FB2026_02 , released June 18, 2026
Gene: Dmel\BubR1
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General Information
Symbol
Dmel\BubR1
Species
D. melanogaster
Name
Bub1-related kinase
Annotation Symbol
CG7838
Feature Type
protein_coding_gene
FlyBase ID
FBgn0263855
Gene Model Status
Current
Stock Availability
23 publicly available
Gene Summary
Bub1-related kinase (BubR1) encodes a motif-rich protein with a C-terminal serine-threonine kinase domain. During prometaphase and metaphase, it plays important roles in spindle assembly checkpoint signalling, stabilization of kinetochore-microtuble attachments and chromosome alignment. It is also essential to maintain sister-chromatid cohesion during meiotic progression and for normal maintenance of Synaptonemal Complex in females. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

bub1, l(2)k03113

Key Links
Genomic Location
Cytogenetic map
42A5-42A6
Sequence location
Recombination map
2-55
RefSeq locus
NT_033778 REGION:5969099..5974385
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (14 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441
(InterPro Project Members, 2004-)
enables protein kinase activity
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000361607
(GO Reference Genome Project, 2011-)
Biological Process (9 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
involved_in cellular response to starvation
inferred from mutant phenotype
(Liu et al., 2024)
involved_in female meiosis sister chromatid cohesion
inferred from mutant phenotype
(Malmanche et al., 2007)
involved_in heterochromatin formation
inferred from mutant phenotype
(Torras-Llort et al., 2010)
involved_in male meiosis sister chromatid cohesion
inferred from mutant phenotype
(Malmanche et al., 2007)
involved_in meiotic sister chromatid cohesion, centromeric
inferred from mutant phenotype
(Malmanche et al., 2007)
involved_in mitotic spindle assembly checkpoint signaling
inferred from mutant phenotype
(Rahmani et al., 2009, Perez-Mongiovi et al., 2005, Basu et al., 1999)
inferred from direct assay
(Logarinho et al., 2004)
involved_in regulation of exit from mitosis
inferred from mutant phenotype
(Garner et al., 2001)
inferred from genetic interaction with FLYBASE:dup; FB:FBgn0000996
(Garner et al., 2001)
involved_in regulation of mitotic sister chromatid separation
inferred from mutant phenotype
(Rahmani et al., 2009)
involved_in synaptonemal complex organization
inferred from mutant phenotype
(Malmanche et al., 2007)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in meiotic sister chromatid cohesion, centromeric
inferred from biological aspect of ancestor with PANTHER:PTN000361607
(GO Reference Genome Project, 2011-)
involved_in mitotic spindle assembly checkpoint signaling
inferred from electronic annotation with InterPro:IPR015661
(InterPro Project Members, 2004-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Buffin et al., 2005)
located_in kinetochore
inferred from direct assay
(Delcros et al., 2006, Buffin et al., 2005, Dzhindzhev et al., 2005, Logarinho et al., 2004)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000361608
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Bub1-related kinase (BubR1) encodes a motif-rich protein with a C-terminal serine-threonine kinase domain. During prometaphase and metaphase, it plays important roles in spindle assembly checkpoint signalling, stabilization of kinetochore-microtuble attachments and chromosome alignment. It is also essential to maintain sister-chromatid cohesion during meiotic progression and for normal maintenance of Synaptonemal Complex in females. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
UNCLASSIFIED CONVENTIONAL PROTEIN KINASE DOMAIN KINASES -
This group comprises conventional protein kinase domain kinases that do not classify under other groups in FlyBase.
Summary (Interactive Fly)

protein serine/threonine kinase - mitotic checkpoint control protein - inhibits ubiquitin ligase activity of anaphase promoting complex (APC) preventing mitosis until all chromosomes are correctly attached to the mitotic spindle

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\BubR1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry A1Z6I7)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
BubR1-RA
FBtr0086032
NM_058034
4626
1460
Additional Transcript Data and Comments
Reported size (kB)

4.601 (longest cDNA)

(Basu et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
BubR1-PA
FBpp0085368
165.1
1460
6.27
A1Z6I7
NP_477382
AAF57298
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

1461 (aa); 165 (kD observed); 165 (kD predicted)

(Basu et al., 1999)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\BubR1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.12

Transcript Expression
Polypeptide Expression
Additional Descriptive Data

Bub1 protein is detected at the kinetochore in chromosomes from colchicine treated S2 cells and larval neuroblasts. Bub1 protein shows cell-cycle dependent localization. Diffuse nucleoplasmic staining is detected at interphase. Strong kinetochore localization is found in prophase. This staining is weaker at prometaphase. At metaphase, no Bub1 protein is detected at chromosomes at the metaphase plate. Weak kintetochore staining may be seen in early anaphase, but not in late anaphase or telophase. Localization of Bub1 protein shows a similar dynamic distribution pattern in the first and second meiotic divisions during spermatogenesis.

(Basu et al., 1999)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Buffin et al., 2005)
located_in kinetochore
inferred from direct assay
(Delcros et al., 2006, Buffin et al., 2005, Dzhindzhev et al., 2005, Logarinho et al., 2004)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\BubR1 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 12 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 43 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of BubR1
Transgenic constructs containing regulatory region of BubR1
Aberrations (Deficiencies and Duplications) ( 30 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4insc-Mz1407
lethal, with Scer\GAL4αTub84B.PL
lethal | recessive
partially lethal - majority live
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
viable (with BubR1D1326N)
viable (with BubR1k06109)
viable (with BubR1Rev1)
viable (with Df(2R)nap9)
Sterility
Allele
female sterile
female sterile, with Scer\GAL4VP16.nanos.UTR, Scer\GAL4VP16.otu, Scer\GAL4nanos.PG
fertile
male fertile
Other Phenotypes
Allele
abnormal cell cycle
abnormal meiotic cell cycle (with BubR1D1326N)
abnormal meiotic cell cycle (with BubR1k06109)
abnormal meiotic cell cycle (with BubR1Rev1)
abnormal meiotic cell cycle (with Df(2R)nap9)
abnormal meiotic cell cycle | recessive
abnormal mitotic cell cycle
abnormal mitotic cell cycle (with BubR1k06109)
abnormal mitotic cell cycle (with BubR1Rev1)
abnormal mitotic cell cycle | embryonic stage 2
abnormal mitotic cell cycle | embryonic stage 3
abnormal mitotic cell cycle | embryonic stage 4
abnormal mitotic cell cycle | recessive
abnormal mitotic cell cycle | third instar larval stage, with BubR1k06109
abnormal mitotic cell cycle | third instar larval stage, with BubR1KEN.mRFP1
abnormal neuroanatomy | larval stage, with Scer\GAL4insc-Mz1407
decreased cell number | adult stage
decreased cell number | oogenesis
increased cell death | larval stage
increased cell death | third instar larval stage, with Scer\GAL4en-e16E
increased mortality | nutrition conditional, with Scer\GAL4Act5C.PU
increased mortality | nutrition conditional, with Scer\GAL4Cg.PA
increased mortality | nutrition conditional, with Scer\GAL4r4
increased mortality | nutrition conditional (with BubR1k03113)
increased mortality | nutrition conditional (with BubR1MI01546)
long lived
visible, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
adult enteroendocrine cell | decreased number
adult fat body | nutrition conditional
adult fat body | nutrition conditional, with Scer\GAL4Act5C.PU
adult fat body | nutrition conditional, with Scer\GAL4Cg.PA
adult fat body | nutrition conditional, with Scer\GAL4r4
chromosome
condensed chromosome
condensed chromosome (with BubR1k06109)
condensed chromosome (with BubR1Rev1)
embryo
fat body | nutrition conditional (with BubR1k03113)
fat body | nutrition conditional (with BubR1MI01546)
ganglion mother cell | larval stage, with Scer\GAL4insc-Mz1407
germarium cap cell | decreased number
imaginal disc
intestinal stem cell | adult stage | decreased number
larval brain | larval stage
larval neuroblast | larval stage, with Scer\GAL4insc-Mz1407
lipid droplet | nutrition conditional, with Scer\GAL4Act5C.PU
lipid droplet | nutrition conditional, with Scer\GAL4Cg.PA
lipid droplet | nutrition conditional, with Scer\GAL4r4
lipid droplet | nutrition conditional (with BubR1k03113)
lipid droplet | nutrition conditional (with BubR1MI01546)
meiotic cell cycle | male
mitosis & nuclear chromosome
mitotic anaphase
mitotic anaphase & nuclear chromosome
mitotic cell cycle
neuroblast
neuroblast (with BubR1k06109)
neuroblast (with BubR1Rev1)
nucleus
onion stage spermatid
onion stage spermatid & nucleus
spindle
spindle microtubule
trichogen cell, with Scer\GAL4pnr-MD237
wing disc posterior compartment | third instar larval stage, with Scer\GAL4en-e16E
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (9)
Hsap\BUB1
12 of 14
Yes
Yes
Hsap\BUB1B
6 of 14
No
Yes
1  
Hsap\ARAF
1 of 14
No
No
Hsap\CIT
1 of 14
No
No
1  
Hsap\LCK
1 of 14
No
No
Hsap\MAP3K11
1 of 14
No
No
Hsap\PBK
1 of 14
No
No
Hsap\TP53RK
1 of 14
No
No
1  
Hsap\UHMK1
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (8)
Rnor\Bub1
12 of 14
Yes
Yes
Rnor\Bub1b
6 of 14
No
Yes
Rnor\Araf
1 of 14
No
No
Rnor\Lck
1 of 14
No
No
Rnor\LOC685619
1 of 14
No
No
Rnor\Map3k11
1 of 14
No
No
Rnor\Tp53rk
1 of 14
No
No
Rnor\Uhmk1
1 of 14
No
Yes
Mus musculus (laboratory mouse) (9)
Mmus\Bub1
12 of 14
Yes
Yes
Mmus\Bub1b
5 of 14
No
Yes
Mmus\Araf
1 of 14
No
No
Mmus\Eif2ak2
1 of 14
No
No
Mmus\Lck
1 of 14
No
No
Mmus\Map3k11
1 of 14
No
No
Mmus\Trp53rka
1 of 14
No
No
Mmus\Trp53rkb
1 of 14
No
No
Mmus\Uhmk1
1 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (5)
Xtro\bub1
9 of 13
Yes
Yes
Xtro\bub1b
4 of 13
No
Yes
Xtro\lck
1 of 13
No
No
Xtro\slc22a2
1 of 13
No
No
Xtro\uqcrfs1
1 of 13
No
No
Danio rerio (Zebrafish) (12)
Drer\bub1
11 of 14
Yes
Yes
Drer\bub1bb
5 of 14
No
Yes
Drer\bub1ba
2 of 14
No
Yes
Drer\araf
1 of 14
No
No
Drer\blk
1 of 14
No
No
Drer\ern2
1 of 14
No
No
Drer\LOC799644
1 of 14
No
Yes
Drer\LOC100150106
1 of 14
No
Yes
Drer\si:ch211-147h1.4
1 of 14
No
Yes
Drer\si:ch211-160d20.5
1 of 14
No
Yes
Drer\si:dkey-248f6.3
1 of 14
No
Yes
Drer\tp53rk
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\bub-1
8 of 14
Yes
Yes
Cele\san-1
3 of 14
No
Yes
Cele\air-1
1 of 14
No
No
Cele\F52C12.6
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (5)
Agam\AgaP_AGAP011124
5 of 12
Yes
Yes
Agam\AgaP_AGAP003332
3 of 12
No
No
Agam\AgaP_AGAP000234
1 of 12
No
No
Agam\AgaP_AGAP004594
1 of 12
No
No
Agam\AgaP_AGAP007476
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (10)
Atha\AT2G20635
10 of 13
Yes
Yes
Atha\BUBR1
6 of 13
No
Yes
Atha\AT5G05510
4 of 13
No
Yes
Atha\AT3G03940
1 of 13
No
No
Atha\AT3G15890
1 of 13
No
No
Atha\AT3G46930
1 of 13
No
No
Atha\AT5G26110
1 of 13
No
No
Atha\AT5G58950
1 of 13
No
No
Atha\AT5G66710
1 of 13
No
No
Atha\HT1
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (8)
Scer\BUB1
10 of 13
Yes
Yes
Scer\MAD3
5 of 13
No
Yes
Scer\BUD32
1 of 13
No
No
Scer\KNS1
1 of 13
No
No
Scer\MEK1
1 of 13
No
No
Scer\PKH3
1 of 13
No
No
Scer\RCK2
1 of 13
No
No
Scer\SSK2
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (3)
Spom\bub1
9 of 12
Yes
Yes
Spom\mad3
3 of 12
No
Yes
Spom\SPAP27G11.07c
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:BubR1. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (7)
Bub1
10 of 13
CaMKI
1 of 13
CG12147
1 of 13
Ire1
1 of 13
S6KL
1 of 13
slpr
1 of 13
Tcs5
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 4 )
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
BubR1DN.UAS
ameliorates  autosomal dominant cerebellar ataxia
modeled by Hsap\ATXN182Q.UAS
(Park et al., 2013)
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
BUB1; BUB1 mitotic checkpoint serine/threonine kinase
12 of 14
602452
BUB1B; BUB1 mitotic checkpoint serine/threonine kinase B
6 of 14
602860
 
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
Interaction Browsers
View in FlyBase Interactions Browser   View in MIST tool (external link)
Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
bsk|BubR1
suppressible
dap
(Wong et al., 2014)
BubR1
suppressible
InR
(Tang et al., 2020)
BubR1
enhanceable
Pepck1
(Hussain et al., 2017)
BubR1
suppressible
Rel
(Liu et al., 2024)
BubR1
enhanceable
Sas-4
(Caous et al., 2015)
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
asp
suppressible
BubR1
(Rahmani et al., 2009, Basto et al., 2000)
cid
suppressible
BubR1
(Blower et al., 2006)
dup
suppressible
BubR1
(Garner et al., 2001)
polo
suppressible
BubR1
(Donaldson et al., 2001)
Sas-4
enhanceable
BubR1
(Caous et al., 2015)
Sas-4
suppressible
BubR1
(Caous et al., 2015)
sub
suppressible
BubR1
(Cesario et al., 2006)
External Data
Linkouts
DroID - A comprehensive database of gene and protein interactions.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
FlyBase
External Links
External Data
Linkouts
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
SignaLink - A signaling pathway resource with multi-layered regulatory networks.
Class of Gene
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-55
Cytogenetic map
42A5-42A6
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
42A5-42A6
Limits computationally determined from genome sequence between P{PZ}l(2)0985109851&P{lacW}Src42Ak10108 and P{lacW}l(2)k09848k09848&P{EP}EP407
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
42A1-42A2
(determined by in situ hybridisation)
(Spradling et al., 1999)
42A1-42A3
(determined by in situ hybridisation)
(Basu et al., 1999)
42A1-42A2
(BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location

2-55

(FlyBase, 2016)

2-55.7

(Comeron et al., 2012)

2-

(Wakimoto et al., 2004)
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (23)
Genomic Clones (13)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (43)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi and Array Information
Linkouts
Antibody Information
Laboratory Generated Antibodies
 

polyclonal

(Maia et al., 2007, Logarinho et al., 2004, Basu et al., 1999)
Commercially Available Antibodies
 
Cell Line Information
Publicly Available Cell Lines
 
    Other Stable Cell Lines
     
      Other Comments

      RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

      (Goshima et al., 2007)

      dsRNA against this gene has been used to treat SL2 cells to study the effect of depletion of BubR1 on the spindle checkpoint.

      (Muller et al., 2006)

      When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, aneuploidy, an increase in mitotic index, a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells, a whole range of mitotic abnormalities, central spindle defects, chromosome abnormalities, and lagging chromatids are seen.

      (Bettencourt-Dias et al., 2004)

      BubR1 has a role in blocking exit from mitosis.

      (Garner et al., 2001)

      Loss of function mutations in BubR1 cause severe mitotic abnormalities consistent with accelerated transit through metaphase.

      (Basu et al., 1999)

      Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.

      (Roch et al., 1998)
      Relationship to Other Genes
      Source for database merge of

      Source for merge of: BubR1 mcl(2)Z1525

      (Malmanche et al., 2007)
      Additional comments
      Nomenclature History
      Source for database identify of

      Source for identity of: BubR1 CG7838

      (Logarinho et al., 2004)
      Nomenclature comments

      Previous molecular and genetic analysis (FBrf0109303) identified the CG7838 annotation as having significant homology to "Bub1" proteins from other organisms (FlyBase curator comment: this resulted in the "CG7838" gene being renamed to "Bub1" in FlyBase). However, the completion of the Drosophila genome revealed the existence of a second annotation, CG14030, that is also closely related to "Bub1" proteins. Higher eukaryotes also have two genes encoding proteins closely related to "Bub1", one of which retains the symbol "Bub1", while the other is called "BubR1". From phylogenetic analysis including both CG7838 and CG14030 it is difficult to determine unequivocally which gene codes for "Bub1" and which gene codes for "BubR1". However, functional analysis of these proteins in FBrf0174806 suggests that CG14030 encodes the homolog of Bub1 (FlyBase curator comment: as a consequence of this, the gene corresponding to the CG7838 annotation has been renamed "BubR1" and the gene corresponding to the CG14030 annotation has been renamed "Bub1" in FlyBase).

      (Logarinho et al., 2004)
      Etymology
      Synonyms and Secondary IDs (20)
      Reported As
      Symbol Synonym
      31/13
      (Roch et al., 1998)
      BUB1
      (Williams et al., 2003)
      BUBR1
      (Heun et al., 2006)
      BcDNA:LD23835
      Bub1
      (De Lucia et al., 2005)
      BubR1
      (Shapiro et al., 2025, Joshi et al., 2024, Vesala et al., 2024, Shinoda et al., 2023, Warecki et al., 2023, Beaver et al., 2022, Khan et al., 2021, Ostalé et al., 2021, Cunha-Silva et al., 2020, Tang et al., 2020, Huang et al., 2019, Carvalhal et al., 2018, Levis, 2018.8.30, Resende et al., 2018, Tsui et al., 2018, Das et al., 2016, Derive et al., 2015, Liang et al., 2014, Rowshanravan et al., 2014, Rubin et al., 2014, Sopko et al., 2014, Wong et al., 2014, Cane et al., 2013, Cipressa and Cenci, 2013, Conde et al., 2013, Feijão et al., 2013, Morais da Silva et al., 2013, Park et al., 2013, Schertel et al., 2013, Althoff et al., 2012, Delgehyr et al., 2012, Di Stefano et al., 2011, Neumüller et al., 2011, Przewloka et al., 2011, Shieh and Bonini, 2011, Toku et al., 2011, Venkei et al., 2011, Li et al., 2010, Nishiyama et al., 2010, Oliveira et al., 2010, Podhraski et al., 2010, Royou et al., 2010, Schittenhelm et al., 2010, Torras-Llort et al., 2010, Bucciarelli et al., 2009, Graham et al., 2009, Lince-Faria et al., 2009, Martins et al., 2009, Matos et al., 2009, Rahmani et al., 2009, Schittenhelm et al., 2009, Kwon et al., 2008, Pauli et al., 2008, Wen et al., 2008, Buffin et al., 2007, Garcia et al., 2007, Gilliland et al., 2007, Lehner et al., 2007, Li et al., 2007, Maia et al., 2007, Orr et al., 2007, Pandey et al., 2007, Blower et al., 2006, Cesario et al., 2006, Delcros et al., 2006, Laycock et al., 2006, Muller et al., 2006, Buffin, 2005, Buffin et al., 2005, Buffin et al., 2005, Dzhindzhev et al., 2005, Goshima and Vale, 2005, Heeger et al., 2005, Karess, 2005, Maia and Sunkel, 2005, Malmanche and Sunkel, 2005, Page et al., 2005, Perez-Mongiovi et al., 2005, Royou et al., 2005, Royou et al., 2005, Abad et al., 2004, Bettencourt-Dias et al., 2004, Fischer et al., 2004, Fisk and Winey, 2004, Logarinho et al., 2004, Parks, 2004.5.12, Parry et al., 2003, Maiato et al., 2002)
      CG 7838
      (Buffin, 2005)
      CG7838
      (National Institute of Genetics Fly Stocks, 2022-, Meadows, 2017.1.11, Hosono et al., 2015, Lin et al., 2015, Moutinho-Pereira et al., 2013, Schertel et al., 2013, Ni et al., 2011.7.19, Goshima et al., 2007, Buffin et al., 2005, Logarinho et al., 2004, Jin et al., 2001, Morrison et al., 2000)
      DmBUB1
      (Tyler-Smith and Floridia, 2000)
      DmBubRI
      (Oliveira et al., 2010)
      Dmbub1
      (Lopes et al., 2000)
      bub1
      (Myster et al., 2004, Deak et al., 2003, Lee and Orr-Weaver, 2003, Champion et al., 2002, Donaldson et al., 2001, Basto et al., 2000, Basu et al., 1999, Basu et al., 1998)
      bubR1
      (Joy et al., 2024, Baonza et al., 2022, Vicars et al., 2021, Landmann et al., 2020, Wang et al., 2019, Silva et al., 2018, Duranteau et al., 2016, Caous et al., 2015, Gogendeau et al., 2015, Royou et al., 2010, Torras-Llort et al., 2010, Rahmani et al., 2009, Wainman et al., 2009, Castellanos et al., 2008, Cenci et al., 2008, Musarò et al., 2008, Malmanche et al., 2007, Williams et al., 2007, Cullen et al., 2005, Purdy et al., 2005)
      bubr1
      (Blower et al., 2006)
      dBUB1
      (Dobie et al., 1999)
      l(2)k03113
      (BDGP Project Members, 1994-1999)
      l(2)k06109
      (Beaton, 2000.1.31)
      mcl(2)Z1525
      Name Synonyms
      Bub1-related kinase
      bub related one
      (Castellanos et al., 2008)
      Secondary FlyBase IDs
      • FBgn0025458
      • FBgn0022226
      • FBgn0069180
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      Study result (0)
      Result
      Result Type
      Title
      External Crossreferences and Linkouts ( 50 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL - MARRVEL (model organism gene)
      PDB - An information portal to biological macromolecular structures
      Linkouts
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      DroID - A comprehensive database of gene and protein interactions.
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
      References (214)