FlyBase Gene Report: Dmel\eIF4H1

General Information

Symbol

Dmel\eIF4H1

Species

D. melanogaster

Name

eukaryotic translation initiation factor 4H1

Annotation Symbol

CG4429

Feature Type

protein_coding_gene

FlyBase ID

FBgn0262734

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Rbp2, MRE9, RRM2, eIF4H, RNA-binding protein 2

Key Links

Genomic Location

Cytogenetic map

14C6-14C8

Sequence location

X:16,448,665..16,454,972 [+]

Recombination map

1-53

RefSeq locus

NC_004354 REGION:16448665..16454972

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables mRNA binding

inferred from sequence or structural similarity

(Lasko, 2000)

enables ribosomal small subunit binding

inferred from biological aspect of ancestor with PANTHER:PTN000579859

(assigned by GO_Central )

(Gaudet et al., 2011)

enables RNA strand annealing activity

inferred from biological aspect of ancestor with PANTHER:PTN000579859

(assigned by GO_Central )

(Gaudet et al., 2011)

enables RNA strand-exchange activity

inferred from biological aspect of ancestor with PANTHER:PTN000579859

(assigned by GO_Central )

(Gaudet et al., 2011)

enables translation initiation factor activity

inferred from sequence or structural similarity

(Lasko, 2000)

Biological Process (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in eukaryotic translation initiation factor 4F complex assembly

inferred from biological aspect of ancestor with PANTHER:PTN000579859

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in formation of translation preinitiation complex

inferred from biological aspect of ancestor with PANTHER:PTN000579859

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in translational initiation

inferred from sequence or structural similarity

(Lasko, 2000)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in cytosol

inferred from sequence or structural similarity

(Lasko, 2000)

Gene Group (FlyBase)

CYTOPLASMIC TRANSLATION INITIATION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CYTOPLASMIC TRANSLATION INITIATION FACTORS -: Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\eIF4H1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry X2JC79)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

eIF4H1-RA

FBtr0074279

NM_080220

3898

325

eIF4H1-RB

FBtr0074280

NM_167522

3895

324

eIF4H1-RC

FBtr0074281

NM_206764

2640

358

eIF4H1-RD

FBtr0343632

NM_001298401

2730

388

eIF4H1-RE

FBtr0343633

NM_001298402

2816

317

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

eIF4H1-PA

FBpp0074055

35.1

325

9.83

FBpp0074056

34.9

324

9.68

FBpp0089381

38.3

358

9.83

FBpp0310225

41.8

388

10.26

FBpp0310226

34.2

317

9.73

X2JE34

NP_001285331

AHN59801

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF4H1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rbp2_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\eIF4H1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\eIF4H1

View exonic expression by tissue for Dmel\eIF4H1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

49809/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0262734

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010256

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0262734

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of eIF4H1

Transgenic constructs containing regulatory region of eIF4H1

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(1)FDD-0024486

(Marygold et al., 2007)

Df(1)FDD-0230908

(Marygold et al., 2007)

Gene duplicated in

Dp(1;1)FDDP-0024486

(Cook, 2014.8.18)

Dp(1;3)DC134

(Popodi et al., 2010-, Venken et al., 2010)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^da.G32

eIF4H1^HMS04504

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (28)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\EIF4H

13 of 15

Yes

Hsap\EIF4B

3 of 15

Hsap\BOLL

1 of 15

Hsap\CIRBP

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (30)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Eif4h

12 of 15

Yes

Mmus\Eif4b

3 of 15

Mmus\Boll

1 of 15

Mmus\Cirbp

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Rattus norvegicus (Norway rat) (26)

Rnor\Eif4h

12 of 13

Yes

Rnor\Eif4b

2 of 13

Rnor\Boll

1 of 13

Rnor\Cirbp

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (22)

Xtro\eif4h

11 of 12

Yes

Xtro\eif4b

3 of 12

Xtro\cirbp

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Danio rerio (Zebrafish) (29)

Drer\eif4h

12 of 15

Yes

Drer\eif4ba

3 of 15

Drer\eif4bb

3 of 15

Drer\cirbpa

1 of 15

Yes

Drer\cirbpb

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\zgc:55733

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (9)

Cele\drr-2

5 of 15

Yes

Cele\daz-1

1 of 15

Cele\H05C05.1

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\AT3G47120

2 of 9

Yes

Atha\NUC-L1

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (15)

Scer\TIF3

3 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Scer\MUD1

1 of 15

Scer\NAB3

1 of 15

Scer\NAB6

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\sce3

6 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (26)

Gene Symbol

Score

Source

Alignment

6 of 10

5 of 10

3 of 10

2 of 10

bol

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

ssx

1 of 10

Sxl

1 of 10

tra2

1 of 10

trv

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

eIF4H1^KK108805

ameliorates fragile X-associated tremor/ataxia syndrome

modeled by Zzzz\CGG^90.UAS.EGFP

(Linsalata et al., 2019)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis-1

modeled by Zzzz\GGGGCC^{44.LDS.UAS.GR-GFP}

(Goodman et al., 2019)

eIF4H1^HMS04504

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis-1

modeled by Zzzz\GGGGCC^{44.LDS.UAS.GR-GFP}

(Goodman et al., 2019)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EIF4H; eukaryotic translation initiation factor 4H

13 of 15

603431

EIF4B; eukaryotic translation initiation factor 4B

3 of 15

603928

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262734

MIST (protein-protein) - An integrated Molecular Interaction Database

Rbp2

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0262734

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-53

Cytogenetic map

14C6-14C8

Sequence location

X:16,448,665..16,454,972 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

14C6-14C8

Limits computationally determined from genome sequence between P{EP}EP1547^EP1547 and P{EP}CG4928^EP1341

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-53

(FlyBase, 2016)

1-54.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

15830

y¹ w^67c23 P{EPgy2}eIF4H1^EY00852

83490

y¹ sc^* v¹ sev²¹; P{TKO.GS04195}attP40

43275

y¹ sc^* v¹ sev²¹; P{TRiP.GLC01464}attP2

57306

y¹ sc^* v¹ sev²¹; P{TRiP.HMS04504}attP40

FlyORF

F002824

M{UAS-eIF4H1.ORF.3xHA.GW}ZH-86Fb

VDRC

v100817

P{KK108805}VIE-260B

v34301

w¹¹¹⁸; P{GD10708}v34301

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (166)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI04456

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0262734

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

49809

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: eIF4H1 Rbp2

(Marygold et al., 2017)

Source for database merge of

Source for merge of: Rbp2 anon- EST:fe1B9

(FlyBase, 1996-)

Source for merge of: Rbp2 CG4429

(Misra, 2000.7.28)

Source for merge of: Rbp2 MRE9

(FlyBase Genome Annotators, 2011)

Additional comments

MRE9 is part of the 3' UTR of Rbp2.

(FlyBase Genome Annotators, 2011)

MRE9 corresponds to a portion of the 3' UTR of Rbp2.

(FlyBase Genome Annotators, 2011)

Other Comments

A sequence comparison and numerical analysis of the RRM-containing (RNA recognition motif) proteins suggests that functionally related RRM-containing proteins have significant sequence similarities in their RRMs.

(Kim and Baker, 1993)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

49809

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

X2JC79

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

X2JC79

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0262734

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

49809/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0262734

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rbp2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010256

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0262734

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0262734

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

49809

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4429

MARRVEL_MODEL - MARRVEL (model organism gene)

49809

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262734

MIST (protein-protein) - An integrated Molecular Interaction Database

Rbp2

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG4429

(Kolkhof et al., 2017, Marygold et al., 2017, Horan et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Hughes et al., 2008, Lasko, 2000)

EIF4H1

(Linsalata et al., 2019)

MRE9

(FlyBase Genome Annotators, 2011, Kageyama, 2007.2.28, Inagaki et al., 2005)

RNA-binding protein 2

(Hosamani et al., 2016)

RRM2

(Gan et al., 2014, Kim, 1993.3.10)

Rbp2

(Sapiro et al., 2020, Transgenic RNAi Project members, 2017-, Horan et al., 2015, Kwon et al., 2013, FlyBase Genome Annotators, 2011, Friedman et al., 2011, Hartmann et al., 2011, Parker et al., 2011, Popodi et al., 2010-, Venken et al., 2010, Lindsay et al., 2008, Akdemir et al., 2007, Maines et al., 2007, Marygold et al., 2007, Song and Tanouye, 2007)

anon-EST:fe1B9

cg4429

(Lasko, 2000)

eIF4H

(Liguori et al., 2021, Horan et al., 2015, Lasko, 2000)

eIF4H1

(Sapiro et al., 2020, Goodman et al., 2019, Linsalata et al., 2019, Marygold et al., 2017)

rrm2

(Kim and Baker, 1993)

Name Synonyms

RNA-binding protein 2

anon-fast-evolving-1B9

eukaryotic translation initiation factor 4H1

mRNA-like ncRNA in embryogenesis 9

Secondary FlyBase IDs

FBgn0010256
FBgn0025264
FBgn0030754
FBgn0086541

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (56)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections