 and AstA.

(Chen et al., 2016)

Strong expression of Pdfr protein is observed in one large cell body in the dorso-lateral protocerebrum, in 20 cells in the subesophageal ganglion, in one pair of DN1, two to three DN3 neurons and in the neuronal processes throughout the brain. Weak expression is seen throughout the brain, especially in the medulla. All neuronal processes stained by Pdf protein are also stained by Pdfr protein. Some neuronal processes in the medulla and lobula only express Pdfr.

(Mertens et al., 2005)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in neuron projection

inferred from direct assay

(de la Paz Fernandez et al., 2007)

located_in neuronal cell body

inferred from direct assay

(de la Paz Fernandez et al., 2007)

located_in plasma membrane

inferred from direct assay

(Hyun et al., 2005)

Expression Deduced from Reporters

Reporter: P{GawB}Pdfr^EY11851-GAL4

Stage

Tissue/Position (including subcellular localization)

Reference

Reporter: P{GMR18H11-GAL4}

Stage

Tissue/Position (including subcellular localization)

Reference

Reporter: P{GMR18H11-lexA}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

DN1 neuron | subset

(Sun et al., 2022, Guo et al., 2016)

DN1p neuron

(Jin et al., 2021)

Reporter: P{Pdfr-GAL4.10}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult optic lobe | restricted

(Parisky et al., 2008)

l-LNv neuron | subset

(Parisky et al., 2008)

adult s-LNv neuron | subset

(Parisky et al., 2008)

LNd neuron | subset

(Parisky et al., 2008)

ellipsoid body

(Parisky et al., 2008)

Reporter: P{Pdfr-GAL4.B}

Stage

Tissue/Position (including subcellular localization)

Reference

Reporter: P{Pdfr-myc}

Stage

Tissue/Position (including subcellular localization)

Reference

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pdfr in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pdfr

View exonic expression by tissue for Dmel\Pdfr

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31234/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0260753

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040378

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0260753

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13758

Flygut - An atlas of the Drosophila adult midgut

FBgn0260753

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 24 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 28 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pdfr

Transgenic constructs containing regulatory region of Pdfr

Aberrations (Deficiencies and Duplications) ( 8 )

Inferred from experimentation ( 8 )

Gene not duplicated in

Dp(1;1)weeb¹

(Kemppainen et al., 2009)

Gene not disrupted in

Df(1)RK2

(Lear et al., 2005)

Df(1)RK3

(Lear et al., 2005)

Df(1)RK4

(Lear et al., 2005)

Gene disrupted in

Df(1)ED411

(Lear et al., 2005)

Df(1)Sp2F-3A

(Lear et al., 2005)

In(1)sc⁷

(Lear et al., 2005)

In(1)sc²⁹

(Lear et al., 2005)

Inferred from location ( 12 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die | nutrition conditional, with Scer\GAL4^Tub.PU

Pdfr^KK110677

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pdfr^GD712

viable, with Scer\GAL4^Mef2.PR

Pdfr^GD712

viable, with Scer\GAL4^pnr-MD237

Pdfr^GD712

viable, with Scer\GAL4^tin.CΔ4

Pdfr^GD712

Other Phenotypes

Allele

abnormal circadian behavior

Pdfr⁵³⁰⁴

abnormal circadian behavior (with Pdfr⁵³⁰⁴)

Pdfr^EY11851-GAL4

abnormal circadian behavior (with Pdfr^EY11851-GAL4)

Pdfr⁵³⁰⁴

abnormal circadian behavior | adult stage

Pdfr⁵³⁰⁴

abnormal circadian behavior | adult stage, with Scer\GAL4^P2.4.Pdf

Pdfr^GD712

abnormal circadian behavior | recessive

Pdfr⁵³⁰⁴

abnormal circadian rhythm

abnormal circadian rhythm | adult stage

abnormal circadian rhythm | adult stage, with Scer\GAL4^sLNvs

Pdfr^HMS01815

abnormal locomotor behavior | adult stage

Pdfr^EY11851

abnormal locomotor behavior | adult stage, with Scer\GAL4^Cg.PU

Pdfr^KK110677

abnormal locomotor rhythm

abnormal mating behavior, with Scer\GAL4^5-HT1B-Gal4

Pdfr^HMS01815

abnormal mating behavior, with Scer\GAL4^Hand.PU

Pdfr^HMJ02073

abnormal mating behavior, with Scer\GAL4^Mhc.PK

Pdfr^HMJ02073

abnormal neuroanatomy | adult stage

Pdfr⁵³⁰⁴

abnormal neuroanatomy | adult stage, with Scer\GAL4^Pdfr.10

Pdfr^HMS01815

abnormal neuroanatomy | adult stage, with Scer\GAL4^sLNvs

Pdfr^HMS01815

abnormal starvation stress response | adult stage, with Scer\GAL4^Cg.PU

Pdfr^KK110677

abnormal starvation stress response | adult stage | nutrition conditional, with Scer\GAL4^Mef2.PU

Pdfr^KK110677

abnormal temperature response

abnormal temperature response, with Scer\GAL4^Clk.1.5

Pdfr^RNAi.UAS.cUa

abnormal temperature response | adult stage

Pdfr⁵³⁰⁴

decreased body weight | adult stage | nutrition conditional, with Scer\GAL4^Cg.PU

Pdfr^KK110677

decreased rate of movement | adult stage, with Scer\GAL4^elav.PU

Pdfr^HMS01815

long lived | calorie restriction conditional, with Scer\GAL4^Cg.PU

Pdfr^KK110677

majority die during larval stage | nutrition conditional, with Scer\GAL4^Tub.PU

Pdfr^KK110677

short lived | :calorie restriction conditional | nutrition conditional, with Scer\GAL4^Mef2.PU

Pdfr^KK110677

Phenotype manifest in

Allele

adult s-LNv neuron | adult stage

Pdfr⁵³⁰⁴

adult s-LNv neuron | adult stage, with Scer\GAL4^sLNvs

Pdfr^HMS01815

asymmetrical body | absent | adult stage, with Scer\GAL4^Pdfr.10

Pdfr^HMS01815

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (47)

Hsap\ADCYAP1R1

4 of 14

Yes

Hsap\GCGR

4 of 14

Yes

Hsap\GHRHR

4 of 14

Yes

Hsap\GIPR

4 of 14

Yes

Hsap\GLP1R

4 of 14

Yes

Hsap\GLP2R

4 of 14

Yes

Hsap\PTH2R

4 of 14

Yes

Hsap\SCTR

4 of 14

Yes

Hsap\VIPR1

4 of 14

Yes

Hsap\VIPR2

4 of 14

Yes

Hsap\CALCRL

3 of 14

Hsap\PTH1R

3 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\FZD9

1 of 14

Hsap\INMT

1 of 14

Yes

Hsap\LINC02210-CRHR1

1 of 14

Hsap\NNMT

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (20)

Rnor\Adcyap1r1

4 of 14

Yes

Rnor\Gcgr

4 of 14

Yes

Rnor\Gipr

4 of 14

Yes

Rnor\Glp1r

4 of 14

Yes

Rnor\Glp2r

4 of 14

Yes

Rnor\Pth1r

4 of 14

Yes

Rnor\Pth2r

4 of 14

Yes

Rnor\Sctr

4 of 14

Yes

Rnor\Vipr2

4 of 14

Yes

Rnor\Calcr

3 of 14

Rnor\Vipr1

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Rnor\Adgrg2

1 of 14

Rnor\Adgrg6

1 of 14

Rnor\Fbxo46

1 of 14

Yes

Rnor\Inmt

1 of 14

Yes

Rnor\Nnmt

1 of 14

Yes

Mus musculus (laboratory mouse) (22)

Mmus\Adcyap1r1

4 of 14

Yes

Mmus\Gcgr

4 of 14

Yes

Mmus\Ghrhr

4 of 14

Yes

Mmus\Gipr

4 of 14

Yes

Mmus\Glp1r

4 of 14

Yes

Mmus\Glp2r

4 of 14

Yes

Mmus\Pth1r

4 of 14

Yes

Mmus\Pth2r

4 of 14

Yes

Mmus\Sctr

4 of 14

Yes

Mmus\Vipr1

4 of 14

Yes

Mmus\Vipr2

4 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Inmt

1 of 14

Yes

Mmus\Nnmt

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (23)

Xtro\crhr1.1

3 of 13

Yes

Xtro\LOC100487239

3 of 13

Yes

Xtro\calcrl

2 of 13

Xtro\calcr

2 of 13

Xtro\gcgr

2 of 13

Yes

Xtro\pth1r

2 of 13

Yes

Xtro\sctr

2 of 13

Yes

Xtro\vipr1

2 of 13

Yes

Xtro\adcyap1r1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\glp1r

1 of 13

Yes

Xtro\glp2r

1 of 13

Yes

Xtro\lgmn

1 of 13

Xtro\LOC100492996

1 of 13

Yes

Xtro\LOC101735190

1 of 13

Yes

Xtro\pth2r

1 of 13

Yes

Xtro\s100pbp

1 of 13

Yes

Xtro\tcf3

1 of 13

Xtro\vipr2

1 of 13

Yes

Xtro\zc4h2

1 of 13

Danio rerio (Zebrafish) (30)

Drer\adcyap1r1b

4 of 14

Yes

Drer\gcgrb

4 of 14

Yes

Drer\gipr

4 of 14

Yes

Drer\pth1r

4 of 14

Yes

Drer\pth2ra

4 of 14

Yes

Drer\vipr1a

4 of 14

Yes

Drer\vipr1b

4 of 14

Yes

Drer\vipr2

4 of 14

Yes

Drer\adcyap1r1a

3 of 14

Yes

Drer\calcr

3 of 14

Drer\gcgra

3 of 14

Yes

Drer\ghrhr2

3 of 14

Yes

Drer\ghrhra

3 of 14

Yes

Drer\ghrhrb

3 of 14

Yes

Drer\pth2rb

3 of 14

Yes

Drer\pth3r

3 of 14

Yes

Drer\sctr

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\glp2r

1 of 14

Yes

Drer\zgc:64002

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\pdfr-1

11 of 14

Yes

Cele\seb-3

5 of 14

Cele\seb-2

2 of 14

Cele\anmt-2

1 of 14

Yes

Cele\lat-1

1 of 14

Cele\lat-2

1 of 14

Anopheles gambiae (African malaria mosquito) (7)

Agam\GPRCAL3

12 of 12

Yes

3 of 12

3 of 12

3 of 12

2 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pdfr. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (20)

Dh44-R1

5 of 13

Dh44-R2

5 of 13

Dh31-R

4 of 13

hec

4 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

Pdfr³³⁶⁹

model of tauopathy

CEA

(Bai et al., 2018)

Pdfr⁵³⁰⁴

model of tauopathy

CEA

(Bai et al., 2018)

Potential Models Based on Orthology ( 6 )

Human Ortholog

Disease

Evidence

References

GHRHR; growth hormone releasing hormone receptor

model of isolated growth hormone deficiency type IB

IEA

(FlyBase, 2019-)

GCGR; glucagon receptor

model of Mahvash Disease

IEA

(FlyBase, 2019-)

PTH1R; parathyroid hormone 1 receptor

model of chondrodysplasia Blomstrand type

IEA

(FlyBase, 2019-)

model of Jansen's metaphyseal chondrodysplasia

IEA

(FlyBase, 2019-)

model of primary failure of tooth eruption

IEA

(FlyBase, 2019-)

model of Eiken syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ADCYAP1R1; ADCYAP receptor type I

4 of 14

102981

GCGR; glucagon receptor

4 of 14

138033

MAHVASH DISEASE; MVAH

Mahvash Disease

GHRHR; growth hormone releasing hormone receptor

4 of 14

139191

ISOLATED GROWTH HORMONE DEFICIENCY, TYPE IV; IGHD4

isolated growth hormone deficiency type IB

GIPR; gastric inhibitory polypeptide receptor

4 of 14

137241

GLP1R; glucagon like peptide 1 receptor

4 of 14

138032

GLP2R; glucagon like peptide 2 receptor

4 of 14

603659

PTH2R; parathyroid hormone 2 receptor

4 of 14

601469

SCTR; secretin receptor

4 of 14

182098

VIPR1; vasoactive intestinal peptide receptor 1

4 of 14

192321

VIPR2; vasoactive intestinal peptide receptor 2

4 of 14

601970

CALCRL; calcitonin receptor like receptor

3 of 14

114190

LYMPHATIC MALFORMATION 8; LMPHM8

PTH1R; parathyroid hormone 1 receptor

3 of 14

168468

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Pdfr

suppressible

dco

(Zheng et al., 2014)

Pdfr

suppressible

Nf1

(Bai et al., 2018)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Ac3

suppressible

Pdfr

(Duvall and Taghert, 2012)

CkIIα

suppressible

Pdfr

(Lear et al., 2005)

CkIIβ

suppressible

Pdfr

(Lear et al., 2005)

wake

suppressible

Pdfr

(Liu et al., 2014)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0260753

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-1

Cytogenetic map

3A4-3A6

Sequence location

X:2,552,206..2,578,640 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

3A4-3A6

Limits computationally determined from genome sequence between P{EP}CG32796^EP1385&P{EP}EP1160 and P{EP}egh^EP804

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-1

(FlyBase, 2016)

1-1.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (65)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

33068

Pdfr⁵³⁰⁴ w^*

33069

Pdfr³³⁶⁹ w^*

82768

w^*; P{Pdfr-myc}attP2

68215

w^*; P{Pdfr-GAL4.B}2/CyO

Korea

13321

P{VT055854-GAL4}attP2

10774

13758R-1

13758R-3

v106381

v200573

P{VT055852-GAL4}attP2

v207324

P{VT055855-GAL4}attP2

v42724

w¹¹¹⁸; P{GD712}v42724

More stocks available...

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (13)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH51443

Other clones

MIP16931

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0260753

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0260753

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Mertens et al., 2005)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

pdfr plays an important role in regulating the per oscillation and the core clock.

(Lear et al., 2005)

pdfr does not affect Pdf expression or metabolism, but acts downstream.

(Lear et al., 2005)

pdfr mutants display increased circadian arrhythmicity and altered geotaxis that is epistatic to that of Pdf mutants.

(Mertens et al., 2005)

Functional expression of Pdf-R1in HEK 293 cells revealed clear specificity for the Pdf protein when assayed by CRE-luciferase with an EC⁵⁰ of 28 nM.

(Shafer et al., 2005)

Relationship to Other Genes

Source for database merge of

Source for merge of: pdfr Pdf-R1

(FlyBase, 1992-)

Source for merge of: EG:BACR25B3.3 CG13758

(FlyBase, 1996-)

Source for merge of: CG13758 anon-WO0170980.157 anon-WO0170980.158

(FlyBase, 1996-)

Additional comments

" EG:BACR25B3.3 " may correspond to "Scim4". Sequence analysis off ends of P{SUPor-P} in Scim insertion mutant places "Scim4" near/in " EG:BACR25B3.2 " and/or " EG:BACR25B3.3 ".

(Dobie and Karpen, 2001.4.18)

Source for merge of EG:BACR25B3.3 CG13758 was sequence comparison ( date:000425 ).

(FlyBase, 1996-)

Source for merge of CG13758 anon-WO0170980.157 anon-WO0170980.158 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Pdfr pdfr

(FlyBase, 2009-)

Source for identity of: Pdfr CG13758

(Mertens et al., 2005)

Nomenclature comments

Changed symbol from 'pdfr' to 'Pdfr' so it follows our standard nomenclature rules and to make it consistent with the capitalization of the symbol of the ligand ('Pdf'). Also changed the fullname to spell out the 'PDF' abbreviation used previously.

(FlyBase, 2009-)

Etymology

'han' refers to the Korean letter for cold.

(Hyun et al., 2005)

Synonyms and Secondary IDs (32)

Reported As

Symbol Synonym

BACR25B3.3

(Hewes and Taghert, 2001.7.31)

DmeCG13758

(Cardoso et al., 2006)

EG:BACR25B3.3

(Metpally and Sowdhamini, 2005, Radford et al., 2002, Benos et al., 2001)

Han

(Li et al., 2018, Holmes and Sheeba, 2005)

PDF-R

(Taghert, 2022)

PDF-R1

(Shafer et al., 2005)

PDFR-1

(Anderson and Adolphs, 2014)

Pdf R

(Qi et al., 2021)

Pdf-R1

PdfR

(Kozlov and Nagoshi, 2019, Liu and Jin, 2017, Talsma et al., 2012, Yao et al., 2012, Isaac et al., 2007)

Pdfr

(Agard et al., 2024, Everman and Macdonald, 2024, Peng et al., 2024, Corthals et al., 2023, Han et al., 2023, Khatib et al., 2023, Lee and Lim, 2023, Roach et al., 2023, Havula et al., 2022, Klose and Shaw, 2021, McLaughlin et al., 2021, Song et al., 2021, Flyer-Adams et al., 2020, Kondo et al., 2020, Mazzotta et al., 2020, Pegoraro et al., 2020, Brunet Avalos et al., 2019, Goda and Hamada, 2019, Goda et al., 2019, Hajji et al., 2019, Rao and Deng, 2019.10.23, Chatterjee et al., 2018, Gene Disruption Project members, 2018-, Lamba et al., 2018, Abruzzi et al., 2017, Chen et al., 2016, Crocker et al., 2016, Goda et al., 2016, Shen et al., 2016, Liu et al., 2014, Menuz et al., 2014, Yao and Shafer, 2014, Zheng et al., 2014, Krupp et al., 2013, Potdar and Sheeba, 2013, Zhang and Emery, 2013, Caers et al., 2012, Luo and Sehgal, 2012, van der Harst et al., 2012, Zhang et al., 2010, Zhang et al., 2010, Zhang et al., 2009)

anon-WO0170980.157

anon-WO0170980.158

gop

(Lear et al., 2005)

han

(Iyer et al., 2025, Johnstone et al., 2022, Song et al., 2021, Hegazi et al., 2019, Schlichting et al., 2019, Lamba et al., 2018, Chen et al., 2016, Beckwith et al., 2011, Cusumano et al., 2009, Lear et al., 2009, Picot et al., 2009, Wülbeck et al., 2009, Shafer et al., 2008, Hyun et al., 2005)

pdfR

(Górska-Andrzejak et al., 2018, Mezan et al., 2016)

Name Synonyms

CG13758

(Lear et al., 2009)

Groom of PDF

(Hegazi et al., 2019)

PDF Receptor

(Choi et al., 2009)

PDF receptor

(Vecsey et al., 2014, Bendena et al., 2012, Luo and Sehgal, 2012, Lear et al., 2009, Zhang et al., 2009)

PDF-receptor

(Veenstra et al., 2008, Isaac et al., 2007)

Pdf recep- tor

(Wülbeck et al., 2009)

Pdf receptor

(Means et al., 2015)

Pigment dispersing factor receptor

(Caers et al., 2012)

Pigment dispersing factor receptor 1

(Shafer et al., 2005)

Pigment-Dispersing Factor Receptor

(Head et al., 2015)

Pigment-dispersing factor receptor

(Yu et al., 2016)

groom-of-PDF

(Lear et al., 2005)

groom-of-pdf

(Lear et al., 2009, Lear et al., 2005)

pigment-dispersing factor receptor

(Head et al., 2015)

Secondary FlyBase IDs

FBgn0040378
FBgn0029622
FBgn0046581
FBgn0046582
FBgn0082641

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 59 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31234

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W4Y2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9W4Y2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W4Y2

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31234/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0260753

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0260753

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG13758

MARRVEL_MODEL - MARRVEL (model organism gene)

31234

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0260753

DroID - A comprehensive database of gene and protein interactions.

FBgn0260753

DRSC - Results frm RNAi screens

FBgn0260753

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040378

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0260753

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13758

Flygut - An atlas of the Drosophila adult midgut

FBgn0260753

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013682

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

References (214)

The following external sites may use different assemblies or annotations than FlyBase.

-0.77

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