PABPC1, dPABP, polyA-binding protein, l(2)k10109, l(2)SH1908
RNA-binding protein involved in translational regulation and nonsense-mediated mRNA decay - interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets
Gene model reviewed during 5.44
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.50
3.8 (northern blot)
Core component of the neuromuscular Atx2 complex, composed of at least Atx2, tyf, pAbp, Lsm12a (PubMed:28388438). Interacts with Atx2 (via PAM2 motif) (PubMed:28388438). Atx2 and pAbp form a subcomplex that can associate with the 5' cap of pre-mRNAs independently of tyf, Lsm12a or me31B (PubMed:28388438). Forms a ribonucleoprotein complex (RNP) containing at least me31B, eIF4E1, cup, tral and pAbp; this interaction is required for the translational silencing of maternal mRNAs during the maternal-to-zygotic transition (PubMed:28875934). In 1-5 hour embryos, interaction with me31B is severely reduced and there is no detected interaction with cup and tral, however interaction with eIF4E1 and eIF4G1 remains unchanged (PubMed:28875934). Interacts with gw; this interaction interferes with the binding of pAbp to eIF4G and is required for miRNA-mediated silencing (PubMed:19797087). Interacts with larp (PubMed:19631203).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pAbp using the Feature Mapper tool.
Comment: anterior compartment only
Comment: anterior compartment
pAbp expression at embryonic stages 1-4 is localized to the posterior pole of the embryo.
pAbp transcript is expressed in cells immediately dorsal to and ventral to the dorsal/ventral compartment boundary of the wing disc; expression is limited to the anterior compartment. No expression is observed in the haltere disc.
GBrowse - Visual display of RNA-Seq signalsView Dmel\pAbp in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: pAbp CG5119
Source for merge of: pAbp anon- EST:Posey247 anon- EST:Posey249
Source for merge of: pAbp l(2)k10109
Source for merge of: pAbp l(2)SH1908
Source for merge of: pAbp BcDNA:LD24412
Source for merge of: CG16803 anon- EST:fe2E5
Source for merge of: pAbp CG16803
Annotation CG5119 and previously withdrawn annotation CG16803 merged in release 5.49 of the genome annotation; CG16803 corresponds to the 3' UTR of CG5119. Merge based on RNA-Seq expression data.
Source for merge of pAbp BcDNA:LD24412 was a shared cDNA ( date:020730 ).
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
pAbp is required to connect the centrosome to the minus-ends of free microtubules.
Annotation CG16803 eliminated in the July 2000 Celera release of annotated sequence.
A sequence comparison and numerical analysis of the RRM-containing (RNA recognition motif) proteins suggests that functionally related RRM-containing proteins have significant sequence similarities in their RRMs.
Isolated from a 3-12 hour embryo cDNA library using a human PABP cDNA probe.