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General Information
Symbol
Dmel\RpS6
Species
D. melanogaster
Name
Ribosomal protein S6
Annotation Symbol
CG10944
Feature Type
FlyBase ID
FBgn0261592
Gene Model Status
Stock Availability
Gene Summary
Ribosomal protein S6 (RpS6) encodes a key component of the small (40S) ribosomal subunit. [Date last reviewed: 2019-02-28] (FlyBase Gene Snapshot)
Also Known As

S6, air8, hen, l(1)air8, M(1)7BC

Key Links
Genomic Location
Cytogenetic map
Sequence location
X:7,899,624..7,901,331 [-]
Recombination map
1-21
RefSeq locus
NC_004354 REGION:7899624..7901331
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
Biological Process (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
traceable author statement
involved_in translation
inferred from electronic annotation with InterPro:IPR001377, InterPro:IPR014401, InterPro:IPR018282
(assigned by InterPro )
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
traceable author statement
Protein Family (UniProt)
Belongs to the eukaryotic ribosomal protein eS6 family. (P29327)
Summaries
Gene Snapshot
Ribosomal protein S6 (RpS6) encodes a key component of the small (40S) ribosomal subunit. [Date last reviewed: 2019-02-28]
Gene Group (FlyBase)
CYTOPLASMIC SMALL RIBOSOMAL PROTEINS -
Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytoplasm. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the small subunit is to bind mRNA. (Adapted from FBrf0205398)
Protein Function (UniProtKB)
Component of the 40S small ribosomal subunit (By similarity). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (By similarity).
(UniProt, P29327)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\RpS6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0071136
1195
217
FBtr0071135
960
248
Additional Transcript Data and Comments
Reported size (kB)

1.1 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0071088
24.7
217
11.70
FBpp0071087
28.4
248
11.43
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Post Translational Modification

Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.

(UniProt, P29327)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpS6 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

Additional Descriptive Data

Mutant RpS6 transcripts are barely detectable on northern blots of @RpS6air8 RNA.

RpS6 transcripts are abundant throughout development.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from high throughput direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\RpS6 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
EMBL-EBI Single Cell Expression Atlas
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of RpS6
Transgenic constructs containing regulatory region of RpS6
Aberrations (Deficiencies and Duplications) ( 9 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
2  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
7 of 15
No
Yes
2 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
12 of 13
Yes
Yes
5 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
13 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
14 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
13 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
9 of 9
Yes
Yes
9 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
12 of 15
Yes
Yes
9 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
11 of 12
Yes
Yes
11 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190F4F )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915046T )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles darlingi
American malaria mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02S2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02P5 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G089B )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    RNA-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    X
    Recombination map
    1-21
    Cytogenetic map
    Sequence location
    X:7,899,624..7,901,331 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    7C1-7C2
    Left limit from inclusion within Df(1)hl-c (FBrf0052091) Right limit from inclusion within Df(1)hl-a (FBrf0052091); Limits computationally determined from genome sequence between P{EP}EP1209 and P{EP}CG10777EP1347&P{EP}CG10777EP1363
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    7C-7C
    (determined by in situ hybridisation) 7C5--D1 (determined by in situ hybridisation)
    7C-7C
    (determined by in situ hybridisation)
    7C3-7D2
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Notes
    Stocks and Reagents
    Stocks (3)
    Genomic Clones (14)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (395)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: RpS6 CG10944

    Source for database merge of

    Source for merge of: RpS6 M(1)7BC M(1)7C

    Source for merge of: RpS6 anon-WO02059370.61

    Additional comments

    Source for merge of RpS6 anon-WO02059370.61 was sequence comparison ( date:051113 ).

    Other Comments

    Haploinsufficient locus.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

    Minute gene.

    Deletions removing RpS6 but no other cytoplasmic ribosomal protein-encoding genes show Minute phenotypes.

    Molecularly-defined mutations in RpS6 result in Minute phenotypes.

    Expression of transcript changes with adult age.

    Mutants display a blood cell neoplastic phenotype.

    Isolated from a Drosophila embryonic cDNA library using a human S6 cDNA as a probe, under intermediate stringency conditions.

    An RpS6 cDNA has been cloned and sequenced.

    Comparison of cDNA with genomic DNA identifies a transcription unit including three exons. Two tandem repeats downstream of this transcription unit reiterate divergent copies of the third exon and flanking regions. Comparison of these three repeats with respect to nucleotide sequence shows they clearly arose via a duplication and subsequent crossing over between misaligned copies. Although no direct evidence exists that the downstream exons are transcribed the maintenance of open reading frames in spite of extensive genetic changes is consistent with a protein-coding function.

    Genetic and molecular analysis of RpS6 and phenotypic characterisation of two lethal mutations which result in hypertrophy of the larval haematopoietic organs because of overproliferation and aberrant development of haemocytes.

    When mutated RpS6 causes overproliferation of the larval haemopoietic cells, tumour formation and an increase in the nuclear DNA content of haemopoietic cells.

    Mutations cause overgrowth of lymph glands, abnormal blood cell differentiation, melanotic tumour formation, delayed larval growth, larval organ development retardation and larval death. Clonal analysis reveals wild type RpS6 required for clone survival in germ line and imaginal discs. Regulatory role of RpS6 in the hematopoeitic system may be related to its developmentally regulated phosphorylation.

    A deficiency chromosome that removes RpS14A and RpS14B does not display the Minute phenotype associated with the M(1)7C locus: RpS14A and RpS14B do not correspond to the M(1)7C locus.

    Mutations that exhibit melanotic tumor phenotypes have been initially characterized.

    Deficiency mapping suggests that M(1)7C, a novel minute locus, is in the same region as RpS14A and RpS14B.

    Dysgenic revertants were generated using jumpstarter mutagenesis for P-element excision.

    One of a class of genes (see MIN record) that when present in one, rather than two, copies, produce a characteristic phenotype consisting of short slender bristles and delayed development.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 66 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    EMBL-EBI Single Cell Expression Atlas
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    FlyMine - An integrated database for Drosophila genomics
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    MARRVEL_MODEL
    modMine - A data warehouse for the modENCODE project
    Synonyms and Secondary IDs (30)
    Reported As
    Symbol Synonym
    anon-WO02059370.61
    l(1)air8
    l(1)hen
    Name Synonyms
    Minute
    Minute (1) 7BC
    Ribosomal protein S6
    S6 ribosomal protein
    hemocytes enlarged
    lethal(1)aberrant immune response8
    lethal-(1)-haemocytes enlarged
    lethal-aberrant-immune-response-8
    Secondary FlyBase IDs
    • FBgn0004922
    • FBgn0004371
    • FBgn0004388
    • FBgn0065997
    • FBgn0005276
    • FBgn0004405
    • FBgn0002584
    • FBgn0002585
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (150)