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General Information
Symbol
Dmel\Zasp52
Species
D. melanogaster
Name
Z band alternatively spliced PDZ-motif protein 52
Annotation Symbol
CG30084
Feature Type
FlyBase ID
FBgn0265991
Gene Model Status
Stock Availability
Gene Summary
Z band alternatively spliced PDZ-motif protein 52 (Zasp52) encodes a member of the Zasp protein family. It is a scaffold protein that binds the product of Actn and localizes to muscle attachment sites and Z-discs in muscle cells, as well as localizing cortically in non-muscle cells. It plays a role in muscle development, especially in myofibril assembly. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)
Also Known As

Zasp, Z(210), Z band alternatively spliced PDZ-motif protein, zetalin

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:15,762,565..15,817,004 [-]
Recombination map
2-75
RefSeq locus
NT_033778 REGION:15762565..15817004
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (19 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from physical interaction with UniProtKB:P18091
(assigned by UniProt )
inferred from physical interaction with FLYBASE:Actn; FB:FBgn0000667
inferred from mutant phenotype
(assigned by UniProt )
inferred from physical interaction with FLYBASE:Actn; FB:FBgn0000667
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001198141
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001198141
(assigned by GO_Central )
Biological Process (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from mutant phenotype
involved_in myofibril assembly
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001198141
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001198141
(assigned by GO_Central )
Cellular Component (12 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
located_in focal adhesion
inferred from direct assay
inferred from direct assay
located_in stress fiber
inferred from direct assay
located_in zonula adherens
inferred from direct assay
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
is_active_in adherens junction
inferred from biological aspect of ancestor with PANTHER:PTN001198141
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001198141
(assigned by GO_Central )
is_active_in stress fiber
inferred from biological aspect of ancestor with PANTHER:PTN001198141
(assigned by GO_Central )
is_active_in Z disc
inferred from biological aspect of ancestor with PANTHER:PTN001198150
(assigned by GO_Central )
Gene Group (FlyBase)
Protein Family (UniProt)
-
Summaries
Gene Snapshot
Z band alternatively spliced PDZ-motif protein 52 (Zasp52) encodes a member of the Zasp protein family. It is a scaffold protein that binds the product of Actn and localizes to muscle attachment sites and Z-discs in muscle cells, as well as localizing cortically in non-muscle cells. It plays a role in muscle development, especially in myofibril assembly. [Date last reviewed: 2019-03-21]
Protein Function (UniProtKB)
Regulator of cell matrix adhesion having two related functions, one upstream of Actn organizing the Z line and the other downstream of integrins regulating assembly of integrin adhesion sites. Also required for the formation of myotendinous junctions in muscles.
(UniProt, A1ZA47)
Gene Model and Products
Number of Transcripts
22
Number of Unique Polypeptides
21

Please see the JBrowse view of Dmel\Zasp52 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 6.04

Gene model reviewed during 5.51

Gene model reviewed during 5.42

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model includes transcripts encoding non-overlapping portions of the full CDS.

Gene model reviewed during 5.44

Gene model reviewed during 5.48

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 6.32

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0479884
999
245
FBtr0087315
4138
1082
FBtr0100387
3176
780
FBtr0100388
7418
2194
FBtr0111048
3506
890
FBtr0301313
4649
1271
FBtr0302163
1726
449
FBtr0310086
3527
897
FBtr0310087
4790
1318
FBtr0310088
4637
1267
FBtr0329909
1963
515
FBtr0329910
951
233
FBtr0329911
6327
1758
FBtr0329912
3002
722
FBtr0329913
3197
787
FBtr0329914
2789
651
FBtr0329915
2984
716
FBtr0329916
3701
955
FBtr0342711
7088
2084
FBtr0392901
7262
2142
FBtr0392902
2241
396
FBtr0392918
1782
449
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0428217
26.5
245
5.68
FBpp0086449
116.7
1082
8.43
FBpp0099800
85.4
780
8.49
FBpp0099801
238.1
2194
6.37
FBpp0110340
96.0
890
8.25
FBpp0290528
136.3
1271
7.70
FBpp0291373
48.8
449
9.02
FBpp0301784
96.3
897
8.53
FBpp0301785
142.0
1318
8.83
FBpp0301786
137.0
1267
8.95
FBpp0302943
55.5
515
7.05
FBpp0302944
25.7
233
7.71
FBpp0302945
191.5
1758
5.72
FBpp0302946
79.4
722
8.77
FBpp0302947
85.7
787
8.77
FBpp0302948
70.9
651
8.53
FBpp0302949
77.2
716
8.53
FBpp0302950
102.2
955
8.25
FBpp0309588
227.5
2084
6.53
FBpp0352243
233.4
2142
6.36
FBpp0352244
43.5
396
8.67
FBpp0352256
48.8
449
9.02
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

449 aa isoforms: Zasp52-PK, Zasp52-PZ
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with alpha-actinin (Actn).

(UniProt, A1ZA47)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Zasp52 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Zasp52 was immunostained by using the Z(210) antibody. Zasp52 was found on the Z discs on the myofibrils of internal flight muscles, but not on the myofibrils of the nearby mesothoracic extracoxal depressor muscle 66 (TDT muscle).

At 30 h APF, Zasp52 shows a punctate distribution in the forming myofibrils. At 48 h APF, Zasp52 appears as broad dots at the forming Z-disc. At 72 h APF, Zasp52 is found in evenly spaced stripes at the Z-disc. In the adult, the myofibrils have grown in width and Zasp52 labels the striations. Zasp52 localizes with Actn.

A Zasp52 antibody that recognizes all isoforms is expressed in third instar larval body wall muscle and midgut, as well as in adult indirect flight muscle (IFM), tubular jump muscle (TDT), abdominal muscles, heart muscle, and midgut. Zasp52 localizes to Z-disks in all muscle tissues, heart, visceral, and somatic. Antibodies against exon 8 sequences (encoding one form of the variable LIM1 domain) shows tissue-specific expression. It detects expression in adult heart and certain somatic muscles (IFM and TDT), but not in larval and adult midgut, larval body wall muscles and abdominal muscles.

Z(210) protein is found only in the indirect flight muscle (IFM) and large cells of mesothoracic extracoxal depressor muscle 66 (tergal depressor of trochanter, or jump muscle). It is first detected in the precursors of these cells at 40 hr APF.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
located_in focal adhesion
inferred from direct assay
inferred from direct assay
located_in stress fiber
inferred from direct assay
located_in zonula adherens
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Zasp52 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
EMBL-EBI Single Cell Expression Atlas
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 21 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 18 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Zasp52
Transgenic constructs containing regulatory region of Zasp52
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (33)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
8 of 15
Yes
Yes
6 of 15
No
Yes
4 of 15
No
Yes
3 of 15
No
No
1  
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
2  
1 of 15
No
No
2  
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (22)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
7 of 15
No
Yes
6 of 15
No
Yes
4 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
3  
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (13)
6 of 13
Yes
Yes
6 of 13
Yes
Yes
3 of 13
No
Yes
3 of 13
No
Yes
3 of 13
No
Yes
2 of 13
No
No
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
No
2 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (16)
7 of 12
Yes
Yes
5 of 12
No
Yes
3 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (28)
5 of 15
Yes
Yes
5 of 15
Yes
Yes
5 of 15
Yes
Yes
4 of 15
No
Yes
4 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (14)
8 of 15
Yes
Yes
3 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (4)
3 of 15
Yes
Yes
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (3)
3 of 12
Yes
No
2 of 12
No
No
1 of 12
No
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919011O )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500A3 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03PO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Tribolium castaneum
Red flour beetle
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03MP )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Stegodyphus mimosarum
African social velvet spider
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (17)
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 3 )
Modifiers Based on Experimental Evidence ( 2 )
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Interacts with alpha-actinin (Actn).
(UniProt, A1ZA47 )
Linkouts
DroID - A comprehensive database of gene and protein interactions.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-75
Cytogenetic map
Sequence location
2R:15,762,565..15,817,004 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
52C4-52C7
Limits computationally determined from genome sequence between P{lacW}Vha36k07207 and P{lacW}l(2)k05713k05713
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (17)
Genomic Clones (33)
cDNA Clones (92)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
Commercially Available Antibodies
 
Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: Zasp52 Zasp

Source for identity of: Zasp CG30084

Source for database merge of

Source for merge of: CG8242 CG12968

Source for merge of: CG12969 CG8242

Source for merge of: Zasp52 Z(210)

Additional comments

Annotations CG12969 and CG8242 merged as CG30084 in release 3 of the genome annotation.

FlyBase curator comment: "tun" was stated to correspond to "CG12969" in FBrf0159552, however, flanking sequence of the P{GAL4}tun1 insertion indicates that "tun" actually corresponds to the nearby CG8253 annotation (see FBrf0188553).

FBrf0100232 discusses 'zetalin' but subsequent work revealed that the gene corresponding to cDNA with accession number U51473 is actually "Strn-Mlck".

Other Comments

Encodes a Z-band protein expressed in flight muscle.

A protein of the Z-bands of asynchronous muscles and of the large cells of the tergal depressor muscle of trochanter only.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 116 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
EMBL-EBI Single Cell Expression Atlas
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL
Linkouts
DroID - A comprehensive database of gene and protein interactions.
Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (19)
Reported As
Symbol Synonym
CG12968
ZASP52
Zbp210
tun
Name Synonyms
CG30084
Z band alternatively spliced PDZ-motif containing protein 52
Z band alternatively spliced PDZ-motif protein
Z band alternatively spliced PDZ-motif protein 52
Z-band-protein-210
Secondary FlyBase IDs
  • FBgn0083919
  • FBgn0034042
  • FBgn0034043
  • FBgn0034044
  • FBgn0050084
  • FBgn0028659
Datasets (0)
Study focus (0)
Experimental Role
Project
Project Type
Title
References (129)