Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
There is only one protein coding transcript and one polypeptide associated with this gene
1461 (aa); 165 (kD observed); 165 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\BubR1 using the Feature Mapper tool.
Bub1 protein is detected at the kinetochore in chromosomes from colchicine treated S2 cells and larval neuroblasts. Bub1 protein shows cell-cycle dependent localization. Diffuse nucleoplasmic staining is detected at interphase. Strong kinetochore localization is found in prophase. This staining is weaker at prometaphase. At metaphase, no Bub1 protein is detected at chromosomes at the metaphase plate. Weak kintetochore staining may be seen in early anaphase, but not in late anaphase or telophase. Localization of Bub1 protein shows a similar dynamic distribution pattern in the first and second meiotic divisions during spermatogenesis.
GBrowse - Visual display of RNA-Seq signalsView Dmel\BubR1 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: BubR1 CG7838
Source for merge of: BubR1 mcl(2)Z1525
Previous molecular and genetic analysis (FBrf0109303) identified the CG7838 annotation as having significant homology to "Bub1" proteins from other organisms (FlyBase curator comment: this resulted in the "CG7838" gene being renamed to "Bub1" in FlyBase). However, the completion of the Drosophila genome revealed the existence of a second annotation, CG14030, that is also closely related to "Bub1" proteins. Higher eukaryotes also have two genes encoding proteins closely related to "Bub1", one of which retains the symbol "Bub1", while the other is called "BubR1". From phylogenetic analysis including both CG7838 and CG14030 it is difficult to determine unequivocally which gene codes for "Bub1" and which gene codes for "BubR1". However, functional analysis of these proteins in FBrf0174806 suggests that CG14030 encodes the homolog of Bub1 (FlyBase curator comment: as a consequence of this, the gene corresponding to the CG7838 annotation has been renamed "BubR1" and the gene corresponding to the CG14030 annotation has been renamed "Bub1" in FlyBase).
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
dsRNA against this gene has been used to treat SL2 cells to study the effect of depletion of BubR1 on the spindle checkpoint.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, aneuploidy, an increase in mitotic index, a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells, a whole range of mitotic abnormalities, central spindle defects, chromosome abnormalities, and lagging chromatids are seen.
Loss of function mutations in BubR1 cause severe mitotic abnormalities consistent with accelerated transit through metaphase.
Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.