FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\nej
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General Information
Symbol
Dmel\nej
Species
D. melanogaster
Name
nejire
Annotation Symbol
CG15319
Feature Type
protein_coding_gene
FlyBase ID
FBgn0261617
Gene Model Status
Current
Stock Availability
34 publicly available
Gene Summary
nejire (nej) encodes the transcriptional co-activator CBP. It acetylates several nuclear proteins, including the histone encoded by His3 on K18, K27, and H4 on K8. By regulating gene expression, the product of nej has roles in cell proliferation, cell signaling and differentiation, and in developmental patterning. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

CBP, dCBP, CREB-binding protein, CREB binding protein, CBP/p300

Key Links
Genomic Location
Cytogenetic map
8F7-8F9
Sequence location
Recombination map
1-29
RefSeq locus
NC_004354 REGION:9665442..9687066
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (39 terms)
Molecular Function (12 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
enables DNA-binding transcription factor binding
inferred from physical interaction with FLYBASE:ci; FB:FBgn0004859
(Akimaru et al., 1997)
enables histone acetyltransferase activity
inferred from direct assay
(Petruk et al., 2003, Ludlam et al., 2002)
enables histone H3K18 acetyltransferase activity
inferred from direct assay
(Tie et al., 2009)
enables histone H3K27 acetyltransferase activity
inferred from direct assay
(Tie et al., 2012, Tie et al., 2009)
enables histone methyltransferase binding
inferred from physical interaction with FLYBASE:ash1; FB:FBgn0005386
(Bantignies et al., 2000)
enables protein binding
inferred from physical interaction with FLYBASE:mod; FB:FBgn0002780
(Bantignies et al., 2002)
inferred from physical interaction with FLYBASE:Clk; FB:FBgn0023076
(Hung et al., 2007)
enables RNA polymerase II-specific DNA-binding transcription factor binding
inferred from physical interaction with UniProtKB:P19538
(Han et al., 2015)
enables transcription coactivator activity
inferred from genetic interaction with FLYBASE:ci; FB:FBgn0004859
(Chen et al., 2000)
inferred from direct assay
(Hung et al., 2007)
inferred from genetic interaction with FLYBASE:per; FB:FBgn0003068
(Hung et al., 2007)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
enables cAMP response element binding protein binding
traceable author statement
(Marek et al., 2000)
enables chromatin DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000351795
(GO Reference Genome Project, 2011-)
enables histone acetyltransferase activity
inferred from electronic annotation with InterPro:IPR013178, InterPro:IPR031162
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000351795
(GO Reference Genome Project, 2011-)
enables transcription coactivator activity
inferred from biological aspect of ancestor with PANTHER:PTN000351795
(GO Reference Genome Project, 2011-)
enables transcription coregulator activity
inferred from electronic annotation with InterPro:IPR003101, InterPro:IPR036529
(InterPro Project Members, 2004-)
enables zinc ion binding
inferred from electronic annotation with InterPro:IPR000433
(InterPro Project Members, 2004-)
Biological Process (20 terms)
Terms Based on Experimental Evidence (18 terms)
CV Term
Evidence
References
involved_in chromatin organization
inferred from mutant phenotype
(Ludlam et al., 2002)
involved_in circadian regulation of gene expression
inferred from mutant phenotype
(Hung et al., 2007)
involved_in compound eye development
inferred from mutant phenotype
(Mallik and Lakhotia, 2010)
involved_in compound eye morphogenesis
inferred from mutant phenotype
(Kumar et al., 2004)
involved_in DNA replication checkpoint signaling
inferred from mutant phenotype
(Smolik and Jones, 2007)
involved_in follicle cell of egg chamber development
inferred from mutant phenotype
(Jia et al., 2015)
involved_in glial cell migration
inferred from mutant phenotype
(Schmidt et al., 2011)
involved_in locomotor rhythm
inferred from mutant phenotype
(Hung et al., 2007)
involved_in negative regulation of canonical Wnt signaling pathway
inferred from mutant phenotype
(Li et al., 2007)
involved_in neurotransmitter secretion
inferred from mutant phenotype
(Marek et al., 2000)
involved_in positive regulation of canonical Wnt signaling pathway
inferred from mutant phenotype
(Li et al., 2007)
involved_in positive regulation of smoothened signaling pathway
inferred from genetic interaction with FLYBASE:ci; FB:FBgn0004859
(Akimaru et al., 1997)
inferred from mutant phenotype
(Chen et al., 2000)
involved_in positive regulation of transcription by RNA polymerase II
inferred from mutant phenotype
(Chen et al., 2000)
inferred from genetic interaction with FLYBASE:ci; FB:FBgn0004859
(Chen et al., 2000)
involved_in R3/R4 cell fate commitment
inferred from mutant phenotype
(Kumar et al., 2004)
involved_in R7 cell differentiation
inferred from mutant phenotype
(Kumar et al., 2004)
involved_in regulation of mitotic nuclear division
inferred from mutant phenotype
(Fung et al., 2003)
inferred from genetic interaction with FLYBASE:Myb; FB:FBgn0002914
(Fung et al., 2003)
involved_in synapse assembly
inferred from mutant phenotype
(Marek et al., 2000)
involved_in thermosensory behavior
inferred from mutant phenotype
(Hong et al., 2008)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in hemopoiesis
traceable author statement
(Evans et al., 2003)
involved_in positive regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN000351795
(GO Reference Genome Project, 2011-)
involved_in regulation of DNA-templated transcription
inferred from electronic annotation with InterPro:IPR003101, InterPro:IPR013178, InterPro:IPR036529
(InterPro Project Members, 2004-)
Cellular Component (7 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
part_of histone acetyltransferase complex
inferred from direct assay
(Petruk et al., 2003)
part_of histone methyltransferase complex
inferred from direct assay
(Petruk et al., 2003)
located_in nucleoplasm
inferred from high throughput direct assay
(Lye et al., 2014)
located_in nucleus
inferred from direct assay
(Bantignies et al., 2002, Marek et al., 2000)
located_in polytene chromosome
inferred from direct assay
(Petruk et al., 2001, Bantignies et al., 2000)
part_of protein-containing complex
inferred from physical interaction with FLYBASE:mod; FB:FBgn0002780
(Bantignies et al., 2002)
inferred from physical interaction with FLYBASE:ash1; FB:FBgn0005386
(Bantignies et al., 2000)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of histone acetyltransferase complex
inferred from biological aspect of ancestor with PANTHER:PTN000351795
(GO Reference Genome Project, 2011-)
part_of transcription regulator complex
inferred from biological aspect of ancestor with PANTHER:PTN000351795
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
histone acetyltransferase activity
RHEA 21992:
Summaries
Gene Snapshot
nejire (nej) encodes the transcriptional co-activator CBP. It acetylates several nuclear proteins, including the histone encoded by His3 on K18, K27, and H4 on K8. By regulating gene expression, the product of nej has roles in cell proliferation, cell signaling and differentiation, and in developmental patterning. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
TRITHORAX ACETYLATION COMPLEX 1 -
The Trithorax Acetylation Complex 1 (TAC1) possesses histone H3 K4 methyltransferase and histone acetyltransferase activities. (Adapted from FBrf0192625 and FBrf0167942).
HISTONE ACETYLTRANSFERASES -
Histone acetyltransferases catalyze the acetylation of conserved lysine residues on histone proteins by transferring an acetyl group from acetyl CoA to form ε-N-acetyl lysine. (Adapted from PMID:11395403).
Pathway (FlyBase)
NEGATIVE REGULATORS OF WNT-TCF SIGNALING PATHWAY -
Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
POSITIVE REGULATORS OF WNT-TCF SIGNALING PATHWAY -
Positive regulators of Wnt-TCF (canonical Wnt) signaling up-regulate the pathway, resulting in the enhancement of transcriptional regulation mediated by β-catenin (arm).
POSITIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY -
Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.
Summary (Interactive Fly)

CBP, a transcriptional co-activator that interacts with a large number of developmentally important transcription factors - acetylates several nuclear proteins, including histone H3 on K18, K27, and H4 on K8. - roles in cell proliferation, cell signaling and differentiation, and in developmental patterning

Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\nej for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry M9MS40)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Stage-specific extension of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated.

Gene model reviewed during 5.43

Gene model reviewed during 5.42

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.52

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
nej-RB
FBtr0071402
NM_079903
11733
3276
nej-RC
FBtr0302722
NM_001201646
11704
3282
nej-RD
FBtr0302723
NM_001201647
15729
3276
nej-RE
FBtr0333521
NM_001272458
15400
3266
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
nej-PB
FBpp0071337
340.7
3276
8.86
Q9W321
NP_524642
AAF46516
nej-PC
FBpp0291862
341.4
3282
8.89
M9MS40
NP_001188575
ADV37657
nej-PD
FBpp0291863
340.7
3276
8.86
Q9W321
NP_001188576
ADV37658
nej-PE
FBpp0305701
339.7
3266
8.86
M9PH39
NP_001259387
AGB95230
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

3276 aa isoforms: nej-PB, nej-PD
Additional Polypeptide Data and Comments
Reported size (kDa)

3190 (aa); 332 (kD)

(Akimaru et al., 1997)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nej using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.95

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 9 -- 17
organism

Comment: extended 3' UTR isoform

(Hilgers et al., 2011)
embryonic stage 12 -- 15
larval nervous system

Comment: extended 3' UTR isoform

(Hilgers et al., 2011)
Additional Descriptive Data

Zygotic-specific isoforms of nej with long 3' UTR extensions were observed. The 3' UTR extension isoforms are highly enriched in nervous system tissues.

(Hilgers et al., 2011)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4 -- 12
organism | ubiquitous
(Ludlam et al., 2002)
embryonic stage 5
• nucleus
(Lilja et al., 2003)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of histone acetyltransferase complex
inferred from direct assay
(Petruk et al., 2003)
part_of histone methyltransferase complex
inferred from direct assay
(Petruk et al., 2003)
located_in nucleoplasm
inferred from high throughput direct assay
(Lye et al., 2014)
located_in nucleus
inferred from direct assay
(Bantignies et al., 2002, Marek et al., 2000)
located_in polytene chromosome
inferred from direct assay
(Petruk et al., 2001, Bantignies et al., 2000)
part_of protein-containing complex
inferred from physical interaction with FLYBASE:mod; FB:FBgn0002780
(Bantignies et al., 2002)
inferred from physical interaction with FLYBASE:ash1; FB:FBgn0005386
(Bantignies et al., 2000)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\nej in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 42 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 34 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of nej
Transgenic constructs containing regulatory region of nej
Aberrations (Deficiencies and Duplications) ( 10 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal, with Scer\GAL4en-e16E
lethal, with Scer\GAL4hs.2sev
lethal, with Scer\GAL4insc-Mz1407
lethal, with Scer\GAL4pnr-MD237
lethal (with nej3)
lethal (with nejP)
lethal (with nejQ7)
lethal (with nejS103)
lethal (with nejS342)
lethal (with nejTA57)
lethal | adult stage
lethal | pharate adult stage
lethal | pharate adult stage, with Scer\GAL430A
lethal | pupal stage, with Scer\GAL4Act5C.PI
lethal | recessive
lethal - all die before end of larval stage, with Scer\GAL4elav.PLu
lethal - all die before end of prepupal stage
lethal - all die before end of prepupal stage, with Scer\GAL4GMR.PF
lethal - all die before end of P-stage
lethal - all die before end of pupal stage, with Scer\GAL4GMR.PF
lethal - all die before end of pupal stage, with Scer\GAL4pnr-MD237
lethal - all die during embryonic stage | recessive
lethal - all die during larval stage, with Scer\GAL4Mef2.PR
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
partially lethal, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
partially lethal - majority die, with Scer\GAL4hs.2sev
partially lethal - majority die, with Scer\GAL4OK363
partially lethal - majority die | embryonic stage, with Scer\GAL4Act5C.PI
partially lethal - majority die | larval stage, with Scer\GAL4Act5C.PI
partially lethal - majority die | pharate adult stage, with Scer\GAL430A
partially lethal - majority die | recessive
partially lethal - majority live
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female fertile, with Scer\GAL4nanos.PG
male sterile
Other Phenotypes
Allele
abnormal cell migration
abnormal courtship behavior | conditional, with Scer\GAL4Or47b.7.467
abnormal developmental rate | recessive
abnormal eye color, with Scer\GAL4GMR.PF
abnormal locomotor behavior | adult stage | heat sensitive, with Scer\GAL4hs.PU
abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4hs.PB
abnormal neuroanatomy
abnormal neuroanatomy, with Scer\GAL4elav.PLu
abnormal neuroanatomy | larval stage, with Scer\GAL4insc-Mz1407
abnormal neuroanatomy | larval stage, with Scer\GAL4ppk.PG
abnormal neuroanatomy | P-stage, with Scer\GAL4ppk.PU
abnormal neuroanatomy | third instar larval stage, with Scer\GAL4GMR.PU
abnormal neuroanatomy | third instar larval stage, with Scer\GAL4ppk.PU
abnormal neurophysiology
abnormal neurophysiology, with Scer\GAL4elav.PLu
abnormal neurophysiology, with Scer\GAL4Mhc.PW
abnormal neurophysiology | adult stage | conditional, with Scer\GAL4Or47b.7.467
abnormal pain response | adult stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
abnormal size | adult stage, with Scer\GAL4GMR.PU
chemical sensitive | dominant
decreased rate of movement | recessive
some die during embryonic stage | recessive
some die during larval stage, with Scer\GAL4elav.PLu
some die during pharate adult stage, with Scer\GAL4OK363
some die during prepupal stage | recessive
some die during prepupal stage | recessive, with Scer\GAL4GMR.PF
some die during P-stage
some die during pupal stage, with Scer\GAL4GMR.PF
some die during pupal stage, with Scer\GAL4pnr-MD237
visible, with Scer\GAL430A
visible, with Scer\GAL4bbg-C96
visible, with Scer\GAL4ey.PH
visible, with Scer\GAL4GMR.PF
visible, with Scer\GAL4GMR.PF, nej3
visible, with Scer\GAL4GMR.PF, nejΔBHQ.UAS
visible, with Scer\GAL4GMR.PF, nejΔNZK.UAS
visible, with Scer\GAL4GMR.PU
visible, with Scer\GAL4hs.2sev
visible, with Scer\GAL4hs.PB
visible, with Scer\GAL4OK363
visible, with Scer\GAL4OK386
visible, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4sca-537.4
visible (with nej131)
visible (with nejP)
visible (with nejTC41)
visible | adult stage, with Scer\GAL4en.PU
visible | adult stage, with Scer\GAL4ey.PH
visible | adult stage, with Scer\GAL4GMR.PF
visible | adult stage, with Scer\GAL4nub.PK
visible | recessive | somatic clone
visible | somatic clone
wild-type
Phenotype manifest in
Allele
abdominal 4 ventral denticle belt
abdominal 5 ventral denticle belt
adult abdominal segment 4 | ectopic, with Scer\GAL4hs.PB
adult abdominal segment 5, with Scer\GAL4hs.PB
adult olfactory receptor neuron Or47b | adult stage | conditional, with Scer\GAL4Or47b.7.467
amnioserosa | germline clone
axon | third instar larval stage, with Scer\GAL4GMR.PU
cephalopharyngeal skeleton
condensed chromosome | third instar larval stage, with Scer\GAL4hs.PB
cone cell | ectopic, with Scer\GAL4GMR.PF
cuticle
dendrite | larval stage, with Scer\GAL4ppk.PG
dendritic tree | P-stage, with Scer\GAL4ppk.PU
dorsocentral bristle, with Scer\GAL4sca-537.4
ectoderm | dorsal | germline clone
embryonic/first instar larval cuticle
embryonic/larval fat body | third instar larval stage, with Scer\GAL4SPARC-MI00329-GAL4
embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4Pxn.PS
embryonic/larval midgut | embryonic stage
embryonic/larval midgut | embryonic stage | germline clone
embryonic/larval proventriculus | embryonic stage
embryonic/larval salivary gland
embryonic/larval salivary gland, with Scer\GAL4SG
embryonic/larval tracheal dorsal trunk
embryonic/larval ventral tracheal anastomosis
embryonic antennal sense organ
embryonic head
embryonic midgut constriction 1
epidermis
extracellular matrix | third instar larval stage, with Scer\GAL4SPARC-MI00329-GAL4
eye, with Scer\GAL4ey.PH
eye, with Scer\GAL4GMR.PF
eye, with Scer\GAL4GMR.PF, nej3
eye, with Scer\GAL4GMR.PF, nejΔBHQ.UAS
eye, with Scer\GAL4GMR.PF, nejΔNZK.UAS
eye, with Scer\GAL4GMR.PU
eye, with Scer\GAL4hs.2sev
eye (with nej131)
eye (with nejP)
eye (with nejTC41)
eye | dorsal, with Scer\GAL4ey.PH
eye | somatic clone
eye disc, with Scer\GAL4ey.PH
eye disc, with Scer\GAL4GMR.PF
eye disc | somatic clone | third instar larval stage
eye disc | third instar larval stage, with Scer\GAL4hs.PB
eye photoreceptor cell, with Scer\GAL4GMR.PF
eye photoreceptor cell, with Scer\GAL4hs.2sev
eye photoreceptor cell (with nej131)
eye photoreceptor cell (with nejP)
eye photoreceptor cell | ectopic, with Scer\GAL4GMR.PF
eye photoreceptor cell | somatic clone
eye photoreceptor cell | third instar larval stage, with Scer\GAL4GMR.PU
filzkorper
ganglion mother cell | larval stage, with Scer\GAL4insc-Mz1407
gastric caecum
head
interommatidial bristle, with Scer\GAL4GMR.PF
interommatidial bristle, with Scer\GAL4hs.2sev
lamellocyte | third instar larval stage, with Scer\GAL4Pxn.PS
larval dorsal multidendritic neuron ddaC | P-stage, with Scer\GAL4ppk.PU
larval multidendritic class IV neuron, with Scer\GAL4ppk.PG
larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4ppk.PU
larval tracheal system
macrochaeta
median tooth
mouth hook
mystery cell, with Scer\GAL4GMR.PF
neuromuscular junction
neuromuscular junction, with Scer\GAL4elav.PLu
neuromuscular junction, with Scer\GAL4Mhc.PW
neuromuscular junction, with Scer\GAL4Mhc.PW, Scer\GAL4elav.PLu
neuromuscular junction & bouton
neuromuscular junction & bouton, with Scer\GAL4elav.PLu
neuromuscular junction & t-bar
ommatidial precursor cluster, with Scer\GAL4GMR.PF
ommatidium, with Scer\GAL4ey.PH
ommatidium, with Scer\GAL4GMR.PF
ommatidium, with Scer\GAL4hs.2sev
ommatidium (with nej131)
ommatidium (with nejP)
ommatidium (with nejTC41)
ommatidium | somatic clone
outer photoreceptor cell | ectopic, with Scer\GAL4hs.2sev
photoreceptor cell R3, with Scer\GAL4hs.2sev
photoreceptor cell R3 | ectopic, with Scer\GAL4hs.2sev
photoreceptor cell R4, with Scer\GAL4hs.2sev
photoreceptor cell R7, with Scer\GAL4hs.2sev
photoreceptor cell R7 | ectopic, with Scer\GAL4hs.2sev
pigment cell | somatic clone
retina, with Scer\GAL4GMR.PF
rhabdomere, with Scer\GAL4GMR.PF
scutellar bristle, with Scer\GAL4pnr-MD237
scutellar bristle, with Scer\GAL4sca-537.4
subperineurial glial cell
terminal organ | embryonic stage
thorax
tormogen cell, with Scer\GAL4sca-537.4
trichogen cell, with Scer\GAL4sca-537.4
ventral denticle belt | anterior
wing, with Scer\GAL430A
wing, with Scer\GAL4bbg-C96
wing, with Scer\GAL4OK363
wing, with Scer\GAL4OK386
wing blade, with Scer\GAL4bbg-C96
wing blade, with Scer\GAL4en.PU
wing blade, with Scer\GAL4nub.PK
wing margin, with Scer\GAL4bbg-C96
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (21)
Hsap\EP300
12 of 14
Yes
Yes
Hsap\CREBBP
11 of 14
No
Yes
Hsap\BAZ1B
2 of 14
No
No
Hsap\BRD4
2 of 14
No
No
1  
Hsap\BRDT
2 of 14
No
No
Hsap\BAZ1A
1 of 14
No
No
Hsap\BAZ2A
1 of 14
No
No
Hsap\BAZ2B
1 of 14
No
No
Hsap\BPTF
1 of 14
No
No
Hsap\BRD2
1 of 14
No
No
2  
Hsap\BRD3
1 of 14
No
No
Hsap\BRD8
1 of 14
No
No
Hsap\BRWD3
1 of 14
No
No
Hsap\CECR2
1 of 14
No
No
Hsap\HERC2
1 of 14
No
No
Hsap\INAFM1
1 of 14
No
No
Hsap\KAT2A
1 of 14
No
No
1  
Hsap\KAT2B
1 of 14
No
No
1  
Hsap\MDN1
1 of 14
No
No
Hsap\PBRM1
1 of 14
No
No
Hsap\TRIM33
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (18)
Rnor\Crebbp
12 of 14
Yes
Yes
Rnor\Ep300
3 of 14
No
Yes
Rnor\Baz1b
2 of 14
No
No
Rnor\Brd4
2 of 14
No
No
Rnor\Cecr2
2 of 14
No
No
Rnor\Ash1l
1 of 14
No
No
Rnor\Baz1a
1 of 14
No
No
Rnor\Brd2
1 of 14
No
No
Rnor\Brd3
1 of 14
No
No
Rnor\Brd8
1 of 14
No
No
Rnor\Brpf1
1 of 14
No
No
Rnor\Brwd1
1 of 14
No
No
Rnor\Brwd3
1 of 14
No
No
Rnor\Herc2
1 of 14
No
No
Rnor\Inafm1
1 of 14
No
No
Rnor\Kat2a
1 of 14
No
No
Rnor\Pbrm1
1 of 14
No
No
Rnor\Trim33
1 of 14
No
No
Mus musculus (laboratory mouse) (22)
Mmus\Crebbp
12 of 14
Yes
Yes
Mmus\Ep300
12 of 14
Yes
Yes
Mmus\Baz1b
2 of 14
No
Yes
Mmus\Brd2
2 of 14
No
No
Mmus\Brd4
2 of 14
No
No
Mmus\Cecr2
2 of 14
No
No
Mmus\Ash1l
1 of 14
No
No
Mmus\Baz2a
1 of 14
No
No
Mmus\Baz2b
1 of 14
No
No
Mmus\Bptf
1 of 14
No
No
Mmus\Brd3
1 of 14
No
No
Mmus\Brd8dc
1 of 14
No
No
Mmus\Brd8
1 of 14
No
No
Mmus\Brdt
1 of 14
No
No
Mmus\Brpf1
1 of 14
No
No
Mmus\Brwd1
1 of 14
No
No
Mmus\Herc2
1 of 14
No
No
Mmus\Inafm1
1 of 14
No
No
Mmus\Kat2a
1 of 14
No
No
Mmus\Kat2b
1 of 14
No
No
Mmus\Pbrm1
1 of 14
No
No
Mmus\Trim33
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (16)
Xtro\phf5a
10 of 13
Yes
Yes
Xtro\crebbp
9 of 13
No
Yes
Xtro\ep300
9 of 13
No
Yes
Xtro\atad2b
1 of 13
No
Yes
Xtro\baz1b
1 of 13
No
No
Xtro\baz2a
1 of 13
No
No
Xtro\brd2
1 of 13
No
No
Xtro\brpf1
1 of 13
No
No
Xtro\brwd3
1 of 13
No
No
Xtro\herc2
1 of 13
No
No
Xtro\kdm6a
1 of 13
No
No
Xtro\kri1
1 of 13
No
No
Xtro\myo9b
1 of 13
No
Yes
Xtro\rab25
1 of 13
No
No
Xtro\sqstm1
1 of 13
No
No
Xtro\tspan2
1 of 13
No
No
Danio rerio (Zebrafish) (21)
Drer\crebbpa
11 of 14
Yes
Yes
Drer\ep300b
11 of 14
Yes
Yes
Drer\crebbpb
10 of 14
No
Yes
Drer\ep300a
10 of 14
No
Yes
Drer\bptf
2 of 14
No
No
Drer\brd2a
2 of 14
No
No
Drer\brd8b
2 of 14
No
No
Drer\cecr2
2 of 14
No
No
Drer\baz1a
1 of 14
No
No
Drer\baz2ba
1 of 14
No
No
Drer\brd3a
1 of 14
No
No
Drer\brd4
1 of 14
No
No
Drer\brd8a
1 of 14
No
No
Drer\brdt
1 of 14
No
No
Drer\dtna
1 of 14
No
No
Drer\herc2
1 of 14
No
No
Drer\kat2b
1 of 14
No
No
Drer\LOC100001344
1 of 14
No
No
Drer\pbrm1
1 of 14
No
No
Drer\pbrm1l
1 of 14
No
No
Drer\trim24
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (22)
Cele\cbp-1
9 of 14
Yes
Yes
Cele\C29F9.5
4 of 14
No
Yes
Cele\C29F9.6
3 of 14
No
Yes
Cele\cbp-2
3 of 14
No
Yes
Cele\cbp-3
3 of 14
No
Yes
Cele\baz-2
2 of 14
No
No
Cele\C49F5.5
2 of 14
No
Yes
Cele\F13C5.2
2 of 14
No
No
Cele\pcaf-1
2 of 14
No
No
Cele\athp-2
1 of 14
No
No
Cele\bet-1
1 of 14
No
No
Cele\C06G3.6
1 of 14
No
No
Cele\dyb-1
1 of 14
No
No
Cele\F22F1.3
1 of 14
No
Yes
Cele\kcmf-1
1 of 14
No
No
Cele\sepa-1
1 of 14
No
Yes
Cele\sqst-1
1 of 14
No
No
Cele\sqst-3
1 of 14
No
No
Cele\sqst-4
1 of 14
No
No
Cele\sqst-5
1 of 14
No
No
Cele\Y32B12A.5
1 of 14
No
Yes
Cele\ZK1010.10
1 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (7)
Agam\AgaP_AGAP000029
9 of 12
Yes
Yes
Agam\AgaP_AGAP001099
2 of 12
No
No
Agam\AgaP_AGAP004434
2 of 12
No
No
Agam\AgaP_AGAP000668
1 of 12
No
No
Agam\AgaP_AGAP003845
1 of 12
No
No
Agam\AgaP_AGAP005187
1 of 12
No
No
Agam\AgaP_AGAP008017
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (28)
Atha\HAC5
9 of 13
Yes
Yes
Atha\HAC12
9 of 13
Yes
Yes
Atha\HAC1
8 of 13
No
Yes
Atha\HAC4
8 of 13
No
Yes
Atha\HAC2
6 of 13
No
Yes
Atha\AT1G16705
2 of 13
No
Yes
Atha\AT1G17790
2 of 13
No
No
Atha\NPX1
2 of 13
No
No
Atha\AT1G05910
1 of 13
No
No
Atha\AT1G58025
1 of 13
No
No
Atha\AT4G16215
1 of 13
No
Yes
Atha\AT5G08630
1 of 13
No
No
Atha\AT5G46550
1 of 13
No
No
Atha\BET9
1 of 13
No
No
Atha\BET10
1 of 13
No
No
Atha\BT1
1 of 13
No
Yes
Atha\BT2
1 of 13
No
Yes
Atha\BT3
1 of 13
No
No
Atha\BT4
1 of 13
No
Yes
Atha\BT5
1 of 13
No
Yes
Atha\GTE2
1 of 13
No
No
Atha\GTE3
1 of 13
No
No
Atha\GTE4
1 of 13
No
No
Atha\GTE6
1 of 13
No
No
Atha\GTE7
1 of 13
No
No
Atha\GTE8
1 of 13
No
No
Atha\HAG1
1 of 13
No
No
Atha\IMB1
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (9)
Scer\BDF1
2 of 13
Yes
No
Scer\BDF2
2 of 13
Yes
No
Scer\GCN5
1 of 13
No
No
1  
Scer\ITC1
1 of 13
No
No
Scer\RSC1
1 of 13
No
No
Scer\RSC2
1 of 13
No
No
Scer\RSC4
1 of 13
No
No
Scer\SPT7
1 of 13
No
No
Scer\YPL216W
1 of 13
No
Yes
Schizosaccharomyces pombe (Fission yeast) (10)
Spom\bdf1
3 of 12
Yes
No
Spom\SPAC631.02
3 of 12
Yes
No
Spom\bdc1
2 of 12
No
Yes
Spom\gcn5
2 of 12
No
No
Spom\rsc1
1 of 12
No
No
Spom\rsc4
1 of 12
No
No
Spom\snf21
1 of 12
No
No
Spom\snf22
1 of 12
No
No
Spom\SPBP35G2.11c
1 of 12
No
No
Spom\spt7
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:nej. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (12)
fs(1)h
2 of 13
polybromo
2 of 13
Acf
1 of 13
Brd8
1 of 13
dikar
1 of 13
Gcn5
1 of 13
Kcmf1
1 of 13
Lpt
1 of 13
tbrd-1
1 of 13
tbrd-2
1 of 13
tbrd-3
1 of 13
tou
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 4 )
Potential Models Based on Orthology ( 3 )
Modifiers Based on Experimental Evidence ( 20 )
Allele
Disease
Interaction
References
nejQ7
ameliorates  Schimke immuno-osseous dysplasia
modeled by Marcal1UAS.cBa
(Morimoto et al., 2016)
nej3
exacerbates  Huntington's disease
modeled by Hsap\HTTQ93.ex1.UAS
(Bodai et al., 2012)
exacerbates  acute myeloid leukemia
modeled by Hsap\HOXA9::Hsap\NUP98WT.UAS
(Gavory et al., 2021)
nejUAS.Tag:V5
exacerbates  Machado-Joseph disease
modeled by Hsap\ATXN3tr.Q27.UAS.Tag:HA
(Cho et al., 2022)
ameliorates  Alzheimer's disease
modeled by Hsap\APPAβ42.UAS.cUa
(Cutler et al., 2015)
ameliorates  acute myeloid leukemia
modeled by Hsap\HOXA9::Hsap\NUP98WT.UAS
(Gavory et al., 2021)
nejKIX.UAS
ameliorates  Alzheimer's disease
modeled by Hsap\APPAβ42.UAS.cUa
(Cutler et al., 2015)
nejRNAi.UAS.cKa
exacerbates  Machado-Joseph disease
modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
(Raj and Sarkar, 2019)
nejF2161A.UAS.Tag:V5
ameliorates  Machado-Joseph disease
modeled by Hsap\ATXN3tr.Q27.UAS.Tag:HA
(Cho et al., 2022)
nejEP950
exacerbates  autosomal dominant cerebellar ataxia
modeled by Hsap\ATXN7102Q.T.UAS.Tag:NLS(SV40-largeT)
(Latouche et al., 2007)
nejΔBHQ.UAS
exacerbates  Alzheimer's disease
modeled by Hsap\APPAβ42.UAS.cUa
(Cutler et al., 2015)
exacerbates  Machado-Joseph disease
modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
(Raj and Sarkar, 2019)
nejΔHQ.UAS
ameliorates  Alzheimer's disease
modeled by Hsap\APPAβ42.UAS.cUa
(Cutler et al., 2015)
nejhs.PA
model of  alcohol use disorder
is ameliorated by sloMI02233
(Ghezzi et al., 2017)
nejΔNZK.UAS
ameliorates  Alzheimer's disease
modeled by Hsap\APPAβ42.UAS.cUa
(Cutler et al., 2015)
nejΔQ.UAS
ameliorates  Alzheimer's disease
modeled by Hsap\APPAβ42.UAS.cUa
(Cutler et al., 2015)
nejP
exacerbates  neurodegenerative disease
modeled by Zzzz\CAG127Q.UAS.Tag:HA
(Taylor et al., 2003)
ameliorates  autosomal dominant cerebellar ataxia
modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
(Jung and Bonini, 2007)
modeled by Hsap\ATXN3tr.Q46.UAS
(Jung and Bonini, 2007)
nejEP1410
ameliorates  neurodegenerative disease
modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
(Huen and Chan, 2005)
nejGD8975
ameliorates  Huntington's disease
modeled by Hsap\HTTN548.Q128.UAS
(Martin et al., 2021)
nejKK113191
ameliorates  Machado-Joseph disease
modeled by Hsap\ATXN3tr.Q27.UAS.Tag:HA
(Cho et al., 2022)
ameliorates  Huntington's disease
modeled by Hsap\HTTN548.Q128.UAS
(Martin et al., 2021)
nejHMS01507
ameliorates  Huntington's disease
modeled by Hsap\HTTN548.Q128.UAS
(Martin et al., 2021)
nejEP1149
ameliorates  neurodegenerative disease
modeled by Zzzz\CAG127Q.UAS.Tag:HA
(Taylor et al., 2003)
nejEP1179
ameliorates  neurodegenerative disease
modeled by Zzzz\CAG127Q.UAS.Tag:HA
(Taylor et al., 2003)
exacerbates  autosomal dominant cerebellar ataxia
modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
(Jung and Bonini, 2007)
modeled by Hsap\ATXN3tr.Q46.UAS
(Jung and Bonini, 2007)
nejRNAi.UAS.cSa
ameliorates  Huntington's disease
modeled by Hsap\HTTN548.Q128.UAS
(Martin et al., 2021)
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
EP300; EP300 lysine acetyltransferase
12 of 14
602700
CREBBP; CREB binding lysine acetyltransferase
11 of 14
600140
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Interaction Browsers
View in FlyBase Interactions Browser   View in MIST tool (external link)
Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
nej
enzymatic study, western blot
(Tie et al., 2016)
nej - CG14451
anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
(Rhee et al., 2014)
nej - Clk
anti bait coimmunoprecipitation, autoradiography
(Hung et al., 2007)
nej - EcR
anti tag coimmunoprecipitation, anti tag western blot
(Kirilly et al., 2011)
nej - HDAC1
anti bait coimmunoprecipitation, western blot
(Tie et al., 2016)
nej - His3
histone acetylase assay, western blot
(Tie et al., 2012)
nej - HnRNP-K
anti bait coimmunoprecipitation, western blot
(Mallik and Lakhotia, 2010)
nej - Hrb87F
anti bait coimmunoprecipitation, western blot
(Mallik and Lakhotia, 2010)
nej - JIL-1
anti bait coimmunoprecipitation, western blot
(Kellner et al., 2012)
nej - Jra
enzymatic study, western blot
(Zhang et al., 2013)
nej - Lpt
anti tag coimmunoprecipitation, western blot
(Zraly et al., 2020)
nej - MEP-1
anti bait coimmunoprecipitation, Identification by mass spectrometry
(Reddy et al., 2010)
nej - Mad
pull down, autoradiography, two hybrid
(Lilja et al., 2007, Waltzer and Bienz, 1999)
nej - Mef2
two hybrid
(Lin and Baines, 2019)
nej - Mi-2
anti bait coimmunoprecipitation, Identification by mass spectrometry
(Reddy et al., 2010)
nej - Pc
anti bait coimmunoprecipitation, western blot, pull down, enzymatic study
(Tie et al., 2016)
nej - Polr2A
anti bait coimmunoprecipitation, western blot
(Tie et al., 2016)
nej - Psc
anti bait coimmunoprecipitation, western blot
(Tie et al., 2016)
nej - Sbf
ion exchange chromatography, molecular sieving, peptide massfingerprinting, western blot
(Petruk et al., 2001)
nej - Sirt1
anti bait coimmunoprecipitation, western blot, pull down, autoradiography
(Smolik, 2009)
nej - TfIIB
anti bait coimmunoprecipitation, anti tag western blot
(Boija et al., 2017)
nej - Utx
pull down, western blot, anti bait coimmunoprecipitation, molecular sieving, anti tag western blot
(Tie et al., 2012)
nej - arm
pull down, western blot, anti tag coimmunoprecipitation, anti tag western blot, autoradiography, anti bait coimmunoprecipitation
(Li et al., 2007)
nej - asf1
anti bait coimmunoprecipitation, western blot
(Das et al., 2009)
nej - bcd
pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot
(Fu et al., 2004)
nej - brm
pull down, anti tag western blot, western blot, anti bait coimmunoprecipitation, molecular sieving
(Tie et al., 2012)
nej - ci
anti tag coimmunoprecipitation, anti tag western blot, two hybrid, lambda phage display, pull down, molecular weight estimation by staining
(Han et al., 2019, Han et al., 2015, Chen et al., 2000, Akimaru et al., 1997)
nej - dl
anti bait coimmunoprecipitation, western blot
(Holmqvist et al., 2012)
nej - en
anti tag coimmunoprecipitation, anti tag western blot
(Luo et al., 2017)
nej - gcm
anti bait coimmunoprecipitation, anti tag western blot, electrophoretic mobility shift assay, autoradiography
(Flici et al., 2014, Sorge et al., 2012)
nej - mei-41
anti tag coimmunoprecipitation, anti tag western blot
(Smolik and Jones, 2007)
nej - repo
anti bait coimmunoprecipitation, western blot
(Flici et al., 2014)
nej - trr
anti bait coimmunoprecipitation, western blot
(Tie et al., 2014)
nej - trx
anti bait coimmunoprecipitation, western blot, ion exchange chromatography, molecular sieving, peptide massfingerprinting, pull down, anti tag western blot
(Tie et al., 2014, Petruk et al., 2001)
RNA-protein
Physical Interaction
Assay
References
nej - Fmr1
anti tag coimmunoprecipitation, reverse transcription pcr
(Li and Gavis, 2022)
Summary of Genetic Interactions
Interaction Browsers
View in FlyBase Interactions Browser   View in MIST tool (external link)
Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
nej
suppressible
arm
(Waltzer and Bienz, 1998)
nej
suppressible
ash1
(Bantignies et al., 2000)
nej
enhanceable
cgd
(Anderson et al., 2005)
nej
suppressible
cgd
(Anderson et al., 2005)
nej
suppressible
CrebA
(Anderson et al., 2005)
nej
enhanceable
CrebA
(Anderson et al., 2005)
nej
suppressible
CrebB
(Anderson et al., 2005)
nej
enhanceable
dpp
(Anderson et al., 2005)
nej
suppressible
E(z)
(Tie et al., 2009)
nej
enhanceable
Egfr
(Anderson et al., 2005)
nej
suppressible
enc
(Anderson et al., 2005)
nej
suppressible
ey
(Anderson et al., 2005)
nej
enhanceable
eya
(Anderson et al., 2005)
nej
suppressible
Fmr1
(Li and Gavis, 2022)
nej
suppressible
fru
(Sethi et al., 2019)
nej
suppressible
gl
(Anderson et al., 2005)
nej
suppressible
Glo1
(Anderson et al., 2005)
nej
enhanceable
kni
(Anderson et al., 2005)
nej
enhanceable
Kr
(Anderson et al., 2005)
nej
suppressible
l(3)71DEc
(Anderson et al., 2005)
nej
suppressible
l(3)72Df
(Anderson et al., 2005)
nej
suppressible
l(3)L3659
(Anderson et al., 2005)
nej
suppressible
lilli
(Anderson et al., 2005)
nej
suppressible
lncRNA:Hsrω
(Mallik and Lakhotia, 2010)
nej
enhanceable
lncRNA:Hsrω
(Mallik and Lakhotia, 2010)
nej
suppressible
luc
(Anderson et al., 2005)
nej
enhanceable
mamo
(Nakamura et al., 2020)
nej
suppressible
mir-276a
(Li and Gavis, 2022)
nej
suppressible
mir-276b
(Li and Gavis, 2022)
nej
suppressible
Nrg
(Zhang et al., 2014)
nej
enhanceable
Pfas
(Anderson et al., 2005)
nej
enhanceable
Phm
(Anderson et al., 2005)
nej
suppressible
Pka-R1
(Bantignies et al., 2000)
nej
enhanceable
rho
(Anderson et al., 2005)
nej
suppressible
RhoGAP93B
(Anderson et al., 2005)
nej
suppressible
rn
(Anderson et al., 2005)
nej
enhanceable
Scr
(Anderson et al., 2005)
nej
enhanceable
sgg
(Waltzer and Bienz, 1998)
nej
enhanceable
sog
(Anderson et al., 2005)
nej
enhanceable
spi
(Anderson et al., 2005)
nej
suppressible
srp
(Anderson et al., 2005)
nej
suppressible
sty
(Anderson et al., 2005)
nej
suppressible
Tpl
(Anderson et al., 2005)
nej
suppressible
Tsp42Ea
(Anderson et al., 2005)
nej
enhanceable
Ubx
(Anderson et al., 2005)
nej
enhanceable
Xrp1
(Anderson et al., 2005)
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
ci
suppressible
nej
(Florence and McGinnis, 1998, Akimaru et al., 1997)
ci
enhanceable
nej
(Florence and McGinnis, 1998)
Dfd
enhanceable
nej
(Florence and McGinnis, 1998)
dpp
enhanceable
nej
(Waltzer and Bienz, 1999)
Fmr1
suppressible
nej
(Li and Gavis, 2022)
gcm
suppressible
nej
(Popkova et al., 2012)
H
enhanceable
nej
(Müller et al., 2005)
H
suppressible
nej
(Müller et al., 2005)
krz
enhanceable
nej
(Bantignies et al., 2002)
L
enhanceable
nej
(Singh et al., 2005)
mamo
enhanceable
nej
(Nakamura et al., 2020)
mir-276a
suppressible
nej
(Li and Gavis, 2022)
mir-276b
suppressible
nej
(Li and Gavis, 2022)
Myb
enhanceable
nej
(Fung et al., 2003)
Myb
suppressible
nej
(Okada et al., 2002)
Nsf2
suppressible
nej
(Laviolette et al., 2005)
Pc
enhanceable
nej
(Tie et al., 2009)
qed
enhanceable
nej
(Florence, 1999.7.22)
sax
suppressible
nej
(Waltzer and Bienz, 1999)
shg
suppressible
nej
(Waltzer and Bienz, 1998)
so
suppressible
nej
(Kumar et al., 2004)
so
enhanceable
nej
(Kumar et al., 2004)
Ubx
enhanceable
nej
(Florence and McGinnis, 1998)
External Data
Linkouts
DroID - A comprehensive database of gene and protein interactions.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
FlyBase
External Links
Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
External Data
Class of Gene
Genomic Location and Detailed Mapping Data
Chromosome (arm)
X
Recombination map
1-29
Cytogenetic map
8F7-8F9
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
8F7-8F9
Limits computationally determined from genome sequence between P{EP}EP912EP912 and P{EP}nejEP1149&P{EP}nejEP1179
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
8F4-8F8
(determined by in situ hybridisation)
(Peter et al., 2002)
8F4-8F7
(determined by in situ hybridisation)
(Schäfer et al., 1999.6.15, Schäfer et al., 1999.12.2)
8F-9A
(Chen, 1997.2.5)
8F6-8F7
(determined by in situ hybridisation)
(Schäfer et al., 1999.8.12)
8F-9A
(determined by in situ hybridisation)
(Akimaru et al., 1997)
Experimentally Determined Recombination Data
Location

1-29

(FlyBase, 2016)

1-30.6

(Comeron et al., 2012)
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (34)
Genomic Clones (23)
cDNA Clones (26)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
Antibody Information
Cell Line Information
Publicly Available Cell Lines
 
    Other Stable Cell Lines
     
    Other Comments

    Overexpression of nej can repress or activate wg signaling depending on the context.

    (Li et al., 2007)

    RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

    (Boutros et al., 2004)

    One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.

    (Inlow and Restifo, 2004)

    nej functions in eye specification. In addition, nej has a role in cell fate determination during eye development, in particular in R3/R4 cell fate choice and also probably in the specification of the R7 photoreceptor.

    (Kumar et al., 2004)

    nej and Myb work together to promote efficient progression through mitosis.

    (Fung et al., 2003)

    Reduction in maternally contributed nej protein levels in embryos leads to reductions in expression of tld, scw, tsg and rho, but not dpp, in early embryogenesis. rho expression is even lost in cells containing signal dependent phosphorylated Mad protein (a marker of BMP signaling).

    (Lilja et al., 2003)

    nej function is necessary for ci-mediated transactivation of wg during embryogenesis.

    (Chen et al., 2000)

    Postsynaptic nej is necessary for normal presynaptic functional development.

    (Marek et al., 2000)

    nej functions as a co-activator during dpp signalling. Mad may recruit nej to effect the transcriptional activation of dpp responsive genes during development.

    (Waltzer and Bienz, 1999)

    The nej gene product represses pan to antagonise wg signalling.

    (Waltzer and Bienz, 1998)

    nej is necessary for dl-mediated activation of the twi promoter.

    (Akimaru et al., 1997)

    Isolated from a genomic library using a fragment of the C.elegans CBP gene as a probe, under low stringency conditions.

    (Akimaru et al., 1997)

    nej has been cloned and characterised.

    (Akimaru et al., 1997)

    Using the co-transfection assay with the nej expression plasmid results demonstrate trans-activation by Myb and functional domains of Myb involved in this activity are identified.

    (Hou et al., 1997)
    Relationship to Other Genes
    Source for database merge of

    Source for merge of: nej CG15319

    (Bayraktaroglu, 2000.4.12)

    Source for merge of: nej l(1)G0350 l(1)G0112 l(1)G0470 CG15321

    (Yin et al., 2007)

    Source for merge of: nej anon-WO03040301.89

    (FlyBase, 1996-)
    Additional comments

    Annotations CG15321 eliminated in release 5.25 of the genome annotation.

    (FlyBase Genome Annotators, 2010)

    FlyBase curator comment: "EP1410" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise.

    (Müller et al., 2005)

    FlyBase curator comment: "EP950" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise. FlyBase curator comment: "EP1410" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise.

    (Singh et al., 2005)

    FlyBase curator comment: "EP950" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise.

    (Pena-Rangel et al., 2002)

    "l(1)G0464" may affect "nej".

    (Peter et al., 2002)

    "l(1)G0350" may affect "CG15321".

    (Peter et al., 2002)

    "l(1)G0112" may affect "CG15321".

    (Peter et al., 2002)

    "l(1)G0470" may affect "CG15321".

    (Peter et al., 2002)

    "l(1)G0350" may affect "CG15321". "l(1)G0286" may affect "CG15321". "l(1)G0137" may affect "CG15321". "l(1)G0112" may affect "CG15321". "l(1)G0270" may affect "CG15321". "l(1)G0470" may affect "CG15321". "l(1)G0236" may affect "CG15321".

    (Peter et al., 2002)

    Source for merge of nej anon-WO03040301.89 was sequence comparison ( date:051113 ).

    (FlyBase, 1996-)
    Nomenclature History
    Source for database identify of
    Nomenclature comments
    Etymology

    'nejire' means 'twist' in Japanese.

    (Akimaru et al., 1997)
    Synonyms and Secondary IDs (37)
    Reported As
    Symbol Synonym
    CBP
    (Marsh et al., 2025, Krasnov et al., 2023, Cho et al., 2022, Nisha and Sarkar, 2022, Yoneda et al., 2022, Furusawa and Emoto, 2021, Mazina et al., 2021, Takakura et al., 2021, Duffraisse et al., 2020, Nakamura et al., 2020, Zraly et al., 2020, Gutierrez-Perez et al., 2019, Haberle et al., 2019, Han et al., 2019, Raj and Sarkar, 2019, Sajwan and Mannervik, 2019, Boija et al., 2017, Chung et al., 2017, Ghezzi et al., 2017, Song et al., 2017, Doiguchi et al., 2016, Flegel et al., 2016, Hirano et al., 2016, Lee et al., 2016, Tie et al., 2016, Baëza et al., 2015, Cutler et al., 2015, Dietz et al., 2015, Ho et al., 2014, Singh et al., 2014, Tie et al., 2014, Zhang et al., 2014, Darbo et al., 2013, Gisselbrecht et al., 2013, Hirano et al., 2013, Zhang et al., 2013, Bodai et al., 2012, Holmqvist et al., 2012, Kellner et al., 2012, Tie et al., 2012, Kirilly et al., 2011, Nègre et al., 2011, Pruteanu-Malinici et al., 2011, Schuettengruber et al., 2011, Boyles et al., 2010, Herz et al., 2010, Mallik and Lakhotia, 2010, Reddy et al., 2010, Das et al., 2009, Smolik, 2009, Tie et al., 2009, Fanti et al., 2008, Parker et al., 2008, Tavantzis and Warrick, 2008, Wang et al., 2008, Fortini, 2007, Hung et al., 2007, Latouche et al., 2007, Li et al., 2007, Lim et al., 2007, Parker et al., 2007, Yin et al., 2007, Parker et al., 2006, Anderson et al., 2005, Eissenberg et al., 2005, Fernandez-Funez et al., 2005, Huen and Chan, 2005, Ma, 2005, Kumar et al., 2004, Lipsick, 2004, Ogden et al., 2004, Sanchez-Elsner and Sauer, 2004, Smith et al., 2004, Yang, 2004, Fernandez-Funez et al., 2003, Petruk et al., 2003, Ingham and McMahon, 2001, Sharpe et al., 2001, Attisano and Wrana, 2000, Chen and Courey, 2000, Glass and Rosenfeld, 2000, Goodman and Smolik, 2000, Kouzarides, 1999, Mannervik et al., 1999, Matise and Joyner, 1999, Murone et al., 1999, Novak and Dedhar, 1999, Anonymous, 1998, Ingham, 1998, Chen, 1997.2.5, Tabin and McMahon, 1997)
    CBP/p300
    (Mazina et al., 2019, Fei et al., 2015, Stadeli, 2006, Newfeld and Takaesu, 2002, Newfeld et al., 2002, Vo and Goodman, 2001, Behrens, 2000, Seidensticker and Behrens, 2000)
    CBP_
    (Bashirullah et al., 2006)
    CG15319
    (Lin et al., 2018, Hosono et al., 2015, Ni et al., 2011.7.19, Reddy et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Grieder et al., 2007, Laviolette et al., 2005, Ramet et al., 2002)
    CG15321
    (FlyBase Genome Annotators, 2010, Popodi et al., 2010-, Venken et al., 2010, Keleman et al., 2009.8.5, Ni et al., 2008, Stofanko et al., 2008, Grieder et al., 2007, Grieder et al., 2007, Levine et al., 2007, Yin et al., 2007, Manak et al., 2006)
    Cbp
    (Chen et al., 1998, Chen et al., 1996)
    Crbp
    Nej
    (Martinez, 2025, Kang et al., 2022, Ramirez Moreno et al., 2021, Wei et al., 2020, Kwon et al., 2018, Ramirez-Roman et al., 2018, Mbodj et al., 2013, van Eyk et al., 2011, Zhou et al., 2011, Anholt and Williams, 2010, Guenin et al., 2010, Martin et al., 2007, Yin et al., 2007)
    Nejire
    (Krasnov et al., 2023, Pang et al., 2023, Reddy et al., 2010)
    P300
    (Long et al., 2025, Haberle et al., 2019)
    anon-WO0147981.11
    (Modjtahedi, 2001.7.5)
    anon-WO03040301.89
    cbp
    (Maurer et al., 2016, Ayscough, 1998)
    dCBP
    (Cho and O'Farrell, 2023, Martin et al., 2021, Sethi et al., 2019, Li et al., 2018, Han et al., 2015, Flici et al., 2014, Bodai et al., 2012, Holmqvist et al., 2012, Ito et al., 2012, Zhai et al., 2012, Crump et al., 2011, Boyles et al., 2010, Smolik, 2009, Zhao et al., 2009, Hong et al., 2008, Tavantzis and Warrick, 2008, Jung and Bonini, 2007, Lilja et al., 2007, Nagraj et al., 2007, Nibu et al., 2007, Schuettengruber et al., 2007, Smolik and Jones, 2007, Petruk et al., 2006, Fu and Ma, 2005, Fu et al., 2004, Kumar et al., 2004, Kwon et al., 2004, Li et al., 2004, Smith et al., 2004, Evans et al., 2003, Fung et al., 2003, Li et al., 2003, Lilja et al., 2003, Lilja et al., 2003, Maves and Schubiger, 2003, Taylor et al., 2003, Bantignies et al., 2002, Cavarec et al., 2002, Jia et al., 2002, Ludlam et al., 2002, Newman et al., 2002, Simon and Tamkun, 2002, Fry and Peterson, 2001, Ludlam et al., 2001, Petruk et al., 2001, Petruk et al., 2001, Bantignies et al., 2000, Chen et al., 2000, Davis et al., 2000, Ludlam et al., 2000, Marek et al., 2000, Aza-Blanc and Kornberg, 1999, Bantignies et al., 1999, Govind, 1999, Newman et al., 1999, Roose and Clevers, 1999, Vousden, 1999, Anonymous, 1998, Bantignies et al., 1998, Giles et al., 1998, Goodrich and Scott, 1998, Miska, 1998, Waltzer and Bienz, 1998, Akimaru et al., 1997, Akimaru et al., 1997, Florence and McGinnis, 1997, Hou et al., 1997)
    dKAT3
    (Crump et al., 2011)
    dmCBP
    (Pandey et al., 2002)
    l(1)G0112
    l(1)G0350
    l(1)G0470
    nej
    (Cho and O'Farrell, 2025, Jang et al., 2024, Li et al., 2024, Willnow and Teleman, 2024, Nieken et al., 2023, Titlow et al., 2023, Tu et al., 2023, Di Fede et al., 2022, Ding et al., 2022, Falo-Sanjuan and Bray, 2022, Li and Gavis, 2022, Miao et al., 2022, Velten et al., 2022, Bilder et al., 2021, Gavory et al., 2021, Martin et al., 2021, Raun et al., 2021, Aboukilila et al., 2020, Cho et al., 2020, Graves et al., 2020, Hung et al., 2020, Jeon et al., 2020, Park et al., 2020, Zhao et al., 2020, Nakamura et al., 2019, Sang Cho et al., 2019, Muñoz-Soriano et al., 2018, Rust et al., 2018, Salazar and Yamamoto, 2018, Ghezzi et al., 2017, Janssens et al., 2017, Transgenic RNAi Project members, 2017-, Vallejos Baier et al., 2017, Brewer-Jensen et al., 2016, Malartre, 2016, Maurer et al., 2016, Morimoto et al., 2016, Moulton and Letsou, 2016, Schwartz et al., 2016, Yu et al., 2016, Jia et al., 2015, Lee et al., 2015, Ugrankar et al., 2015, Vedanayagam and Garrigan, 2015, Flici et al., 2014, Rogers et al., 2014, Yan et al., 2014, Czech et al., 2013, de Celis et al., 2013.9.11, Ghezzi et al., 2013, Neumüller et al., 2013, Tögel et al., 2013, Vaque et al., 2013, Yamamoto et al., 2013-, Bodai et al., 2012, Busser et al., 2012, Hainaut et al., 2012, Hilgers et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Legent et al., 2012, Popkova et al., 2012, Zhai et al., 2012, Hilgers et al., 2011, Kirilly et al., 2011, Smolik, 2011.2.28, Toku et al., 2011, He et al., 2010, Pospisilik et al., 2010, Sela et al., 2010, Smolik, 2010.11.15, Stofanko et al., 2010, Ayroles et al., 2009, Dworkin et al., 2009, Stofanko et al., 2008, Straub et al., 2008, Gilfillan et al., 2007, Grieder et al., 2007, Grieder et al., 2007, Hung et al., 2007, Li et al., 2007, Lilja et al., 2007, Nagraj et al., 2007, Smolik and Jones, 2007, Weber et al., 2007, Friedman and Perrimon, 2006, DasGupta et al., 2005, Huen and Chan, 2005, Laviolette et al., 2005, Ito et al., 2004, Stanyon et al., 2004, Takeyama et al., 2004, Petruk et al., 2001)
    nej CBP
    (Singh et al., 2005)
    nejire
    (Peterson and Ahmad, 2024, Erokhin et al., 2023, Shimaji et al., 2019)
    p300
    (Krasnov et al., 2023, Su et al., 2019, Fei et al., 2015, Vaqué et al., 2013, Nègre et al., 2011, Lee and Stallcup, 2006)
    p300/CBP
    (Hunt and Mannervik, 2024, Haberle et al., 2019, Yeung et al., 2011, Lee and Stallcup, 2006, Mizzen and Allis, 2000)
    Name Synonyms
    CBP histone acetyltransferase
    (Chetverina et al., 2021)
    CBP/Nejire
    (Yin et al., 2007)
    CREB Binding Protein
    (Cutler et al., 2015)
    CREB binding protein
    (Gisselbrecht et al., 2013, Kellner et al., 2012, Nègre et al., 2011, Zhao et al., 2009, Hart and Warrick, 2008, Tavantzis and Warrick, 2008, Yin et al., 2007, Park et al., 2001, Ingham, 1998)
    CREB-binding protein
    (Marsh et al., 2025, Tie et al., 2016, Kirilly et al., 2011, Boyles et al., 2010, Smolik, 2009, Parker et al., 2008, Fortini, 2007, Johnston and Scott, 1998, Chen et al., 1996)
    Creb binding protein
    (Flici et al., 2014)
    Cyclic AMP Response Element Binding protein
    (Smolik, 2009)
    Drosophila CREB-binding
    (Kwon et al., 2004)
    Drosophila CREB-binding protein
    (Nibu et al., 2007)
    Nejire
    (Chetverina et al., 2020, Schmidt et al., 2011, Das et al., 2009, Martin et al., 2007, Yin et al., 2007)
    lethal (1) G0112
    lethal (1) G0350
    lethal (1) G0470
    nejire
    (Amartuvshin et al., 2025, Klose and Shaw, 2021, Saunders, 2021, Graves et al., 2020, Jiang et al., 2019, Salazar and Yamamoto, 2018, Malartre, 2016, Maurer et al., 2016, Schwartz et al., 2016, Dietz et al., 2015, Vedanayagam and Garrigan, 2015, Seridi et al., 2014, Tögel et al., 2013, Bodai et al., 2012, Bodai et al., 2012, Holmqvist et al., 2012, Legent et al., 2012, Mallik and Lakhotia, 2010, Fanti et al., 2008, Stofanko et al., 2008, Wang et al., 2008, Li et al., 2007, Lilja et al., 2007, Olson et al., 2007, Pilot et al., 2006, Petruk et al., 2001, Akimaru et al., 1997)
    protein
    (Kwon et al., 2004)
    Secondary FlyBase IDs
    • FBgn0015624
    • FBgn0030149
    • FBgn0066809
    • FBgn0028319
    • FBgn0027307
    • FBgn0040152
    • FBgn0030150
    Datasets (1)
    Study focus (1)
    Experimental Role
    Project
    Project Type
    Title
    • bait_protein
    modENCODE_regulation_chromatin
    Genome-wide localization of chromatin factors by ChIP-chip and ChIP-Seq.
    Study result (0)
    Result
    Result Type
    Title
    External Crossreferences and Linkouts ( 102 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    MARRVEL_MODEL - MARRVEL (model organism gene)
    Linkouts
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    References (412)