FlyBase Gene Report: Dmel\nej

General Information

Symbol

Dmel\nej

Species

D. melanogaster

Name

nejire

Annotation Symbol

CG15319

Feature Type

protein_coding_gene

FlyBase ID

FBgn0261617

Gene Model Status

Current

Stock Availability

29 publicly available

Enzyme Name (EC)

Histone acetyltransferase (2.3.1.48)

Gene Summary

nejire (nej) encodes the transcriptional co-activator CBP. It acetylates several nuclear proteins, including the histone encoded by His3 on K18, K27, and H4 on K8. By regulating gene expression, the product of nej has roles in cell proliferation, cell signaling and differentiation, and in developmental patterning. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

CBP, dCBP, CREB binding protein, CREB-binding protein, CBP/p300

Key Links

Genomic Location

Cytogenetic map

8F7-8F9

Sequence location

X:9,665,442..9,687,066 [-]

Recombination map

1-29

RefSeq locus

NC_004354 REGION:9665442..9687066

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (43 terms)

Molecular Function (11 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

enables histone acetyltransferase activity (H3-K18 specific)

inferred from direct assay

(Tie et al., 2009)

enables histone acetyltransferase activity (H3-K27 specific)

inferred from direct assay

(Tie et al., 2009)

enables histone acetyltransferase activity

inferred from direct assay

(Petruk et al., 2003, Ludlam et al., 2002)

enables histone methyltransferase binding

inferred from physical interaction with FLYBASE:ash1; FB:FBgn0005386

(Bantignies et al., 2000)

enables protein binding

inferred from physical interaction with FLYBASE:Clk; FB:FBgn0023076

(Hung et al., 2007)

inferred from physical interaction with FLYBASE:mod; FB:FBgn0002780

(Bantignies et al., 2002)

enables RNA polymerase II-specific DNA-binding transcription factor binding

inferred from physical interaction with UniProtKB:P19538

(Han et al., 2015)

enables transcription coactivator activity

inferred from direct assay

(Hung et al., 2007)

inferred from genetic interaction with FLYBASE:per; FB:FBgn0003068

(Hung et al., 2007)

inferred from genetic interaction with FLYBASE:ci; FB:FBgn0004859

(Chen et al., 2000)

enables transcription factor binding

inferred from physical interaction with FLYBASE:ci; FB:FBgn0004859

(Chen et al., 2000, Akimaru et al., 1997)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables cAMP response element binding protein binding

traceable author statement

(Marek et al., 2000)

enables chromatin DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000351795

(assigned by GO_Central )

(Gaudet et al., 2011)

enables histone acetyltransferase activity

inferred from biological aspect of ancestor with PANTHER:PTN000351795

(assigned by GO_Central )

(Gaudet et al., 2011)

enables transcription coactivator activity

inferred from biological aspect of ancestor with PANTHER:PTN000351795

(assigned by GO_Central )

(Gaudet et al., 2011)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR000197, InterPro:IPR000433

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (26 terms)

Terms Based on Experimental Evidence (23 terms)

CV Term

Evidence

References

involved_in circadian regulation of gene expression

inferred from mutant phenotype

(Hung et al., 2007)

involved_in compound eye development

inferred from mutant phenotype

(Mallik and Lakhotia, 2010)

involved_in compound eye morphogenesis

inferred from mutant phenotype

(Kumar et al., 2004)

involved_in DNA replication checkpoint signaling

inferred from mutant phenotype

(Smolik and Jones, 2007)

involved_in glial cell migration

inferred from mutant phenotype

(Schmidt et al., 2011)

involved_in histone acetylation

inferred from direct assay

(Petruk et al., 2003)

involved_in histone H3-K18 acetylation

inferred from direct assay

(Tie et al., 2009)

involved_in histone H3-K27 acetylation

inferred from direct assay

(Tie et al., 2009)

involved_in histone H3-K4 methylation

inferred from direct assay

(Petruk et al., 2003)

involved_in histone H4-K12 acetylation

inferred from mutant phenotype

(Ludlam et al., 2002)

involved_in histone H4-K8 acetylation

inferred from mutant phenotype

(Boyles et al., 2010, Ludlam et al., 2002)

involved_in locomotor rhythm

inferred from mutant phenotype

(Hung et al., 2007)

involved_in negative regulation of canonical Wnt signaling pathway

inferred from mutant phenotype

(Li et al., 2007)

involved_in neurotransmitter secretion

inferred from mutant phenotype

(Marek et al., 2000)

involved_in ovarian follicle cell development

inferred from mutant phenotype

(Jia et al., 2015)

involved_in positive regulation of canonical Wnt signaling pathway

inferred from mutant phenotype

(Li et al., 2007)

involved_in positive regulation of smoothened signaling pathway

inferred from genetic interaction with FLYBASE:ci; FB:FBgn0004859

(Akimaru et al., 1997)

inferred from mutant phenotype

(Chen et al., 2000)

involved_in positive regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Chen et al., 2000)

inferred from genetic interaction with FLYBASE:ci; FB:FBgn0004859

(Chen et al., 2000)

involved_in R3/R4 cell fate commitment

inferred from mutant phenotype

(Kumar et al., 2004)

involved_in R7 cell differentiation

inferred from mutant phenotype

(Kumar et al., 2004)

involved_in regulation of mitotic nuclear division

inferred from mutant phenotype

(Fung et al., 2003)

inferred from genetic interaction with FLYBASE:Myb; FB:FBgn0002914

(Fung et al., 2003)

involved_in synapse assembly

inferred from mutant phenotype

(Marek et al., 2000)

involved_in thermosensory behavior

inferred from mutant phenotype

(Hong et al., 2008)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in hemopoiesis

traceable author statement

(Evans et al., 2003)

involved_in histone acetylation

inferred from biological aspect of ancestor with PANTHER:PTN000351795

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in positive regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000351795

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in smoothened signaling pathway

non-traceable author statement

(Fry and Peterson, 2001)

involved_in Wnt signaling pathway

non-traceable author statement

(Fry and Peterson, 2001)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

part_of histone acetyltransferase complex

inferred from direct assay

(Petruk et al., 2003)

located_in nucleoplasm

inferred from high throughput direct assay

(Lye et al., 2014)

located_in nucleus

inferred from direct assay

(Bantignies et al., 2002, Marek et al., 2000)

located_in polytene chromosome

inferred from direct assay

(Petruk et al., 2001, Bantignies et al., 2000)

part_of protein-containing complex

inferred from physical interaction with FLYBASE:mod; FB:FBgn0002780

(Bantignies et al., 2002)

inferred from physical interaction with FLYBASE:ash1; FB:FBgn0005386

(Bantignies et al., 2000)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of histone acetyltransferase complex

inferred from biological aspect of ancestor with PANTHER:PTN000351795

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of transcription regulator complex

inferred from biological aspect of ancestor with PANTHER:PTN000351795

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

TRITHORAX ACETYLATION COMPLEX 1

HISTONE ACETYLTRANSFERASES

Pathway (FlyBase)

Negative Regulators of Wnt-TCF Signaling Pathway

Positive Regulators of Wnt-TCF Signaling Pathway

Positive Regulators of Hedgehog Signaling Pathway

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine (2.3.1.48)

Predictions / Assertions

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine (2.3.1.48)

Summaries

Gene Snapshot

Gene Group (FlyBase)

TRITHORAX ACETYLATION COMPLEX 1 -: The Trithorax Acetylation Complex 1 (TAC1) possesses histone H3 K4 methyltransferase and histone acetyltransferase activities. (Adapted from FBrf0192625 and FBrf0167942).
HISTONE ACETYLTRANSFERASES -: Histone acetyltransferases catalyze the acetylation of conserved lysine residues on histone proteins by transferring an acetyl group from acetyl CoA to form ε-N-acetyl lysine. (Adapted from PMID:11395403).

Pathway (FlyBase)

Negative Regulators of Wnt-TCF Signaling Pathway -: Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
Positive Regulators of Wnt-TCF Signaling Pathway -: Positive regulators of Wnt-TCF (canonical Wnt) signaling up-regulate the pathway, resulting in the enhancement of transcriptional regulation mediated by β-catenin (arm).
Positive Regulators of Hedgehog Signaling Pathway -: Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.

Summary (Interactive Fly)

CBP, a transcriptional co-activator that interacts with a large number of developmentally important transcription factors - acetylates several nuclear proteins, including histone H3 on K18, K27, and H4 on K8. - roles in cell proliferation, cell signaling and differentiation, and in developmental patterning

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\nej for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Stage-specific extension of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated.

Gene model reviewed during 5.43

Gene model reviewed during 5.42

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

nej-RB

FBtr0071402

NM_079903

11733

3276

nej-RC

FBtr0302722

NM_001201646

11704

3282

nej-RD

FBtr0302723

NM_001201647

15729

3276

nej-RE

FBtr0333521

NM_001272458

15400

3266

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

nej-PB

FBpp0071337

340.7

3276

8.86

NP_524642

AAF46516

nej-PC

FBpp0291862

341.4

3282

8.89

NP_001188575

ADV37657

nej-PD

FBpp0291863

340.7

3276

8.86

NP_001188576

ADV37658

nej-PE

FBpp0305701

339.7

3266

8.86

NP_001259387

AGB95230

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

3276 aa isoforms: nej-PB, nej-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

3190 (aa); 332 (kD)

(Akimaru et al., 1997)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nej using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 17

organism

Comment: extended 3' UTR isoform

(Hilgers et al., 2011)

embryonic stage 12 -- 15

embryonic/larval nervous system

Comment: extended 3' UTR isoform

(Hilgers et al., 2011)

Additional Descriptive Data

Zygotic-specific isoforms of nej with long 3' UTR extensions were observed. The 3' UTR extension isoforms are highly enriched in nervous system tissues.

(Hilgers et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 12

organism | ubiquitous

(Ludlam et al., 2002)

embryonic stage 5

• nucleus

(Lilja et al., 2003)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of histone acetyltransferase complex

inferred from direct assay

(Petruk et al., 2003)

located_in nucleoplasm

inferred from high throughput direct assay

(Lye et al., 2014)

located_in nucleus

inferred from direct assay

(Bantignies et al., 2002, Marek et al., 2000)

located_in polytene chromosome

inferred from direct assay

(Petruk et al., 2001, Bantignies et al., 2000)

part_of protein-containing complex

inferred from physical interaction with FLYBASE:mod; FB:FBgn0002780

(Bantignies et al., 2002)

inferred from physical interaction with FLYBASE:ash1; FB:FBgn0005386

(Bantignies et al., 2000)

Expression Deduced from Reporters

Reporter: PBac{602.P.SVS-1}nej^CPTI000727

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\nej in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\nej

View exonic expression by tissue for Dmel\nej

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB

43856/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0261617

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15319

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 33 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 32 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of nej

Transgenic constructs containing regulatory region of nej

Aberrations (Deficiencies and Duplications) ( 10 )

Inferred from experimentation ( 10 )

Gene not duplicated in

Dp(1;2)v⁺75d

(Florence and McGinnis, 1998)

Dp(1;3)w^m49a

(Florence and McGinnis, 1998)

Gene not disrupted in

Df(1)9a4-5

(Florence and McGinnis, 1998)

Df(1)dm75e19

(Florence and McGinnis, 1998)

Df(1)lz-90b24

(Florence and McGinnis, 1998)

Df(1)v-L15

(Florence and McGinnis, 1998)

Gene duplicated in

Dp(1;3)DC212

(Popodi et al., 2010-, Venken et al., 2010)

Dp(1;Y)FF1

(Florence and McGinnis, 1998)

Dp(1;Y)lz⁺

(Florence and McGinnis, 1998)

Gene disrupted in

Df(1)C52

(Florence and McGinnis, 1998)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal, with Scer\GAL4^pnr-MD237

nej^GD1494

lethal (with nej³)

nej^P

lethal (with nej^P)

lethal (with nej^Q7)

nej^P

lethal (with nej^S103)

nej^P

lethal (with nej^S342)

nej^P

lethal (with nej^TA57)

nej^P

lethal | adult stage

nej^G0112

lethal | pharate adult stage

nej^G0112

lethal | pharate adult stage, with Scer\GAL4^30A

nej^UAS.cMa

lethal | pupal stage, with Scer\GAL4^Act5C.PI

nej^{dsRNA.UAS.cKa}

lethal | recessive

lethal - all die before end of larval stage, with Scer\GAL4^elav.PLu

nej^EP1149

lethal - all die before end of prepupal stage

nej^G0470

lethal - all die before end of prepupal stage, with Scer\GAL4^GMR.PF

nej^ΔQ.UAS

lethal - all die before end of P-stage

lethal - all die before end of pupal stage, with Scer\GAL4^GMR.PF

nej^ΔQ.UAS

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

nej^GD8975

lethal - all die during embryonic stage | recessive

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

nej^GD1494

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

nej^GD8975

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

nej^GD1494

partially lethal - majority die, with Scer\GAL4^OK363

nej^UAS.cMa

partially lethal - majority die | embryonic stage, with Scer\GAL4^Act5C.PI

nej^{dsRNA.UAS.cKa}

partially lethal - majority die | larval stage, with Scer\GAL4^Act5C.PI

nej^{dsRNA.UAS.cKa}

partially lethal - majority die | pharate adult stage, with Scer\GAL4^30A

nej^UAS.cMa

partially lethal - majority die | recessive

partially lethal - majority live

viable, with Scer\GAL4^- :

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

nej^GD8975

viable, with Scer\GAL4^tin.CΔ4

nej^GD1494

Sterility

Allele

female fertile, with Scer\GAL4^nos.PG

male sterile

nej^G0350

Other Phenotypes

Allele

abnormal cell migration

nej^XII-13D1

abnormal courtship behavior | conditional, with Scer\GAL4^Or47b.7.467

nej^dsRNA.UAS

abnormal developmental rate | recessive

abnormal developmental rate | recessive, with Scer\GAL4^- :

abnormal eye color, with Scer\GAL4^GMR.PF

nej^{dsRNA.UAS.cKa}

abnormal locomotor behavior | adult stage | heat sensitive, with Scer\GAL4^hs.PU

nej^{dsRNA.UAS.cKa}

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^hs.PB

nej^dsRNA.UAS

abnormal neuroanatomy

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

abnormal neuroanatomy | larval stage

nej^GD8975

abnormal neuroanatomy | P-stage, with Scer\GAL4^ppk.PU

nej^GD1494

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^GMR.PU

nej^ΔBHQ.UAS

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^ppk.PU

nej^HMS01507

abnormal neurophysiology

abnormal neurophysiology, with Scer\GAL4^elav.PLu

abnormal neurophysiology, with Scer\GAL4^Mhc.PW

nej^UAS.cMa

abnormal neurophysiology | adult stage | conditional, with Scer\GAL4^Or47b.7.467

nej^dsRNA.UAS

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

nej^GD8975

abnormal size | adult stage, with Scer\GAL4^GMR.PU

nej^ΔBHQ.UAS

chemical sensitive | dominant

nej³

hypoactive | recessive

hypoactive | recessive, with Scer\GAL4^- :

some die during embryonic stage | recessive

some die during larval stage, with Scer\GAL4^elav.PLu

nej^EP1149

some die during pharate adult stage, with Scer\GAL4^OK363

nej^UAS.cMa

some die during prepupal stage | recessive

nej^G0470

some die during prepupal stage | recessive, with Scer\GAL4^GMR.PF

nej^ΔQ.UAS

some die during P-stage

some die during pupal stage, with Scer\GAL4^GMR.PF

nej^ΔQ.UAS

some die during pupal stage, with Scer\GAL4^pnr-MD237

nej^GD8975

visible

nej^UAS.cMa

visible, with Scer\GAL4^30A

nej^UAS.cMa

visible, with Scer\GAL4^bbg-C96

visible, with Scer\GAL4^ey.PH

visible, with Scer\GAL4^GMR.PF

visible, with Scer\GAL4^GMR.PF, nej³

visible, with Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS

nej³

visible, with Scer\GAL4^GMR.PF, nej^ΔNZK.UAS

nej³

visible, with Scer\GAL4^GMR.PU

nej^UAS.cMa

visible, with Scer\GAL4^hs.2sev

visible, with Scer\GAL4^hs.PB

nej^{dsRNA.UAS.cKa}

visible, with Scer\GAL4^OK363

nej^UAS.cMa

visible, with Scer\GAL4^OK386

nej^UAS.cMa

visible, with Scer\GAL4^pnr-MD237

nej^{dsRNA.UAS.cKa}

visible, with Scer\GAL4^sca-537.4

nej^EP1149

visible (with nej¹³¹)

nej^P

visible (with nej^P)

visible (with nej^TC41)

nej^P

visible | adult stage, with Scer\GAL4^en.PU

nej^JF02806

visible | adult stage, with Scer\GAL4^ey.PH

nej^JF02806

visible | adult stage, with Scer\GAL4^GMR.PF

nej^JF02806

visible | adult stage, with Scer\GAL4^nub.PK

nej^JF02806

visible | recessive | somatic clone

nej^TC41

visible | somatic clone

nej^8B27

wild-type

nej^hs.PA

Phenotype manifest in

Allele

abdominal 4 ventral denticle belt

nej¹

abdominal 5 ventral denticle belt

nej¹

adult abdominal segment 4 | ectopic, with Scer\GAL4^hs.PB

nej^{dsRNA.UAS.cKa}

adult abdominal segment 5, with Scer\GAL4^hs.PB

nej^{dsRNA.UAS.cKa}

adult olfactory receptor neuron Or47b | adult stage | conditional, with Scer\GAL4^Or47b.7.467

nej^dsRNA.UAS

amnioserosa | germline clone

nej¹

axon | third instar larval stage, with Scer\GAL4^GMR.PU

nej^ΔBHQ.UAS

cephalopharyngeal skeleton

nej³

condensed chromosome | third instar larval stage, with Scer\GAL4^hs.PB

nej^dsRNA.UAS

cone cell | ectopic, with Scer\GAL4^GMR.PF

cuticle

nej³

dendritic tree | P-stage, with Scer\GAL4^ppk.PU

nej^GD1494

denticle belt | anterior

nej³

dorsocentral bristle, with Scer\GAL4^sca-537.4

nej^EP1149

ectoderm | dorsal | germline clone

nej¹

embryonic/first instar larval cuticle

nej¹

embryonic/larval dorsal trunk

nej³

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

nej^HMS01507

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

embryonic/larval midgut | embryonic stage

nej³

embryonic/larval midgut | embryonic stage | germline clone

nej¹

embryonic/larval proventriculus | embryonic stage

embryonic/larval salivary gland

embryonic/larval salivary gland, with Scer\GAL4^SG

nej^{dsRNA.UAS.cYa}

embryonic/larval tracheal system

nej³

embryonic/larval ventral anastomosis

nej³

embryonic antennal sense organ

embryonic head

nej¹

embryonic midgut constriction 1

nej³

epidermis

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

nej^HMS01507

eye

nej^UAS.cMa

eye, with Scer\GAL4^ey.PH

eye, with Scer\GAL4^GMR.PF

eye, with Scer\GAL4^GMR.PF, nej³

eye, with Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS

nej³

eye, with Scer\GAL4^GMR.PF, nej^ΔNZK.UAS

nej³

eye, with Scer\GAL4^GMR.PU

eye, with Scer\GAL4^hs.2sev

eye (with nej¹³¹)

nej^P

eye (with nej^P)

eye (with nej^TC41)

nej^P

eye | dorsal, with Scer\GAL4^ey.PH

nej^ΔNZK.UAS

eye | somatic clone

eye disc, with Scer\GAL4^ey.PH

nej^ΔQ.UAS

eye disc, with Scer\GAL4^GMR.PF

eye disc | somatic clone | third instar larval stage

nej^8B27

eye disc | third instar larval stage, with Scer\GAL4^hs.PB

nej^dsRNA.UAS

eye photoreceptor cell, with Scer\GAL4^GMR.PF

eye photoreceptor cell, with Scer\GAL4^hs.2sev

nej^ΔQ.UAS

eye photoreceptor cell (with nej¹³¹)

nej^P

eye photoreceptor cell (with nej^P)

nej¹³¹

eye photoreceptor cell | ectopic, with Scer\GAL4^GMR.PF

nej^ΔNZK.UAS

eye photoreceptor cell | somatic clone

nej^TC41

eye photoreceptor cell | third instar larval stage, with Scer\GAL4^GMR.PU

nej^ΔBHQ.UAS

filzkorper

nej³

ganglion mother cell | larval stage

nej^GD8975

gastric caecum

nej³

head

interommatidial bristle, with Scer\GAL4^GMR.PF

interommatidial bristle, with Scer\GAL4^hs.2sev

nej^ΔBHQ.UAS

lamellocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

nej^UAS.Tag:V5

larval dorsal multidendritic neuron ddaC | P-stage, with Scer\GAL4^ppk.PU

nej^GD1494

larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4^ppk.PU

nej^HMS01507

macrochaeta

nej³

median tooth

mouth hook

mystery cell, with Scer\GAL4^GMR.PF

nej^ΔNZK.UAS

neuromuscular junction

neuromuscular junction, with Scer\GAL4^- :

neuromuscular junction, with Scer\GAL4^elav.PLu

neuromuscular junction, with Scer\GAL4^Mhc.PW

nej^UAS.cMa

neuromuscular junction, with Scer\GAL4^Mhc.PW, Scer\GAL4^elav.PLu

nej^EP1179

neuromuscular junction & bouton

neuromuscular junction & bouton, with Scer\GAL4^- :

neuromuscular junction & bouton, with Scer\GAL4^elav.PLu

nej^EP1179

neuromuscular junction & t-bar, with Scer\GAL4^- :

nej^EP1149

ommatidial precursor cluster, with Scer\GAL4^GMR.PF

ommatidium, with Scer\GAL4^ey.PH

nej^{dsRNA.UAS.cKa}

ommatidium, with Scer\GAL4^GMR.PF

ommatidium, with Scer\GAL4^hs.2sev

ommatidium (with nej¹³¹)

nej^P

ommatidium (with nej^P)

ommatidium (with nej^TC41)

nej^P

ommatidium | somatic clone

nej^TC41

outer photoreceptor cell | ectopic, with Scer\GAL4^hs.2sev

nej^KIX.UAS

photoreceptor cell R3, with Scer\GAL4^hs.2sev

photoreceptor cell R3 | ectopic, with Scer\GAL4^hs.2sev

photoreceptor cell R4, with Scer\GAL4^hs.2sev

photoreceptor cell R7, with Scer\GAL4^hs.2sev

photoreceptor cell R7 | ectopic, with Scer\GAL4^hs.2sev

pigment cell | somatic clone

nej^TC41

retina, with Scer\GAL4^GMR.PF

nej^UAS.Tag:V5

rhabdomere, with Scer\GAL4^GMR.PF

scutellar bristle, with Scer\GAL4^pnr-MD237

nej^{dsRNA.UAS.cKa}

scutellar bristle, with Scer\GAL4^sca-537.4

nej^EP1149

subperineurial glial cell

nej^XII-13D1

terminal organ | embryonic stage

thorax

tormogen cell, with Scer\GAL4^sca-537.4

nej^EP1149

trichogen cell, with Scer\GAL4^sca-537.4

nej^EP1149

wing, with Scer\GAL4^30A

nej^UAS.cMa

wing, with Scer\GAL4^bbg-C96

nej^UAS.Tag:V5

wing, with Scer\GAL4^OK363

nej^UAS.cMa

wing, with Scer\GAL4^OK386

nej^UAS.cMa

wing blade, with Scer\GAL4^bbg-C96

nej^{dsRNA.UAS.cKa}

wing blade, with Scer\GAL4^en.PU

nej^JF02806

wing blade, with Scer\GAL4^nub.PK

nej^JF02806

wing margin, with Scer\GAL4^bbg-C96

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (17)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CREBBP

12 of 15

Yes

Hsap\EP300

12 of 15

Yes

Hsap\MIR1281

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (17)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Crebbp

11 of 15

Yes

Mmus\Ep300

11 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (12)

Rnor\Crebbp

8 of 13

Yes

Rnor\Ep300

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (16)

Xtro\crebbp

5 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Xtro\kat2a

1 of 12

Xtro\kat2b

1 of 12

Xtro\pbrm1

1 of 12

Danio rerio (Zebrafish) (20)

Drer\crebbpa

10 of 15

Yes

Drer\crebbpb

10 of 15

Yes

Drer\ep300a

6 of 15

Yes

Drer\ep300b

6 of 15

Yes

Drer\crebbp

3 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Drer\si:ch211-59d17.3

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (17)

Cele\cbp-1

12 of 15

Yes

Cele\C29F9.5

5 of 15

Yes

Cele\cbp-3

4 of 15

Yes

Cele\C29F9.6

3 of 15

Yes

Cele\C49F5.5

3 of 15

Yes

Cele\cbp-2

3 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Cele\F13C5.2

1 of 15

Cele\F22F1.3

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Cele\ZK1010.10

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (5)

Atha\HAC1

6 of 9

Yes

Atha\HAC4

6 of 9

Yes

Atha\HAC5

6 of 9

Yes

Atha\HAC12

6 of 9

Yes

Atha\HAC2

4 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (10)

Scer\BDF1

1 of 15

Yes

Scer\BDF2

1 of 15

Yes

Scer\GCN5

1 of 15

Yes

Scer\ITC1

1 of 15

Yes

Scer\RSC1

1 of 15

Yes

Scer\RSC2

1 of 15

Yes

Scer\RSC4

1 of 15

Yes

Scer\SLA1

1 of 15

Yes

Scer\SPT7

1 of 15

Yes

Scer\YPL216W

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\shd1

1 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900IP )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA13644

Drosophila persimilis

Dper\GL16612

Drosophila willistoni

Dwil\GK25575

Drosophila virilis

Dvir\GJ19060

Drosophila mojavensis

Dmoj\GI14627

Drosophila grimshawi

Dgri\GH12924

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915006R )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17021269

Glossina morsitans

Tsetse fly

Gmor\GMOY006377

Mayetiola destructor

Hessian fly

Mdes\Mdes012211

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL017391

Anopheles darlingi

American malaria mosquito

Adar\ADAC008240

Anopheles gambiae

Malaria mosquito

Agam\AGAP000029

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ005540

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W005E )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA000853

Danaus plexippus

Monarch butterfly

Dple\DPOGS202654-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL002224

Apis florea

Little honeybee

Aflo\2232656676

Apis mellifera

Western honey bee

Amel\GB51769

Apis mellifera

Western honey bee

Amel\GB55583

Apis mellifera

Western honey bee

Amel\GB55582

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP20495

Bombus terrestris

Buff-tailed bumblebee

Bter\15203383

Linepithema humile

Argentine ant

Lhum\LH14963

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000ada

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004485

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_04630

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_00447

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_08222

Pediculus humanus

Human body louse

Phum\PHUM086710

Cimex lectularius

Bed bug

Clec\CLEC025136

Cimex lectularius

Bed bug

Clec\CLEC025136-PB

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003301

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08401

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0057 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR008295

Ixodes scapularis

Black-legged tick

Isca\ISCW021823

Ixodes scapularis

Black-legged tick

Isca\ISCW021822

Stegodyphus mimosarum

African social velvet spider

Smim\KFM79838.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM67729.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur07g03920

Tetranychus urticae

Two-spotted spider mite

Turt\tetur07g03900

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_64953

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_203585

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_9307

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0L04 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cbp

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000009030

Gallus gallus

Domestic chicken

Ggal\EP300

Gallus gallus

Domestic chicken

Ggal\CREBBP

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (11)

Gene Symbol

Score

Source

Alignment

Acf

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Lpt

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

tou

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 3 )

Allele

Disease

Evidence

References

nej^Q7

model of alcohol use disorder

CEA

(Ghezzi et al., 2017)

nej³

model of alcohol use disorder

CEA

(Ghezzi et al., 2017)

nej^hs.PA

model of alcohol use disorder

CEA

(Ghezzi et al., 2017)

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

CREBBP; CREB binding protein

model of Rubinstein-Taybi syndrome

IEA

(FlyBase, 2019-)

EP300; E1A binding protein p300

model of Rubinstein-Taybi syndrome

IEA

(FlyBase, 2019-)

model of colorectal cancer

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 15 )

Allele

Disease

Interaction

References

nej^Q7

ameliorates Schimke immuno-osseous dysplasia

modeled by Marcal1^UAS.cBa

(Morimoto et al., 2016)

nej³

exacerbates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Bodai et al., 2012)

nej^UAS.Tag:V5

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

nej^KIX.UAS

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

nej^{dsRNA.UAS.cKa}

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Raj and Sarkar, 2019)

nej^EP950

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN7^{102Q.T.UAS.Tag:NLS(SV40-largeT)}

(Latouche et al., 2007)

nej^ΔBHQ.UAS

exacerbates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Raj and Sarkar, 2019)

nej^ΔHQ.UAS

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

nej^hs.PA

model of alcohol use disorder

is ameliorated by slo^MI02233

(Ghezzi et al., 2017)

nej^ΔNZK.UAS

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

nej^ΔQ.UAS

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

nej^P

exacerbates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Taylor et al., 2003)

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Jung and Bonini, 2007)

modeled by Hsap\ATXN3^tr.Q46.UAS

(Jung and Bonini, 2007)

nej^EP1410

ameliorates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Huen and Chan, 2005)

nej^EP1149

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Taylor et al., 2003)

nej^EP1179

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Taylor et al., 2003)

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Jung and Bonini, 2007)

modeled by Hsap\ATXN3^tr.Q46.UAS

(Jung and Bonini, 2007)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CREBBP; CREB binding protein

12 of 15

600140

Rubinstein-Taybi syndrome

EP300; E1A binding protein p300

12 of 15

602700

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

nej

enzymatic study, western blot

(Tie et al., 2016)

nej - CG14451

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

nej - Clk

anti bait coimmunoprecipitation, autoradiography

(Hung et al., 2007)

nej - EcR

anti tag coimmunoprecipitation, anti tag western blot

(Kirilly et al., 2011)

nej - HDAC1

anti bait coimmunoprecipitation, western blot

(Tie et al., 2016)

nej - His3

histone acetylase assay, western blot

(Tie et al., 2012)

nej - HnRNP-K

anti bait coimmunoprecipitation, western blot

(Mallik and Lakhotia, 2010)

nej - Hrb87F

anti bait coimmunoprecipitation, western blot

(Mallik and Lakhotia, 2010)

nej - Jra

enzymatic study, western blot

(Zhang et al., 2013)

nej - Lpt

anti tag coimmunoprecipitation, western blot

(Zraly et al., 2020)

nej - MEP-1

anti bait coimmunoprecipitation, Identification by mass spectrometry

(Reddy et al., 2010)

nej - Mad

pull down, autoradiography

(Lilja et al., 2007)

nej - Mef2

two hybrid

(Lin and Baines, 2019)

nej - Mi-2

anti bait coimmunoprecipitation, Identification by mass spectrometry

(Reddy et al., 2010)

nej - Pc

anti bait coimmunoprecipitation, western blot, enzymatic study, pull down

(Tie et al., 2016)

nej - Polr2A

anti bait coimmunoprecipitation, western blot

(Tie et al., 2016)

nej - Psc

anti bait coimmunoprecipitation, western blot

(Tie et al., 2016)

nej - Sbf

ion exchange chromatography, molecular sieving, peptide massfingerprinting, western blot

(Petruk et al., 2001)

nej - Sirt1

anti bait coimmunoprecipitation, western blot, pull down, autoradiography

(Smolik, 2009)

nej - TfIIB

anti bait coimmunoprecipitation, anti tag western blot

(Boija et al., 2017)

nej - Utx

pull down, western blot, anti bait coimmunoprecipitation, molecular sieving, anti tag western blot

(Tie et al., 2012)

nej - arm

pull down, western blot, anti bait coimmunoprecipitation, autoradiography, anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2007)

nej - asf1

anti bait coimmunoprecipitation, western blot

(Das et al., 2009)

nej - bcd

anti tag coimmunoprecipitation, anti tag western blot, pull down, autoradiography

(Fu et al., 2004)

nej - brm

anti bait coimmunoprecipitation, western blot, molecular sieving, pull down, anti tag western blot

(Tie et al., 2012)

nej - ci

anti tag coimmunoprecipitation, anti tag western blot, pull down, molecular weight estimation by staining, two hybrid, lambda phage display

(Han et al., 2019, Han et al., 2015, Chen et al., 2000, Akimaru et al., 1997)

nej - dl

anti bait coimmunoprecipitation, western blot

(Holmqvist et al., 2012)

nej - en

anti tag coimmunoprecipitation, anti tag western blot

(Luo et al., 2017)

nej - gcm

anti bait coimmunoprecipitation, anti tag western blot

(Flici et al., 2014)

nej - mei-41

anti tag coimmunoprecipitation, anti tag western blot

(Smolik and Jones, 2007)

nej - repo

anti bait coimmunoprecipitation, western blot

(Flici et al., 2014)

nej - trr

anti bait coimmunoprecipitation, western blot

(Tie et al., 2014)

nej - trx

anti bait coimmunoprecipitation, western blot, pull down, ion exchange chromatography, molecular sieving, peptide massfingerprinting, anti tag western blot

(Tie et al., 2014, Petruk et al., 2001)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

nej

suppressible

arm

(Waltzer and Bienz, 1998)

nej

suppressible

ash1

(Bantignies et al., 2000)

nej

suppressible

cgd

(Anderson et al., 2005)

nej

enhanceable

cgd

(Anderson et al., 2005)

nej

suppressible

CrebA

(Anderson et al., 2005)

nej

enhanceable

CrebA

(Anderson et al., 2005)

nej

suppressible

CrebB

(Anderson et al., 2005)

nej

enhanceable

dpp

(Anderson et al., 2005)

suppressible

(Tie et al., 2009)

enhanceable

(Anderson et al., 2005)

nej

suppressible

enc

(Anderson et al., 2005)

nej

suppressible

(Anderson et al., 2005)

nej

enhanceable

eya

(Anderson et al., 2005)

nej

suppressible

fru

(Sethi et al., 2019)

nej

suppressible

(Anderson et al., 2005)

nej

suppressible

Glo1

(Anderson et al., 2005)

nej

enhanceable

kni

(Anderson et al., 2005)

nej

enhanceable

(Anderson et al., 2005)

nej

suppressible

l(3)71DEc

(Anderson et al., 2005)

nej

suppressible

l(3)72Df

(Anderson et al., 2005)

nej

suppressible

l(3)L3659

(Anderson et al., 2005)

nej

suppressible

lilli

(Anderson et al., 2005)

nej

enhanceable

lncRNA:Hsrω

(Mallik and Lakhotia, 2010)

nej

suppressible

lncRNA:Hsrω

(Mallik and Lakhotia, 2010)

nej

suppressible

luc

(Anderson et al., 2005)

nej

suppressible

Nrg

(Zhang et al., 2014)

nej

enhanceable

Pfas

(Anderson et al., 2005)

nej

enhanceable

Phm

(Anderson et al., 2005)

nej

suppressible

Pka-R1

(Bantignies et al., 2000)

nej

enhanceable

rho

(Anderson et al., 2005)

nej

suppressible

RhoGAP93B

(Anderson et al., 2005)

nej

suppressible

(Anderson et al., 2005)

nej

enhanceable

Scr

(Anderson et al., 2005)

nej

enhanceable

sgg

(Waltzer and Bienz, 1998)

nej

enhanceable

sog

(Anderson et al., 2005)

nej

enhanceable

spi

(Anderson et al., 2005)

nej

suppressible

srp

(Anderson et al., 2005)

nej

suppressible

sty

(Anderson et al., 2005)

nej

suppressible

Tpl

(Anderson et al., 2005)

nej

suppressible

Tsp42Ea

(Anderson et al., 2005)

nej

enhanceable

Ubx

(Anderson et al., 2005)

nej

enhanceable

Xrp1

(Anderson et al., 2005)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

nej

(Florence and McGinnis, 1998, Akimaru et al., 1997)

enhanceable

nej

(Florence and McGinnis, 1998)

Dfd

enhanceable

nej

(Florence and McGinnis, 1998)

dpp

enhanceable

nej

(Waltzer and Bienz, 1999)

gcm

suppressible

nej

(Popkova et al., 2012)

enhanceable

nej

(Müller et al., 2005)

suppressible

nej

(Müller et al., 2005)

krz

enhanceable

nej

(Bantignies et al., 2002)

enhanceable

nej

(Singh et al., 2005)

Myb

enhanceable

nej

(Fung et al., 2003)

Myb

suppressible

nej

(Okada et al., 2002)

Nsf2

suppressible

nej

(Laviolette et al., 2005)

enhanceable

nej

(Tie et al., 2009)

qed

enhanceable

nej

(Florence, 1999.7.22)

sax

suppressible

nej

(Waltzer and Bienz, 1999)

shg

suppressible

nej

(Waltzer and Bienz, 1998)

suppressible

nej

(Kumar et al., 2004)

enhanceable

nej

(Kumar et al., 2004)

Ubx

enhanceable

nej

(Florence and McGinnis, 1998)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261617

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Negative Regulators of Wnt-TCF Signaling Pathway -: Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
Positive Regulators of Wnt-TCF Signaling Pathway -: Positive regulators of Wnt-TCF (canonical Wnt) signaling up-regulate the pathway, resulting in the enhancement of transcriptional regulation mediated by β-catenin (arm).
Positive Regulators of Hedgehog Signaling Pathway -: Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-29

Cytogenetic map

8F7-8F9

Sequence location

X:9,665,442..9,687,066 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

8F7-8F9

Limits computationally determined from genome sequence between P{EP}EP912^EP912 and P{EP}nej^EP1149&P{EP}nej^EP1179

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

8F4-8F8

(determined by in situ hybridisation)

(Peter et al., 2002)

8F4-8F7

(determined by in situ hybridisation)

(Schäfer et al., 1999.6.15, Schäfer et al., 1999.12.2)

8F-9A

(Chen, 1997.2.5)

8F6-8F7

(determined by in situ hybridisation)

(Schäfer et al., 1999.8.12)

8F-9A

(determined by in situ hybridisation)

(Akimaru et al., 1997)

Experimentally Determined Recombination Data

Location

1-29

(FlyBase, 2016)

1-30.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (29)

Bloomington

3729

w^* nej³/FM7c

32573

w^*; P{UAS-nej.wt-V5}3

34040

w^* nej^0.3/FM7c

3730

w^*; P{hs-nej⁺}1

32576

P{UAS-nej.siRNA}hp12, w^*

32574

w^*; P{UAS-nej.F2161A-V5}2

30733

107202

v102885

v105115

v46534

w¹¹¹⁸; P{GD8975}v46534

v46535

w¹¹¹⁸; P{GD8975}v46535/TM3

More stocks available...

Genomic Clones (23)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (28)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261617

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261617

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43856

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Takakura et al., 2021, Mazina et al., 2018, Doiguchi et al., 2016, Tie et al., 2014, Holmqvist et al., 2012, Kirilly et al., 2011, Zhao et al., 2009, Lilja et al., 2003, Petruk et al., 2001)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: nej CG15319

(Bayraktaroglu, 2000.4.12)

Source for merge of: nej l(1)G0350 l(1)G0112 l(1)G0470 CG15321

(Yin et al., 2007)

Source for merge of: nej anon-WO03040301.89

(FlyBase, 1996-)

Additional comments

Annotations CG15321 eliminated in release 5.25 of the genome annotation.

(FlyBase Genome Annotators, 2010)

FlyBase curator comment: "EP1410" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise.

(Müller et al., 2005)

FlyBase curator comment: "EP950" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise. FlyBase curator comment: "EP1410" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise.

(Singh et al., 2005)

FlyBase curator comment: "EP950" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise.

(Pena-Rangel et al., 2002)

"l(1)G0464" may affect "nej".

(Peter et al., 2002)

"l(1)G0350" may affect "CG15321".

(Peter et al., 2002)

"l(1)G0112" may affect "CG15321".

(Peter et al., 2002)

"l(1)G0470" may affect "CG15321".

(Peter et al., 2002)

"l(1)G0350" may affect "CG15321". "l(1)G0286" may affect "CG15321". "l(1)G0137" may affect "CG15321". "l(1)G0112" may affect "CG15321". "l(1)G0270" may affect "CG15321". "l(1)G0470" may affect "CG15321". "l(1)G0236" may affect "CG15321".

(Peter et al., 2002)

Source for merge of nej anon-WO03040301.89 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

Overexpression of nej can repress or activate wg signaling depending on the context.

(Li et al., 2007)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.

(Inlow and Restifo, 2004)

nej functions in eye specification. In addition, nej has a role in cell fate determination during eye development, in particular in R3/R4 cell fate choice and also probably in the specification of the R7 photoreceptor.

(Kumar et al., 2004)

nej and Myb work together to promote efficient progression through mitosis.

(Fung et al., 2003)

Reduction in maternally contributed nej protein levels in embryos leads to reductions in expression of tld, scw, tsg and rho, but not dpp, in early embryogenesis. rho expression is even lost in cells containing signal dependent phosphorylated Mad protein (a marker of BMP signaling).

(Lilja et al., 2003)

nej function is necessary for ci-mediated transactivation of wg during embryogenesis.

(Chen et al., 2000)

Postsynaptic nej is necessary for normal presynaptic functional development.

(Marek et al., 2000)

nej functions as a co-activator during dpp signalling. Mad may recruit nej to effect the transcriptional activation of dpp responsive genes during development.

(Waltzer and Bienz, 1999)

The nej gene product represses pan to antagonise wg signalling.

(Waltzer and Bienz, 1998)

nej is necessary for dl-mediated activation of the twi promoter.

(Akimaru et al., 1997)

Isolated from a genomic library using a fragment of the C.elegans CBP gene as a probe, under low stringency conditions.

(Akimaru et al., 1997)

nej has been cloned and characterised.

(Akimaru et al., 1997)

Using the co-transfection assay with the nej expression plasmid results demonstrate trans-activation by Myb and functional domains of Myb involved in this activity are identified.

(Hou et al., 1997)

Origin and Etymology

Discoverer

Etymology

'nejire' means 'twist' in Japanese.

(Akimaru et al., 1997)

Identification

External Crossreferences and Linkouts ( 89 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43856

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261617

DRscDB

43856/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0261617

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15319

FlyMine - An integrated database for Drosophila genomics

FBgn0261617

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43856

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG15319

MARRVEL_MODEL

43856

modMine - A data warehouse for the modENCODE project

FBgn0261617

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O01368

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261617

DRSC - Results frm RNAi screens

FBgn0261617

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (36)

Reported As

Symbol Synonym

CBP

(Mazina et al., 2021, Takakura et al., 2021, Duffraisse et al., 2020, Nakamura et al., 2020, Zraly et al., 2020, Gutierrez-Perez et al., 2019, Haberle et al., 2019, Han et al., 2019, Raj and Sarkar, 2019, Boija et al., 2017, Chung et al., 2017, Ghezzi et al., 2017, Song et al., 2017, Doiguchi et al., 2016, Flegel et al., 2016, Hirano et al., 2016, Lee et al., 2016, Tie et al., 2016, Baëza et al., 2015, Cutler et al., 2015, Dietz et al., 2015, Ho et al., 2014, Singh et al., 2014, Tie et al., 2014, Zhang et al., 2014, Darbo et al., 2013, Gisselbrecht et al., 2013, Hirano et al., 2013, Zhang et al., 2013, Bodai et al., 2012, Holmqvist et al., 2012, Kellner et al., 2012, Tie et al., 2012, Kirilly et al., 2011, Nègre et al., 2011, Pruteanu-Malinici et al., 2011, Schuettengruber et al., 2011, Boyles et al., 2010, Herz et al., 2010, Mallik and Lakhotia, 2010, Reddy et al., 2010, Das et al., 2009, Smolik, 2009, Tie et al., 2009, Fanti et al., 2008, Parker et al., 2008, Tavantzis and Warrick, 2008, Wang et al., 2008, Fortini, 2007, Hung et al., 2007, Latouche et al., 2007, Li et al., 2007, Lim et al., 2007, Parker et al., 2007, Yin et al., 2007, Parker et al., 2006, Anderson et al., 2005, Eissenberg et al., 2005, Fernandez-Funez et al., 2005, Huen and Chan, 2005, Ma, 2005, Kumar et al., 2004, Lipsick, 2004, Ogden et al., 2004, Sanchez-Elsner and Sauer, 2004, Smith et al., 2004, Yang, 2004, Fernandez-Funez et al., 2003, Petruk et al., 2003, Ingham and McMahon, 2001, Sharpe et al., 2001, Attisano and Wrana, 2000, Chen and Courey, 2000, Glass and Rosenfeld, 2000, Goodman and Smolik, 2000, Kouzarides, 1999, Mannervik et al., 1999, Matise and Joyner, 1999, Murone et al., 1999, Novak and Dedhar, 1999, Anonymous, 1998, Ingham, 1998, Chen, 1997.2.5, Tabin and McMahon, 1997)

CBP/p300

(Mazina et al., 2019, Fei et al., 2015, Stadeli, 2006, Newfeld and Takaesu, 2002, Newfeld et al., 2002, Vo and Goodman, 2001, Behrens, 2000, Seidensticker and Behrens, 2000)

CBP_

(Bashirullah et al., 2006)

CG15319

(Hosono et al., 2015, Ni et al., 2011.7.19, Reddy et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Grieder et al., 2007, Laviolette et al., 2005, Ramet et al., 2002)

CG15321

(FlyBase Genome Annotators, 2010, Popodi et al., 2010-, Venken et al., 2010, Keleman et al., 2009.8.5, Ni et al., 2008, Stofanko et al., 2008, Grieder et al., 2007, Grieder et al., 2007, Yin et al., 2007, Manak et al., 2006)

Cbp

(Chen et al., 1998, Chen et al., 1996)

Crbp

Nej

(Wei et al., 2020, Kwon et al., 2018, Ramirez-Roman et al., 2018, Mbodj et al., 2013, van Eyk et al., 2011, Zhou et al., 2011, Anholt and Williams, 2010, Guenin et al., 2010, Martin et al., 2007, Yin et al., 2007)

Nejire

(Reddy et al., 2010)

P300

(Haberle et al., 2019)

anon-WO0147981.11

(Modjtahedi, 2001.7.5)

anon-WO03040301.89

cbp

(Maurer et al., 2016, Ayscough, 1998)

dCBP

(Martin et al., 2021, Sethi et al., 2019, Li et al., 2018, Han et al., 2015, Flici et al., 2014, Bodai et al., 2012, Holmqvist et al., 2012, Ito et al., 2012, Zhai et al., 2012, Crump et al., 2011, Boyles et al., 2010, Smolik, 2009, Zhao et al., 2009, Hong et al., 2008, Tavantzis and Warrick, 2008, Jung and Bonini, 2007, Lilja et al., 2007, Nagraj et al., 2007, Nibu et al., 2007, Schuettengruber et al., 2007, Smolik and Jones, 2007, Petruk et al., 2006, Fu and Ma, 2005, Fu et al., 2004, Kumar et al., 2004, Kwon et al., 2004, Li et al., 2004, Smith et al., 2004, Evans et al., 2003, Fung et al., 2003, Li et al., 2003, Lilja et al., 2003, Lilja et al., 2003, Maves and Schubiger, 2003, Taylor et al., 2003, Bantignies et al., 2002, Cavarec et al., 2002, Jia et al., 2002, Ludlam et al., 2002, Newman et al., 2002, Simon and Tamkun, 2002, Fry and Peterson, 2001, Ludlam et al., 2001, Petruk et al., 2001, Petruk et al., 2001, Bantignies et al., 2000, Chen et al., 2000, Davis et al., 2000, Ludlam et al., 2000, Marek et al., 2000, Aza-Blanc and Kornberg, 1999, Bantignies et al., 1999, Govind, 1999, Newman et al., 1999, Roose and Clevers, 1999, Vousden, 1999, Anonymous, 1998, Bantignies et al., 1998, Giles et al., 1998, Goodrich and Scott, 1998, Miska, 1998, Waltzer and Bienz, 1998, Akimaru et al., 1997, Akimaru et al., 1997, Florence and McGinnis, 1997, Hou et al., 1997)

dKAT3

(Crump et al., 2011)

dmCBP

(Pandey et al., 2002)

l(1)G0112

l(1)G0350

l(1)G0470

nej

(Martin et al., 2021, Aboukilila et al., 2020, Cho et al., 2020, Graves et al., 2020, Hung et al., 2020, Jeon et al., 2020, Park et al., 2020, Zhao et al., 2020, Nakamura et al., 2019, Sang Cho et al., 2019, Rust et al., 2018, Salazar and Yamamoto, 2018, Ghezzi et al., 2017, Janssens et al., 2017, Transgenic RNAi Project members, 2017-, Vallejos Baier et al., 2017, Brewer-Jensen et al., 2016, Malartre, 2016, Maurer et al., 2016, Morimoto et al., 2016, Moulton and Letsou, 2016, Schwartz et al., 2016, Yu et al., 2016, Jia et al., 2015, Lee et al., 2015, Ugrankar et al., 2015, Vedanayagam and Garrigan, 2015, Flici et al., 2014, Rogers et al., 2014, Yan et al., 2014, Czech et al., 2013, de Celis et al., 2013.9.11, Ghezzi et al., 2013, Neumüller et al., 2013, Tögel et al., 2013, Vaque et al., 2013, Yamamoto et al., 2013-, Bodai et al., 2012, Busser et al., 2012, Hainaut et al., 2012, Hilgers et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Legent et al., 2012, Popkova et al., 2012, Zhai et al., 2012, Hilgers et al., 2011, Kirilly et al., 2011, Smolik, 2011.2.28, Toku et al., 2011, He et al., 2010, Pospisilik et al., 2010, Sela et al., 2010, Smolik, 2010.11.15, Stofanko et al., 2010, Ayroles et al., 2009, Dworkin et al., 2009, Stofanko et al., 2008, Straub et al., 2008, Gilfillan et al., 2007, Grieder et al., 2007, Grieder et al., 2007, Hung et al., 2007, Li et al., 2007, Lilja et al., 2007, Nagraj et al., 2007, Smolik and Jones, 2007, Weber et al., 2007, Friedman and Perrimon, 2006, DasGupta et al., 2005, Huen and Chan, 2005, Laviolette et al., 2005, Ito et al., 2004, Stanyon et al., 2004, Takeyama et al., 2004, Petruk et al., 2001)

nej CBP

(Singh et al., 2005)

nejire

(Shimaji et al., 2019)

p300

(Su et al., 2019, Fei et al., 2015, Vaqué et al., 2013, Nègre et al., 2011, Lee and Stallcup, 2006)

p300/CBP

(Haberle et al., 2019, Yeung et al., 2011, Lee and Stallcup, 2006, Mizzen and Allis, 2000)

Name Synonyms

CBP/Nejire

(Yin et al., 2007)

CREB Binding Protein

(Cutler et al., 2015)

CREB binding protein

(Gisselbrecht et al., 2013, Kellner et al., 2012, Nègre et al., 2011, Zhao et al., 2009, Hart and Warrick, 2008, Tavantzis and Warrick, 2008, Yin et al., 2007, Park et al., 2001, Ingham, 1998)

CREB-binding protein

(Tie et al., 2016, Kirilly et al., 2011, Boyles et al., 2010, Smolik, 2009, Parker et al., 2008, Fortini, 2007, Johnston and Scott, 1998, Chen et al., 1996)

Creb binding protein

(Flici et al., 2014)

Cyclic AMP Response Element Binding protein

(Smolik, 2009)

Drosophila CREB-binding

(Kwon et al., 2004)

Drosophila CREB-binding protein

(Nibu et al., 2007)

Nejire

(Chetverina et al., 2020, Schmidt et al., 2011, Das et al., 2009, Martin et al., 2007, Yin et al., 2007)

lethal (1) G0112

lethal (1) G0350

lethal (1) G0470

protein

(Kwon et al., 2004)

Secondary FlyBase IDs

FBgn0015624
FBgn0030149
FBgn0066809
FBgn0028319
FBgn0027307
FBgn0040152
FBgn0030150

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

modENCODE_regulation_chromatin

genome binding

Genome-wide localization of chromatin factors by ChIP-chip and ChIP-Seq.

References (355)

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