myosin, MRP, muscle myosin heavy chain, ifm(2)RU2, Sht
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Gene model reviewed during 5.56
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Terminal exon usage of Mhc shifts mid-development during muscle filament assembly (FBrf0221075).
Gene model reviewed during 6.02
4.5, 4.2 (unknown)
8.6, 8.0, 7.2 (northern blot)
155 (kD observed)
1962 (aa)
Mhc protein in used as a marker for the visceral muscles or their precursors, which overlie the epithelial foregut and hindgut.
3'' end
3' end
5'' end
corresponds to exon16 encoded by g1335628
one of several products generated by alternative splicing
this fragment corresponds to part of exon 17 encoded by g133629 and all of exon 18 encoded by g1335630; MHC2 RNA found in pupal adult stages only
this fragment corresponds to part of exon 17 encoded by g133629 and all of exon 19 encoded by g1335631; MHC1 RNA found in pupal-adult and embryo-larval stages
one of a couple of products generated by alternative splicing
At least 480 Mhc isoforms are theoretically
encoded by the gene when all the different possible combinations of
alternate exons are considered. The alternative exons encode sequences
close to or within regions thought to have important structural or
enzymatic functions. Putative functional domains and regions of
exceptional sequence conservation, however, are most often distributed in
both alternative and common exons. Some of the characterized domains map
to exons as follows: a conserved hydrophobic pocket sequence in exons 3-4,
the highly conserved ATP-binding domain in exon 4, the highly divergent
proteolytic cleavage site that defines the 25-50kD junction in exon 4, two
highly conserved regions of unidentified function in exons 4-6 and 8-9,
the 50-20kD junction in exon 10, the primary actin-binding site in exon
10, a secondary actin-binding site in exon 11, the sites of myosin light
chain binding in exon 12, the head-tail junction in exon 12, the hinge
region in exons 14-16, and the non-coiled tailpiece in exons 17-19.
Predicted from sequence data (J02788). Encoded by transcript containing exon 3a.
Predicted from sequence data (J02788). Encoded by transcript containing exon 3b.
Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
Alternative splicing exons contribute to the specialized contractile activities of different muscle types. Exon 3 encodes the hydrophobic pocket adjacent to the ATP-binding site, exon 9 is adjacent to the actin-binding domain, exon 11 is involved in actin-binding, exon 15 in the S2 hinge and exons 18 and 19 the non-coiled tail region.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mhc using the Feature Mapper tool.
Expression dependent on Mef2 levels.
Mhc is enriched in border follicle cells relative to follicle cells.
The Mhc transcript of this structure is the main form expressed in the indirect flight muscle.
The Mhc transcript of this structure is the main form expressed in the TDT (mesothoracic extracoxal depressor muscle 66) and in direct flight muscle 51.
The Mhc transcript of this structure is the main form expressed in direct flight muscle 52 and in esophageal muscle.
Comment: mesodermal cells
Comment: 0-2 hr AEL
Mhc protein in used as a marker for the visceral muscles or their precursors, which overlie the epithelial foregut and hindgut.
Mhc protein is expressed in the mesodermal cells of the developing embryonic foregut.
Mef2 protein is localized to the nuclei of all embryonic somatic and visceral muscle cells and muscle cell precursors.
The 155kD form of Mhc protein is mainly detected in testis.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Mhc in GBrowse 2
2-53
2-51.2
2-53.8
2-51.6
2-52.6
2-52.0
2-53.5
2-51.5
2-52.4
2-54.7
2-51.3
2-52 or 2-54.7
Map position of 26.1, 51.7 and 53.1 based on linkage mapping of Ifm(2)RU12, Ifm(2)RU13 and Ifm(2)RU14, respectively.
Map position of 53.8, 51.6, 52.6, 52.0, 53.5 and 51.5 based on linkage mapping of ifm(2)RU210, ifm(2)RU211, ifm(2)RU212, ifm(2)RU213, ifm(2)RU214, and ifm(2)RU215, respectively.
Maps close to Mhc.
Maps 2.8 +/- 0.4 units to the left of cn.
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
