FB2025_05 , released December 11, 2025
Gene: Dmel\Mi-2
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General Information
Symbol
Dmel\Mi-2
Species
D. melanogaster
Name
Mi-2
Annotation Symbol
CG8103
Feature Type
protein_coding_gene
FlyBase ID
FBgn0262519
Gene Model Status
Current
Stock Availability
36 publicly available
Gene Summary
Mi-2 (Mi-2) encodes a nuclear ATP-dependent nucleosome remodeler of the CHD family. It associates with active chromatin and utilizes the energy of ATP hydrolysis to move nucleosomes along DNA. It is required for repression of cell type-specific genes, full activation of heat shock genes and regulates higher order chromatin structure of polytene chromosomes. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

dMi-2, Pha, dMi2, PHD-containing ATPase, l(3)S085401

Key Links
Genomic Location
Cytogenetic map
76D3-76D4
Sequence location
Recombination map
3-46
RefSeq locus
NT_037436 REGION:19882051..19910215
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (25 terms)
Molecular Function (9 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
enables ATP hydrolysis activity
inferred from direct assay
(Murawska et al., 2008)
enables ATP-dependent chromatin remodeler activity
inferred from direct assay
(Kunert et al., 2009, Murawska et al., 2008)
enables chromatin binding
inferred from direct assay
(Kunert et al., 2009)
enables nucleosome array spacer activity
inferred from direct assay
(Bouazoune and Brehm, 2005, Bouazoune et al., 2002)
enables protein binding
inferred from physical interaction with FLYBASE:MEP-1; FB:FBgn0035357
(Kunert et al., 2009)
inferred from physical interaction with FLYBASE:simj; FB:FBgn0010762
(Kon et al., 2005)
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR000330
(InterPro Project Members, 2004-)
enables ATP hydrolysis activity
inferred from biological aspect of ancestor with PANTHER:PTN002473914
(GO Reference Genome Project, 2011-)
enables ATP-dependent chromatin remodeler activity
inferred from biological aspect of ancestor with PANTHER:PTN002473914
(GO Reference Genome Project, 2011-)
enables chromatin binding
inferred from biological aspect of ancestor with PANTHER:PTN002473914
(GO Reference Genome Project, 2011-)
enables DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN002473914
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR009462
(InterPro Project Members, 2004-)
enables histone binding
inferred from biological aspect of ancestor with PANTHER:PTN002473914
(GO Reference Genome Project, 2011-)
enables zinc ion binding
inferred from sequence model
(Ying et al., 2011)
Biological Process (10 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
involved_in chromosome condensation
inferred from mutant phenotype
(Nikalayevich and Ohkura, 2015)
involved_in chromosome organization
inferred from mutant phenotype
(Fasulo et al., 2012)
involved_in mitotic sister chromatid cohesion
inferred from mutant phenotype
(Fasulo et al., 2012)
involved_in negative regulation of DNA-templated transcription
inferred from genetic interaction with UniProtKB:P05084
(Kehle et al., 1998)
involved_in negative regulation of transcription by RNA polymerase II
inferred from genetic interaction with FLYBASE:foxo; FB:FBgn0038197
(Zheng et al., 2025)
inferred from direct assay
(Kunert et al., 2009)
involved_in nucleosome organization
inferred from direct assay
(Murawska et al., 2008, Bouazoune and Brehm, 2005, Bouazoune et al., 2002)
involved_in regulation of transcription by RNA polymerase II
inferred from mutant phenotype
(Li et al., 2010)
involved_in spermatogenesis
inferred from mutant phenotype
(Lindsley et al., 2013)
involved_in transposable element silencing by piRNA-mediated heterochromatin formation
inferred from mutant phenotype
(Mugat et al., 2020)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in chromatin remodeling
inferred from biological aspect of ancestor with PANTHER:PTN002473914
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR009462
(InterPro Project Members, 2004-)
Cellular Component (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in cytosol
inferred from high throughput direct assay
(Lye et al., 2014)
located_in euchromatin
inferred from direct assay
(Murawska et al., 2008)
located_in nucleus
inferred from high throughput direct assay
(Lye et al., 2014)
inferred from direct assay
(Kim et al., 2017, Kunert et al., 2009, Murawska et al., 2008)
located_in polytene chromosome
inferred from direct assay
(Reddy et al., 2010, Murawsky et al., 2001)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
is_active_in chromatin
inferred from biological aspect of ancestor with PANTHER:PTN002473914
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN002473914
(GO Reference Genome Project, 2011-)
part_of NuRD complex
inferred from sequence or structural similarity
(Ahringer, 2000)
traceable author statement
(Murawsky et al., 2001)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the SNF2/RAD54 helicase family. (O97159)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
ATP hydrolysis activity
RHEA 13065:
Summaries
Gene Snapshot
Mi-2 (Mi-2) encodes a nuclear ATP-dependent nucleosome remodeler of the CHD family. It associates with active chromatin and utilizes the energy of ATP hydrolysis to move nucleosomes along DNA. It is required for repression of cell type-specific genes, full activation of heat shock genes and regulates higher order chromatin structure of polytene chromosomes. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
NUCLEOSOME REMODELING DEACETYLASE COMPLEX -
The nucleosome remodelling and deacetylase (NuRD) complex couples chromatin remodelling and histone deacetylation to mediate transcriptional repression. (Adapted from FBrf0228973 and FBrf0228920).
SNF2-LIKE CHROMATIN REMODELERS -
Snf2-like chromatin remodelers are a family of helicase-like proteins that direct energy derived from ATP hydrolysis into the mechanical remodelling of chromatin structure. They are so-called due to the presence of a domain homologous to the helicase-like ATPase domain of the S. cerevisiae Snf2 protein. This domain, the Snf2 domain, consists of two tandem RecA-like folds and contains seven conserved helicase-related sequence motifs that classify it as part of the helicase superfamily 2 (SF2). However, Snf2 proteins are not bona fide helicases, lacking the ability to separate nucleic acid strands. Instead, Snf2 proteins are DNA translocases that apply an ATP-dependent torsional strain to DNA, which provides the necessary force to remodel nucleosomes or in some cases other DNA-protein complexes. (Adapted from PMID:21862382.)
Protein Function (UniProtKB)
ATP-dependent chromatin-remodeling factor which acts in nucleosome-remodeling by catalyzing ATP-dependent nucleosome mobilization (PubMed:18250149). Involved in regulating transcription (PubMed:18250149, PubMed:9836641). Plays a vital role in development (PubMed:9836641). Binds to a portion of Hunchback (HB) protein that is critical for repression of bithorax complex (BXC) genes (PubMed:9836641). May also function in polycomb group (PcG) repression of Hox genes (PubMed:9836641). May also act as part of the nucleosome remodeling and deacetylase complex (the NuRD complex) which participates in the remodeling of chromatin by deacetylating histones (PubMed:18250149).
(UniProt, O97159)
Summary (Interactive Fly)

ATP dependent DNA helicase - Zinc finger, CHD family - associates with active chromatin and utilizes the energy of ATP hydrolysis to move nucleosomes along DNA - required for repression of cell type-specific genes and full activation of heat shock genes - regulates higher order chromatin structure of polytene chromosomes.

Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\Mi-2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry O97159)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.53

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Mi-2-RA
FBtr0074919
NM_140897
6416
1982
Mi-2-RB
FBtr0100394
NM_001014591
6486
1983
Mi-2-RC
FBtr0302046
NM_001170005
6483
1982
Mi-2-RD
FBtr0332152
NM_001275149
6424
1973
Additional Transcript Data and Comments
Reported size (kB)

6.475 (compiled cDNA)

(Kehle et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Mi-2-PA
FBpp0074688
224.2
1982
5.33
O97159
NP_649154
AAF49099
Mi-2-PB
FBpp0099808
224.3
1983
5.33
Q59E34
NP_001014591
AAX52739
Mi-2-PC
FBpp0291256
224.2
1982
5.33
O97159
NP_001163476
ACZ94747
Mi-2-PD
FBpp0304462
223.1
1973
5.48
M9PIA6
NP_001262078
AGB94771
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1982 aa isoforms: Mi-2-PA, Mi-2-PC
Additional Polypeptide Data and Comments
Reported size (kDa)

1982 (aa)

(Kehle et al., 1998)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with the NuRD complex member HDAC1/Rpd3.

(UniProt, O97159)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mi-2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.46

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Kehle et al., 1998)
Additional Descriptive Data

Mi-2 transcript is uniform in preblastoderm embryos.

(Kehle et al., 1998)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunodetection (other than il)
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
wing disc
(Kunert et al., 2009)
larval brain
(Kunert et al., 2009)
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis
nurse cell
(Kunert et al., 2009)
oocyte
(Kunert et al., 2009)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Wasbrough et al., 2010)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 12
organism
(Kunert et al., 2009)
Additional Descriptive Data

Mi-2 protein is detected in the first 9 hours of embryonic development and then levels drop off sharply.

(Kunert et al., 2009)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosol
inferred from high throughput direct assay
(Lye et al., 2014)
located_in euchromatin
inferred from direct assay
(Murawska et al., 2008)
located_in nucleus
inferred from high throughput direct assay
(Lye et al., 2014)
inferred from direct assay
(Kim et al., 2017, Kunert et al., 2009, Murawska et al., 2008)
located_in polytene chromosome
inferred from direct assay
(Reddy et al., 2010, Murawsky et al., 2001)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mi-2 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 60 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Mi-2
Transgenic constructs containing regulatory region of Mi-2
Aberrations (Deficiencies and Duplications) ( 29 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal, with Mi-24, Scer\GAL4da.G32
lethal, with Mi-2Δ932-1158.UAS.Tag:FLAG, Scer\GAL4da.G32
lethal, with Scer\GAL4en.PU
lethal (with Df(3L)Δ40)
lethal | heat sensitive, with Scer\GAL4da.G32
lethal | recessive
lethal - all die before end of larval stage | recessive
partially lethal, with Scer\GAL4da.G32
partially lethal - majority die
partially lethal - majority die, with Mi-2f08103, Scer\GAL4da.G32
partially lethal - majority die, with Mi-2Δ932-1158.UAS.Tag:FLAG, Scer\GAL4da.G32
partially lethal - majority die, with Scer\GAL4ey.PH
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
fertile
Other Phenotypes
Allele
abnormal meiotic cell cycle | adult stage | male, with Scer\GAL4VP16.bam
abnormal neuroanatomy
abnormal size | adult stage, with Scer\GAL4nub.PK
decreased cell size | larval stage | somatic clone, with Scer\GAL4Act.PU
increased cell number | larval stage, with Scer\GAL4wor.PA, Scer\GAL80ase.PN
increased size | adult stage, with Scer\GAL4GMR.PF
short lived, with Scer\GAL4Switch1.106
some die during larval stage, with Scer\GAL4da.G32
some die during larval stage | recessive
visible, with Scer\GAL4bbg-C96
visible | adult stage, with Scer\GAL4ey.PH
visible | adult stage, with Scer\GAL4GMR.PF
visible | adult stage, with Scer\GAL4nub.PK
Phenotype manifest in
Allele
chromosome | adult stage | female, with Scer\GAL4VP16.mat.αTub67C
chromosome | third instar larval stage, with Scer\GAL4da.G32
dendrite
egg | germline clone
embryonic/larval midgut cell | larval stage | somatic clone, with Scer\GAL4Act.PU
eye, with Scer\GAL4ey.PH
eye, with Scer\GAL4GMR.PF
eye photoreceptor cell | somatic clone
larval brain | larval stage, with Scer\GAL4wor.PA, Scer\GAL80ase.PN
male germline cell, with Scer\GAL4VP16.bam
oocyte, with Scer\GAL4VP16.mat.αTub67C
polytene chromosome, with Scer\GAL4da.G32
polytene chromosome puff | heat sensitive, with Scer\GAL4da.G32
retina | somatic clone
spindle | adult stage | female, with Scer\GAL4VP16.mat.αTub67C
type II neuroblast | increased number | larval stage, with Scer\GAL4wor.PA, Scer\GAL80ase.PN
wing, with Scer\GAL4bbg-C96
wing, with Scer\GAL4nub.PK
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (40)
Hsap\CHD5
13 of 14
Yes
Yes
Hsap\CHD3
12 of 14
No
Yes
Hsap\CHD4
12 of 14
No
Yes
Hsap\AIRE
1 of 14
No
No
Hsap\ATRX
1 of 14
No
No
Hsap\BTAF1
1 of 14
No
No
Hsap\CBX1
1 of 14
No
No
2  
Hsap\CBX3
1 of 14
No
No
3  
Hsap\CBX5
1 of 14
No
No
4  
Hsap\CDY1B
1 of 14
No
No
Hsap\CDY1
1 of 14
No
No
Hsap\CDY2A
1 of 14
No
No
Hsap\CDY2B
1 of 14
No
No
Hsap\CDYL2
1 of 14
No
No
1  
Hsap\CDYL
1 of 14
No
No
Hsap\CHD1
1 of 14
No
No
Hsap\CHD1L
1 of 14
No
No
Hsap\CHD2
1 of 14
No
No
Hsap\CHD6
1 of 14
No
No
Hsap\CHD7
1 of 14
No
No
1  
Hsap\CHD8
1 of 14
No
No
Hsap\CHD9
1 of 14
No
No
Hsap\EP400
1 of 14
No
No
1  
Hsap\ERCC6
1 of 14
No
Yes
Hsap\ERCC6L2
1 of 14
No
Yes
Hsap\ERCC6L
1 of 14
No
Yes
Hsap\FSBP
1 of 14
No
No
1  
Hsap\HELLS
1 of 14
No
No
Hsap\PHF21A
1 of 14
No
Yes
Hsap\PHF21B
1 of 14
No
Yes
Hsap\RAD54B
1 of 14
No
No
Hsap\RAD54L2
1 of 14
No
No
Hsap\RAD54L
1 of 14
No
No
Hsap\SMARCA1
1 of 14
No
No
1  
Hsap\SMARCA2
1 of 14
No
No
2  
Hsap\SMARCA4
1 of 14
No
No
Hsap\SMARCA5
1 of 14
No
No
3  
Hsap\SMARCAD1
1 of 14
No
No
Hsap\SMARCAL1
1 of 14
No
No
13  
Hsap\ZRANB3
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (31)
Rnor\Chd5
13 of 14
Yes
Yes
Rnor\Chd4
12 of 14
No
Yes
Rnor\Chd3
11 of 14
No
Yes
Rnor\Aire
1 of 14
No
No
Rnor\Cbx1
1 of 14
No
No
Rnor\Cbx3
1 of 14
No
No
Rnor\Cbx5
1 of 14
No
No
Rnor\Cdyl2
1 of 14
No
No
Rnor\Cdyl
1 of 14
No
No
Rnor\Chd1l
1 of 14
No
No
Rnor\Chd1
1 of 14
No
No
Rnor\Chd2
1 of 14
No
No
Rnor\Chd6
1 of 14
No
No
Rnor\Chd7
1 of 14
No
No
Rnor\Chd8
1 of 14
No
No
Rnor\Chd9
1 of 14
No
No
Rnor\Fsbp
1 of 14
No
No
Rnor\Hells
1 of 14
No
No
Rnor\LOC688980
1 of 14
No
No
Rnor\Mphosph8
1 of 14
No
No
Rnor\Phf21b
1 of 14
No
Yes
Rnor\Rad54b
1 of 14
No
No
Rnor\Rad54l
1 of 14
No
No
Rnor\Smarca1
1 of 14
No
No
Rnor\Smarca2
1 of 14
No
No
Rnor\Smarca4
1 of 14
No
No
Rnor\Smarca5
1 of 14
No
No
Rnor\Smarcad1
1 of 14
No
No
Rnor\Smarcal1
1 of 14
No
No
Rnor\Suv39h2
1 of 14
No
No
Rnor\Zranb3
1 of 14
No
No
Mus musculus (laboratory mouse) (29)
Mmus\Chd3
12 of 14
Yes
Yes
Mmus\Chd4
12 of 14
Yes
Yes
Mmus\Chd5
12 of 14
Yes
Yes
Mmus\Aire
1 of 14
No
No
Mmus\Cbx1
1 of 14
No
No
1  
Mmus\Cbx3
1 of 14
No
No
Mmus\Cbx5
1 of 14
No
No
Mmus\Cdyl
1 of 14
No
No
Mmus\Chd1l
1 of 14
No
No
Mmus\Chd1
1 of 14
No
No
Mmus\Chd2
1 of 14
No
No
Mmus\Chd6
1 of 14
No
No
Mmus\Chd7
1 of 14
No
No
Mmus\Chd8
1 of 14
No
No
Mmus\Chd9
1 of 14
No
No
Mmus\Fsbp
1 of 14
No
No
Mmus\Hells
1 of 14
No
No
Mmus\Mphosph8
1 of 14
No
No
Mmus\Phf21a
1 of 14
No
Yes
Mmus\Phf21b
1 of 14
No
Yes
Mmus\Rad54b
1 of 14
No
No
Mmus\Rad54l
1 of 14
No
No
Mmus\Smarca1
1 of 14
No
No
Mmus\Smarca2
1 of 14
No
No
Mmus\Smarca4
1 of 14
No
No
Mmus\Smarca5
1 of 14
No
No
Mmus\Smarcad1
1 of 14
No
No
Mmus\Smarcal1
1 of 14
No
No
Mmus\Zranb3
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (29)
Xtro\chd3
10 of 13
Yes
Yes
Xtro\chd4
7 of 13
No
Yes
Xtro\chd5
7 of 13
No
Yes
Xtro\cnot7
5 of 13
No
No
Xtro\cbx1
1 of 13
No
No
Xtro\cbx2
1 of 13
No
No
Xtro\cbx3
1 of 13
No
No
Xtro\cdyl2
1 of 13
No
No
Xtro\cdyl
1 of 13
No
No
Xtro\chd1
1 of 13
No
No
Xtro\chd2
1 of 13
No
No
Xtro\chd6
1 of 13
No
No
Xtro\chd7
1 of 13
No
No
Xtro\chd8
1 of 13
No
No
Xtro\chd9
1 of 13
No
No
Xtro\ep400
1 of 13
No
No
Xtro\ercc6l2
1 of 13
No
Yes
Xtro\ercc6l
1 of 13
No
Yes
Xtro\ercc6
1 of 13
No
Yes
Xtro\hells
1 of 13
No
No
Xtro\rad54b
1 of 13
No
No
Xtro\rad54l2
1 of 13
No
No
Xtro\rad54l
1 of 13
No
No
Xtro\smarca2
1 of 13
No
No
Xtro\smarca4
1 of 13
No
No
Xtro\smarcad1
1 of 13
No
No
Xtro\smarcal1
1 of 13
No
No
Xtro\syngr2
1 of 13
No
No
Xtro\ucn3
1 of 13
No
Yes
Danio rerio (Zebrafish) (34)
Drer\chd3
12 of 14
Yes
Yes
Drer\chd4b
12 of 14
Yes
Yes
Drer\chd5
12 of 14
Yes
Yes
Drer\chd4a
11 of 14
No
Yes
Drer\hells
2 of 14
No
No
Drer\btaf1
1 of 14
No
No
Drer\cbx1a
1 of 14
No
No
Drer\cbx1b
1 of 14
No
No
Drer\cbx3a
1 of 14
No
No
Drer\cbx3b
1 of 14
No
No
Drer\cbx5
1 of 14
No
No
Drer\cdyl
1 of 14
No
No
Drer\chd1
1 of 14
No
No
Drer\chd1l
1 of 14
No
No
Drer\chd2
1 of 14
No
No
Drer\chd6
1 of 14
No
No
Drer\chd7
1 of 14
No
No
Drer\chd8
1 of 14
No
No
Drer\chd9
1 of 14
No
No
Drer\ercc6
1 of 14
No
Yes
Drer\LOC100535713
1 of 14
No
No
Drer\mphosph8
1 of 14
No
No
Drer\phf21ab
1 of 14
No
Yes
Drer\rad54b
1 of 14
No
No
Drer\rad54l
1 of 14
No
No
Drer\smarca1
1 of 14
No
No
Drer\smarca2
1 of 14
No
No
Drer\smarca4a
1 of 14
No
No
Drer\smarca5
1 of 14
No
No
Drer\smarcad1a
1 of 14
No
No
Drer\smarcad1b
1 of 14
No
No
Drer\smarcal1
1 of 14
No
No
Drer\suv39h1a
1 of 14
No
No
Drer\zranb3
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (21)
Cele\chd-3
13 of 14
Yes
Yes
Cele\let-418
12 of 14
No
Yes
Cele\rad-54.B
2 of 14
No
Yes
Cele\btf-1
1 of 14
No
No
Cele\cec-3
1 of 14
No
No
Cele\cec-4
1 of 14
No
No
Cele\chd-1
1 of 14
No
No
Cele\chd-7
1 of 14
No
No
Cele\csb-1
1 of 14
No
No
Cele\hpl-1
1 of 14
No
No
Cele\hpl-2
1 of 14
No
No
Cele\isw-1
1 of 14
No
No
Cele\M03C11.8
1 of 14
No
No
Cele\phf-32
1 of 14
No
No
Cele\phf-33
1 of 14
No
Yes
Cele\pqn-15
1 of 14
No
No
Cele\rad-26
1 of 14
No
No
Cele\rad-54.L
1 of 14
No
No
Cele\smrc-1
1 of 14
No
No
Cele\swsn-4
1 of 14
No
No
Cele\xnp-1
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (18)
Agam\AgaP_AGAP012009
12 of 12
Yes
Yes
Agam\AgaP_AGAP001820
1 of 12
No
No
Agam\AgaP_AGAP002490
1 of 12
No
No
Agam\AgaP_AGAP003597
1 of 12
No
No
Agam\AgaP_AGAP004723
1 of 12
No
No
Agam\AgaP_AGAP006945
1 of 12
No
No
Agam\AgaP_AGAP007573
1 of 12
No
No
Agam\AgaP_AGAP008426
1 of 12
No
No
Agam\AgaP_AGAP008482
1 of 12
No
No
Agam\AgaP_AGAP008698
1 of 12
No
No
Agam\AgaP_AGAP008748
1 of 12
No
No
Agam\AgaP_AGAP009344
1 of 12
No
No
Agam\AgaP_AGAP009444
1 of 12
No
No
Agam\AgaP_AGAP009980
1 of 12
No
No
Agam\AgaP_AGAP010462
1 of 12
No
No
Agam\AgaP_AGAP010699
1 of 12
No
No
Agam\AgaP_AGAP010700
1 of 12
No
No
Agam\AgaP_AGAP012013
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (33)
Atha\PKL
11 of 13
Yes
Yes
Atha\PKR2
11 of 13
Yes
Yes
Atha\CHR4
7 of 13
No
Yes
Atha\BRM
2 of 13
No
No
Atha\SYD
2 of 13
No
No
Atha\ASG3
1 of 13
No
No
Atha\AT3G31900
1 of 13
No
No
Atha\AT3G32280
1 of 13
No
No
Atha\AT3G32330
1 of 13
No
No
Atha\AT5G07810
1 of 13
No
No
Atha\AT5G43530
1 of 13
No
No
Atha\ATCHR12
1 of 13
No
No
Atha\ATRX
1 of 13
No
No
Atha\CHR1
1 of 13
No
No
Atha\CHR5
1 of 13
No
No
Atha\CHR8
1 of 13
No
Yes
Atha\CHR11
1 of 13
No
No
Atha\CHR17
1 of 13
No
No
Atha\CHR18
1 of 13
No
No
Atha\CHR23
1 of 13
No
No
Atha\CHR24
1 of 13
No
Yes
Atha\chr31
1 of 13
No
No
Atha\CHR34
1 of 13
No
No
Atha\CHR38
1 of 13
No
No
Atha\CHR40
1 of 13
No
No
Atha\CHR42
1 of 13
No
No
Atha\DRD1
1 of 13
No
No
Atha\ETL1
1 of 13
No
No
Atha\RAD5
1 of 13
No
No
Atha\RAD54
1 of 13
No
No
Atha\RGD3
1 of 13
No
No
Atha\SWI2
1 of 13
No
Yes
Atha\TFL2
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (12)
Scer\CHD1
2 of 13
Yes
No
Scer\IRC5
2 of 13
Yes
Yes
Scer\FUN30
1 of 13
No
No
Scer\INO80
1 of 13
No
No
Scer\ISW1
1 of 13
No
No
Scer\ISW2
1 of 13
No
No
Scer\MOT1
1 of 13
No
No
Scer\RAD26
1 of 13
No
Yes
Scer\RAD54
1 of 13
No
No
Scer\RDH54
1 of 13
No
No
Scer\SNF2
1 of 13
No
No
Scer\STH1
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (14)
Spom\mit1
4 of 12
Yes
Yes
Spom\hrp1
2 of 12
No
No
Spom\hrp3
2 of 12
No
No
Spom\chp2
1 of 12
No
No
Spom\clr4
1 of 12
No
No
Spom\fft1
1 of 12
No
No
Spom\fft2
1 of 12
No
No
Spom\fft3
1 of 12
No
No
Spom\rdh54
1 of 12
No
No
Spom\rhp26
1 of 12
No
Yes
Spom\rhp54
1 of 12
No
No
Spom\snf21
1 of 12
No
No
Spom\snf22
1 of 12
No
No
Spom\swi6
1 of 12
No
No
Escherichia coli (enterobacterium) (1)
Ecol\rapA
2 of 11
Yes
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Mi-2. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (25)
Chd3
9 of 13
Chd1
5 of 13
kis
5 of 13
brm
3 of 13
CG4049
3 of 13
Etl1
3 of 13
Hel89B
3 of 13
Iswi
3 of 13
lds
3 of 13
okr
3 of 13
XNP
3 of 13
CG10445
2 of 13
dom
2 of 13
Ino80
2 of 13
Marcal1
2 of 13
CG7376
1 of 13
CG7379
1 of 13
HP1b
1 of 13
HP1c
1 of 13
HP1e
1 of 13
Ing3
1 of 13
Lint-1
1 of 13
Pc
1 of 13
Su(var)205
1 of 13
tou
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 1 )
Human Ortholog
Disease
Evidence
References
CHD4; chromodomain helicase DNA binding protein 4
model of  Sifrim-Hitz-Weiss syndrome
IEA
(FlyBase, 2019-)
Modifiers Based on Experimental Evidence ( 8 )
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
CHD5; chromodomain helicase DNA binding protein 5
13 of 14
610771
    CHD3; chromodomain helicase DNA binding protein 3
    12 of 14
    602120
      CHD4; chromodomain helicase DNA binding protein 4
      12 of 14
      603277
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      Mi-2
      anti bait coimmunoprecipitation, western blot, pull down, autoradiography, Identification by mass spectrometry
      (Reddy et al., 2010, Stielow et al., 2008)
      Mi-2 - CDK2AP1
      anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry
      (Reddy et al., 2010)
      Mi-2 - CG2691
      experimental knowledge based
      (Guruharsha et al., 2011)
      Mi-2 - CG3726
      anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
      (Rhee et al., 2014)
      Mi-2 - CG8939
      experimental knowledge based
      (Guruharsha et al., 2011)
      Mi-2 - Caf1-55
      anti bait coimmunoprecipitation, Identification by mass spectrometry, anti tag coimmunoprecipitation, western blot, molecular sieving, molecular weight estimation by staining
      (Mugat et al., 2020, Zhang et al., 2016, Fasulo et al., 2012, Reddy et al., 2010)
      Mi-2 - CtBP
      anti bait coimmunoprecipitation, Identification by mass spectrometry
      (Reddy et al., 2010)
      Mi-2 - EcR
      anti bait coimmunoprecipitation, western blot, molecular weight estimation by staining, proximity-dependent biotin identification, Identification by mass spectrometry, pull down, autoradiography
      (Mazina et al., 2020, Brehm, 2017.5.22, Kreher et al., 2017)
      Mi-2 - HDAC1
      anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot, molecular sieving, molecular weight estimation by staining, anti tag coimmunoprecipitation
      (Mugat et al., 2020, Fasulo et al., 2012, Reddy et al., 2010, Kunert et al., 2009, Murawska et al., 2008)
      Mi-2 - His3
      anti bait coimmunoprecipitation, western blot
      (Demirdizen et al., 2019)
      Mi-2 - Lint-O
      anti bait coimmunoprecipitation, Identification by mass spectrometry
      (Reddy et al., 2010)
      Mi-2 - MBD-like
      ion exchange chromatography, molecular sieving, western blot, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation, Identification by mass spectrometry
      (Fasulo et al., 2012, Reddy et al., 2010, Ballestar et al., 2001)
      Mi-2 - MEP-1
      pull down, autoradiography, anti bait coimmunoprecipitation, western blot, ion exchange chromatography, affinity chromatography technology, molecular weight estimation by staining, molecular sieving, Identification by mass spectrometry, peptide massfingerprinting, anti tag coimmunoprecipitation
      (Mugat et al., 2020, Cugusi et al., 2015, Reddy et al., 2010, Kunert et al., 2009, Stielow et al., 2008)
      Mi-2 - MTA1-like
      anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot, molecular sieving, molecular weight estimation by staining, ion exchange chromatography
      (Mugat et al., 2020, Reddy et al., 2010, Ballestar et al., 2001)
      Mi-2 - S6kII
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Mi-2 - Sfmbt
      pull down, autoradiography
      (Stielow et al., 2008)
      Mi-2 - Srp72
      experimental knowledge based
      (Guruharsha et al., 2011)
      Mi-2 - Su(var)2-10
      anti tag coimmunoprecipitation, western blot
      (Mugat et al., 2020)
      Mi-2 - Sumo
      pull down, autoradiography
      (Stielow et al., 2008)
      Mi-2 - Top2
      anti bait coimmunoprecipitation, western blot
      (Cugusi et al., 2015)
      Mi-2 - Trl
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Nakayama et al., 2012)
      Mi-2 - Tsc1
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Mi-2 - arx
      pull down, Identification by mass spectrometry, luminescence based mammalian interactome mapping, anti tag coimmunoprecipitation, western blot
      (Mugat et al., 2020)
      Mi-2 - bon
      anti tag coimmunoprecipitation, anti tag western blot
      (Godneeva et al., 2023)
      Mi-2 - cid
      anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry, western blot
      (Demirdizen et al., 2019)
      Mi-2 - foxo
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Mi-2 - gig
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Mi-2 - kmg
      anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation
      (Kim et al., 2017)
      Mi-2 - l(1)G0020
      experimental knowledge based
      (Guruharsha et al., 2011)
      Mi-2 - mle
      anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot, anti bait coimmunoprecipitation
      (Cugusi et al., 2015)
      Mi-2 - nej
      anti bait coimmunoprecipitation, Identification by mass spectrometry
      (Reddy et al., 2010)
      Mi-2 - piwi
      anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry
      (Mugat et al., 2020, Onishi et al., 2020)
      Mi-2 - rictor
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Mi-2 - simj
      anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Cugusi et al., 2015, Fasulo et al., 2012, Reddy et al., 2010, Kunert et al., 2009, Kon et al., 2005)
      Mi-2 - su(Hw)
      anti bait coimmunoprecipitation, western blot
      (Vorobyeva et al., 2021)
      Mi-2 - ttk
      anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
      (Rhee et al., 2014, Reddy et al., 2010)
      Mi-2 - ush
      anti tag coimmunoprecipitation, western blot
      (Lenz et al., 2021)
      RNA-protein
      Physical Interaction
      Assay
      References
      Mi-2 - ERp60
      anti tag coimmunoprecipitation, quantitative reverse transcription pcr, iclip, partial RNA sequence identification
      (Ullah et al., 2022)
      Mi-2 - FASN1
      anti tag coimmunoprecipitation, quantitative reverse transcription pcr, iclip, partial RNA sequence identification
      (Ullah et al., 2022)
      Mi-2 - Hsp70
      anti tag coimmunoprecipitation, quantitative reverse transcription pcr, electrophoretic mobility shift assay, molecular weight estimation by staining
      (Murawska et al., 2011)
      Mi-2 - Hsp70Aa
      electrophoretic mobility shift assay, fluorescence technology, iclip, partial RNA sequence identification
      (Ullah et al., 2022)
      Mi-2 - Hsp83
      anti tag coimmunoprecipitation, quantitative reverse transcription pcr
      (Murawska et al., 2011)
      Mi-2 - LanA
      anti tag coimmunoprecipitation, quantitative reverse transcription pcr, iclip, partial RNA sequence identification
      (Ullah et al., 2022)
      Mi-2 - LanB1
      anti tag coimmunoprecipitation, quantitative reverse transcription pcr, iclip, partial RNA sequence identification
      (Ullah et al., 2022)
      Mi-2 - Pgant35A
      electrophoretic mobility shift assay, fluorescence technology, iclip, partial RNA sequence identification
      (Ullah et al., 2022)
      Mi-2 - alt
      anti tag coimmunoprecipitation, quantitative reverse transcription pcr, iclip, partial RNA sequence identification
      (Ullah et al., 2022)
      Mi-2 - kay
      iclip, partial RNA sequence identification, anti tag coimmunoprecipitation, quantitative reverse transcription pcr
      (Ullah et al., 2022)
      Mi-2 - pAbp
      iclip, partial RNA sequence identification, anti tag coimmunoprecipitation, quantitative reverse transcription pcr
      (Ullah et al., 2022)
      Mi-2 - sqd
      iclip, partial RNA sequence identification, anti tag coimmunoprecipitation, quantitative reverse transcription pcr
      (Ullah et al., 2022)
      Mi-2 - sty
      anti tag coimmunoprecipitation, quantitative reverse transcription pcr, iclip, partial RNA sequence identification
      (Ullah et al., 2022)
      Summary of Genetic Interactions
      Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Mi-2
      enhanceable
      Rcc1
      (Nikalayevich and Ohkura, 2015)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Atg1
      suppressible
      Mi-2
      (Umargamwala et al., 2025)
      dom
      enhanceable
      Mi-2
      (Ellis et al., 2015)
      E2f1
      enhanceable
      Mi-2
      (Ji et al., 2012)
      E2f1
      suppressible
      Mi-2
      (Ji et al., 2012)
      E(z)
      suppressible
      Mi-2
      (Bajusz et al., 2001)
      Egfr
      enhanceable
      Mi-2
      (Read et al., 2005)
      HDAC1|ttk
      enhanceable
      Mi-2
      (Murawsky et al., 2001)
      Kr
      enhanceable
      Mi-2
      (Abrell et al., 2000, Carrera et al., 1998)
      Nipped-B
      suppressible
      Mi-2
      (Fasulo et al., 2012)
      Pc
      enhanceable
      Mi-2
      (Kehle et al., 1998)
      Ras85D
      enhanceable
      Mi-2
      (Read et al., 2005)
      Rcc1
      enhanceable
      Mi-2
      (Nikalayevich and Ohkura, 2015)
      Ret
      enhanceable
      Mi-2
      (Read et al., 2005)
      ttk
      enhanceable
      Mi-2
      (Murawsky et al., 2001)
      wg
      enhanceable
      Mi-2
      (Kon et al., 2005)
      External Data
      Subunit Structure (UniProtKB)
      Interacts with the NuRD complex member HDAC1/Rpd3.
      (UniProt, O97159 )
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyBase
      External Links
      External Data
      Linkouts
      KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map
      3-46
      Cytogenetic map
      76D3-76D4
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      76D3-76D4
      Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338)
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      76D-76D
      (Müller, 1999.1.12)
      76D3-76D4
      (determined by in situ hybridisation)
      (Spradling et al., 1999)
      76D-76D
      (determined by in situ hybridisation)
      (Kehle et al., 1998, Szeged Stock Center, 1998-, Deak et al., 1997)
      76D1-76D2
      (determined by in situ hybridisation)
      (Kim et al., 1997)
      76D3-76D4
      (BDGP Project Members, 1994-1999)
      Experimentally Determined Recombination Data
      Location

      3-46

      (FlyBase, 2016)

      3-43.3

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (36)
      Genomic Clones (21)
      cDNA Clones (86)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Mazina et al., 2018, Mathieu et al., 2012, Reddy et al., 2010, Murawska et al., 2008)
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
          Other Comments

          DNA-protein interactions: genome-wide binding profile assayed for Mi-2 protein in S2 cells; GEO accession number GSE32404.

          (Moshkin et al., 2012)

          dsRNA has been made from templates generated with primers directed against this gene. RNAi of Mi-2 results in reduced arborization of ddaD and ddaE neurons, defects in muscle and defects in dendrite morphogenesis.

          (Parrish et al., 2006)

          ATPase activity, nucleosome binding and nucleosome mobilization activities are all modulated by phosphorylation of Mi-2.

          (Bouazoune and Brehm, 2005)

          The Mi-2 chromodomains are DNA binding modules.

          (Bouazoune et al., 2002)

          The Mi-2 chromodomains are important for ATP-dependent nucleosome mobilisation.

          (Bouazoune et al., 2002)

          Su(Tpl) is located in the largest intron of Mi-2, on the same strand. Overall orientation not stated: Mi-2+ Su(Tpl)+ Mi-2+

          (Khattak et al., 2002)

          It is proposed that Mi-2 defines a novel family of chromatin remodelling complex that is mechanistically distinct from both Iswi and SWI/SNF.

          (Brehm et al., 2000)

          GenBank AF007780; AAB87384 and SPTREMBL:016102 are chimeric between Chd3 and Mi-2, which are distinct genes.

          (Kennison and Müller, 2000.4.25)

          Isolated as a protein that interacts with hb protein in a yeast two-hybrid assay.

          (Kehle et al., 1998)
          Relationship to Other Genes
          Source for database merge of

          Source for merge of: Mi-2 l(3)L1243

          (Cooper et al., 2010)
          Additional comments
          Nomenclature History
          Source for database identify of
          Nomenclature comments
          Etymology

          Named 'Mi-2' after the human ortholog.

          (FlyBase, 2009-)
          Synonyms and Secondary IDs (29)
          Reported As
          Symbol Synonym
          0006/06
          (Deak et al., 1997)
          0854/01
          (Deak et al., 1997)
          CG8103
          (Ashraf et al., 2020, Christis and Munro, 2012, Ni et al., 2011.7.19, Ying et al., 2011, Ni et al., 2010.12.1, Reddy et al., 2010, Keleman et al., 2009.8.5, Schneiderman et al., 2009, Stielow et al., 2008, Quinones-Coello, 2007, Bienz, 2006, Molnar et al., 2006, Fouix et al., 2003, Whitfield, 2000.4.27)
          Chd4
          (Marfella and Imbalzano, 2007)
          Dm-Mi-2
          (Lee et al., 1999)
          M-i2
          (Feller et al., 2012)
          MI-2
          (Ho et al., 2014, Mills, 2010, Marhold et al., 2004)
          MI2
          (Moshkin et al., 2012, Schwartz and Pirrotta, 2007, Sanjuan and Marin, 2001)
          Mi 2
          (Lasko, 2000)
          Mi-2
          (Choudhury et al., 2024, Guo et al., 2024, Aughey et al., 2023, Godneeva et al., 2023, Yheskel et al., 2023, Beaver et al., 2022, McCarthy et al., 2022, Repton et al., 2022, Richards et al., 2022, Bilder et al., 2021, Onishi et al., 2021, Parkhitko et al., 2021, Takakura et al., 2021, Vidaurre and Chen, 2021, Vorobyeva et al., 2021, Ashraf et al., 2020, Gamez et al., 2020, Jin et al., 2020, Mazina et al., 2020, Mugat et al., 2020, Onishi et al., 2020, Angulo et al., 2019, Demirdizen et al., 2019, Jiang et al., 2019, Simon et al., 2019, Kreher et al., 2017, Lou et al., 2017, Mazina et al., 2017, Transgenic RNAi Project members, 2017-, Dorsett, 2016, Cugusi et al., 2015, Dietz et al., 2015, Ellis et al., 2015, Gene Disruption Project members, 2015-, Nikalayevich and Ohkura, 2015, Schertel et al., 2015, Rogers et al., 2014, de Celis et al., 2013.9.11, Kwon et al., 2013, Lindsley et al., 2013, Merzetti et al., 2013, Antao et al., 2012, Holmqvist et al., 2012, Ji et al., 2012, Ambegaokar and Jackson, 2011, Davis et al., 2011, Du et al., 2011, Friedman et al., 2011, Kirilly et al., 2011, Cooper et al., 2010, Gan et al., 2010, Kallappagoudar et al., 2010, Knowles-Barley et al., 2010, Smulders-Srinivasan et al., 2010, Wasbrough et al., 2010, Clapier and Cairns, 2009, Nie et al., 2009, Schneiderman et al., 2009, van Vugt et al., 2009, Bajpe et al., 2008, Doheny et al., 2008, Levine and Begun, 2008, Stielow et al., 2008, Buszczak et al., 2007, Quinones-Coello, 2007, Bienz, 2006, Bouazoune and Brehm, 2006, Molnar et al., 2006, Bouazoune and Brehm, 2005)
          Mi2
          (Godneeva et al., 2023, Halim et al., 2020, Doyen et al., 2015, De Vaux et al., 2013, Schuettengruber et al., 2011, Reddy et al., 2010, Mendjan et al., 2006, Parrish et al., 2006, Varga-Weisz and Becker, 2006, Mason et al., 2004)
          Pha
          (Bloomington Drosophila Stock Center, 1999.2.23, Spradling et al., 1999, BDGP Project Members, 1994-1999)
          dMI-2
          (Demakov et al., 2011, Wang and Brock, 2003, Halbach et al., 2000)
          dMi
          (Bajusz et al., 2001)
          dMi-2
          (Ullah et al., 2022, Vaid et al., 2020, Kovač et al., 2018, Kim et al., 2017, Tue et al., 2017, Comoglio and Paro, 2014, Koe et al., 2014, Zhimulev et al., 2014, Li and Zhou, 2013, Fasulo et al., 2012, Nakayama et al., 2012, Kharchenko et al., 2011, Murawska et al., 2011, Li et al., 2010, Kunert et al., 2009, Murawska et al., 2008, Smith et al., 2008, Polo and Almouzni, 2006, Yamasaki and Nishida, 2006, Kon et al., 2005, Read et al., 2005, Brehm et al., 2004, Levine et al., 2004, Clyde and Small, 2003, Fouix et al., 2003, Lusser and Kadonaga, 2003, Perrin et al., 2003, Bouazoune et al., 2002, Hirose et al., 2002, Khattak et al., 2002, Neely and Workman, 2002, Ballestar et al., 2001, Barlow et al., 2001, Eissenberg, 2001, Murawsky et al., 2001, Saurin et al., 2001, Zbylut et al., 2001, Abrell et al., 2000, Ahringer, 2000, Brehm et al., 2000, Farkas et al., 2000, Aasland, 1999, Aasland, 1999, Fritsch et al., 1999, Miska, 1999, Müller, 1999.1.12, Müller, 1999.12.15, Tweedie et al., 1999, van Lohuizen, 1999, Wade and Wolffe, 1999, Kehle et al., 1998)
          dMi2
          (Rennie et al., 2018, Sakoparnig et al., 2012, Thompson and Travers, 2008)
          hip76
          (Kehle et al., 1998)
          l(3)01058
          l(3)76BDe
          (Cooper et al., 2010)
          l(3)A154.3M3
          (Carrera et al., 1998)
          l(3)L1243
          (Ambegaokar and Jackson, 2011)
          l(3)S000606
          (Szeged Stock Center, 1998-)
          l(3)S085401
          (Szeged Stock Center, 1998-)
          l(3)j3D4
          pha
          (Kim et al., 1997)
          Name Synonyms
          Mi-2
          Mi-2 ortholog
          PHD-containing ATPase
          (Kim et al., 1997)
          dMi-2
          (Comoglio and Paro, 2014)
          hb-interacting protein 76
          (Kehle et al., 1998)
          lethal (3) L1243
          Secondary FlyBase IDs
          • FBgn0013591
          • FBgn0010735
          • FBgn0011790
          • FBgn0028080
          • FBgn0028245
          • FBgn0024536
          Datasets (2)
          Study focus (2)
          Experimental Role
          Project
          Project Type
          Title
          • bait_protein
          modENCODE_chromatin_proteins_cells
          Genome-wide localization of chromosomal proteins in cell lines by ChIP-chip and ChIP-Seq.
          • bait_protein
          modENCODE_chromatin_proteins_flies
          Genome-wide localization of chromosomal proteins in fly tissues by ChIP-chip and ChIP-Seq.
          Study result (0)
          Result
          Result Type
          Title
          External Crossreferences and Linkouts ( 80 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          Linkouts
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          MIST (genetic) - An integrated Molecular Interaction Database
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          References (256)