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General Information
Symbol
Dmel\Gfrl
Species
D. melanogaster
Name
Glial cell line-derived neurotrophic family receptor-like
Annotation Symbol
CG43224
Feature Type
FlyBase ID
FBgn0262869
Gene Model Status
Stock Availability
Gene Summary
a GPI-anchored membrane protein with four GDNF family receptor-like domains - regulates oogenesis and male fertility - expressed in the central and peripheral nervous systems (Interactive Fly, Gfrl )
Contribute a Gene Snapshot for this gene.
Also Known As

mun, munin

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
3-68
RefSeq locus
NT_033777 REGION:20378458..20484588
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001306067
(assigned by GO_Central )
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001306067
(assigned by GO_Central )
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001306067
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001306067
(assigned by GO_Central )
Gene Group (FlyBase)
Protein Family (UniProt)
-
Summaries
Summary (Interactive Fly)

a GPI-anchored membrane protein with four GDNF family receptor-like domains - regulates oogenesis and male fertility - expressed in the central and peripheral nervous systems

Gene Model and Products
Number of Transcripts
10
Number of Unique Polypeptides
8

Please see the JBrowse view of Dmel\Gfrl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.44

Stop-codon suppression (UGA) postulated; FBrf0216884.

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0306199
6598
1279
FBtr0306200
6524
864
FBtr0306201
7568
1212
FBtr0306202
7235
1101
FBtr0306203
6279
1168
FBtr0306204
6630
1212
FBtr0306205
3139
214
FBtr0330033
7568
1217
FBtr0340192
5901
1042
FBtr0346115
8106
1212
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0297308
140.4
1279
6.60
FBpp0297309
95.6
864
7.42
FBpp0297310
133.4
1212
6.62
FBpp0297311
121.8
1101
6.69
FBpp0297312
128.8
1168
6.67
FBpp0297313
133.4
1212
6.62
FBpp0297314
24.3
214
8.35
FBpp0303066
133.9
1217
6.63
FBpp0309169
114.2
1042
7.15
FBpp0311944
133.4
1212
6.62
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1212 aa isoforms: Gfrl-PG, Gfrl-PJ, Gfrl-PN
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gfrl using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: Transcript of 7000nt with stronger expression than 7500nt

organism

Comment: Similar expression level for transcripts of 7000 and 7500nt.

organism

Comment: Similar expression level for transcripts of 7000 and 7500nt.

RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: Assay specific to exons 2-16 identified transcript B (2.6kb) and Bb (2.3kb)

organism

Comment: Probe corresponding to exons 15-23 identified one major ~1kb band

organism

Comment: Assay specific to exons 2-16 identified transcript B (2.6kb) and Bb (2.3kb)

organism

Comment: Assay specific to exons 2-16 identified transcript B (2.6kb) and Bb (2.3kb)

Additional Descriptive Data

Gfrl transcript is detected at stage 13 in the ventral neuroectoderm of seven abdominal segments, in single or small clusters of neurons located laterally or on both sides of the midline, and in cell clusters close to the oesophagus. In later embryonic stages expression is observed in all segments of the ventral nerve cord and in single neurons or neuron clusters located more laterally, throughout the peripheral sensory nervous system and in the dorsal vessel. In third instar larva, Gfrl transcript is observed in symmetric regions of the posterior brain lobes. In the adult, expression is mostly detected in the brain cortex.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
Reporter: P{GMR93G06-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{GMR93G08-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{GMR93G10-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{GMR93G11-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{GMR93G12-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{GMR93H01-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{GMR95B08-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Gfrl in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 13 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Gfrl
Transgenic constructs containing regulatory region of Gfrl
Aberrations (Deficiencies and Duplications) ( 3 )
Inferred from experimentation ( 3 )
Gene partially disrupted in
Gene disrupted in
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
6 of 15
Yes
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
6 of 15
Yes
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
Rattus norvegicus (Norway rat) (5)
5 of 13
Yes
Yes
3 of 13
No
Yes
3 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (4)
3 of 12
Yes
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
Danio rerio (Zebrafish) (8)
4 of 15
Yes
Yes
4 of 15
Yes
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (5)
7 of 15
Yes
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091901BB )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500R7 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0AZT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0AWX )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G00W2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-68
    Cytogenetic map
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    92E8-92E8
    Limits computationally determined from genome sequence between P{PZ}l(3)1058510585 and P{EP}SyndEP409
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (13)
    Genomic Clones (94)
    cDNA Clones (30)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
      RNAi and Array Information
      Linkouts
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: mun CG17204

      Source for identity of: Gfrl mun

      Source for database merge of

      Source for merge of: CG34118 CG17208

      Source for merge of: CG17205 BcDNA:GH26447

      Source for merge of: mun CG4342

      Source for merge of: CG34119 mun

      Source for merge of: mun CG17203

      Source for merge of: CG42801 mun

      Additional comments

      Annotations CG42801 and CG42625 merged as CG43224 in release 5.34 of the genome annotation.

      Annotations CG34118 and CG17208 merged as CG42801 in release 5.30 of the genome annotation.

      Annotations CG34119 and CG34352 merged as CG42625 in release 5.21 of the genome annotation.

      Annotations mun and CG4342 merged as CG42401 in release 5.10 of the genome annotation.

      Source for merge of CG17205 BcDNA:GH26447 was a shared cDNA ( date:030728 ).

      Annotation CG17205 split into CG34118 and CG34119 in release 5.1 of the genome annotation.

      Annotations CG17203 and CG17204 merged as CG33518 (which corresponds to mun) in release 4.1 of the genome annotation.

      Other Comments
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 71 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL - MARRVEL (model organism gene)
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (21)
      Reported As
      Name Synonyms
      CG4342
      GDNF Receptor-like
      Glial cell line-derived neurotrophic family receptor-like
      Secondary FlyBase IDs
      • FBgn0261342
      • FBgn0261970
      • FBgn0083954
      • FBan0017205
      • FBgn0038794
      • FBgn0064953
      • FBgn0040573
      • FBgn0083955
      • FBpp0083305
      • FBtr0083897
      • FBpp0083304
      • FBtr0083896
      • FBgn0038793
      • FBgn0038792
      • FBgn0038791
      • FBgn0053518
      • FBgn0259820
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (51)