Expression increases after the prepupal (+10 hr APF) ecdysone pulse.

Eip93F transcript is expressed in a subset of olfactory sense organs in the antenna. These include some sensilla labelled by MmusCd8a^{Or47b.330.T:AvicGFP} or MmusCd8a^{Or92a.AvicGFP.T:HsapMYC}.

Eip93F transcripts are first detected during metamorphosis on northern blots. Transcript levels increase following the late larval and prepupal ecdysteroid pulses, as well as immediately before adult eclosion. In dissected salivary glands, they are expressed only in prepupal glands and were shown to be induced by 20E. In situ hybridization and northern blot analysis of RNA isolated from dissected tissues shows that Eip93F transcripts are present in gut and at a lower level in fat body of early prepupae and in the CNS, gut, fat body, salivary glands and imaginal discs in late prepupae.

RNA blots were carried out on RNA extracted from staged larval and prepupal salivary glands. Eip93F transcripts are induced in apparent response to the prepupal ecdysone pulse paralleling the puffing response at 93F. Ectopic ftz-f1 expression leads to Eip93F induction in late-larval salivary glands.

Eip93F is expressed broadly in developing adult tissues during pupation, but is not expressed in larvae. In pupal wings, Eip93F expression is reduced in the wing veins in comparision to the rest of the developing wing blade. At 39h APF, Eip93F is expressed in stripes on the labellum that likely correspond to developing pseudotracheae.

On the pupal leg, Eip93F is present at 27h APF in bract, bristle precursor cells (external sensory organ precursor cells), and tormogen (socket) cells. By 30h APF, expression is limited to only bract and tormogen cells, and is further limited to only bract cells by 42h APF. In adults, bracts are found on the legs and the wings. However, in the abdomen at 42h APF, Eip93F is highly expressed in tormogen cells and "bract-equivalent cells" associated with bristle precursor (external sensory organ precursor) cells.