FB2021_02, released April 13, 2021

Gene: Dmel\SclB

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General Information
Symbol
Dmel\SclB
Species
D. melanogaster
Name
Sarcolamban B
Annotation Symbol
CG45091
Feature Type
protein_coding_gene
FlyBase ID
FBgn0266492
Gene Model Status
Current
Stock Availability
3 publicly available
Gene Summary
Plays an essential role in the regulation of calcium transport at the sarcoplasmic reticulum (SR), which is secondarily required for regular muscle contraction. (UniProt, C0HJH3)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

pncr003:2L , scl, sarcolamban

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:16,837,972..16,841,884 [+]
Recombination map
2-53
RefSeq locus
NT_033779 REGION:16837972..16841884
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (1 term)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in regulation of cardiac muscle contraction
inferred from mutant phenotype
(Magny et al., 2013)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Protein Function (UniProtKB)
    Plays an essential role in the regulation of calcium transport at the sarcoplasmic reticulum (SR), which is secondarily required for regular muscle contraction.
    (UniProt, C0HJH3)
    Gene Model and Products
    Number of Transcripts
    4
    Number of Unique Polypeptides
    1

    Please see the JBrowse view of Dmel\SclB for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Annotated transcripts do not represent all supported alternative splices within 5' UTR.

    Low-frequency RNA-Seq exon junction(s) not annotated.

    Gene model reviewed during 5.54

    Sequence Ontology: Class of Gene
    gene_with_dicistronic_mRNA
    (FlyBase Genome Annotators, 2013, Magny et al., 2013, Savard et al., 2006)
    nuclear_gene
    (FlyBase Genome Annotators, 2013)
    protein_coding_gene
    (Mackowiak et al., 2015, FlyBase Genome Annotators, 2013, Savard et al., 2006)
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    SclB-RA
    FBtr0344539
    NM_001299061
    848
    29
    SclB-RB
    FBtr0344540
    NM_001299062
    992
    29
    SclB-RC
    FBtr0344541
    NM_001299063
    954
    29
    SclB-RD
    FBtr0344542
    NM_001299064
    909
    29
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    SclB-PA
    FBpp0310891
    3.4
    29
    4.26
    NP_001285990
    AHN54504
    SclB-PB
    FBpp0310892
    3.4
    29
    4.26
    NP_001285991
    AHN54505
    SclB-PC
    FBpp0310893
    3.4
    29
    4.26
    NP_001285992
    AHN54506
    SclB-PD
    FBpp0310894
    3.4
    29
    4.26
    NP_001285993
    AHN54507
    Polypeptides with Identical Sequences

    The group(s) of polypeptides indicated below share identical sequence to each other.

    29 aa isoforms: SclB-PA, SclB-PB, SclB-PC, SclB-PD
    Additional Polypeptide Data and Comments
    Reported size (kDa)
    Comments
    External Data
    Subunit Structure (UniProtKB)

    Interacts with SERCA.

    (UniProt, C0HJH3)
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\SclB using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    Polypeptide Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\SclB in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Images
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, Transgenic Constructs, and Aberrations
    Classical and Insertion Alleles ( 3 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 11 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of SclB
    Transgenic constructs containing regulatory region of SclB
    Aberrations (Deficiencies and Duplications) ( 1 )
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Phenotype manifest in
    Allele
    adult heart, with Scer\GAL4Mef2.PR
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Download All OrthoDB Orthologs
    Human Orthologs (via DIOPT v8.0)
    Homo sapiens (Human) (0)
    No records found.
    Model Organism Orthologs (via DIOPT v8.0)
    Mus musculus (laboratory mouse) (0)
    No records found.
    Rattus norvegicus (Norway rat) (0)
    No records found.
    Xenopus tropicalis (Western clawed frog) (0)
    No records found.
    Danio rerio (Zebrafish) (0)
    No records found.
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( None identified )
    No orthologies identified
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )
    No non-Drosophilid orthologies identified
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
    No non-Dipteran orthologies identified
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v8.0)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser
      View in Interactions Browser
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      SclB - SERCA
      anti tag coimmunoprecipitation, western blot
      (Magny et al., 2013)
      Summary of Genetic Interactions
      esyN Network Diagram
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Subunit Structure (UniProtKB)
      Interacts with SERCA.
      (UniProt, C0HJH3 )
      Linkouts
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2L
      Recombination map
      2-53
      Cytogenetic map
      36B2-36B2
      Sequence location
      2L:16,837,972..16,841,884 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      36B2-36B2
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      2-53

      (FlyBase, 2016)

      2-51.3

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (3)
      Genomic Clones (13)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (28)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: BcDNA:RE28911 CR31696

        (FlyBase Genome Annotators, 2002-2003)

        Source for identity of: pncr003:2L CR31696

        (Tupy et al., 2005)
        Source for database merge of

        Source for merge of: CR31696 BEST:LP06756

        (Rana, 2004.7.8)
        Additional comments

        Annotation CR31696 split into CG45090 (corresponds to SclA) and CG45091 (corresponds to SclB) in release 5.54 of the genome annotation. Split based on information in FBrf0195266.

        (FlyBase Genome Annotators, 2013)

        One or more of the processed transcripts for these genes contain two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG45090 (SclA, FBgn0266491), CG45091 (SclB, FBgn0266492).

        (FlyBase Genome Annotators, 2013)

        The SclA and SclB peptides are encoded by a dicistronic transcript.

        (Magny et al., 2013)

        Source for gene model annotation split (release 5.54): CR31696 split into CG45090 and CG45091, which encode two small, similar peptides encoded by non-overlapping reading frames in a dicistronic mRNA.

        (Savard et al., 2006)
        Other Comments

        The SclA and SclB peptides regulate calcium transport in cardiac cells and are required for regular muscle contraction in the heart.

        (Magny et al., 2013)

        New annotation (CR31696) in release 3 of the genome annotation.

        (FlyBase Genome Annotators, 2002-2003)
        Origin and Etymology
        Discoverer
        Etymology

        "SclA" and "SclB" are named "Sarcolamban A" and "Sarcolamban B" based on their similarity and probable homology to both the vertebrate sarcolipin and phospholamban genes.

        (Magny et al., 2013)
        Identification
        External Crossreferences and Linkouts ( 26 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        KEGG Genes - Molecular building blocks of life in the genomic space.
        Linkouts
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Synonyms and Secondary IDs (15)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (22)