FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\RhoGEF3
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General Information
Symbol
Dmel\RhoGEF3
Species
D. melanogaster
Name
Rho guanine nucleotide exchange factor 3
Annotation Symbol
CG43976
Feature Type
protein_coding_gene
FlyBase ID
FBgn0264707
Gene Model Status
Current
Stock Availability
23 publicly available
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

RhoGEF

Key Links
Genomic Location
Cytogenetic map
61B3-61C1
Sequence location
Recombination map
3-1
RefSeq locus
NT_037436 REGION:277437..305292
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (7 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables guanyl-nucleotide exchange factor activity
inferred from biological aspect of ancestor with PANTHER:PTN002911494
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with FLYBASE:GEFmeso; FB:FBgn0050115
(Gene Ontology Curators, 2002-)
inferred from electronic annotation with InterPro:IPR000219
(InterPro Project Members, 2004-)
Biological Process (5 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in actin cytoskeleton organization
inferred from mutant phenotype
(Nakamura et al., 2017)
involved_in melanotic encapsulation of foreign target
inferred from mutant phenotype
(Howell et al., 2012)
involved_in response to wounding
inferred from mutant phenotype
(Nakamura et al., 2017)
involved_in wound healing
inferred from mutant phenotype
(Nakamura et al., 2017)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in regulation of Rho protein signal transduction
inferred from sequence or structural similarity with FLYBASE:GEFmeso; FB:FBgn0050115
(Gene Ontology Curators, 2002-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in cytosol
inferred from biological aspect of ancestor with PANTHER:PTN002911494
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
RHO GUANINE NUCLEOTIDE EXCHANGE FACTORS -
RhoGEFs are Guanine Nucleotide Exchange Factors (GEFs) for Rho family GTPases. They include Dbl-homology domain-containing proteins (the largest family of RhoGEFs) and CZH2 domain/Dock family GEFs. (Adapted from FBrf0190957).
Gene Model and Products
Number of Transcripts
12
Number of Unique Polypeptides
10

Please see the JBrowse view of Dmel\RhoGEF3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9W0S2)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 6.32

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
RhoGEF3-RA
FBtr0333952
NM_138183
4541
1011
RhoGEF3-RB
FBtr0333955
NM_167823
3086
932
RhoGEF3-RC
FBtr0333954
NM_167825
3153
925
RhoGEF3-RE
FBtr0333953
NM_167824
3168
930
RhoGEF3-RF
FBtr0333956
NM_001144390
3564
1011
RhoGEF3-RG
FBtr0333957
NM_001144391
3258
920
RhoGEF3-RH
FBtr0333958
NM_138182
3501
1026
RhoGEF3-RJ
FBtr0333959
NM_001169836
3082
820
RhoGEF3-RK
FBtr0333960
NM_001169837
3157
820
RhoGEF3-RL
FBtr0333961
NM_138180
11114
3519
RhoGEF3-RM
FBtr0333962
NM_001274273
12307
3518
RhoGEF3-RQ
FBtr0479920
NM_001382119
3428
971
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
RhoGEF3-PA
FBpp0306075
112.1
1011
8.65
Q9W0R9
NP_612027
AAF47374
RhoGEF3-PB
FBpp0306078
103.2
932
7.23
Q8IRJ4
NP_728515
AAN11434
RhoGEF3-PC
FBpp0306077
102.5
925
7.70
Q8IRJ2
NP_728517
AAN11436
RhoGEF3-PE
FBpp0306076
103.0
930
7.23
Q8IRJ3
NP_728516
AAN11435
RhoGEF3-PF
FBpp0306079
112.1
1011
8.65
Q9W0R9
NP_001137862
ACL83218
RhoGEF3-PG
FBpp0306080
102.0
920
7.23
B7Z0H3
NP_001137863
ACL83219
RhoGEF3-PH
FBpp0306081
112.7
1026
6.09
Q9W0S0
NP_612026
AAF47373
RhoGEF3-PJ
FBpp0306082
91.6
820
6.83
E1JHV3
NP_001163307
ACZ94579
RhoGEF3-PK
FBpp0306083
91.6
820
6.83
E1JHV3
NP_001163308
ACZ94580
RhoGEF3-PL
FBpp0306084
384.0
3519
9.46
Q9W0S2
NP_612024
AAF47371
RhoGEF3-PM
FBpp0306085
383.9
3518
9.46
M9PBG8
NP_001261202
AGB93897
RhoGEF3-PQ
FBpp0428252
107.7
971
7.85
AAF47372
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1011 aa isoforms: RhoGEF3-PA, RhoGEF3-PF
820 aa isoforms: RhoGEF3-PJ, RhoGEF3-PK
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RhoGEF3 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.57

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
central brain anlage in statu nascendi
(Fisher et al., 2012)
dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
hindgut anlage in statu nascendi
(Fisher et al., 2012)
organism | gap expression pattern
(Fisher et al., 2012)
organism | segmentally repeated
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
embryonic stage 5
organism | pair rule expression pattern
(Hicks et al., 2001)
embryonic stage 6
amnioproctodeal invagination

Comment: late stage 6

(Hicks et al., 2001)
cephalic furrow

Comment: late stage 6

(Hicks et al., 2001)
cephalic furrow | posterior to

Comment: early stage 6

(Hicks et al., 2001)
embryonic stage 7 -- 8
organism | faint | ubiquitous
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9
organism | segmentally repeated

Comment: stripes partially overlap en stripes

(Hicks et al., 2001)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
embryonic epidermis | segmentally repeated | vicinity of

Comment: sub-epidermal cells at dorsolateral edge of each segment

(Hicks et al., 2001)
central brain primordium
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 13
visceral trunk mesoderm
(Hicks et al., 2001)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
embryonic stage 16
muscle attachment site
(Hicks et al., 2001)
prepupal stage P3
leg | ubiquitous
(Greenberg and Hatini, 2011)
Additional Descriptive Data

Expression in stage P3 pupal leg discs is ubiquitous.

(Greenberg and Hatini, 2011)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\RhoGEF3 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 19 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 16 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of RhoGEF3
Transgenic constructs containing regulatory region of RhoGEF3
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(3L)Exel6084
(Ryder, 2004.4.26)
Inferred from location ( 4 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (57)
Hsap\ARHGEF9
8 of 14
Yes
Yes
1  
Hsap\ARHGEF4
7 of 14
No
Yes
Hsap\SPATA13
7 of 14
No
Yes
Hsap\PREX1
3 of 14
No
Yes
Hsap\PREX2
3 of 14
No
Yes
Hsap\ARHGEF37
2 of 14
No
Yes
Hsap\ARHGEF38
2 of 14
No
Yes
Hsap\DNMBP
2 of 14
No
Yes
Hsap\TIAM1
2 of 14
No
No
4  
Hsap\TIAM2
2 of 14
No
No
Hsap\AKAP13
1 of 14
No
No
Hsap\ARHGEF1
1 of 14
No
No
Hsap\ARHGEF2
1 of 14
No
No
Hsap\ARHGEF3
1 of 14
No
No
Hsap\ARHGEF5
1 of 14
No
No
Hsap\ARHGEF6
1 of 14
No
No
Hsap\ARHGEF7
1 of 14
No
No
1  
Hsap\ARHGEF12
1 of 14
No
No
Hsap\ARHGEF15
1 of 14
No
No
Hsap\ARHGEF16
1 of 14
No
No
Hsap\ARHGEF17
1 of 14
No
No
1  
Hsap\ARHGEF19
1 of 14
No
No
Hsap\ARHGEF25
1 of 14
No
No
Hsap\ARHGEF26
1 of 14
No
No
Hsap\ECT2
1 of 14
No
No
Hsap\ECT2L
1 of 14
No
No
Hsap\FARP1
1 of 14
No
No
Hsap\FARP2
1 of 14
No
No
Hsap\FGD1
1 of 14
No
No
Hsap\FGD2
1 of 14
No
No
Hsap\FGD3
1 of 14
No
No
Hsap\FGD4
1 of 14
No
No
Hsap\FGD5
1 of 14
No
No
Hsap\FGD6
1 of 14
No
No
Hsap\ITSN1
1 of 14
No
No
Hsap\ITSN2
1 of 14
No
No
1  
Hsap\KALRN
1 of 14
No
No
Hsap\MCF2
1 of 14
No
No
Hsap\MCF2L2
1 of 14
No
No
Hsap\MCF2L
1 of 14
No
No
Hsap\NET1
1 of 14
No
No
Hsap\NGEF
1 of 14
No
No
1  
Hsap\OBSCN
1 of 14
No
No
Hsap\PLEKHG1
1 of 14
No
No
Hsap\PLEKHG2
1 of 14
No
No
Hsap\PLEKHG3
1 of 14
No
No
Hsap\PLEKHG5
1 of 14
No
No
Hsap\PLEKHG6
1 of 14
No
No
1  
Hsap\PLEKHG7
1 of 14
No
No
Hsap\RASGRF1
1 of 14
No
No
Hsap\RASGRF2
1 of 14
No
No
Hsap\SOS1
1 of 14
No
No
Hsap\SOS2
1 of 14
No
No
1  
Hsap\TRIO
1 of 14
No
No
1  
Hsap\VAV1
1 of 14
No
No
1  
Hsap\VAV2
1 of 14
No
No
Hsap\VAV3
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (41)
Rnor\Arhgef4
8 of 14
Yes
Yes
Rnor\Spata13
8 of 14
Yes
Yes
Rnor\Arhgef9
7 of 14
No
Yes
Rnor\Arhgef37
2 of 14
No
Yes
Rnor\Dnmbp
2 of 14
No
Yes
Rnor\Prex1
2 of 14
No
Yes
Rnor\Prex2
2 of 14
No
Yes
Rnor\Tiam1
2 of 14
No
No
Rnor\Tiam2
2 of 14
No
No
Rnor\Akap13
1 of 14
No
No
Rnor\Arhgef1
1 of 14
No
No
Rnor\Arhgef2
1 of 14
No
No
Rnor\Arhgef6
1 of 14
No
No
Rnor\Arhgef7
1 of 14
No
No
Rnor\Arhgef12
1 of 14
No
No
Rnor\Arhgef17
1 of 14
No
No
Rnor\Arhgef19
1 of 14
No
No
Rnor\Arhgef25
1 of 14
No
No
Rnor\Arhgef26
1 of 14
No
No
Rnor\Arhgef28
1 of 14
No
No
Rnor\Arhgef38
1 of 14
No
Yes
Rnor\Ect2
1 of 14
No
No
Rnor\Farp2
1 of 14
No
No
Rnor\Fgd2
1 of 14
No
No
Rnor\Fgd3
1 of 14
No
No
Rnor\Itsn1
1 of 14
No
No
Rnor\Itsn2
1 of 14
No
No
Rnor\Mcf2l
1 of 14
No
No
Rnor\Mcf2
1 of 14
No
No
Rnor\Ngef
1 of 14
No
No
Rnor\Obscn
1 of 14
No
No
Rnor\Plekhg1
1 of 14
No
No
Rnor\Plekhg2
1 of 14
No
No
Rnor\Plekhg3
1 of 14
No
No
Rnor\Plekhg5
1 of 14
No
No
Rnor\Plekhg6
1 of 14
No
No
Rnor\Rasgrf2
1 of 14
No
Yes
Rnor\Trio
1 of 14
No
No
Rnor\Vav1
1 of 14
No
No
Rnor\Vav2
1 of 14
No
No
Rnor\Vav3
1 of 14
No
No
Mus musculus (laboratory mouse) (40)
Mmus\Arhgef4
8 of 14
Yes
Yes
Mmus\Arhgef9
8 of 14
Yes
Yes
Mmus\Spata13
8 of 14
Yes
Yes
Mmus\Prex1
3 of 14
No
Yes
Mmus\Prex2
3 of 14
No
Yes
Mmus\Arhgef37
2 of 14
No
Yes
Mmus\Arhgef38
2 of 14
No
Yes
Mmus\Tiam1
2 of 14
No
No
Mmus\Tiam2
2 of 14
No
No
Mmus\Arhgef2
1 of 14
No
No
Mmus\Arhgef6
1 of 14
No
No
Mmus\Arhgef7
1 of 14
No
No
Mmus\Arhgef10
1 of 14
No
No
Mmus\Arhgef12
1 of 14
No
No
Mmus\Arhgef16
1 of 14
No
No
Mmus\Arhgef17
1 of 14
No
No
Mmus\Arhgef19
1 of 14
No
No
Mmus\Arhgef25
1 of 14
No
No
Mmus\Arhgef26
1 of 14
No
No
Mmus\Arhgef28
1 of 14
No
No
Mmus\Dnmbp
1 of 14
No
Yes
Mmus\Ect2
1 of 14
No
No
2  
Mmus\Farp2
1 of 14
No
No
Mmus\Fgd3
1 of 14
No
No
Mmus\Itsn1
1 of 14
No
No
Mmus\Itsn2
1 of 14
No
No
Mmus\Kalrn
1 of 14
No
No
Mmus\Mcf2l
1 of 14
No
No
Mmus\Mcf2
1 of 14
No
No
Mmus\Ngef
1 of 14
No
No
Mmus\Obscn
1 of 14
No
No
Mmus\Plekhg1
1 of 14
No
No
Mmus\Plekhg2
1 of 14
No
No
Mmus\Plekhg3
1 of 14
No
No
Mmus\Plekhg5
1 of 14
No
No
Mmus\Plekhg6
1 of 14
No
No
Mmus\Trio
1 of 14
No
No
Mmus\Vav1
1 of 14
No
No
Mmus\Vav2
1 of 14
No
No
Mmus\Vav3
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (26)
Xtro\arhgef4
6 of 13
Yes
Yes
Xtro\spata13
5 of 13
No
Yes
Xtro\arhgef9
4 of 13
No
Yes
Xtro\prex2
2 of 13
No
Yes
Xtro\aass
1 of 13
No
No
Xtro\arhgef6
1 of 13
No
No
Xtro\arhgef7
1 of 13
No
No
Xtro\arhgef26
1 of 13
No
No
Xtro\arhgef38
1 of 13
No
Yes
Xtro\btk
1 of 13
No
No
Xtro\c1orf131
1 of 13
No
Yes
Xtro\farp1
1 of 13
No
No
Xtro\fgd1
1 of 13
No
No
Xtro\itsn2
1 of 13
No
No
Xtro\LOC100490228
1 of 13
No
No
Xtro\lrit2
1 of 13
No
No
Xtro\mcf2l
1 of 13
No
No
Xtro\net1
1 of 13
No
Yes
Xtro\ngef
1 of 13
No
No
Xtro\plekhg2
1 of 13
No
No
Xtro\plekhg5
1 of 13
No
No
Xtro\plekhg7
1 of 13
No
Yes
Xtro\prex1
1 of 13
No
Yes
Xtro\rasgrf2
1 of 13
No
Yes
Xtro\sos2
1 of 13
No
No
Xtro\trio
1 of 13
No
No
Danio rerio (Zebrafish) (48)
Drer\arhgef9a
8 of 14
Yes
Yes
Drer\arhgef9b
8 of 14
Yes
Yes
Drer\spata13
8 of 14
Yes
Yes
Drer\arhgef4
6 of 14
No
Yes
Drer\arhgef37
2 of 14
No
Yes
Drer\arhgef38
2 of 14
No
Yes
Drer\dnmbp
2 of 14
No
Yes
Drer\prex1
2 of 14
No
Yes
Drer\prex2
2 of 14
No
Yes
Drer\tiam1a
2 of 14
No
No
Drer\tiam1b
2 of 14
No
No
Drer\akap13
1 of 14
No
No
Drer\arhgef1b
1 of 14
No
No
Drer\arhgef3l
1 of 14
No
No
Drer\arhgef6
1 of 14
No
No
Drer\arhgef7a
1 of 14
No
No
Drer\arhgef7b
1 of 14
No
No
Drer\arhgef12a
1 of 14
No
No
Drer\arhgef12b
1 of 14
No
No
Drer\arhgef15a
1 of 14
No
No
Drer\arhgef15b
1 of 14
No
No
Drer\arhgef19
1 of 14
No
No
Drer\arhgef28a
1 of 14
No
No
Drer\ect2
1 of 14
No
No
Drer\farp2
1 of 14
No
No
Drer\fgd6
1 of 14
No
No
Drer\itsn1
1 of 14
No
No
Drer\itsn2a
1 of 14
No
No
Drer\itsn2b
1 of 14
No
No
Drer\mcf2a
1 of 14
No
No
Drer\mcf2l2
1 of 14
No
No
Drer\mcf2la
1 of 14
No
No
Drer\net1
1 of 14
No
No
Drer\plekhg1
1 of 14
No
No
Drer\plekhg2
1 of 14
No
No
Drer\plekhg5a
1 of 14
No
No
Drer\plekhg5b
1 of 14
No
No
Drer\plekhg6
1 of 14
No
No
Drer\plekhg7
1 of 14
No
No
Drer\si:ch73-15b2.5
1 of 14
No
No
Drer\si:dkey-91i10.2
1 of 14
No
No
Drer\si:dkey-172h23.2
1 of 14
No
No
Drer\tiam2a
1 of 14
No
No
Drer\trioa
1 of 14
No
No
Drer\triob
1 of 14
No
No
Drer\vav2
1 of 14
No
No
Drer\vav3b
1 of 14
No
No
Drer\vav3
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (11)
Cele\ephx-1
1 of 14
Yes
No
Cele\exc-5
1 of 14
Yes
No
Cele\osg-1
1 of 14
Yes
No
Cele\prhg-1
1 of 14
Yes
No
Cele\rhgf-1
1 of 14
Yes
No
Cele\rhgf-2
1 of 14
Yes
No
Cele\tag-52
1 of 14
Yes
No
Cele\tiam-1
1 of 14
Yes
No
Cele\uig-1
1 of 14
Yes
No
Cele\unc-73
1 of 14
Yes
No
Cele\vav-1
1 of 14
Yes
No
Anopheles gambiae (African malaria mosquito) (12)
Agam\AgaP_AGAP006382
8 of 12
Yes
Yes
Agam\AgaP_AGAP006107
1 of 12
No
No
Agam\AgaP_AGAP006514
1 of 12
No
No
Agam\AgaP_AGAP006590
1 of 12
No
No
Agam\AgaP_AGAP007452
1 of 12
No
No
Agam\AgaP_AGAP007685
1 of 12
No
No
Agam\AgaP_AGAP007723
1 of 12
No
No
Agam\AgaP_AGAP007877
1 of 12
No
No
Agam\AgaP_AGAP010292
1 of 12
No
No
Agam\AgaP_AGAP010472
1 of 12
No
No
Agam\AgaP_AGAP010473
1 of 12
No
No
Agam\AgaP_AGAP010767
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\CDC24
3 of 13
Yes
No
Scer\ROM1
1 of 13
No
No
Scer\ROM2
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (4)
Spom\scd1
2 of 12
Yes
No
Spom\gef1
1 of 12
No
No
Spom\gef3
1 of 12
No
No
Spom\rgf2
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:RhoGEF3. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (14)
sif
2 of 13
CG30440
1 of 13
CG42674
1 of 13
CG43102
1 of 13
cyst
1 of 13
Exn
1 of 13
GEFmeso
1 of 13
pbl
1 of 13
RhoGEF2
1 of 13
RhoGEF4
1 of 13
RhoGEF64C
1 of 13
RtGEF
1 of 13
trio
1 of 13
Vav
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ARHGEF9; Cdc42 guanine nucleotide exchange factor 9
    8 of 14
    300429
     
    ARHGEF4; Rho guanine nucleotide exchange factor 4
    7 of 14
    605216
        SPATA13; spermatogenesis associated 13
        7 of 14
        613324
            PREX1; phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1
            3 of 14
            606905
                PREX2; phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2
                3 of 14
                612139
                    Functional Complementation Data
                    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                    Interactions
                    Summary of Physical Interactions
                    Interaction Browsers
                    View in FlyBase Interactions Browser   View in MIST tool (external link)
                    Please see the Physical Interaction reports below for full details
                    protein-protein
                    Physical Interaction
                    Assay
                    References
                    RhoGEF3 - Hsap\CDC42
                    enzymatic study, fluorescence technology
                    (Couturier et al., 2017)
                    RhoGEF3 - Rac1
                    pull down, autoradiography
                    (Nakamura et al., 2017)
                    Summary of Genetic Interactions
                    Interaction Browsers
                    View in FlyBase Interactions Browser   View in MIST tool (external link)
                    Please look at the allele data for full details of the genetic interactions
                    Starting gene(s)
                    Interaction type
                    Interacting gene(s)
                    Reference
                    Starting gene(s)
                    Interaction type
                    Interacting gene(s)
                    Reference
                    Rac1
                    enhanceable
                    RhoGEF3
                    (Couturier et al., 2017)
                    External Data
                    Linkouts
                    DroID - A comprehensive database of gene and protein interactions.
                    Pathways
                    Signaling Pathways (FlyBase)
                    Metabolic Pathways
                    FlyBase
                    External Links
                    External Data
                    Linkouts
                    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                    Class of Gene
                    Genomic Location and Detailed Mapping Data
                    Chromosome (arm)
                    3L
                    Recombination map
                    3-1
                    Cytogenetic map
                    61B3-61C1
                    Sequence location
                    FlyBase Computed Cytological Location
                    Cytogenetic map
                    Evidence for location
                    61B3-61C1
                    Limits computationally determined from genome sequence between P{PZ}trh10512 and P{EP}EP3104EP3104
                    Experimentally Determined Cytological Location
                    Cytogenetic map
                    Notes
                    References
                    61B1-61B3
                    (Hicks, 2000.4.12)
                    Experimentally Determined Recombination Data
                    Location

                    3-1

                    (FlyBase, 2016)

                    3-0.1

                    (Comeron et al., 2012)
                    Left of (cM)
                    Right of (cM)
                    Notes
                    Stocks and Reagents
                    Stocks (23)
                    Genomic Clones (29)
                    cDNA Clones (110)
                     

                    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                    cDNA clones, fully sequenced
                    BDGP DGC clones
                    Other clones
                    Drosophila Genomics Resource Center cDNA clones

                    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                    cDNA Clones, End Sequenced (ESTs)
                    BDGP DGC clones
                    Other clones
                    RNAi and Array Information
                    Linkouts
                    Antibody Information
                    Laboratory Generated Antibodies
                     
                    Commercially Available Antibodies
                     
                    Cell Line Information
                    Publicly Available Cell Lines
                     
                      Other Stable Cell Lines
                       
                        Other Comments

                        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                        (Kiger et al., 2003)
                        Relationship to Other Genes
                        Source for database merge of

                        Source for merge of: RhoGEF3 CG42377

                        (FlyBase Genome Annotators, 2012)
                        Additional comments

                        Annotations CG42378 and CG42377 merged as CG43976 in release 5.46 of the genome annotation. Merge supported by RNA-seq junction data.

                        (FlyBase Genome Annotators, 2012)

                        Source for split of: CG13882

                        (FlyBase Genome Annotators, 2008)

                        Annotation CG13882 split in release 5.10 of the genome annotation. The 5' portion of CG13882 has been merged with the CG13882 annotation to create the annotation CG42377. The 3' portion of CG13882 has been merged with the CG13883 and CG1225 annotations to create annotation CG42378 (which corresponds to RhoGEF3.

                        (FlyBase Genome Annotators, 2008)

                        Source for identity of RhoGEF3 CG1225 was sequence comparison ( date:001007 ).

                        (FlyBase, 1996-)
                        Nomenclature History
                        Source for database identify of

                        Source for identity of: RhoGEF3 CG1225

                        (FlyBase, 1996-)
                        Nomenclature comments
                        Etymology
                        Synonyms and Secondary IDs (12)
                        Reported As
                        Symbol Synonym
                        CG1225
                        (Álvarez-Fernández et al., 2015, Katzenberger et al., 2012, Greenberg and Hatini, 2011, Zúñiga et al., 2009, Valanne et al., 2007, Asha et al., 2003, Giot et al., 2003)
                        CG13881
                        (Keleman et al., 2009.8.5, Englund et al., 2006, Matyash et al., 2004)
                        CG13882
                        (Keleman et al., 2009.8.5, Zhang et al., 2007, Englund et al., 2006)
                        CG13883
                        (Chen et al., 2012, Zhang et al., 2010, Ferjoux et al., 2007, Zhang et al., 2007, Englund et al., 2006, Giot et al., 2003, Brill, 2000.8.14)
                        CG42377
                        (Kwon et al., 2013, Laslo et al., 2013, Wasbrough et al., 2010, FlyBase Genome Annotators, 2008)
                        CG42378
                        (Howell et al., 2012, FlyBase Genome Annotators, 2008, Ni et al., 2008)
                        CG43976
                        (Bourquard et al., 2023, FlyBase Genome Annotators, 2012, Ni et al., 2010.12.1)
                        DrhoGEF3
                        (Hicks et al., 2001)
                        RhoGEF
                        (Sailem et al., 2014, Brill, 2000.8.14)
                        RhoGEF3
                        (Durel et al., 2025, Chvilicek et al., 2024, Collins et al., 2024, Nakamura and Parkhurst, 2024, Burghardt et al., 2023, di Pietro et al., 2023, Ding et al., 2022, Boucher et al., 2019, FlyBase Genome Annotators, 2019-, Couturier et al., 2017, Nakamura et al., 2017, Clandinin and Owens, 2016-, Gene Disruption Project members, 2015-, Bonnay et al., 2014, Sen et al., 2013, Busser et al., 2012, Howell et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Greenberg and Hatini, 2011, Zhang et al., 2011, Bakal et al., 2007, Bakal et al., 2007, Magalhaes et al., 2007, Wolfgang et al., 2007, Zeitlinger et al., 2007, Englund et al., 2006, Kim et al., 2005)
                        rhoGEF3
                        (Zúñiga et al., 2009)
                        Name Synonyms
                        CG13881
                        CG13883
                        Rho guanine nucleotide exchange factor 3
                        RhoGEF3
                        Secondary FlyBase IDs
                        • FBgn0259743
                        • FBgn0035128
                        • FBgn0259724
                        • FBgn0035127
                        • FBgn0035126
                        • FBgn0259744
                        • FBan0013882
                        • FBgn0259723
                        • FBgn0035125
                        Datasets (0)
                        Study focus (0)
                        Experimental Role
                        Project
                        Project Type
                        Title
                        Study result (0)
                        Result
                        Result Type
                        Title
                        External Crossreferences and Linkouts ( 104 )
                        Sequence Crossreferences
                        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                        Other crossreferences
                        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                        InterPro - A database of protein families, domains and functional sites
                        KEGG Genes - Molecular building blocks of life in the genomic space.
                        MARRVEL_MODEL - MARRVEL (model organism gene)
                        Linkouts
                        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                        DroID - A comprehensive database of gene and protein interactions.
                        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                        FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                        References (90)