iab-8, msa, male specific abdominal, iab-8 ncRNA
represses the transcription of genes located at its 3' end by a sequence-independent, transcriptional interference mechanism - its repressive activity is limited to the CNS, where, in wild-type embryos, it acts on the Hox gene, abd-A, located immediately downstream of it - a lncRNA, called male-specific abdominal, is required for the development of the secondary cells of the Drosophila male accessory gland
Please see the JBrowse view of Dmel\lncRNA:iab8 for information on other features
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Rare iab-8 transcripts (0.2 percent, not annotated) may include downstream abd-A exons, as described in FBrf0218472.
Probable lncRNA gene; may encode small polypeptide(s).
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.48
The primary (unspliced) transcript of this non-coding RNA contains one or more miRNA loci within intronic regions.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lncRNA:iab8 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
iab-4 is expressed in abdominal segment 8 and in more posterior segmental rudiments. It persists in the ventral nerve cord in later stages.
JBrowse - Visual display of RNA-Seq signals
View Dmel\lncRNA:iab8 in JBrowse3-59
3-58.9
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
'msa' ('male-specific abdominal') is the name given to a specific transcript of the lncRNA:iab8 gene (corresponding to lncRNA:iab8-RA and/or lncRNA:iab8-RB) that is expressed exclusively in the adult male abdomen (male accessory gland). One of its introns contains the precursor of mir-iab-8.
The spliced product of the iab-8 RNA is non-coding by traditional criteria, but the possibility of small peptides cannot be rules out. In particular, exon 8 includes a potential 9 amino acid peptide, with appropriate translation and termination signals, and the coding potential for this peptide is well conserved in D. ananassae, D. pseudoobscura and D. willistoni.
The iab-8 ncRNA represses the expression of the abd-A gene in the posterior central nervous system. This repression is accomplished by two redundant mechanisms: firstly via the mir-iab-8 miRNA produced from the iab-8 transcript and secondly by a mechanism that acts only "in cis". The most likely explanation for second mechanism is that iab-8 transcription interferes with the abd-A promoter, which lies just downstream of the iab-8 poly(A) site.
New annotation (CR43261) in release 5.34 of the genome annotation.
Annotation CG31270 restored in release 5.26 of the genome annotation.
Annotation (CG31270) eliminated in release 5.2 of the genome annotation.
New annotation (CG31270) in release 3 of the genome annotation.
Source for merge of: msa CG31270
Source for merge of: iab-8 msa
In addition to producing a miRNA (mir-iab-8) which represses abd-A expression, the 92kb iab-8 non-coding RNA has a second repressive function on abd-A expression which acts in cis, probably due to iab-8 transcription interfering with the abd-A promoter, which lies just downstream of the iab-8 poly(A) site.
Annotation CG31270 merged with annotation CR43261 in release 5.41 of the genome annotation; CG31270 corresponds to the 5' end of several isoforms of CR43261.
Source for identity of: msa CR43261
The "iab-8" non-coding transcript encodes a microRNA (represented by "mir-iab-8") derived from a hairpin which is complementary to the hairpin present in the "iab-4" non-coding transcript (the hairpin in the "iab-4" transcript is represented by "mir-iab-4").
Source for identity of: lncRNA:iab8 iab-8
