FlyBase Gene Report: Dmel\lic

General Information

Symbol

Dmel\lic

Species

D. melanogaster

Name

licorne

Annotation Symbol

CG12244

Feature Type

protein_coding_gene

FlyBase ID

FBgn0261524

Gene Model Status

Stock Availability

11 publicly available

Gene Snapshot

licorne (lic) encodes a serine/threonine kinase that phosphorylates p38 MAP kinases. [Date last reviewed: 2019-02-28]

Other Summaries

Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

MKK3, DMEK3, D-MKK3, p38, DMKK3

Key Links

Genomic Location

Cytogenetic map

Sequence location

X:13,091,145..13,093,986 [+]

Recombination map

1-41

RefSeq locus

NC_004354 REGION:13091145..13093986

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (20 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables MAP kinase kinase activity

inferred from direct assay

(Huang et al., 2016, Suzanne et al., 1999, Han et al., 1998)

enables mitogen-activated protein kinase kinase kinase binding

inferred from physical interaction with FLYBASE:Mekk1; FB:FBgn0024329

(Inoue et al., 2001)

enables protein kinase activity

inferred from direct assay

(Suzanne et al., 1999)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables MAP kinase kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000684494

(assigned by GO_Central )

(Gaudet et al., 2011)

traceable author statement

(Martin-Blanco, 2000)

Biological Process (15 terms)

Terms Based on Experimental Evidence (14 terms)

CV Term

Evidence

References

involved_in activation of MAPK activity

inferred from direct assay

(Suzanne et al., 1999, Han et al., 1998)

involved_in JNK cascade

inferred from direct assay

(Sun et al., 2019)

inferred from mutant phenotype

(Sun et al., 2019)

involved_in MAPK cascade

inferred from direct assay

(Han et al., 1998)

involved_in mucosal immune response

inferred from mutant phenotype

(Ha et al., 2009)

involved_in negative regulation of canonical Wnt signaling pathway

inferred from mutant phenotype

(Schertel et al., 2013)

involved_in negative regulation of hippo signaling

inferred from mutant phenotype

(Huang et al., 2016)

involved_in oocyte axis specification

inferred from mutant phenotype

(Suzanne et al., 1999)

involved_in p38MAPK cascade

inferred from mutant phenotype

(Chakrabarti et al., 2014)

acts_upstream_of positive regulation of cell death

inferred from genetic interaction with FLYBASE:egr; FB:FBgn0033483

(Sun et al., 2019)

involved_in positive regulation of cell size

inferred from mutant phenotype

(Cully et al., 2010)

inferred from genetic interaction with FLYBASE:gig; FB:FBgn0005198

(Cully et al., 2010)

involved_in positive regulation of multicellular organism growth

inferred from mutant phenotype

(Cully et al., 2010)

involved_in positive regulation of protein phosphorylation

inferred from genetic interaction with FLYBASE:gig; FB:FBgn0005198

(Cully et al., 2010)

involved_in regulation of adult chitin-containing cuticle pigmentation

inferred from genetic interaction with FLYBASE:Ask1; FB:FBgn0014006

(Sekine et al., 2011)

involved_in tumor necrosis factor-mediated signaling pathway

inferred from genetic interaction with FLYBASE:hep; FB:FBgn0010303

(Sun et al., 2019)

inferred from genetic interaction with FLYBASE:egr; FB:FBgn0033483

(Sun et al., 2019)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in activation of MAPK activity

inferred from biological aspect of ancestor with PANTHER:PTN000684494

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in oocyte anterior/posterior axis specification

non-traceable author statement

(Martin-Blanco, 2000)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in cytoplasm

inferred from sequence or structural similarity with SGD:S000003664

(FlyBase Curators, 2008--)

Gene Group (FlyBase)

STE7 MAP KINASE KINASES

Pathway (FlyBase)

Negative Regulators of Wnt-TCF Signaling Pathway

Negative Regulators of Hippo Signaling Pathway

TNFα-Eiger Signaling Pathway Core Components

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

STE7 MAP KINASE KINASES -: STE7 MAP kinase kinases (MAPKKs) belong to the STE7 subfamily and are serine/threonine kinases that phosphorylate MAP kinases in MAPK signalling cascades. MAPKKs are activated by MAP kinase kinase kinases (MAPKKKs). (Adapted from FBrf0225276.)

Pathway (FlyBase)

Negative Regulators of Wnt-TCF Signaling Pathway -: Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
Negative Regulators of Hippo Signaling Pathway -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).
TNFα-Eiger Signaling Pathway Core Components -: The Tumor Necrosis Factor α (TNFα) signaling pathway is activated by Eiger (egr) binding to a member of the TNF receptor superfamily. The signal is primarily transduced via the Jun N-terminal kinase (JNK) cascade, leading to cell death. (Adapted from FBrf0225608.)

Summary (Interactive Fly)

a MAP kinase kinase targeting p38 MAP kinase - mutations provoke polarity defects in the eggshell and embryo, as a result of reduced activity of two localized determinants: Oskar and Gurken

Interactive Fly

Gene Model and Products

Number of Transcripts

1

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\lic for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.50

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0073720

1951

334

Additional Transcript Data and Comments

Reported size (kB)

2.3 (northern blot)

(Suzanne et al., 1999)

1.906 (longest cDNA)

(Han et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0073551

38.2

334

6.35

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

335 (aa)

(Suzanne et al., 1999)

334 (aa)

(Han et al., 1998)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lic using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Han et al., 1998)

embryonic/larval anterior midgut

(Han et al., 1998)

embryonic/larval posterior midgut

(Han et al., 1998)

oogenesis stage S7 -- S14 & adult stage | female

(Suzanne et al., 1999)

oogenesis stage S10 -- S14 & adult stage | female

(Suzanne et al., 1999)

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- third instar larval stage

(Suzanne et al., 1999)

Additional Descriptive Data

lic transcripts are detected in embryos and larvae on northern blots. There appears to be a strong maternal contibution of transcripts to the embryo. In ovaries, lic transcripts are observed in the nurse cells from stage 7 and are transferred to the oocyte beginning in stage 10.

(Suzanne et al., 1999)

lic transcripts are detected throughout embryonic development by in situ hybridization. There is a high level of maternal deposition. Later, expression is observed in most tissues with elevated levels in the anterior and posterior midguts.

(Han et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\lic in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\lic

View exonic expression by tissue for Dmel\lic

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261524

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0015763

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12244

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of lic

Transgenic constructs containing regulatory region of lic

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation

Gene disrupted in

(Paricio et al., 1999, Suzanne et al., 1999)

(Weber et al., 2000, Paricio et al., 1999, Suzanne et al., 1999)

(Suzanne et al., 1999)

(Suzanne et al., 1999)

(Suzanne et al., 1999)

Gene duplicated in

(Popodi et al., 2010-, Venken et al., 2010)

Inferred from location

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of larval stage

lic^d13

partially lethal - majority die | female

lic^G0252

partially lethal - majority die | male

lic^G0252

partially lethal - majority die | recessive

lic^G0252

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

lic^GD7546

viable, with Scer\GAL4^Mef2.PR

lic^GD7546

viable, with Scer\GAL4^pnr-MD237

lic^GD7546

viable, with Scer\GAL4^tin.CΔ4

lic^GD7546

Sterility

Allele

female semi-sterile, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

lic^GL00022

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

lic^GL01829

Other Phenotypes

Allele

abnormal body size | third instar larval stage

lic^d13

abnormal cell migration | third instar larval stage, with Scer\GAL4^ptc.PU

lic^UAS.ORF

abnormal immune response, with Scer\GAL4^da.G32

lic^dsRNA.UAS

abnormal oxidative stress response, with Scer\GAL4^Ugt36A1.PK

lic^GD7546

decreased cell size | cell autonomous | somatic clone

lic^d13

increased cell death, with Scer\GAL4^GMR.PU

lic^UAS.ORF

increased cell death | third instar larval stage, with Scer\GAL4^ptc.PU

lic^UAS.ORF

some die during third instar larval stage

lic^d13

visible, with Scer\GAL4^Bx-MS1096

lic^UAS.ORF

visible, with Scer\GAL4^hh.PU

lic^UAS.ORF

visible | adult stage, with Scer\GAL4^pnr.PU

lic^GD7546

lic^Ubi-p63E.PS

Phenotype manifest in

Allele

adult heart, with Scer\GAL4^Ugt36A1.PK

lic^GD7546

anterior crossvein, with Scer\GAL4^Bx-MS1096

lic^UAS.ORF

anterior-posterior compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4^ptc.PU

lic^UAS.ORF

dorsal appendage

dorsal appendage | germline clone

egg | germline clone

embryo | dorsal closure stage | germline clone

embryonic/first instar larval cuticle & abdominal segment

embryonic segment

lic^{CA.sev.Tag:MYC}

eye disc, with Scer\GAL4^GMR.PU

lic^UAS.ORF

posterior crossvein, with Scer\GAL4^Bx-MS1096

lic^UAS.ORF

primordial germ cell

tergum, with Scer\GAL4^pnr.PU

lic^GD7546

wing, with Scer\GAL4^Bx-MS1096

lic^UAS.ORF

wing, with Scer\GAL4^hh.PU

lic^UAS.ORF

wing vein, with Scer\GAL4^Bx-MS1096

lic^UAS.ORF

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

12 of 15

Yes

Yes

1

11 of 15

No

Yes

1

3 of 15

No

No

2 of 15

No

No

2 of 15

No

No

Hsap\LOC100996792

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (12)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

12 of 15

Yes

Yes

11 of 15

No

Yes

3 of 15

No

No

2 of 15

No

No

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Rattus norvegicus (Norway rat) (15)

11 of 13

Yes

Yes

9 of 13

No

Yes

Rnor\LOC108348082

2 of 13

No

Yes

2 of 13

No

No

Rnor\LOC103689956

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Xenopus tropicalis (Western clawed frog) (11)

7 of 12

Yes

Yes

2 of 12

No

No

2 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

Yes

1 of 12

No

Yes

1 of 12

No

Yes

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Danio rerio (Zebrafish) (16)

10 of 15

Yes

Yes

3 of 15

No

No

2 of 15

No

No

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

Drer\si:ch211-171h4.3

1 of 15

No

No

Drer\si:dkey-8e10.3

1 of 15

No

No

1 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (9)

10 of 15

Yes

Yes

4 of 15

No

Yes

4 of 15

No

Yes

3 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

Arabidopsis thaliana (thale-cress) (10)

3 of 9

Yes

No

3 of 9

Yes

No

3 of 9

Yes

No

3 of 9

Yes

No

3 of 9

Yes

No

2 of 9

No

No

2 of 9

No

No

2 of 9

No

No

2 of 9

No

No

2 of 9

No

No

Saccharomyces cerevisiae (Brewer's yeast) (4)

5 of 15

Yes

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Schizosaccharomyces pombe (Fission yeast) (2)

5 of 12

Yes

No

1 of 12

No

No

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190AT6 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00006402

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506HN )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY001289

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_13831

Mayetiola destructor

Hessian fly

Mdes\Mdes005098

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL001622

Anopheles darlingi

American malaria mosquito

Adar\ADAC005933

Anopheles gambiae

Malaria mosquito

Agam\AGAP000310

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003157

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W06KI )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA007238

Bombyx mori

Silkmoth

Bmor\BGIBMGA005641

Danaus plexippus

Monarch butterfly

Dple\DPOGS200347-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS200381-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL000039

Heliconius melpomene

Postman butterfly

Hmel\HMEL005386

Apis florea

Little honeybee

Aflo\3089139236

Apis florea

Little honeybee

Aflo\4258277732

Apis mellifera

Western honey bee

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002360

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0018b5

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG011915

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG013714

Dendroctonus ponderosae

Mountain pine beetle

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM372730

Pediculus humanus

Human body louse

Phum\PHUM196800

Rhodnius prolixus

Kissing bug

Rpro\RPRC014923

Rhodnius prolixus

Kissing bug

Rpro\RPRC014787

Cimex lectularius

Bed bug

Clec\CLEC005853

Cimex lectularius

Bed bug

Clec\CLEC000255

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002364

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005540

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_17342

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_18358

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X06G9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR004417

Strigamia maritima

European centipede

Smar\SMAR006177

Strigamia maritima

European centipede

Smar\SMAR004780

Ixodes scapularis

Black-legged tick

Isca\ISCW023081

Ixodes scapularis

Black-legged tick

Isca\ISCW002764

Ixodes scapularis

Black-legged tick

Isca\ISCW006910

Stegodyphus mimosarum

African social velvet spider

Smim\KFM69659.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM61154.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM75651.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM75652.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM61155.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g11770

Tetranychus urticae

Two-spotted spider mite

Turt\tetur43g00210

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_322328

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_32176

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_215363

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0A9H )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000002925

Ciona intestinalis

Vase tunicate

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000002692

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (15)

Gene Symbol

Score

Source

Alignment

6 of 10

4 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 5 )

Allele

Disease

Interaction

References

ameliorates carcinoma

modeled by scrib^GD11666

(Sun et al., 2019)

modeled by Ras85D^V12.cUa.UAS, l(2)gl⁴

(Sun et al., 2019)

ameliorates carcinoma

modeled by scrib^GD11666

(Sun et al., 2019)

ameliorates carcinoma

modeled by Ras85D^V12.cUa.UAS, l(2)gl⁴

(Sun et al., 2019)

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82Q.UAS

(Park et al., 2013)

ameliorates hematologic cancer

modeled by hop^{UAS.ORF.Tag:HA}

(Terriente-Félix et al., 2017)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MAP2K6; mitogen-activated protein kinase kinase 6

12 of 15

MAP2K3; mitogen-activated protein kinase kinase 3

11 of 15

MAP2K4; mitogen-activated protein kinase kinase 4

3 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

(Sun et al., 2019)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Suganuma et al., 2010)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Belozerov et al., 2012)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

(Hu et al., 2017.6.13)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Baril et al., 2009)

suppressible

(Sun et al., 2019)

enhanceable

(Sun et al., 2019)

suppressible

(Ha et al., 2009)

suppressible

(Sun et al., 2019)

suppressible

(Sun et al., 2019)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Sekine et al., 2011)

suppressible

(Sun et al., 2019)

suppressible

(Sun et al., 2019)

enhanceable

(Sun et al., 2019)

suppressible

(Sun et al., 2019)

suppressible

(Sun et al., 2019)

suppressible

(Sekine et al., 2012)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261524

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32257

MIST (protein-protein) - An integrated Molecular Interaction Database

lic

Pathways

Signaling Pathways (FlyBase)

Negative Regulators of Wnt-TCF Signaling Pathway -: Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
Negative Regulators of Hippo Signaling Pathway -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).
TNFα-Eiger Signaling Pathway Core Components -: The Tumor Necrosis Factor α (TNFα) signaling pathway is activated by Eiger (egr) binding to a member of the TNF receptor superfamily. The signal is primarily transduced via the Jun N-terminal kinase (JNK) cascade, leading to cell death. (Adapted from FBrf0225608.)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

X

Recombination map

1-41

Cytogenetic map

Sequence location

X:13,091,145..13,093,986 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

11D10-11D10

Limits computationally determined from genome sequence between P{EP}EP1461 and P{EP}CG1640^EP1626

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

11C-11C

(determined by in situ hybridisation)

(Peter et al., 2002, Schäfer et al., 1999.6.15)

11D1-11D2

(Noselli, 1999.4.30, Noselli, 1996.4.28)

11C4-11D3

(Han, 1997.11.21)

11C4-11D3

(determined by in situ hybridisation)

(Han, 1998.4.1, Han et al., 1998)

Experimentally Determined Recombination Data

Location

1-41

(FlyBase, 2016)

1-41.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

11880

w^67c23 P{lacW}lic^G0252/FM7c

31643

y¹ v¹; P{TRiP.JF01433}attP2

60010

y¹ v¹; P{TRiP.HMS05002}attP40

19989

y¹ w^67c23 P{Mae-UAS.6.11}lic^GG01785/FM7c

53866

y¹ sc^* v¹ sev²¹; P{TRiP.GL01829}attP40

35154

y¹ sc^* v¹ sev²¹; P{TRiP.GL00022}attP2/TM3, Sb¹

F001674

M{UAS-lic.ORF}ZH-86Fb

F000883

M{UAS-lic.ORF.3xHA}ZH-86Fb

v106822

P{KK108550}VIE-260B

v20166

w¹¹¹⁸; P{GD7546}v20166/CyO

v342325

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD02100}attP40/CyO-GFP

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (260)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD04985

Other clones

DPiM_SD04985

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261524

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261524

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32257

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: lic l(1)G0252

(Schäfer, 2003.12.4)

Additional comments

May correspond to Mkk3.

(Han, 1998.4.1)

Other Comments

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G1 phase cells, a whole range of mitotic abnormalities and spindle abnormalities are seen.

(Bettencourt-Dias et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

lic is not involved in the Mekk1 regulation of the Mpk2/p38b pathway in response to environmental stresses.

(Inoue et al., 2001)

lic is required in the germline during oogenesis, for proper asymmetric development of the egg.

(Suzanne et al., 1999)

lic functions as a p38 activator suggesting that it is involved in a p38 MAPK pathway.

(Suzanne et al., 1999)

lic is required in the germline for correct asymmetric development of the egg, and may have a role in the post-transcriptional regulation of grk.

(Suzanne et al., 1999)

lic is an activator of both Mpk2 and p38b kinases.

(Han et al., 1998)

A potential activator of Mpk2, cloned by making use of a yeast osmoregulation MAPK cascade.

(Takaesu et al., 1998)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 149 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32257

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261524

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261524

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12244

FlyMine - An integrated database for Drosophila genomics

FBgn0261524

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32257

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG12244

modMine - A data warehouse for the modENCODE project

FBgn0261524

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O62602

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261524

DRSC - Results frm RNAi screens

FBgn0261524

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0015763

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32257

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (protein-protein) - An integrated Molecular Interaction Database

lic

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (23)

Reported As

Symbol Synonym

CG12244

(Rosa-Ferreira et al., 2015, Schertel et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, Brandt, 2006, Morrison et al., 2000)

D-MKK3

(Inoue et al., 2001, Han, 1998.4.1, Han et al., 1998, Han et al., 1998)

DMEK3

(Noselli et al., 1998, Suzanne et al., 1998, Suzanne et al., 1997, Noselli and Glise, 1996)

DMKK3

(Lim et al., 2014, Martin-Blanco, 2000)

DMKK3/lic

(Stronach and Perrimon, 1999)

DmMKK3

(Takaesu et al., 1998)

MEK3

(Bonke et al., 2013, Suganuma et al., 2010)

MEK3/MKK3

(Morrison et al., 2000)

MKK3

(Reboul and Ewbank, 2016, El Chamy et al., 2015, Buchon et al., 2013, Suganuma et al., 2010, Ha et al., 2009, Ha et al., 2009, Weber et al., 2000, Han, 1997.11.21, Han, 1997.11.21)

Mek3

(Noselli, 1996.4.28)

Mkk3

(Chakrabarti et al., 2014)

Mpk3

dMKK3

(Zhuang et al., 2006, Sano et al., 2005)

l(1)G0252

(Schäfer et al., 1999.6.15)

lic

(Li et al., 2020, Wang et al., 2020, Chai et al., 2019, Sun et al., 2019, Sun et al., 2019, Houtz et al., 2017, Hu et al., 2017.6.13, Jordán-Álvarez et al., 2017, Terriente-Félix et al., 2017, Huang et al., 2016, Dent et al., 2015, Bischof and FlyORF project members, 2014.6.20, Bonnay et al., 2014, Sopko et al., 2014, Bonke et al., 2013, Carter, 2013, Kwon et al., 2013, Kwon et al., 2013, Park et al., 2013, Schertel et al., 2013, Schertel et al., 2013, Gonda et al., 2012, Sekine et al., 2012, Friedman et al., 2011, Sekine et al., 2011, Chen et al., 2010, Cobreros-Reguera et al., 2010, Cully et al., 2010, Popodi et al., 2010-, Venken et al., 2010, Zhang et al., 2010, Baril et al., 2009, Peretz et al., 2007, Walther and Pichaud, 2007, Zeitlinger et al., 2007, Brandt, 2006, Guichard et al., 2006, Haddrill et al., 2005, Terashima and Bownes, 2005)

p38

(Hwang et al., 2019, Santabárbara-Ruiz et al., 2015, Giot et al., 2003)

p38MAPKK

(Han, 1998.1.4)

Name Synonyms

Drosophila MAP kinase kinase 3

(Han et al., 1998)

Licorne

(Igboin et al., 2012, Guichard et al., 2006)

MAP kinase kinase 3

(Han, 1997.11.21)

MEK-kinase 3

(Noselli and Glise, 1996)

Mkk3

licorne

(Yang et al., 2018, Bonke et al., 2013, Gomez et al., 2012, Seisenbacher et al., 2011, Sekine et al., 2011, Cully et al., 2010, Walther and Pichaud, 2007, Haddrill et al., 2005, Terashima and Bownes, 2005, Noselli, 1999.4.30)

p38 MAPK

(Grill et al., 2016)

Secondary FlyBase IDs

FBgn0015763
FBgn0024952
FBgn0027251
FBgn0024327

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (135)