Gene model reviewed during 5.49
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.44
Stop-codon suppression (UGA) postulated; FBrf0216884
Gene model reviewed during 6.02
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RanBPM using the Feature Mapper tool.
An antibody directed against the long isoform of RanBPM detects expression in third instar larvae in a bilateral dorsal cluster of neurons that colocalize with the Scer\GAL4Toll-6-D42 mushroom body marker as well as in the ventral nerve cord, the ring glands, and in muscles attached to the mouth hooks. No expression is seen in the proliferating centers of the optic lobe, in photoreceptors, in glial cells, or in neuromuscular junction.
RanBPM protein is expressed at the anterior tip of the germarium in the germline stem cells and in dividing cysts. Expression is reduced in the germline as individual egg chambers are formed. In later stages, it becomes enriched in the nuclei of follicle cells and germline cells and at the nurse cell membranes. The long RanBPM isoform (detected with an isoform-specific antibody) is detected in cap cells and in the terminal filament.
GBrowse - Visual display of RNA-Seq signalsView Dmel\RanBPM in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: RanBPM CG42236
Source for merge of: RanBPM l(2)k05201
The CG11763 annotation was originally named "midlife crisis (micr)" (see FBrf0182705) since the first alleles generated had a gradual germline stem cell (GSC) loss phenotype. A second site mutation has since been identified on the "micr" chromosomes, and it has been shown that loss of CG11763 does not cause a GSC loss phenotype. The second site mutations that cause GSC loss are referred to by the name "micr", while the alleles of CG11763 will be published under the gene name "Ran binding protein M (RanBPM)".
FlyBase curator comment: to prevent confusion, the CG11763 annotation has been renamed CG42236.
The long isoform of RanBPM functions in the germline stem cell niche to regulate its organisation and cell size.
The short isoform of RanBPM functions in the germline stem cell niche to suppress adhesion between the cap cells and the germline stem cells.