FB2021_01, released Feb 18, 2021

Gene: Dmel\PDZ-GEF

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General Information
Symbol
Dmel\PDZ-GEF
Species
D. melanogaster
Name
PDZ domain-containing guanine nucleotide exchange factor
Annotation Symbol
CG9491
Feature Type
protein_coding_gene
FlyBase ID
FBgn0265778
Gene Model Status
Current
Stock Availability
9 publicly available
Gene Snapshot
PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF) encodes a guanine nucleotide exchange factor for the product of Rap1. The product of PDZ-GEF contributes to ventral furrow formation, border cell migration, macrophage migration, epithelial migration and morphogenesis, eye development, ovary development, spermathecae formation, germline stem cell maintenance in the testis, adherens junction formation, and anchorage of stem cells to niche. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As

Gef26, dizzy, dPDZ-GEF, GEF, l(2)k13720

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:6,323,767..6,332,789 [+]
Recombination map

2-19

RefSeq locus
NT_033779 REGION:6323767..6332789
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (21 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables small GTPase binding
inferred from physical interaction with FLYBASE:Rap1; FB:FBgn0004636
(Boettner and Van Aelst, 2007)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables guanyl-nucleotide exchange factor activity
inferred from sequence or structural similarity with HGNC:16854
(FlyBase Curators, 2008--)
Biological Process (16 terms)
Terms Based on Experimental Evidence (14 terms)
CV Term
Evidence
References
involved_in adherens junction assembly
inferred from genetic interaction with FLYBASE:Rap1; FB:FBgn0004636
(Wang et al., 2006)
involved_in compound eye development
inferred from mutant phenotype
(Huelsmann et al., 2006)
involved_in dorsal closure
inferred from mutant phenotype
(Boettner and Van Aelst, 2007)
involved_in germ-line stem cell population maintenance
inferred from mutant phenotype
(Wang et al., 2006)
involved_in hemocyte migration
inferred from mutant phenotype
(Siekhaus et al., 2010, Huelsmann et al., 2006)
involved_in imaginal disc-derived male genitalia development
inferred from mutant phenotype
(Huelsmann et al., 2006)
involved_in imaginal disc-derived wing morphogenesis
inferred from mutant phenotype
(Huelsmann et al., 2006)
involved_in MAPK cascade
inferred from genetic interaction with FLYBASE:Raf; FB:FBgn0003079
(Lee et al., 2002)
inferred from genetic interaction with FLYBASE:rl; FB:FBgn0003256
(Lee et al., 2002)
inferred from genetic interaction with FLYBASE:Dsor1; FB:FBgn0010269
(Lee et al., 2002)
involved_in oogenesis
inferred from mutant phenotype
(Wang et al., 2006, Lee et al., 2002)
involved_in positive regulation of sevenless signaling pathway
inferred from genetic interaction with FLYBASE:cnk; FB:FBgn0286070
(Baril et al., 2014)
involved_in R7 cell fate commitment
inferred from mutant phenotype
(Baril et al., 2014)
involved_in regulation of compound eye photoreceptor development
inferred from mutant phenotype
(Lee et al., 2002)
involved_in regulation of embryonic cell shape
inferred from mutant phenotype
(Huelsmann et al., 2006)
involved_in substrate-dependent cell migration, cell extension
inferred from mutant phenotype
(Huelsmann et al., 2006)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in positive regulation of GTPase activity
inferred from sequence or structural similarity with HGNC:16854
(FlyBase Curators, 2008--)
involved_in small GTPase mediated signal transduction
inferred from electronic annotation with InterPro:IPR001895, InterPro:IPR019804
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
colocalizes_with adherens junction
inferred from direct assay
(Boettner and Van Aelst, 2007)
located_in apicolateral plasma membrane
inferred from direct assay
(Boettner and Van Aelst, 2007)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in intracellular anatomical structure
inferred from electronic annotation with InterPro:IPR008937
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
RAS GUANINE NUCLEOTIDE EXCHANGE FACTORS -
RasGEFs are Guanine Nucleotide Exchange Factors (GEFs) for Ras family GTPases. The RasGEFs are characterized by a CDC25 homology domain. (Adapted from FBrf0220530).
Pathway (FlyBase)
Positive Regulators of Sevenless Signaling Pathway -
Positive regulators of Sevenless signaling up-regulate the pathway, promoting cell fate to that of an R7 photoreceptor. (Adapted from FBrf0127283 and FBrf0221727).
Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\PDZ-GEF for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene
gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
PDZ-GEF-RA
FBtr0079249
NM_135168
6067
1573
PDZ-GEF-RB
FBtr0300038
NM_001144325
5912
1569
PDZ-GEF-RC
FBtr0300039
NM_001144326
5471
1422
PDZ-GEF-RD
FBtr0333242
NM_001273197
6823
1569
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
PDZ-GEF-PA
FBpp0078879
171.7
1573
7.48
NP_609012
AAF52365
PDZ-GEF-PB
FBpp0289315
171.1
1569
7.37
NP_001137797
ACL83003
PDZ-GEF-PC
FBpp0289316
155.0
1422
7.25
NP_001137798
ACL83004
PDZ-GEF-PD
FBpp0305443
171.1
1569
7.37
NP_001260126
AGB92662
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1569 aa isoforms: PDZ-GEF-PB, PDZ-GEF-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\PDZ-GEF using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
ovary
(Singh et al., 2006)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
spermathecal duct
(Singh et al., 2006)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

PDZ-GEF protein is strongly expressed in spermathecal ducts and stalks.

(Singh et al., 2006)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
colocalizes_with adherens junction
inferred from direct assay
(Boettner and Van Aelst, 2007)
located_in apicolateral plasma membrane
inferred from direct assay
(Boettner and Van Aelst, 2007)
Expression Deduced from Reporters
Reporter: P{PDZ-GEF-GAL4::VP16}
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 13 -- 15
ectoderm
(Boettner and Van Aelst, 2007)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\PDZ-GEF in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 25 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of PDZ-GEF
Transgenic constructs containing regulatory region of PDZ-GEF
Aberrations (Deficiencies and Duplications) ( 8 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4da.G32
lethal | recessive
lethal | recessive | heat sensitive
partially lethal
partially lethal (with PDZ-GEF3)
partially lethal (with PDZ-GEF4)
partially lethal (with PDZ-GEF5)
partially lethal (with PDZ-GEF6)
partially lethal (with PDZ-GEFEP388)
partially lethal (with PDZ-GEFk13720)
partially lethal - majority die (with Df(2L)BSC5)
partially lethal - majority die | recessive
viable
viable, with Scer\GAL4- :
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female sterile | recessive
fertile
sterile (with PDZ-GEF3)
sterile (with PDZ-GEF4)
sterile (with PDZ-GEF5)
sterile (with PDZ-GEF6)
sterile | recessive
Other Phenotypes
Allele
abnormal cell adhesion | oogenesis, with Scer\GAL4slbo.2.6
abnormal cell migration | oogenesis | oogenesis, with Scer\GAL4slbo.2.6
abnormal cell shape, with Scer\GAL4srp
abnormal planar polarity
abnormal planar polarity | somatic clone
abnormal size | adult stage
decreased cell number | spermatogenesis (with PDZ-GEF3)
decreased cell number | spermatogenesis (with PDZ-GEF4)
decreased cell number | spermatogenesis (with PDZ-GEF5)
decreased cell number | spermatogenesis (with PDZ-GEF6)
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
visible
visible, with Scer\GAL4GMR.PF
visible, with Scer\GAL4hs.2sev
visible, with Scer\GAL4pnr-MD237
visible (with PDZ-GEF3)
visible (with PDZ-GEF4)
visible (with PDZ-GEF5)
visible (with PDZ-GEF6)
visible (with PDZ-GEFEP388)
visible (with PDZ-GEFEP388), with Scer\GAL4- :
visible (with PDZ-GEFk13720)
visible (with PDZ-GEFk13720), with Scer\GAL4- :
visible | recessive
visible | somatic clone
Phenotype manifest in
Allele
abdominal segment motor neuron | third instar larval stage, with Scer\GAL4Toll-6-D42
axon | third instar larval stage, with Scer\GAL4Toll-6-D42
border follicle cell, with Scer\GAL4slbo.2.6
cell-cell adherens junction & hub cell (with PDZ-GEF3)
cell-cell adherens junction & hub cell (with PDZ-GEF4)
cyst progenitor cell (with PDZ-GEF3)
cyst progenitor cell (with PDZ-GEF4)
embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4Toll-6-D42
eye
eye, with Scer\GAL4GMR.PF
eye, with Scer\GAL4hs.2sev
eye (with Df(2L)BSC5)
eye (with PDZ-GEF3)
eye (with PDZ-GEF4)
eye (with PDZ-GEF5)
eye (with PDZ-GEF6)
eye (with PDZ-GEFEP388)
eye (with PDZ-GEFEP388), with Scer\GAL4- :
eye (with PDZ-GEFk13720)
eye (with PDZ-GEFk13720), with Scer\GAL4- :
eye | somatic clone
eye photoreceptor cell
eye photoreceptor cell (with PDZ-GEFEP388), with Scer\GAL4- :
eye photoreceptor cell (with PDZ-GEFk13720), with Scer\GAL4- :
eye photoreceptor cell | ectopic, with Scer\GAL4GMR.PF
eye photoreceptor cell | somatic clone
germinal proliferation center (with PDZ-GEF3)
germinal proliferation center (with PDZ-GEF4)
hemocyte | embryonic stage
hemocyte | embryonic stage (with Df(2L)BSC185)
hub cell (with PDZ-GEF3)
hub cell (with PDZ-GEF4)
macrophage
macrophage, with Scer\GAL4srp
male genitalia
male genitalia (with Df(2L)BSC5)
male germline stem cell (with PDZ-GEF3)
male germline stem cell (with PDZ-GEF4)
male germline stem cell (with PDZ-GEF5)
male germline stem cell (with PDZ-GEF6)
male germline stem cell | third instar larval stage (with PDZ-GEF3)
male germline stem cell | third instar larval stage (with PDZ-GEF4)
microtubule | third instar larval stage, with Scer\GAL4Toll-6-D42
ommatidium
ommatidium, with Scer\GAL4GMR.PF
ommatidium | somatic clone
outer photoreceptor cell
outer photoreceptor cell (with Df(2L)BSC5)
ovariole
ovary
photoreceptor cell R7
photoreceptor cell R7 (with Df(2L)BSC5)
photoreceptor cell R7 | somatic clone, with Scer\GAL4Act5C.PP
presumptive embryonic/larval nervous system, with Scer\GAL4srp
spermathecum | increased number
spermathecum | increased number (with PDZ-GEF3)
spermathecum | increased number (with PDZ-GEF4)
spermathecum | increased number (with PDZ-GEF5)
spermathecum | increased number (with PDZ-GEF6)
trichogen cell, with Scer\GAL4pnr-MD237
ventral nerve cord
ventral nerve cord, with Scer\GAL4srp
wing
wing (with PDZ-GEF3)
wing (with PDZ-GEF4)
wing (with PDZ-GEF5)
wing (with PDZ-GEF6)
wing (with PDZ-GEFEP388)
wing (with PDZ-GEFk13720)
wing blade
wing blade (with Df(2L)BSC5)
wing margin
wing vein | ectopic
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (21)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\RAPGEF2
12 of 15
Yes
Yes
2  
Hsap\RAPGEF6
12 of 15
Yes
Yes
Hsap\RALGDS
2 of 15
No
No
Hsap\RAPGEF3
2 of 15
No
No
3  
Hsap\RAPGEF5
2 of 15
No
No
Hsap\RAPGEFL1
2 of 15
No
No
Hsap\RASGRF2
2 of 15
No
Yes
Hsap\RGL2
2 of 15
No
No
Hsap\RGL3
2 of 15
No
No
Hsap\RGL4
2 of 15
No
No
Hsap\SOS1
2 of 15
No
No
Hsap\RAPGEF1
1 of 15
No
No
Hsap\RAPGEF4
1 of 15
No
No
1  
Hsap\RASGEF1A
1 of 15
No
No
Hsap\RASGEF1C
1 of 15
No
No
1  
Hsap\RASGRF1
1 of 15
No
No
Hsap\RASGRP1
1 of 15
No
No
Hsap\RASGRP2
1 of 15
No
No
Hsap\RASGRP4
1 of 15
No
No
Hsap\RGL1
1 of 15
No
No
Hsap\SOS2
1 of 15
No
No
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (36)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Rapgef6
12 of 15
Yes
Yes
Mmus\Rapgef2
8 of 15
No
Yes
Mmus\1700001J03Rik
2 of 15
No
Yes
Mmus\1700123K08Rik
2 of 15
No
Yes
Mmus\4930449I24Rik
2 of 15
No
Yes
Mmus\4930474N05Rik
2 of 15
No
Yes
Mmus\4930596D02Rik
2 of 15
No
Yes
Mmus\4933421I07Rik
2 of 15
No
Yes
Mmus\Gm3402
2 of 15
No
Yes
Mmus\Gm3404
2 of 15
No
Yes
Mmus\Gm3415
2 of 15
No
Yes
Mmus\Gm6309
2 of 15
No
Yes
Mmus\Gm6370
2 of 15
No
Yes
Mmus\Gm6408
2 of 15
No
Yes
Mmus\Ralgds
2 of 15
No
No
Mmus\Rapgef3
2 of 15
No
No
Mmus\Rapgef5
2 of 15
No
No
Mmus\Rapgefl1
2 of 15
No
No
Mmus\Rasgrf2
2 of 15
No
Yes
Mmus\Rgl2
2 of 15
No
No
Mmus\Rgl3
2 of 15
No
No
Mmus\Sos1
2 of 15
No
No
Mmus\1700018F24Rik
1 of 15
No
No
Mmus\Gm3409
1 of 15
No
Yes
Mmus\Gm4871
1 of 15
No
No
Mmus\Gm5565
1 of 15
No
Yes
Mmus\Rapgef1
1 of 15
No
No
Mmus\Rapgef4
1 of 15
No
No
Mmus\Rasgef1a
1 of 15
No
No
Mmus\Rasgef1c
1 of 15
No
No
Mmus\Rasgrf1
1 of 15
No
No
Mmus\Rasgrp1
1 of 15
No
No
Mmus\Rasgrp2
1 of 15
No
No
Mmus\Rasgrp4
1 of 15
No
No
Mmus\Rgl1
1 of 15
No
No
Mmus\Sos2
1 of 15
No
No
Rattus norvegicus (Norway rat) (22)
Rnor\Rapgef2
9 of 13
Yes
Yes
Rnor\Rapgef6
8 of 13
No
Yes
Rnor\LOC102552619
2 of 13
No
Yes
Rnor\Ralgds
2 of 13
No
No
Rnor\Rapgef5
2 of 13
No
No
Rnor\RGD1561017
2 of 13
No
Yes
Rnor\RGD1561114
2 of 13
No
Yes
Rnor\RGD1564129
2 of 13
No
Yes
Rnor\Rgl2
2 of 13
No
No
Rnor\Rgl3
2 of 13
No
No
Rnor\Rapgef3
1 of 13
No
No
Rnor\Rapgef4
1 of 13
No
No
Rnor\Rasgef1a
1 of 13
No
No
Rnor\Rasgef1c
1 of 13
No
No
Rnor\Rasgrp1
1 of 13
No
No
Rnor\Rasgrp2
1 of 13
No
No
Rnor\Rasgrp4
1 of 13
No
No
Rnor\RGD1559508
1 of 13
No
Yes
Rnor\RGD1559804
1 of 13
No
Yes
Rnor\RGD1562508
1 of 13
No
Yes
Rnor\Rgl1
1 of 13
No
No
Rnor\Sos1
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (13)
Xtro\ralgds
2 of 12
Yes
No
Xtro\rapgef2
2 of 12
Yes
Yes
Xtro\rapgef3
2 of 12
Yes
No
Xtro\rapgef5
2 of 12
Yes
No
Xtro\rapgef6
2 of 12
Yes
Yes
Xtro\rapgefl1
2 of 12
Yes
No
Xtro\rgl3
2 of 12
Yes
No
Xtro\zbtb22
2 of 12
Yes
No
Xtro\rapgef4
1 of 12
No
No
Xtro\rasgef1a
1 of 12
No
No
Xtro\rasgrf2
1 of 12
No
No
Xtro\rasgrp1
1 of 12
No
No
Xtro\sos1
1 of 12
No
No
Danio rerio (Zebrafish) (22)
Drer\si:dkey-253i9.4
11 of 15
Yes
Yes
Drer\rapgef2
10 of 15
No
Yes
Drer\rapgef6
6 of 15
No
Yes
Drer\rapgef3
2 of 15
No
No
Drer\rapgef5a
2 of 15
No
No
Drer\rapgefl1
2 of 15
No
No
Drer\rasgrf2b
2 of 15
No
Yes
Drer\rgl2
2 of 15
No
No
Drer\rgl3a
2 of 15
No
No
Drer\si:ch211-251j10.3
2 of 15
No
No
Drer\sos1
2 of 15
No
No
Drer\LOC797658
1 of 15
No
No
Drer\rapgef1a
1 of 15
No
No
Drer\rapgef1b
1 of 15
No
No
Drer\rapgef4
1 of 15
No
No
Drer\rapgef5b
1 of 15
No
No
Drer\rasgrf2a
1 of 15
No
No
Drer\rasgrp2
1 of 15
No
No
Drer\rasgrp4
1 of 15
No
No
Drer\rgl1
1 of 15
No
No
Drer\si:ch211-234p18.3
1 of 15
No
No
Drer\zgc:171558
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (5)
Cele\pxf-1
11 of 15
Yes
Yes
Cele\epac-1
1 of 15
No
No
Cele\rgl-1
1 of 15
No
No
Cele\sos-1
1 of 15
No
No
Cele\Y34B4A.4
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\CDC25
1 of 15
Yes
No
Scer\LTE1
1 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\efc25
1 of 12
Yes
No
Spom\ste6
1 of 12
Yes
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900VP )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00011916
Drosophila simulans
Dsim\GD23402
Drosophila sechellia
Dsec\GM18620
Drosophila erecta
Dere\GG10404
Drosophila yakuba
Dyak\GE13791
Drosophila ananassae
Dana\GF14515
Drosophila pseudoobscura pseudoobscura
Dpse\GA21827
Drosophila persimilis
Dper\GL25520
Drosophila willistoni
Dwil\GK14754
Drosophila virilis
Dvir\GJ12795
Drosophila mojavensis
Dmoj\GI11520
Drosophila grimshawi
Dgri\GH10738
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500CK )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17015666
Glossina morsitans
Tsetse fly
Gmor\GMOY006487
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_12022
Mayetiola destructor
Hessian fly
Mdes\Mdes011271
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL005643
Anopheles darlingi
American malaria mosquito
Adar\ADAC006399
Anopheles gambiae
Malaria mosquito
Agam\AGAP009261
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ005470
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00HY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA004936
Danaus plexippus
Monarch butterfly
Dple\DPOGS202280-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL004725
Apis florea
Little honeybee
Aflo\2212912052
Apis mellifera
Western honey bee
Amel\GB50397
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP11620
Bombus terrestris
Buff-tailed bumblebee
Bter\15216228
Linepithema humile
Argentine ant
Lhum\LH24796
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0030bb
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG010865
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_03712
Tribolium castaneum
Red flour beetle
Tcas\TC003942
Pediculus humanus
Human body louse
Phum\PHUM596850
Rhodnius prolixus
Kissing bug
Rpro\RPRC011857
Cimex lectularius
Bed bug
Clec\CLEC000883
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI003554
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_09377
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00H4 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR007966
Ixodes scapularis
Black-legged tick
Isca\ISCW014925
Stegodyphus mimosarum
African social velvet spider
Smim\KFM61075.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM58016.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur02g13670
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_328339
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_106274
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G00NU )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_016499gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Rapgef2
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Rapgef2_2
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Rapgef2_1
Ciona intestinalis
Vase tunicate
Cint\LOC100176889
Ciona intestinalis
Vase tunicate
Cint\LOC100179213
Gallus gallus
Domestic chicken
Ggal\RAPGEF6
Gallus gallus
Domestic chicken
Ggal\RAPGEF2
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (8)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
C3G
3 of 10
Epac
3 of 10
Rgl
3 of 10
Sos
3 of 10
CG4853
2 of 10
CG5522
2 of 10
CG7369
2 of 10
CG34393
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
RAPGEF2; Rap guanine nucleotide exchange factor 2
12 of 15
609530
 
RAPGEF6; Rap guanine nucleotide exchange factor 6
12 of 15
610499
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser
      View in Interactions Browser
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      PDZ-GEF - Rap1
      two hybrid
      (Boettner and Van Aelst, 2007)
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement
      View in Interactions Browser
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      PDZ-GEF
      suppressible
      Dsor1
      (Lee et al., 2002)
      PDZ-GEF
      suppressible
      Egfr
      (Wang et al., 2006)
      PDZ-GEF
      suppressible
      mys
      (Huelsmann et al., 2006)
      PDZ-GEF
      suppressible
      Raf
      (Lee et al., 2002)
      PDZ-GEF
      suppressible
      Rap1
      (Huelsmann et al., 2006, Wang et al., 2006, Lee et al., 2002)
      PDZ-GEF
      enhanceable
      Rap1
      (Singh et al., 2006)
      PDZ-GEF
      enhanceable
      Rap2l
      (Singh et al., 2006)
      PDZ-GEF
      suppressible
      Ras85D
      (Lee et al., 2002)
      PDZ-GEF
      suppressible
      rl
      (Lee et al., 2002)
      PDZ-GEF
      suppressible
      shg
      (Singh et al., 2006, Wang et al., 2006)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      cnk
      enhanceable
      PDZ-GEF
      (Baril et al., 2014)
      Raf
      enhanceable
      PDZ-GEF
      (Baril et al., 2014)
      External Data
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Positive Regulators of Sevenless Signaling Pathway -
      Positive regulators of Sevenless signaling up-regulate the pathway, promoting cell fate to that of an R7 photoreceptor. (Adapted from FBrf0127283 and FBrf0221727).
      Metabolic Pathways
      External Data
      Linkouts
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2L
      Recombination map

      2-19

      Cytogenetic map
      26C2-26C3
      Sequence location
      2L:6,323,767..6,332,789 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      26C2-26C3
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      26C2-26C3
      (determined by in situ hybridisation)
      (Spradling et al., 1999)
      26C2-26C3
      (BDGP Project Members, 1994-1999)
      Experimentally Determined Recombination Data
      Location

      2-19

      (FlyBase, 2016)

      2-20.4

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (9)
      Genomic Clones (29)
      cDNA Clones (44)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Singh et al., 2006)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: PDZ-GEF Gef26

        (FlyBase, 2009-)

        Source for identity of: Gef26 CG9491

        (FlyBase, 1996-)
        Source for database merge of

        Source for merge of: Gef26 PDZ-GEF

        (FlyBase, 1992-)

        Source for merge of: Gef26 dizzy

        (Edwards, 2003.2.11)

        Source for merge of: Gef26 l(2)k13720

        (Edwards, 2000.3.3)

        Source for merge of: PDZ-GEF GEF

        (FlyBase, 1992-)
        Additional comments

        FlyBase curator comment: Renamed from 'Gef26' to 'PDZ-GEF' because: i) 'Gef26' isn't very informative; ii) There are no recorded publications that use 'Gef26'; iii) the first paper to characterize the gene (FBrf0152217) called the gene 'PDZ-GEF'; iv) 'PDZ-GEF' is descriptive and popular in the literature.

        (FlyBase, 2009-)

        Source for identity of Gef26 CG9491 was sequence comparison ( date:011113 ).

        (FlyBase, 1996-)
        Other Comments

        Gef26 is required for macrophage shape and migration during embryogenesis and depends on the function of the R and mys proteins.

        (Huelsmann et al., 2006)

        Gef26 is necessary for the maintenance (formation?) of adherens junctions which anchor male germline stem cells to the hub in the testis.

        (Wang et al., 2006)

        Plays a role in the development of the spermathecae.

        (Singh et al., 2005)

        Mutant females show supernumerary spermathecae.

        (Singh et al., 2005)

        PDZ-GEF is the first component of a conserved "inside-out" signalling pathway which changes the adhesive properties of macrophages via integrins.

        (Huelsmann et al., 2004)

        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

        (Kiger et al., 2003)

        Retinal axons make abnormal projections to their targets in the lamina in Gef26 mutants, while the photoreceptors appear normal.

        (Bottalico et al., 2002)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 46 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (17)
        Reported As
        Symbol Synonym
        CG9491
        (Keleman et al., 2009.8.5, Perkins et al., 2009, van Dam et al., 2009, Ni et al., 2008, Lee et al., 2002, Stein et al., 2002, Anholt and Mackay, 2001)
        D-PDZGEF
        (Dupuy et al., 2005)
        DGEF
        (Hou et al., 2005, Singh et al., 2005)
        Dzy
        (Sun et al., 2020, Spahn et al., 2012)
        EC2-8
        (Baril et al., 2014)
        GEF
        (Edwards et al., 2003)
        Gef26
        (Heo et al., 2018, Dorn and Dorn, 2015, Baril et al., 2014, Wang et al., 2013, Aranjuez et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Diez et al., 2011, Friedman et al., 2011, Michel et al., 2011, van Dam et al., 2009, Christensen et al., 2008.10.20, Bakal et al., 2007, Bakal et al., 2007, Boettner and Van Aelst, 2007, Christensen and Cook, 2006.8.29, Christensen and Cook, 2006.8.29, Singh et al., 2006, Wang et al., 2006)
        PDF-GEF Dizzy
        (Marchese et al., 2007)
        PDZ-GEF
        (Meltzer et al., 2019, Ray et al., 2019, Raza et al., 2019, Sawant et al., 2018, Baril et al., 2014, Zhang and Therrien, 2008)
        dPDZ-GEF
        (Boettner and Van Aelst, 2007, Huelsmann et al., 2006, Lee et al., 2002)
        dizzy
        (Schmidt et al., 2018, Wang et al., 2013, Michel et al., 2011, Siekhaus et al., 2010, Huelsmann et al., 2006, Marchese et al., 2005, Bottalico, 2001.10.15)
        dzy
        (Bonello et al., 2018, Ott et al., 2008)
        gef26
        (Ou et al., 2019, Heo et al., 2017)
        l(2)k13720
        (Spradling et al., 1999, BDGP Project Members, 1994-1999)
        Name Synonyms
        Dizzy
        (Gilmour et al., 2017, Parsons and Foley, 2016, Vlisidou and Wood, 2015, Wang et al., 2014, Spahn et al., 2012, Ott et al., 2008)
        GEF
        Gef26
        PDZ domain-containing guanine nucleotide exchange factor
        PDZ-GEF
        PDZ-GEF/Dizzy
        (Zhang and Therrien, 2008)
        dizzy
        (Marchese et al., 2007)
        Secondary FlyBase IDs
        • FBgn0069356
        • FBgn0031798
        • FBgn0021873
        • FBgn0265624
        • FBgn0082671
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (89)