FlyBase Gene Report: Dmel\cnc

General Information

Symbol

Dmel\cnc

Species

D. melanogaster

Name

cap-n-collar

Annotation Symbol

CG43286

Feature Type

protein_coding_gene

FlyBase ID

FBgn0262975

Gene Model Status

Stock Availability

23 publicly available

Gene Snapshot

cap-n-collar (cnc) encodes cap-n-collar (cnc) encodes a transcription factor that interacts with the product of Keap1 to regulate the activation of genes by oxidative stress. The cnc-encoded product also contributes to mRNA localization mediated by microtubules, dendrite morphogenesis and intestinal stem cell homeostasis. [Date last reviewed: 2019-03-07]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Nrf2, CncC, Cap 'n' collar, cap and collar, l(3)03921

Key Links

Genomic Location

Cytogenetic map

Sequence location

3R:23,185,580..23,226,711 [-]

Recombination map

3-78

RefSeq locus

NT_033777 REGION:23185580..23226711

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (21 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

contributes_to DNA-binding transcription factor activity

inferred from direct assay

(Veraksa et al., 2000)

enables protein heterodimerization activity

inferred from physical interaction with FLYBASE:maf-S; FB:FBgn0034534

(Veraksa et al., 2000)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN002399572

(assigned by GO_Central )

(Gaudet et al., 2011)

enables DNA-binding transcription factor activity

traceable author statement

(Guichet et al., 2001)

enables RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN002399572

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (15 terms)

Terms Based on Experimental Evidence (14 terms)

CV Term

Evidence

References

involved_in blastoderm segmentation

inferred from mutant phenotype

(Veraksa et al., 2000)

involved_in dendrite morphogenesis

inferred from mutant phenotype

(Iyer et al., 2013)

involved_in determination of adult lifespan

inferred from mutant phenotype

(Sykiotis and Bohmann, 2008)

involved_in head development

inferred from mutant phenotype

(Veraksa et al., 2000)

involved_in intestinal stem cell homeostasis

inferred from mutant phenotype

(Wang et al., 2014)

involved_in oocyte dorsal/ventral axis specification

inferred from mutant phenotype

(Guichet et al., 2001)

involved_in oocyte microtubule cytoskeleton polarization

inferred from mutant phenotype

(Guichet et al., 2001)

involved_in oocyte nucleus localization involved in oocyte dorsal/ventral axis specification

inferred from mutant phenotype

(Guichet et al., 2001)

involved_in pharynx development

inferred from mutant phenotype

(Veraksa et al., 2000)

involved_in positive regulation of transcription by RNA polymerase II

inferred from direct assay

(Veraksa et al., 2000)

involved_in regulation of bicoid mRNA localization

inferred from mutant phenotype

(Guichet et al., 2001)

involved_in regulation of pole plasm oskar mRNA localization

inferred from mutant phenotype

(Guichet et al., 2001)

involved_in response to endoplasmic reticulum stress

inferred from mutant phenotype

(Wang et al., 2014)

involved_in response to oxidative stress

inferred from mutant phenotype

(Sykiotis and Bohmann, 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN002399572

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Deng and Kerppola, 2013, Guichet et al., 2001, Veraksa et al., 2000)

located_in polytene chromosome puff

inferred from direct assay

(Deng and Kerppola, 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002399572

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

BASIC LEUCINE ZIPPER TRANSCRIPTION FACTORS

Protein Family (UniProt)

Belongs to the bZIP family. CNC subfamily. (P20482)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

BASIC LEUCINE ZIPPER TRANSCRIPTION FACTORS -: The basic leucine zipper (bZIP) transcription factors are sequence-specific DNA-binding proteins that regulate transcription. They are characterized by a 60-80 amino acid bZIP domain: a basic DNA binding domain followed by a leucine zipper dimerization domain. (Adapted from FBrf0152056).

Protein Function (UniProtKB)

Plays a role in posterior cephalic patterning. Probable subunit of a heterodimeric regulatory protein involved in the control of head morphogenesis. Isoform B may have a repressive effect on Dfd response elements, thereby modifying the activity and specificity of the Hox system and moving the body anterior/posterior axis.

(UniProt, P20482)

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

16

Number of Unique Polypeptides

7

Please see the JBrowse view of Dmel\cnc for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

gene_with_stop_codon_read_through ; SO:0000697

Stop-codon suppression (UGA) postulated; FBrf0216884.

Gene model reviewed during 5.44

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0306747

3477

533

FBtr0306749

5180

805

FBtr0306748

6701

1383

FBtr0306746

3570

533

FBtr0306745

3550

533

FBtr0306744

3575

533

FBtr0306750

3921

533

FBtr0306751

3552

533

FBtr0306752

4750

1133

FBtr0306753

6785

1383

FBtr0306754

6954

1383

FBtr0306755

5119

1046

FBtr0308226

4737

1133

FBtr0308227

4746

1133

FBtr0330372

6701

1430

FBtr0336455

4261

711

Additional Transcript Data and Comments

Reported size (kB)

6.6, 5.4, 3.3 (northern blot)

(McGinnis et al., 1998)

2.722 (longest cDNA)

(Mohler et al., 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0297670

56.5

533

9.06

FBpp0297672

85.2

805

7.66

FBpp0297671

147.4

1383

5.30

FBpp0297669

56.5

533

9.06

FBpp0297668

56.5

533

9.06

FBpp0297667

56.5

533

9.06

FBpp0297673

56.5

533

9.06

FBpp0297674

56.5

533

9.06

FBpp0297675

121.0

1133

6.35

FBpp0297676

147.4

1383

5.30

FBpp0297677

147.4

1383

5.30

FBpp0297678

111.6

1046

6.11

FBpp0300546

121.0

1133

6.35

FBpp0300547

121.0

1133

6.35

FBpp0303398

153.3

1430

5.49

FBpp0307561

75.5

711

7.17

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

533 aa isoforms: cnc-PA, cnc-PD, cnc-PE, cnc-PF, cnc-PG, cnc-PH

1383 aa isoforms: cnc-PC, cnc-PJ, cnc-PK

1133 aa isoforms: cnc-PI, cnc-PM, cnc-PN

Additional Polypeptide Data and Comments

Reported size (kDa)

1296, 805, 533 (aa)

(McGinnis et al., 1998)

533 (aa)

(Mohler et al., 1991)

Comments

cnc protein shows the strongest resemblance to the jun subclass of bZIP proteins in the basic binding domain but is very divergent from this class in the leucine-repeat region.

(Mohler et al., 1991)

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cnc using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

blastoderm stage

organism | 70% egg length

(Mohler et al., 1991)

embryonic stage

mandibular segment

(Mohler et al., 1991)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

intercalary segment

(Mohler et al., 1991)

blastoderm stage

organism | 90-100% egg length

Comment: 90-98% egg length

(Mohler et al., 1991)

embryonic stage 1 -- 4

organism | ubiquitous

Comment: reference states 0-2 hr AEL

(McGinnis et al., 1998)

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

clypeo-labral anlage in statu nascendi

(Fisher et al., 2012)

foregut anlage in statu nascendi

(Fisher et al., 2012)

ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

clypeo-labral anlage

(Fisher et al., 2012)

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

clypeo-labral primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

clypeo-labral primordium

(Fisher et al., 2012)

gnathal primordium

(Fisher et al., 2012)

labral sensory complex primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal head epidermis

(Fisher et al., 2012)

embryonic epipharynx

(Fisher et al., 2012)

embryonic hypopharynx

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

labral sensory complex

(Fisher et al., 2012)

embryonic stage 5 -- 12

(Mohler et al., 1991)

embryonic stage 7

organism | anterior

(Economou and Telford, 2009)

cephalic furrow | anterior to

(Economou and Telford, 2009)

embryonic stage 9

stomodeum | surrounding

(Economou and Telford, 2009)

embryonic stage 11

(Economou and Telford, 2009)

embryonic mandibular segment

(Economou and Telford, 2009)

hypopharyngeal lobe

(Economou and Telford, 2009)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14 -- 17

organism | ubiquitous

(McGinnis et al., 1998)

embryonic stage

embryonic labral segment

(McGinnis et al., 1998)

embryonic mandibular segment

(McGinnis et al., 1998)

(McGinnis et al., 1998)

embryonic stage 15 -- 17

organism | ubiquitous

(McGinnis et al., 1998)

Comment: reference states 12-24 hr AEL

(McGinnis et al., 1998)

embryonic stage 1 -- 4

Comment: reference states 0-2 hr AEL

(McGinnis et al., 1998)

embryonic stage 5 -- 17

Comment: reference states >2 hr AEL

(McGinnis et al., 1998)

Additional Descriptive Data

cnc is first seen in an anterior domain and a more posterior band anterior to the cephalic furrow at embryonic stage 7. By stage 9, expression extends from the posterior of the early "cap" of expression to form a ring around the stomodeum. In the germband extended embryo, there is a continuous expression domain from the mandibular lobes extending through the hypopharyngeal primordium and expression in the labrum.

(Economou and Telford, 2009)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 6 -- 17

embryonic mandibular segment

(McGinnis et al., 1998)

embryonic labral segment

(McGinnis et al., 1998)

embryonic stage 15 -- 17

organism | ubiquitous

(McGinnis et al., 1998)

embryonic stage 5

organism | ubiquitous

(McGinnis et al., 1998)

wandering third instar larval stage

wing pouch | restricted

Comment: excluded from D/V boundary

(Mohit et al., 2006)

presumptive capitellum | faint

(Mohit et al., 2006)

Additional Descriptive Data

cnc protein is expressed only in non-dorsal/ventral boundary cells of the wing pouch. No expression is observed in the haltere disc.

(Mohit et al., 2006)

Strong cnc staining is observed in the mandibular and labral segments from stage 6 on. This is presumed to be isoform B since only the mRNA for isoform B is expressed in these cells.

(McGinnis et al., 1998)

cnc antiserum detects a low level ubiquitous staining in syncytial embryos. From stage 6-14, staining is localized to the mandibular and labral segments. After stage 14 a low level ubiquitous staining is observed in addition to the strong staining in the mandibular and labral cells. The antibody recognizes all cnc isoforms.

(McGinnis et al., 1998)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Deng and Kerppola, 2013, Guichet et al., 2001, Veraksa et al., 2000)

located_in polytene chromosome puff

inferred from direct assay

(Deng and Kerppola, 2013)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\cnc in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\cnc

View exonic expression by tissue for Dmel\cnc

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0262975

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Images

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 50 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 21 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of cnc

Transgenic constructs containing regulatory region of cnc

Deletions and Duplications ( 11 )

Disrupted in

(Andrade et al., 2004.9.20)

(Christensen et al., 2008.9.29)

(Christensen et al., 2009.5.6)

(Mohler et al., 1995)

(Ryder and Roote, 2004.11.10)

(Ryder and Roote, 2004.11.10)

(Ryder, 2004.4.26)

(Ryder, 2004.4.26)

(Hales and Fuller, 1997)

Not disrupted in

(BDGP Project Members, 1994-1999)

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cnc^B.hs

lethal (with cnc^K6)

cnc^VL110

lethal (with cnc^VL110)

cnc^K6

lethal | heat sensitive

cnc^B.hs

lethal | recessive

lethal - all die before end of embryonic stage | recessive

partially lethal - majority die, with Scer\GAL4^pnr-MD237

cnc^GD4437

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

cnc^GD4437

viable, with Scer\GAL4^tin.CΔ4

cnc^GD4437

Sterility

Allele

cnc⁰³⁹²¹

cnc^PL00139

Other Phenotypes

Allele

developmental rate defective | larval stage, with Scer\GAL4⁵⁰¹⁵

cnc^dsRNA.C.UAS

developmental rate defective | larval stage, with Scer\GAL4^phm.PO

gravitaxis defective | adult stage, with Scer\GAL4^elav-C155

cnc^KK101639

increased mortality | adult stage | heat sensitive, with Scer\GAL4^elav-C155

cnc^KK101639

large body | pupal stage, with Scer\GAL4^phm.PO

cnc^dsRNA.C.UAS

locomotor behavior defective | adult stage, with Scer\GAL4^elav-C155

cnc^KK101639

neuroanatomy defective, with Scer\GAL4²²¹

neuroanatomy defective, with Scer\GAL4^ppk.1.9

neuroanatomy defective | third instar larval stage, with Scer\GAL4^ppk.PU

cnc^HMS02021

neurophysiology defective | third instar larval stage, with Scer\GAL4^elav-C155

cnc^UAS.cSa

neurophysiology defective | third instar larval stage | heat sensitive, with Scer\GAL4^elav-C155

cnc^KK101639

oxidative stress response defective, with Scer\GAL4^{αTub84B.Switch.PK}

sleep defective | adult stage, with Scer\GAL4^elav-C155

some die during embryonic stage | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

cnc^GD4437

visible, with Scer\GAL4^Bx-MS1096

cnc^{UAS.ORF.GW.Tag:HA}

visible, with Scer\GAL4^GMR.PF

cnc^UAS.cVa

visible, with Scer\GAL4^sev.EP

cnc^UAS.cSa

Phenotype manifest in

Allele

abdominal 1 ventral denticle belt | heat sensitive

cnc^B.hs

abdominal 2 ventral denticle belt | heat sensitive

cnc^B.hs

abdominal 3 ventral denticle belt | heat sensitive

cnc^B.hs

abdominal 4 ventral denticle belt | heat sensitive

cnc^B.hs

abdominal 5 ventral denticle belt | heat sensitive

cnc^B.hs

abdominal 6 ventral denticle belt | heat sensitive

cnc^B.hs

abdominal 7 ventral denticle belt | heat sensitive

cnc^B.hs

abdominal anterior ventral multidendritic neuron vdaa, with Scer\GAL4^ppk.1.9

abdominal ventral multidendritic neuron v'dap, with Scer\GAL4²²¹

cnc^HMS00650

cephalopharyngeal skeleton | larval stage

cnc^C.hs

cirri | ectopic

cirri | heat sensitive

cnc^B.hs

cirri | larval stage

dorsal appendage

cnc^C.hs

dorsal bridge | heat sensitive

cnc^B.hs

dorsal pouch | larval stage

egg chorion | dorsal | germline clone

egg chorion | maternal effect

cnc^unspecified

embryonic/larval pharynx | ectopic, with Scer\GAL4^69B

embryonic/larval pharynx | embryonic stage

cnc^VL110

embryonic intercalary segment

embryonic labral segment

embryonic mandibular segment

eye, with Scer\GAL4^GMR.PF

cnc^UAS.cVa

eye, with Scer\GAL4^sev.EP

cnc^UAS.cSa

cnc⁰³⁹²¹

hypostomal sclerite | heat sensitive

cnc^B.hs

cnc⁰³⁹²¹

labrum | larval stage

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

larval dorsal multidendritic neuron ddaE, with Scer\GAL4²²¹

larval head, with Scer\GAL4^69B

cnc^A.VP16.UAS

larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4^ppk.PU

cnc^HMS02021

lateral process

lateral process | anterior | heat sensitive

cnc^B.hs

lateral process | larval stage

maxillary segment, with Scer\GAL4^69B

cnc^A.VP16.UAS

mouth hook | ectopic

mouth hook | heat sensitive

cnc^B.hs

mouth hook | larval stage

mouth hook | supernumerary

ommatidium, with Scer\GAL4^GMR.PF

cnc^UAS.cVa

ommatidium, with Scer\GAL4^sev.EP

cnc^UAS.cSa

oocyte | germline clone

cnc⁰³⁹²¹

oocyte nucleus | germline clone

ovariole | germline clone

cnc⁰³⁹²¹

wing, with Scer\GAL4^Bx-MS1096

cnc^{UAS.ORF.GW.Tag:HA}

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

8 of 15

Yes

Yes

8 of 15

Yes

Yes

7 of 15

No

Yes

5 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

8 of 15

Yes

Yes

8 of 15

Yes

Yes

7 of 15

No

Yes

5 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

Yes

Rattus norvegicus (Norway rat) (6)

7 of 13

Yes

Yes

6 of 13

No

Yes

5 of 13

No

Yes

3 of 13

No

Yes

1 of 13

No

Yes

1 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (6)

9 of 12

Yes

Yes

6 of 12

No

Yes

4 of 12

No

Yes

3 of 12

No

Yes

1 of 12

No

Yes

1 of 12

No

Yes

Danio rerio (Zebrafish) (10)

9 of 15

Yes

Yes

7 of 15

No

Yes

7 of 15

No

Yes

7 of 15

No

Yes

5 of 15

No

Yes

5 of 15

No

Yes

2 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

6 of 15

Yes

Yes

5 of 15

No

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190387 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00009941

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501TH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY011566

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_03605

Mayetiola destructor

Hessian fly

Mdes\Mdes000839

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL015467

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL005077

Anopheles darlingi

American malaria mosquito

Adar\ADAC005491

Anopheles gambiae

Malaria mosquito

Agam\AGAP005300

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003645

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01LI )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001763

Bombyx mori

Silkmoth

Bmor\BGIBMGA001765

Heliconius melpomene

Postman butterfly

Hmel\HMEL014264

Apis florea

Little honeybee

Aflo\2894153988

Apis florea

Little honeybee

Aflo\2147575329

Apis mellifera

Western honey bee

Apis mellifera

Western honey bee

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001f00

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001efc

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001efe

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG015482

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG015484

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG015480

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM512570

Rhodnius prolixus

Kissing bug

Rpro\RPRC011620

Cimex lectularius

Bed bug

Clec\CLEC011277

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009066

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_05706

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01JN )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR009751

Strigamia maritima

European centipede

Smar\SMAR012788

Ixodes scapularis

Black-legged tick

Isca\ISCW019206

Stegodyphus mimosarum

African social velvet spider

Smim\KFM72135.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur07g04600

Tetranychus urticae

Two-spotted spider mite

Turt\tetur07g06850

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_120547

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_97492

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_104824

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_271125

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_271123

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_259722

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_259721

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_307821

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02EB )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000006014

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000003373

Ciona intestinalis

Vase tunicate

Cint\LOC101242152

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000015605

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000011937

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000015828

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 5 )

Allele

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Hsap\SNCA^UAS.cTa

(Barone et al., 2011)

ameliorates dilated cardiomyopathy 1A

modeled by LamC^G489V.UAS

(Bhide et al., 2018)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^Aβ42.lexAop

(Stapper and Jahn, 2018)

ameliorates dilated cardiomyopathy 1A

modeled by LamC^G489V.UAS

(Bhide et al., 2018)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^Aβ42.lexAop

(Stapper and Jahn, 2018)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NFE2L1; nuclear factor, erythroid 2 like 1

8 of 15

NFE2L2; nuclear factor, erythroid 2 like 2

8 of 15

IMMUNODEFICIENCY, DEVELOPMENTAL DELAY, AND HYPOHOMOCYSTEINEMIA; IMDDHH

NFE2; nuclear factor, erythroid 2

7 of 15

NFE2L3; nuclear factor, erythroid 2 like 3

5 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

iclip, partial RNA sequence identification

(Barckmann et al., 2015)

protein-protein

Physical Interaction

Assay

References

bimolecular fluorescence complementation, fluorescence microscopy

(Deng and Kerppola, 2014)

two hybrid array

(Shokri et al., 2019)

fluorescent resonance energy transfer

(Reinke et al., 2013)

fluorescent resonance energy transfer

(Reinke et al., 2013)

two hybrid array

(Shokri et al., 2019)

two hybrid array

(Shokri et al., 2019)

two hybrid array

(Shokri et al., 2019)

bimolecular fluorescence complementation, fluorescence microscopy, pull down, autoradiography

(Jain et al., 2015, Deng and Kerppola, 2014)

two hybrid array

(Shokri et al., 2019)

cnc - REPTOR-BP

fluorescent resonance energy transfer

(Reinke et al., 2013)

two hybrid array

(Shokri et al., 2019)

anti tag coimmunoprecipitation, western blot

(Chatterjee et al., 2016)

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Ntini and Wimmer, 2011)

electrophoretic mobility shift assay, autoradiography, fluorescent resonance energy transfer, two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Rahman et al., 2013, Reinke et al., 2013, Veraksa et al., 2000)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Veraksa et al., 2000)

suppressible

(Sykiotis and Bohmann, 2008)

suppressible

(Rahman et al., 2013)

enhanceable

(Rahman et al., 2013)

suppressible

(Deng and Kerppola, 2013)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Veraksa et al., 2000, Harding et al., 1995)

suppressible

(Wang et al., 2015)

enhanceable

(Sun et al., 2006)

suppressible

(Nagy et al., 2013)

suppressible

(Nagy et al., 2013)

suppressible

(Deng and Kerppola, 2013)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262975

MIST (genetic) - An integrated Molecular Interaction Database

cnc

MIST (protein-protein) - An integrated Molecular Interaction Database

cnc

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Factors involved in megakaryocyte development and platelet production

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3R

Recombination map

3-78

Cytogenetic map

Sequence location

3R:23,185,580..23,226,711 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

94E4-94E7

Limits computationally determined from genome sequence between P{EP}hh^EP3521 and P{PZ}cnc⁰³⁹²¹; Limits computationally determined from genome sequence between P{PZ}cnc⁰³⁹²¹ and P{PZ}l(3)06906⁰⁶⁹⁰⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

94E3-94E7

(determined by in situ hybridisation)

(Spradling et al., 1999, Perrimon et al., 1996)

93E4-93E5

94E3--7

(BDGP Project Members, 1994-1999)

94E3-94E4

(determined by in situ hybridisation)

(Mohler et al., 1991)

Experimentally Determined Recombination Data

Location

3-78

(FlyBase, 2016)

3-79.9

(Comeron et al., 2012)

3-81.2

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (23)

29092

w¹¹¹⁸; Mi{ET1}cnc^MB10127

25984

y¹ v¹; P{TRiP.JF02006}attP2

40854

y¹ v¹; P{TRiP.HMS02021}attP40

77686

w¹¹¹⁸; PBac{IT.GAL4}cnc^5001-G4

17502

y¹ w^67c23; P{EPgy2}cnc^EY08884

F004795

M{UAS-cnc.ORF-VN}ZH-86Fb

F000602

M{UAS-cnc.ORF.3xHA.GW}ZH-86Fb

124434

w¹¹¹⁸; P{RS3}cnc^UM-8235-3

v101235

P{KK103976}VIE-260B

v108127

P{KK101639}VIE-260B

v37673

w¹¹¹⁸; P{GD4437}v37673

v37674

w¹¹¹⁸; P{GD4437}v37674

More stocks available...

Genomic Clones (32)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (116)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_RE05559

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0262975

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

42743

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Deng and Kerppola, 2013, McGinnis et al., 1998)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: cnc anon-WO0153538.6 anon-WO0153538.7

(FlyBase, 1996-)

Source for merge of: cnc CG17894

(FlyBase, 1996-)

Source for merge of: CG13826 BcDNA:RE05559

(FlyBase, 1996-)

Source for merge of: cnc CG13826

(FlyBase Genome Annotators, 2011)

Additional comments

Annotations CG17894 and CG13826 merged as CG43286 in release 5.35 of the genome annotation.

(FlyBase Genome Annotators, 2011)

Annotations CG4566, CG4578 and CG17894 merged as CG17894 (which corresponds to cnc) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of cnc anon-WO0153538.6 anon-WO0153538.7 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Source for merge of cnc CG17894 was a shared cDNA ( date:010720 ).

(FlyBase, 1996-)

Source for merge of CG13826 BcDNA:RE05559 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Other Comments

DNA-protein interactions: genome-wide binding profile assayed for cnc protein in 0-12 hr embryos; see mE1_TFBS_cnc collection report.

(Negre et al., 2011)

Identification: in a germline clone screen for mutants that are defective in localisation of an Avic\GFP-stau marker in living oocytes. 3 alleles of cnc have been identified in the screen.

(Martin et al., 2003)

cnc has diverse functions in anterior-posterior patterning in the embryonic head.

(Veraksa et al., 2000)

Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.

(Harvie et al., 1998)

The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(w^hs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. cnc is required for germ cell viability or early oogenesis.

(Perrimon et al., 1996)

cnc interacts with Dfd, hh and shd. Null mutations delete both labral and mandibular structures.

(Gellon et al., 1995)

Mutations of cnc interact with Dfd to reduce the viability of the Dfd³/Dfd¹³ combination.

(Harding et al., 1995)

cnc functions in combination with Dfd to specify mandibular development and is also required for labral segment identity. fkh and cnc homeotic genes are required for the maintenance of segment polarity gene expression in the foregut. cnc is required for the continued expression of the dorsal pharyngeal domains of wg and hh expression past stage 10 and the labral domain of wg expression past the middle stage 11.

(Mohler et al., 1995)

In its anterior domain (labral primordia) cnc is activated by bcd and tor maternal pathways, but activation in intercalary and mandibular primordia requires zygotic gap gene products, activation by btd and repression by oc (anteriorly) or sna (ventrally).

(Mohler, 1993)

In situ hybridization reveals cnc transcripts are localised to the mandibular segment and the hypopharyngeal and labial primordia, first detectable in late blastoderm stages. Sequence analysis of cnc gene product reveals similarities to transcription factors of the leucine zipper (bZIP) class.

(Mohler et al., 1991)

Expression pattern and DNA sequence data suggest a role of cnc in cephalic patterning during embryogenesis.

(Mohler et al., 1991)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 117 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42743

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P20482

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0262975

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0262975

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

42743

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031486

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG43286

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P20482

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262975

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

cnc

MIST (protein-protein) - An integrated Molecular Interaction Database

cnc

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Factors involved in megakaryocyte development and platelet production

Synonyms and Secondary IDs (39)

Reported As

Symbol Synonym

5134

(Harvie et al., 1998)

BcDNA:RE05559

CG13826

(Cingolani et al., 2012, Nechaev et al., 2010, Keleman et al., 2009.8.5)

CG17894

(Reinke et al., 2013, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Mohit et al., 2006, Molnar et al., 2006, Laviolette et al., 2005)

CG43286

(Hosono et al., 2015, FlyBase Genome Annotators, 2011, Ni et al., 2010.12.1)

CG4566

(Wang et al., 2004, Luschnig, 2003.12.17)

CG4578

(Ruden, 2002.1.7)

CNC

(Abolaji et al., 2015, Mohler, 1993.6.22)

CNC_DROME

(Bornberg-Bauer et al., 1998)

CnC

(Wang et al., 2014, Gems and Partridge, 2013)

Cnc

(Fujisawa et al., 2020, Makhnovskii et al., 2020, Kwon et al., 2018, Fuse and Kobayashi, 2017, Bott et al., 2016, Jain et al., 2015, Lacher et al., 2015, Pitoniak and Bohmann, 2015, Zhan et al., 2015, Slattery et al., 2014, Webber et al., 2013, Rand et al., 2009, Veraksa et al., 2000)

Cnc-C

(Pomatto et al., 2017)

DM12

(Reinke et al., 2013)

NRF2

(Khezri and Rusten, 2019, Soares et al., 2018, Ferrandon, 2013)

anon-WO0153538.6

anon-WO0153538.7

cnc

(Bayliak et al., 2020, Cho et al., 2020, Chougule et al., 2020, Fernández-Cruz et al., 2020, Zhang et al., 2020, Bouska et al., 2019, Everman et al., 2019, Gumeni et al., 2019, Meltzer et al., 2019, Nash et al., 2019, Shokri et al., 2019, Trinh et al., 2019, Villanueva-Cañas et al., 2019, Wang et al., 2019, Bischof et al., 2018, Nath et al., 2018, Stapper and Jahn, 2018, Karaiskos et al., 2017, Tokusumi et al., 2017, Transgenic RNAi Project members, 2017-, Castillo-Quan et al., 2016, Clandinin and Owens, 2016-, Fear et al., 2016, Loboda et al., 2016, Peng et al., 2016, Schueren and Thoms, 2016, Williams et al., 2016, Baëza et al., 2015, Barckmann et al., 2015, de Taffin et al., 2015, Matthews et al., 2015, Schertel et al., 2015, Ashwal-Fluss et al., 2014, Boyle et al., 2014, Combs and Eisen, 2013, Deng and Kerppola, 2013, Hwang et al., 2013, Iyer et al., 2013, Nagy et al., 2013, Rahman et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Mackay et al., 2012, Pezzulo et al., 2012, Spokony and White, 2012.5.22, Vamos and Boros, 2012, Bantignies et al., 2011, Barone et al., 2011, Grimberg et al., 2011, Hochmuth et al., 2011, Jungreis et al., 2011, Jungreis et al., 2011, Kaplan et al., 2011, Michaut et al., 2011, Nègre et al., 2011, Ntini and Wimmer, 2011, Mourikis et al., 2010, The modENCODE Consortium, 2010, The modENCODE Consortium, 2010, Christensen et al., 2009.5.6, Economou and Telford, 2009, Hazelett et al., 2009, Löhr et al., 2009, Platts et al., 2009, Zsindely et al., 2009, Christensen et al., 2008.9.29, Garcia-Lopez et al., 2008, Segal et al., 2008, Sykiotis and Bohmann, 2008, Geng and MacDonald, 2007, Kaplow et al., 2007, Shravage et al., 2007, Molnar et al., 2006, Laviolette et al., 2005, Gurunathan et al., 2004)

cnc-C

(Pickering et al., 2013)

cncC

(Saeedi et al., 2020, Bayliak et al., 2019, Evangelakou et al., 2019, Zhang et al., 2019, Ng et al., 2017, Tsakiri et al., 2017, Whon et al., 2017, Wang et al., 2015)

cncC/Nrf2

(Manola et al., 2019)

dNrf2

(Zhou et al., 2015)

l(3)03921

(Perrimon et al., 1996)

l(3)j5E7

(Spradling et al., 1999)

nrf2

(Saini et al., 2020, Boulan et al., 2019)

Name Synonyms

Cap 'n' collar

(Fuse and Kobayashi, 2017, Veraksa et al., 2000, Veraksa et al., 2000)

Cap'n'collar

(Barone et al., 2011, Ishihara and Shibata, 2008)

Cap'n'collar B

(Ntini and Wimmer, 2011)

Cap-and-collar

(Dialynas et al., 2015)

Cap-n-colar

(Zhan et al., 2015)

Cap-n-collar

(Ahmed-de-Prado and Baonza, 2018)

cap "n" collar

(Economou and Telford, 2009)

cap 'n' collar

(Ntini and Wimmer, 2011, Gellon et al., 1995)

cap and collar

(Nagy et al., 2013, Zsindely et al., 2009, Dubois and Vincent, 2001)

cap n collar

(Kaplow et al., 2007)

cap'n'collar

(Shravage et al., 2007, Seecoomar et al., 2000)

cap-n-collar

(Tokusumi et al., 2017, Löhr et al., 2009, Coiffier et al., 2008, Garcia-Lopez et al., 2008)

nuclear factor E2-related factor 2

(Zhu et al., 2017)

Secondary FlyBase IDs

FBgn0000338
FBgn0010824
FBgn0039062
FBgn0039063
FBgn0045881
FBgn0045892
FBgn0040590
FBgn0047182

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

modENCODE_regulation_TFs

genome binding

Genome-wide localization of transcription factors by ChIP-chip and ChIP-Seq.

References (281)