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General Information
Symbol
Dmel\Pdfr
Species
D. melanogaster
Name
Pigment-dispersing factor receptor
Annotation Symbol
CG13758
Feature Type
FlyBase ID
FBgn0260753
Gene Model Status
Stock Availability
Gene Summary
Pigment-dispersing factor receptor (Pdfr) encodes a G-Protein Coupled Receptor in the Family B class of GPCRs (Secretin Receptor-like) that can be activated by the product of Pdf. It has been implicated in regulation of circadian physiology, development of the flight motor system, and regulation of mating. [Date last reviewed: 2019-02-28] (FlyBase Gene Snapshot)
Also Known As

han, PDF receptor, EG:BACR25B3.3 , groom-of-pdf, PDF-receptor

Key Links
Genomic Location
Cytogenetic map
Sequence location
X:2,552,206..2,578,640 [+]
Recombination map
1-1
RefSeq locus
NC_004354 REGION:2552206..2578640
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (26 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with UniProtKB:O96690
(assigned by UniProt )
inferred from physical interaction with UniProtKB:O96690
(assigned by UniProt )
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity
inferred from biological aspect of ancestor with PANTHER:PTN001737881
(assigned by GO_Central )
inferred from sequence model
inferred from biological aspect of ancestor with PANTHER:PTN000244369
(assigned by GO_Central )
Biological Process (16 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
involved_in circadian behavior
inferred from mutant phenotype
(assigned by UniProt )
inferred from direct assay
inferred from mutant phenotype
involved_in circadian rhythm
inferred from mutant phenotype
(assigned by UniProt )
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
involved_in gravitaxis
inferred from direct assay
involved_in locomotor rhythm
inferred from mutant phenotype
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Nf1; FB:FBgn0015269
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001737881
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR017981
(assigned by InterPro )
inferred from sequence model
inferred from sequence or structural similarity
Cellular Component (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from sequence model
inferred from sequence or structural similarity
non-traceable author statement
(assigned by UniProt )
is_active_in plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN001737881
(assigned by GO_Central )
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the G-protein coupled receptor 2 family. (Q9W4Y2)
Summaries
Gene Snapshot
Pigment-dispersing factor receptor (Pdfr) encodes a G-Protein Coupled Receptor in the Family B class of GPCRs (Secretin Receptor-like) that can be activated by the product of Pdf. It has been implicated in regulation of circadian physiology, development of the flight motor system, and regulation of mating. [Date last reviewed: 2019-02-28]
Gene Group (FlyBase)
CLASS B GPCRs, SUBFAMILY B1 -
The B1 subfamily of Class B GPCRs are largely classical hormone receptors. (Adapted from FBrf0147071 and FBrf0221117).
Protein Function (UniProtKB)
Receptor for PDF, a neuropeptide controlling circadian behavioral rhythms. Probably regulates circadian behavioral rhythms through coordination of activities of clock neurons. PDF-binding results in the elevation of cAMP synthesis.
(UniProt, Q9W4Y2)
Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\Pdfr for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.39

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0070436
2910
669
FBtr0308565
3230
738
FBtr0340084
3331
724
FBtr0340085
3239
724
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0099841
74.5
669
9.05
FBpp0300789
82.0
738
8.91
FBpp0309083
80.1
724
9.71
FBpp0309084
80.1
724
9.71
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

724 aa isoforms: Pdfr-PC, Pdfr-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pdfr using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Pdfr transcript is detected in the dorsolateral protocerebrum, in a region near or including the DN1 clock neurons, and in the pars intercerebralis. The cells in the dorsolateral protocerebrum are found near the dorsal projections of the s-LNv neurons.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Pdfr is expressed in two to three interneurons with somata in the posterior lateral protocerebrum that express Scer\GAL4AstA.2.74 and AstA.

Strong expression of Pdfr protein is observed in one large cell body in the dorso-lateral protocerebrum, in 20 cells in the subesophageal ganglion, in one pair of DN1, two to three DN3 neurons and in the neuronal processes throughout the brain. Weak expression is seen throughout the brain, especially in the medulla. All neuronal processes stained by Pdf protein are also stained by Pdfr protein. Some neuronal processes in the medulla and lobula only express Pdfr.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{GMR18H11-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{GMR18H11-lexA}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{Pdfr-GAL4.10}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{Pdfr-GAL4.B}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{Pdfr-myc}
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Pdfr in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
EMBL-EBI Single Cell Expression Atlas
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 23 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Pdfr
Transgenic constructs containing regulatory region of Pdfr
Aberrations (Deficiencies and Duplications) ( 8 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (19)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
5 of 15
Yes
No
3 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
Yes
1  
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (18)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
5 of 15
Yes
No
3 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (18)
3 of 13
Yes
Yes
3 of 13
Yes
No
3 of 13
Yes
Yes
3 of 13
Yes
Yes
3 of 13
Yes
Yes
3 of 13
Yes
Yes
3 of 13
Yes
Yes
3 of 13
Yes
Yes
3 of 13
Yes
Yes
3 of 13
Yes
Yes
2 of 13
No
No
2 of 13
No
No
2 of 13
No
Yes
2 of 13
No
Yes
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (28)
3 of 12
Yes
Yes
3 of 12
Yes
No
3 of 12
Yes
Yes
3 of 12
Yes
Yes
3 of 12
Yes
Yes
3 of 12
Yes
Yes
3 of 12
Yes
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (26)
4 of 15
Yes
No
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
10 of 15
Yes
Yes
3 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091903IF )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502WO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W059T )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0564 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G07WI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (18)
4 of 10
4 of 10
4 of 10
3 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 5 )
    Modifiers Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Interaction
    References
    DOES NOT ameliorate  sleep disorder
    DOES NOT ameliorate  sleep disorder
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    X
    Recombination map
    1-1
    Cytogenetic map
    Sequence location
    X:2,552,206..2,578,640 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    3A4-3A6
    Limits computationally determined from genome sequence between P{EP}CG32796EP1385&P{EP}EP1160 and P{EP}eghEP804
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (16)
    Genomic Clones (15)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (13)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: Pdfr pdfr

    Source for identity of: Pdfr CG13758

    Source for database merge of

    Source for merge of: pdfr Pdf-R1

    Source for merge of: EG:BACR25B3.3 CG13758

    Source for merge of: CG13758 anon-WO0170980.157 anon-WO0170980.158

    Additional comments

    FlyBase Curator comment: Changed symbol from 'pdfr' to 'Pdfr' so it follows our standard nomenclature rules and to make it consistent with the capitalization of the symbol of the ligand ('Pdf'). Also changed the fullname to spell out the 'PDF' abbreviation used previously.

    " EG:BACR25B3.3 " may correspond to "Scim4". Sequence analysis off ends of P{SUPor-P} in Scim insertion mutant places "Scim4" near/in " EG:BACR25B3.2 " and/or " EG:BACR25B3.3 ".

    Source for merge of EG:BACR25B3.3 CG13758 was sequence comparison ( date:000425 ).

    Source for merge of CG13758 anon-WO0170980.157 anon-WO0170980.158 was sequence comparison ( date:051113 ).

    Other Comments

    pdfr plays an important role in regulating the per oscillation and the core clock.

    pdfr does not affect Pdf expression or metabolism, but acts downstream.

    pdfr mutants display increased circadian arrhythmicity and altered geotaxis that is epistatic to that of Pdf mutants.

    Functional expression of Pdf-R1in HEK 293 cells revealed clear specificity for the Pdf protein when assayed by CRE-luciferase with an EC50 of 28 nM.

    Origin and Etymology
    Discoverer
    Etymology

    'han' refers to the Korean letter for cold.

    Identification
    External Crossreferences and Linkouts ( 58 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    EMBL-EBI Single Cell Expression Atlas
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    FlyMine - An integrated database for Drosophila genomics
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    MARRVEL_MODEL
    modMine - A data warehouse for the modENCODE project
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Synonyms and Secondary IDs (31)
    Reported As
    Symbol Synonym
    Pdf-R1
    anon-WO0170980.157
    anon-WO0170980.158
    Name Synonyms
    Groom of PDF
    PDF Receptor
    Pdf recep- tor
    Pdf receptor
    Pigment dispersing factor receptor
    Pigment dispersing factor receptor 1
    Pigment-Dispersing Factor Receptor
    Pigment-dispersing factor receptor
    groom-of-PDF
    pigment-dispersing factor receptor
    Secondary FlyBase IDs
    • FBgn0040378
    • FBgn0029622
    • FBgn0046581
    • FBgn0046582
    • FBgn0082641
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (160)