FlyBase Gene Report: Dmel\Hsc70-4

General Information

Symbol

Dmel\Hsc70-4

Species

D. melanogaster

Name

Heat shock protein 70 cognate 4

Annotation Symbol

CG4264

Feature Type

protein_coding_gene

FlyBase ID

FBgn0266599

Gene Model Status

Current

Stock Availability

28 publicly available

Gene Summary

Heat shock protein cognate 4 (Hsc70-4) encodes a protein involved in protein folding. It contributes to clathrin-mediated endocytosis, neurotransmitter exocytosis and RNAi response. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Hsc4, Hsc70, Heat shock protein cognate 4, BAP74, Hsp70

Key Links

Genomic Location

Cytogenetic map

88E4-88E4

Sequence location

3R:15,242,687..15,246,614 [+]

Recombination map

3-56

RefSeq locus

NT_033777 REGION:15242687..15246614

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (27 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables ATP-dependent protein folding chaperone

inferred from direct assay

(Uytterhoeven et al., 2015)

enables protein-folding chaperone binding

inferred from direct assay

(Wang and Brock, 2003)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR013126

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from biological aspect of ancestor with PANTHER:PTN000452648

(GO Reference Genome Project, 2011-)

enables ATP-dependent protein folding chaperone

inferred from electronic annotation with InterPro:IPR013126

(InterPro Project Members, 2004-)

enables heat shock protein binding

inferred from biological aspect of ancestor with PANTHER:PTN000452648

(GO Reference Genome Project, 2011-)

enables protein folding chaperone

inferred from biological aspect of ancestor with PANTHER:PTN000452648

(GO Reference Genome Project, 2011-)

Biological Process (15 terms)

Terms Based on Experimental Evidence (12 terms)

CV Term

Evidence

References

involved_in axon guidance

inferred from mutant phenotype

(Schmucker et al., 1997)

involved_in axonal fasciculation

inferred from mutant phenotype

(Schmucker et al., 1997)

involved_in cellular response to topologically incorrect protein

inferred from mutant phenotype

(Jin et al., 2003)

involved_in follicle cell of egg chamber development

inferred from mutant phenotype

(Jia et al., 2015)

involved_in late endosomal microautophagy

inferred from mutant phenotype

(Uytterhoeven et al., 2015)

involved_in membrane bending

inferred from direct assay

(Uytterhoeven et al., 2015)

involved_in nervous system development

inferred from mutant phenotype

(Schmucker et al., 1997)

involved_in neurotransmitter secretion

inferred from mutant phenotype

(Bronk et al., 2001)

inferred from genetic interaction with FLYBASE:Csp; FB:FBgn0004179

(Bronk et al., 2001)

inferred from physical interaction with FLYBASE:Csp; FB:FBgn0004179

(Bronk et al., 2001)

involved_in protein folding

inferred from direct assay

(Uytterhoeven et al., 2015)

involved_in regulatory ncRNA-mediated post-transcriptional gene silencing

inferred from mutant phenotype

(Dorner et al., 2006)

involved_in RISC complex assembly

inferred from genetic interaction with FLYBASE:Hsp83; FB:FBgn0001233,FLYBASE:cPges; FB:FBgn0037728

(Tsuboyama et al., 2018)

involved_in vesicle-mediated transport

inferred from mutant phenotype

(Chang et al., 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in chaperone cofactor-dependent protein refolding

inferred from biological aspect of ancestor with PANTHER:PTN000452648

(GO Reference Genome Project, 2011-)

involved_in mRNA splicing, via spliceosome

inferred by curator from GO:0071011,GO:0071013

(Herold et al., 2009)

involved_in protein refolding

inferred from biological aspect of ancestor with PANTHER:PTN000452648

(GO Reference Genome Project, 2011-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

located_in cytosol

inferred from high throughput direct assay

(Lee et al., 2014)

located_in nucleus

inferred from high throughput direct assay

(Lee et al., 2014)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN002321897

(GO Reference Genome Project, 2011-)

is_active_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN002500132

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002500132

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN002500132

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

HEAT SHOCK PROTEIN 70 CHAPERONES

Protein Family (UniProt)

Belongs to the heat shock protein 70 family. (P11147)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065:

Summaries

Gene Snapshot

Gene Group (FlyBase)

HEAT SHOCK PROTEIN 70 CHAPERONES -: The Heat Shock Protein 70 (Hsp70) superfamily of chaperones assist in numerous folding processes and are upregulated by heat stress and toxic chemicals. Hsp70 chaperones share a highly conserved bipartite domain structure composed of an ATPase domain and a substrate-binding domain. (Adapted from PMID:17441502, FBrf0232269 and FBrf0174945).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Hsc70-4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P11147)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.48

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Hsc70-4-RA

FBtr0083057

NM_079632

2368

651

Hsc70-4-RB

FBtr0083060

NM_169625

2339

651

Hsc70-4-RC

FBtr0083058

NM_169626

2280

651

Hsc70-4-RD

FBtr0083059

NM_169627

2352

651

Hsc70-4-RE

FBtr0083055

NM_176502

2284

651

Hsc70-4-RF

FBtr0083056

NM_176503

2359

651

Hsc70-4-RG

FBtr0337053

NM_001275657

2336

651

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Hsc70-4-PA

FBpp0082516

71.1

651

5.16

FBpp0082519

71.1

651

5.16

FBpp0082517

71.1

651

5.16

FBpp0082518

71.1

651

5.16

FBpp0082514

71.1

651

5.16

FBpp0082515

71.1

651

5.16

FBpp0307982

71.1

651

5.16

P11147

NP_001262586

AGB95967

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

651 aa isoforms: Hsc70-4-PA, Hsc70-4-PB, Hsc70-4-PC, Hsc70-4-PD, Hsc70-4-PE, Hsc70-4-PF, Hsc70-4-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

70 (kD)

(Carbajal et al., 1993)

Comments

External Data

Subunit Structure (UniProtKB)

Associates with the elongator complex; this interaction is required for some functions of the elongator complex but is not required for regulation of microtubule dynamics.

(UniProt, P11147)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hsc70-4 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.47

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Bronk et al., 2001)

third instar larval stage

embryonic/larval neuromuscular junction

• terminal bouton

(Bronk et al., 2001)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

ovary

(Pisa et al., 2009)

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Hsc70-4 protein is expressed in all cells throughout embryonic development.

(Bronk et al., 2001, Carbajal et al., 1993)

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

located_in cytosol

inferred from high throughput direct assay

(Lee et al., 2014)

located_in nucleus

inferred from high throughput direct assay

(Lee et al., 2014)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Expression Deduced from Reporters

Reporter: PBac{803.P.SVS-0}Hsc70-4^CPTI003798

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain

Comment: expression throughout adult brain

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Hsc70-4 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Hsc70-4

View exonic expression by tissue for Dmel\Hsc70-4

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266599

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41840/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266599

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001219

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266599

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4264

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 42 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 23 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Hsc70-4

Transgenic constructs containing regulatory region of Hsc70-4

Aberrations (Deficiencies and Duplications) ( 9 )

Inferred from experimentation ( 9 )

Gene not disrupted in

Df(3R)ea

(Mollaaghababa et al., 2001)

Df(3R)kar^5l

(Mollaaghababa et al., 2001)

Df(3R)sbd105

(Spradling et al., 1999)

Gene disrupted in

Df(3R)Exel7323

(Ryder, 2004.4.26)

Df(3R)Hsc70-4^Δ11

(Bronk et al., 2001)

Df(3R)Hsc70-4^Δ356

(Bronk et al., 2001)

Df(3R)PA2

(Hing et al., 1999)

Df(3R)PB2

(Hing et al., 1999)

Df(3R)PG4

(Hing et al., 1999)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^how-24B

lethal, with Scer\GAL4^nub-AC-62

Hsc70-4^HMJ21529

lethal (with Hsc70-4^54.1)

lethal (with Hsc70-4¹⁹⁵)

Hsc70-4^54.1

lethal (with Hsc70-4⁰³⁵⁵⁰)

Hsc70-4^54.1

lethal (with Hsc70-4^II-R35-R2)

Hsc70-4^54.1

lethal (with Hsc70-4^II-R35-R7)

Hsc70-4^54.1

lethal (with Hsc70-4^I-R35-R7)

Hsc70-4^54.1

lethal (with Hsc70-4^PB2)

Hsc70-4^54.1

lethal | dominant, with Scer\GAL4^unspecified

lethal | female limited, with Scer\GAL4^dome-PG14

Hsc70-4^HMJ21529

lethal | paternal effect, with Scer\GAL4^VP16.bam

Hsc70-4^KK108223

lethal | recessive

lethal - all die before end of larval stage

Hsc70-4^KO

lethal - all die before end of larval stage, with Scer\GAL4^337Y

lethal - all die before end of larval stage, with Scer\GAL4^da.PU

Hsc70-4^GLV21049

lethal - all die before end of larval stage | recessive

lethal - all die before end of pupal stage | recessive

partially lethal - majority die

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Hsc70-4^GD11264

viable

viable, with Scer\GAL4^how-24B

Hsc70-4^UAS.cEa

viable, with Scer\GAL4^tin.CΔ4

viable (with Df(3R)Hsc70-4^Δ356)

Hsc70-4^Wee-P1

Sterility

Allele

female sterile, with Scer\GAL4^VP16.otu

Hsc70-4^HMJ21529

male semi-sterile | progressive

Hsc70-4^Tag:FLAG

Other Phenotypes

Allele

abnormal behavior, with Scer\GAL4^156Y

Hsc70-4^D206S.UAS

abnormal body size | P-stage, with Scer\GAL4^Mef2.PR

Hsc70-4^HMJ21529

abnormal body size | P-stage, with Scer\GAL4^Mef2.PU

Hsc70-4^HMJ21529

abnormal mitotic cell cycle | somatic clone

abnormal neuroanatomy, with Scer\GAL4^Ddc.PL

Hsc70-4^K71S.UAS

abnormal neurophysiology | larval stage

abnormal neurophysiology | larval stage, with Scer\GAL4^elav.PU

abnormal neurophysiology | larval stage (with Df(3R)BSC471)

Hsc70-4^Δ19

abnormal neurophysiology | larval stage (with Hsc70-4^Δ11)

Hsc70-4^Δ19

abnormal neurophysiology | larval stage (with Hsc70-4^Δ19)

abnormal neurophysiology | larval stage (with Hsc70-4^Δ356)

Hsc70-4^Δ19

abnormal size

Hsc70-4^HS1-25

abnormal size | third instar larval stage, with Scer\GAL4^Cg.PA

Hsc70-4^HMS00152

decreased fecundity | male

Hsc70-4^Tag:FLAG

decreased size | spermatogenesis, with Scer\GAL4^VP16.bam

Hsc70-4^KK108223

flightless, with Scer\GAL4^Mef2.PR

Hsc70-4^GD11264

increased cell size, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

Hsc70-4^K71S.UAS

increased cell size | somatic clone

melanotic mass phenotype | recessive

Hsc70-4⁰³⁵⁵⁰

non-enhancer of variegation, with In(1)w^m4h

Hsc70-4^54.1

non-suppressor of variegation, with In(1)w^m4h

Hsc70-4^54.1

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

some die during embryonic stage | maternal effect

Hsc70-4⁰³⁵⁵⁰

some die during larval stage, with Scer\GAL4^337Y

some die during larval stage | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

Hsc70-4^GD11264

some die during pupal stage | recessive

suppressor of variegation | dominant, with P{SUPor-P}KV00108

Hsc70-4^54.1

visible, with Scer\GAL4^pnr-MD237

Hsc70-4^GD11264

visible | adult stage, with Scer\GAL4^GMR.PU

Hsc70-4^KK108223

visible | dominant | cold sensitive

Hsc70-4¹⁹⁵

visible | dominant | homeotic

Hsc70-4^54.1

wild-type

Hsc70-4^+t14

Phenotype manifest in

Allele

abdominal 4 ventral denticle belt

Hsc70-4^54.1

abdominal segment 1 | maternal effect

Hsc70-4⁰³⁵⁵⁰

abdominal segment 2 | maternal effect

Hsc70-4⁰³⁵⁵⁰

abdominal segment 3 | maternal effect

Hsc70-4⁰³⁵⁵⁰

abdominal segment 4 | maternal effect

Hsc70-4⁰³⁵⁵⁰

abdominal segment 5 | maternal effect

Hsc70-4⁰³⁵⁵⁰

abdominal segment 6 | maternal effect

Hsc70-4⁰³⁵⁵⁰

abdominal segment 7 | maternal effect

Hsc70-4⁰³⁵⁵⁰

abdominal segment 8 | maternal effect

Hsc70-4⁰³⁵⁵⁰

abdominal segment 9 | maternal effect

Hsc70-4⁰³⁵⁵⁰

abdominal segment 10 | maternal effect

Hsc70-4⁰³⁵⁵⁰

abdominal segment 11 | maternal effect

Hsc70-4⁰³⁵⁵⁰

adult abdominal segment 4

Hsc70-4^54.1

adult abdominal segment 5 | ectopic

Hsc70-4^54.1

adult abdominal segment 6

Hsc70-4^54.1

adult abdominal segment 7 | ectopic

Hsc70-4^54.1

anterior pupal spiracle | larval stage, with Scer\GAL4^Mef2.PR

Hsc70-4^HMJ21529

axon | larval stage

Bolwig nerve

Hsc70-4⁰³⁵⁵⁰

Bolwig organ

Hsc70-4⁰³⁵⁵⁰

coated vesicle

Hsc70-4^HS1-25

dopaminergic neuron, with Scer\GAL4^Ddc.PL

Hsc70-4^K71S.UAS

embryonic/first instar larval cuticle | maternal effect

Hsc70-4⁰³⁵⁵⁰

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

Hsc70-4^HMS00152

endosome | somatic clone

Hsc70-4^HS1-25

eye

Hsc70-4¹⁹⁵

eye, with Scer\GAL4^GMR.PF

Hsc70-4^K71S.UAS

eye, with Scer\GAL4^GMR.PU

Hsc70-4^KK108223

eye disc

Hsc70-4^HS1-25

garland cell

Hsc70-4^HS1-25

head | anterior | maternal effect

Hsc70-4⁰³⁵⁵⁰

imaginal disc

Hsc70-4^e3

imaginal disc, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

Hsc70-4^K71S.UAS

interommatidial bristle, with Scer\GAL4^GMR.PU

Hsc70-4^KK108223

larval muscle system

larval muscle system, with Scer\GAL4^how-24B

Hsc70-4^D206S.UAS

larval somatic muscle cell | larval stage, with Scer\GAL4^Mef2.PR

Hsc70-4^HMJ21529

lipid droplet | adult stage | progressive, with Scer\GAL4^Mhc.PK

Hsc70-4^RNAi.UAS.cUa

lysosome

Hsc70-4^HS1-25

macrochaeta | ectopic

Hsc70-4¹⁹⁵

melanotic mass

Hsc70-4⁰³⁵⁵⁰

mesonotum, with Scer\GAL4^pnr-MD237

Hsc70-4^GD11264

mesothoracic tergotrochanter muscle cell | progressive, with Scer\GAL4^Mhc.PK

Hsc70-4^RNAi.UAS.cUa

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

Hsc70-4^HMS00152

myofibril, with Scer\GAL4^Mef2.PR

Hsc70-4^GD11264

nucleus | spermatogenesis

Hsc70-4^Tag:FLAG

ommatidium, with Scer\GAL4^GMR.PF

Hsc70-4^K71S.UAS

ommatidium, with Scer\GAL4^GMR.PU

Hsc70-4^KK108223

photoreceptor neuron

Hsc70-4⁰³⁵⁵⁰

presumptive embryonic/larval tracheal system, with Scer\GAL4^btl.PS

Hsc70-4^K71S.UAS

sarcomere, with Scer\GAL4^Mef2.PR

Hsc70-4^GD11264

sense organ | ectopic

Hsc70-4¹⁹⁵

spermatocyte cyst, with Scer\GAL4^VP16.bam

Hsc70-4^KK108223

spermatozoon

Hsc70-4^Tag:FLAG

spermatozoon | absent, with Scer\GAL4^VP16.bam

Hsc70-4^KK108223

spermatozoon | decreased number

Hsc70-4^Tag:FLAG

testis, with Scer\GAL4^VP16.bam

Hsc70-4^KK108223

ventral denticle belt

Hsc70-4^54.1

wing margin

Hsc70-4¹⁹⁵

wing vein L3 & sensillum campaniformium | ectopic

Hsc70-4¹⁹⁵

Z disc, with Scer\GAL4^Mef2.PR

Hsc70-4^GD11264

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (21)

Hsap\HSPA8

12 of 14

Yes

Hsap\HSPA2

11 of 14

Yes

Hsap\HSPA1A

8 of 14

Yes

Hsap\HSPA1B

8 of 14

Yes

Hsap\HSPA1L

8 of 14

Yes

Hsap\HSPA6

8 of 14

Yes

Hsap\HSPA5

6 of 14

Hsap\HSPA9

4 of 14

Hsap\HSPA13

3 of 14

Yes

Hsap\HSPA4

2 of 14

Hsap\HSPA4L

2 of 14

Hsap\HSPA12A

2 of 14

Yes

Hsap\HSPA12B

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Hsap\DENND6A

1 of 14

Hsap\DENND6B

1 of 14

Hsap\HSPA7

1 of 14

Yes

Hsap\SLC9C2

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (20)

Rnor\Hspa8

12 of 14

Yes

Rnor\LOC680121

11 of 14

Yes

Rnor\Hsc70-ps1

10 of 14

Yes

Rnor\Hspa2

10 of 14

Yes

Rnor\Hspa1l

8 of 14

Yes

Rnor\Hspa1a

7 of 14

Yes

Rnor\Hspa1b

7 of 14

Yes

6 of 14

4 of 14

2 of 14

2 of 14

Yes

Rnor\Hspa12b

2 of 14

Yes

Rnor\Hspa13

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\LOC100363133

1 of 14

Yes

Mus musculus (laboratory mouse) (18)

Mmus\Hspa8

12 of 14

Yes

Mmus\Hspa2

10 of 14

Yes

Mmus\Hspa1a

8 of 14

Yes

Mmus\Hspa1b

8 of 14

Yes

Mmus\Hspa1l

8 of 14

Yes

Mmus\Hspa5

5 of 14

Mmus\Hspa9

4 of 14

Mmus\Hspa13

3 of 14

Yes

Mmus\Hspa4

2 of 14

Mmus\Hspa12a

2 of 14

Yes

Mmus\Hspa12b

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Mmus\Dennd6a

1 of 14

Mmus\Dennd6b

1 of 14

Mmus\Hspa4l

1 of 14

Xenopus tropicalis (Western clawed frog) (17)

Xtro\hspa1a

11 of 13

Yes

Xtro\hspa8

10 of 13

Yes

Xtro\hspa1l

7 of 13

Yes

Xtro\hsp70

4 of 13

Yes

Xtro\hspa1b

3 of 13

Xtro\hspa2

3 of 13

Yes

Xtro\hspa9

3 of 13

Xtro\tut7

3 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\hspa13

1 of 13

Yes

Xtro\hspa14

1 of 13

Xtro\hyou1

1 of 13

Xtro\prelid2

1 of 13

Yes

Xtro\XB5934250

1 of 13

Danio rerio (Zebrafish) (25)

Drer\hspa8b

13 of 14

Yes

Drer\hspa8

12 of 14

Yes

Drer\hsc70

11 of 14

Yes

Drer\hsp70.1

10 of 14

Yes

Drer\hsp70.3

10 of 14

Yes

Drer\hsp70l

10 of 14

Yes

Drer\hspa1b

9 of 14

Yes

Drer\hsp70.2

7 of 14

Yes

Drer\hspa5

5 of 14

Drer\hspa9

4 of 14

Drer\hspa13

3 of 14

Yes

Drer\hspa4a

2 of 14

Drer\hspa4b

2 of 14

Drer\hspa12a.3

2 of 14

Yes

Drer\hspa12a

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\hspa12a.2

1 of 14

Yes

Drer\hspa12b

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\hsp-1

12 of 14

Yes

Cele\F44E5.4

6 of 14

Yes

Cele\F44E5.5

6 of 14

Yes

Cele\hsp-3

6 of 14

Cele\hsp-4

6 of 14

Cele\hsp-70

6 of 14

Yes

Cele\hsp-6

4 of 14

Cele\stc-1

3 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (12)

Agam\AgaP_AGAP002076

12 of 12

Yes

7 of 12

5 of 12

5 of 12

4 of 12

4 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Yes

Agam\AgaP_AGAP004946

1 of 12

Arabidopsis thaliana (thale-cress) (21)

Atha\HSC70-1

13 of 13

Yes

Atha\AT3G09440

12 of 13

Atha\ERD2

12 of 13

Yes

Atha\Hsp70-2

12 of 13

Yes

Atha\HSP70

11 of 13

Yes

Atha\Hsp70b

11 of 13

Yes

6 of 13

6 of 13

6 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (14)

Scer\SSA1

11 of 13

Yes

Scer\SSA2

11 of 13

Yes

Scer\SSA3

11 of 13

Yes

Scer\SSA4

11 of 13

Yes

Scer\KAR2

5 of 13

Scer\SSB1

5 of 13

Yes

Scer\SSB2

5 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (8)

Spom\ssa1

11 of 12

Yes

Spom\ssa2

11 of 12

Yes

Spom\bip1

5 of 12

Spom\sks2

4 of 12

Yes

3 of 12

3 of 12

2 of 12

2 of 12

Yes

Escherichia coli (enterobacterium) (4)

Ecol\dnaK

3 of 11

Yes

Ecol\hscA

2 of 11

Ecol\hscC

2 of 11

Ecol\yegD

2 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Hsc70-4. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (15)

11 of 13

8 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

6 of 13

6 of 13

4 of 13

3 of 13

3 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Hsc70-4^+t14

model of ataxia telangiectasia

CEA with tefu^Δ356

(Oikemus et al., 2004, Song et al., 2004)

CEA with tefu^Δ11

(Song et al., 2004)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 10 )

Allele

Disease

Interaction

References

Hsc70-4^Δ356

ameliorates neuronal ceroid lipofuscinosis 4

modeled by Hsap\DNAJC5^L116Δ.UAS

(Imler et al., 2019)

modeled by Hsap\DNAJC5^L115R.UAS

(Imler et al., 2019)

Hsc70-4^K71S.UAS

exacerbates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Huen and Chan, 2005)

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Ghosh and Feany, 2004)

modeled by Zzzz\CGG^90.UAS.EGFP

(Jin et al., 2003)

exacerbates Parkinson's disease

modeled by Hsap\SNCA^UAS.cFa

(Auluck et al., 2002)

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82.UAS

(Ghosh and Feany, 2004)

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Ghosh and Feany, 2004)

exacerbates Kennedy's disease

modeled by Hsap\AR^{Q112.UAS.Tag:HA}, Prosβ6^1.UAS

(Chan et al., 2002)

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Warrick et al., 1999)

ameliorates Huntington's disease

modeled by Hsap\HTT^Q48.ex1p.UAS

(Iijima-Ando et al., 2005)

modeled by Hsap\HTT^Q48.ex1p.UAS, CrebB^+tIa

(Iijima-Ando et al., 2005)

modeled by Hsap\HTT^{N548.Q128.UAS}

(Prakash et al., 2022)

exacerbates erythrokeratodermia variabilis

modeled by Hsap\GJB3^{F137L.UAS.Tag:MYC}

(Tang et al., 2015)

Hsc70-4^L3929

exacerbates neuronal ceroid lipofuscinosis

modeled by cathD¹

(Kuronen et al., 2009)

Hsc70-4^UAS.cEa

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Gunawardena et al., 2003)

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Gunawardena et al., 2003)

modeled by Hsap\ATXN3^{Q77.UAS.Tag:HA,Tag:NES(HIV-1-Rev)}

(Gunawardena et al., 2003)

modeled by Zzzz\CGG^90.UAS.EGFP

(Jin et al., 2003)

ameliorates erythrokeratodermia variabilis

modeled by Hsap\GJB3^{F137L.UAS.Tag:MYC}

(Tang et al., 2015)

ameliorates fragile X-associated tremor/ataxia syndrome

modeled by Zzzz\CGG^90.UAS.EGFP

(Oh et al., 2015)

ameliorates obesity

modeled by HDAC6^KO

(Yan et al., 2017)

modeled by Lsd-2^UAS.EYFP

(Yan et al., 2017)

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^UAS.cEa

(Berson et al., 2017)

exacerbates Huntington's disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Rai and Tapadia, 2022)

modeled by Hsap\HTT^Q93.ex1.UAS

(Rai and Tapadia, 2022)

Hsc70-4^GD11264

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Voßfeldt et al., 2012)

Hsc70-4^GD16584

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Voßfeldt et al., 2012)

Hsc70-4^JF03136

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Johnson et al., 2020, Tsou et al., 2015)

modeled by Hsap\ATXN3^fl.SCA3.UAS

(Johnson et al., 2020)

modeled by Hsap\ATXN3^{80Q.UAS.Tag:HA}

(Johnson et al., 2020)

ameliorates Huntington's disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Rai and Tapadia, 2022)

modeled by Hsap\HTT^Q93.ex1.UAS

(Rai and Tapadia, 2022)

Hsc70-4^GLV21049

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^fl.SCA3.UAS

(Johnson et al., 2020)

modeled by Hsap\ATXN3^{80Q.UAS.Tag:HA}

(Johnson et al., 2020)

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Johnson et al., 2020)

Hsc70-4^HMS00152

ameliorates neuronal ceroid lipofuscinosis 4

modeled by Hsap\DNAJC5^L116Δ.UAS

(Imler et al., 2019)

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^fl.SCA3.UAS

(Johnson et al., 2020)

modeled by Hsap\ATXN3^{80Q.UAS.Tag:HA}

(Johnson et al., 2020)

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Johnson et al., 2020)

ameliorates Huntington's disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Rai and Tapadia, 2022)

modeled by Hsap\HTT^Q93.ex1.UAS

(Rai and Tapadia, 2022)

Hsc70-4^{wt.UAS.Tag:HA}

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^G298S.UAS

(Coyne et al., 2017)

modeled by Hsap\TARDBP^UAS.Tag:MYC

(Coyne et al., 2017)

modeled by Hsap\TARDBP^{Q331K.UAS.cLa}

(Coyne et al., 2017)

modeled by Hsap\TARDBP^{M337V.UAS.cLa}

(Coyne et al., 2017)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\GGGGCC^36.UAS

(Coyne et al., 2017)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

HSPA8; heat shock protein family A (Hsp70) member 8

12 of 14

600816

HSPA2; heat shock protein family A (Hsp70) member 2

11 of 14

140560

HSPA1A; heat shock protein family A (Hsp70) member 1A

8 of 14

140550

HSPA1B; heat shock protein family A (Hsp70) member 1B

8 of 14

603012

HSPA1L; heat shock protein family A (Hsp70) member 1 like

8 of 14

140559

HSPA6; heat shock protein family A (Hsp70) member 6

8 of 14

140555

HSPA5; heat shock protein family A (Hsp70) member 5

6 of 14

138120

HSPA9; heat shock protein family A (Hsp70) member 9

4 of 14

600548

autosomal dominant sideroblastic anemia 4

HSPA13; heat shock protein family A (Hsp70) member 13

3 of 14

601100

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Hsc70-4 - AGO1

anti tag coimmunoprecipitation, Identification by mass spectrometry, pull down, western blot

(Iwasaki et al., 2010)

Hsc70-4 - AGO2

anti tag coimmunoprecipitation, Identification by mass spectrometry, pull down, western blot

(Iwasaki et al., 2010)

Hsc70-4 - AGO3

anti bait coimmunoprecipitation, western blot

(Cappucci et al., 2019)

Hsc70-4 - CG17343

experimental knowledge based

(Guruharsha et al., 2011)

Hsc70-4 - CG7945

tandem affinity purification, multidimensional protein identification technology

(Dyer et al., 2017)

Hsc70-4 - DnaJ-1

tandem affinity purification, multidimensional protein identification technology

(Dyer et al., 2017)

Hsc70-4 - EcR

anti bait coimmunoprecipitation, Identification by mass spectrometry

(Xie et al., 2015)

Hsc70-4 - Elp3

pull down, Identification by mass spectrometry, molecular sieving, molecular weight estimation by staining

(Planelles-Herrero et al., 2022)

Hsc70-4 - HDAC6

enzymatic study, autoradiography, anti tag coimmunoprecipitation, anti tag western blot

(Yan et al., 2017)

Hsc70-4 - Hecw

pull down, Identification by mass spectrometry

(Fajner et al., 2021)

Hsc70-4 - Hsap\TARDBP

anti tag coimmunoprecipitation, western blot, quantitative reverse transcription pcr

(Coyne et al., 2017)

Hsc70-4 - Lsd-2

anti tag coimmunoprecipitation, western blot

(Yan et al., 2017)

Hsc70-4 - Mlf

tandem affinity purification, multidimensional protein identification technology

(Dyer et al., 2017)

Hsc70-4 - Sgt

pull down, western blot, enzymatic study, luminiscence technology, anti tag coimmunoprecipitation

(Uytterhoeven et al., 2015)

Hsc70-4 - Stip1

affinity chromatography technology, molecular weight estimation by staining, Identification by mass spectrometry

(Iwasaki et al., 2015)

Hsc70-4 - Tcs3

experimental knowledge based

(Guruharsha et al., 2011)

Hsc70-4 - Zw10

anti bait coimmunoprecipitation, peptide massfingerprinting

(Williams et al., 2003)

Hsc70-4 - Zwilch

anti bait coimmunoprecipitation, peptide massfingerprinting

(Williams et al., 2003)

Hsc70-4 - brm

pull down, ion exchange chromatography, molecular sieving, edman degradation

(Papoulas et al., 1998)

Hsc70-4 - mrj

anti bait coimmunoprecipitation, anti tag western blot

(Desai et al., 2024)

Hsc70-4 - orb2

anti bait coimmunoprecipitation, anti tag western blot

(Desai et al., 2024)

Hsc70-4 - osk

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Tanaka et al., 2021)

Hsc70-4 - ph-p

anti tag coimmunoprecipitation, peptide massfingerprinting

(Saurin et al., 2001)

Hsc70-4 - spag

pull down, autoradiography, anti tag coimmunoprecipitation, peptide massfingerprinting

(Benbahouche et al., 2014)

Hsc70-4 - stumps

two hybrid

(Battersby et al., 2003)

Hsc70-4 - stv

anti tag coimmunoprecipitation, western blot, two hybrid

(Brooks et al., 2020, Arndt et al., 2010)

Hsc70-4 - usp

anti bait coimmunoprecipitation, Identification by mass spectrometry

(Xie et al., 2015)

RNA-protein

Physical Interaction

Assay

References

Hsc70-4 - Hsap\TARDBP

anti tag coimmunoprecipitation, western blot, quantitative reverse transcription pcr

(Coyne et al., 2017)

Hsc70-4 - bol

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, pull down, Identification by mass spectrometry

(Gao et al., 2020)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Hsc70-4

enhanceable

aux

(Hagedorn et al., 2006)

Hsc70-4

enhanceable

Csp

(Bronk et al., 2001)

Hsc70-4

suppressible

(Hing et al., 1999)

Hsc70-4

enhanceable

NdufA3

(Pueyo et al., 2023)

Hsc70-4

enhanceable

Rme-8

(Chang et al., 2004)

Hsc70-4

enhanceable

Tsc1

(Hennig et al., 2006)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Asx

enhanceable

Hsc70-4

(Mollaaghababa et al., 2001)

suppressible

(Gao et al., 2020)

enhanceable

(Kuronen et al., 2009)

CrebB

suppressible

Hsc70-4

(Iijima-Ando et al., 2005)

Csp

enhanceable

Hsc70-4

(Bronk et al., 2001, Wenniger et al., 1998)

CycE

suppressible

Hsc70-4

(Brumby et al., 2002)

enhanceable

Hsc70-4

(Hing et al., 1999)

suppressible

(Yan et al., 2017)

suppressible

(Yan et al., 2017)

enhanceable

(Hennig et al., 2006)

suppressible

Hsc70-4

(Hing et al., 1999)

enhanceable

Hsc70-4

(Hing et al., 1999)

NdufA3

enhanceable

Hsc70-4

(Pueyo et al., 2023)

NdufA3

suppressible

Hsc70-4

(Pueyo et al., 2023)

enhanceable

Hsc70-4

(Wang and Brock, 2003, Mollaaghababa et al., 2001)

suppressible

Hsc70-4

(Mollaaghababa et al., 2001)

Pcl

enhanceable

Hsc70-4

(Mollaaghababa et al., 2001)

ph-d

enhanceable

Hsc70-4

(Wang and Brock, 2003)

ph-p

enhanceable

Hsc70-4

(Wang and Brock, 2003, Mollaaghababa et al., 2001)

ph-p

suppressible

Hsc70-4

(Mollaaghababa et al., 2001)

shi

enhanceable

Hsc70-4

(Chang et al., 2002)

Snr1

enhanceable

Hsc70-4

(Marenda et al., 2004)

Tpi

suppressible

Hsc70-4

(Hrizo and Palladino, 2010)

Tpi

enhanceable

Hsc70-4

(Hrizo and Palladino, 2010)

trx

enhanceable

Hsc70-4

(Mollaaghababa et al., 2001)

trx

suppressible

Hsc70-4

(Mollaaghababa et al., 2001)

Tsc1

enhanceable

Hsc70-4

(Hennig et al., 2006)

External Data

Subunit Structure (UniProtKB)

Associates with the elongator complex; this interaction is required for some functions of the elongator complex but is not required for regulation of microtubule dynamics.

(UniProt, P11147 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0266599

MIST (genetic) - An integrated Molecular Interaction Database

41840

MIST (protein-protein) - An integrated Molecular Interaction Database

41840

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-56

Cytogenetic map

88E4-88E4

Sequence location

3R:15,242,687..15,246,614 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

88E4-88E4

Limits computationally determined from genome sequence between P{EP}CG33967^EP666 and P{lacW}Hsc70-4^L3929

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

88E8-88E9

(determined by in situ hybridisation)

(Spradling et al., 1999, Perrimon et al., 1996)

88E3-88E4

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-56

(FlyBase, 2016)

3-55.8

(Comeron et al., 2012)

3-56.5

(Hing et al., 1999)

Left of (cM)

(Mollaaghababa et al., 2001)

(Mollaaghababa et al., 2001, Hing et al., 1999)

Right of (cM)

(Mollaaghababa et al., 2001)

(Hing et al., 1999)

Notes

Stocks and Reagents

Stocks (28)

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1530)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

LD25749

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0266599

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The Hsc70-4 protein has two separable functions: chaperone activity and membrane deformation activity. The membrane deforming activity of Hsc70-4 requires oligomerisation and membrane association. The factors that promote the chaperone activity of Hsc70-4 (ATP and the presence of the Sgt cochaperone) simultaneously inhibit its membrane deforming activity. The membrane deforming activity of Hsc70-4 is necessary and sufficient for endosomal microautophagy at synapses, which promotes the turnover of a number of synaptic proteins. Both the chaperone activity and membrane deformation activity of Hsc70-4 are needed to maintain normal levels of synaptic transmission.

(Uytterhoeven et al., 2015)

Hsc70-4 mutation disrupts clathrin function and affects early steps of the endocytic pathway.

(Chang et al., 2003)

Interacts gentically with: Fmr1

(Jin et al., 2003)

sev-mediated internalization of boss protein is blocked in mutant Hsc70-4 eye disc epithelial cells.

(Chang et al., 2002)

Hsc70-4 is critical for neurotransmitter exocytosis.

(Bronk et al., 2001)

Area matching Drosophila EST AI294469.

(Davies, 2001.3.15)

The gene products of Hsp83 and the Hsc70 genes are present in a chaperone complex required for activation of DNA binding by the EcR/usp complex. The gene products of Hsp83 and the Hsc70 genes are also required in vivo for EcR activity and EcR is the primary target of the chaperone complex.

(Arbeitman and Hogness, 2000)

Hsc70-4 is thought to be involved in subcellular trafficking and may affect the number of functional N receptors on the cell surface.

(Hing et al., 1999)

Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell dysregulation.

(Braun et al., 1997)

Isolated during a screen for mutations that disrupt Bolwig's organ or Bolwig's nerve development.

(Schmucker et al., 1997)

The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(w^hs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.

(Perrimon et al., 1996)

Members of the hsc70 gene family (heat shock cognate genes) that reside within the same intracellular compartment in different organisms share greater amino acid identity than hsc70 proteins from the same organism but different organelles. This pattern of conservation indicates specialisation of hsc70 function.

(Rubin et al., 1993)

First recognised by sequence similarity to Hsp70. Transcription is developmentally regulated, but is temperature independent.

(Lindsley and Zimm, 1992)

transcript abundance embryo: ++++ transcript abundance larva: ++++ transcript abundance adult: ++++

Relationship to Other Genes

Source for database merge of

Source for merge of: scattered Hsc70-4

(Wenniger et al., 1998)

Source for merge of: Hsc70-4 l(3)L3929

(Beaton, 1999.12.12)

Source for merge of: Hsc70-4 anon-WO0118547.237

(FlyBase, 1996-)

Additional comments

The Hsc70-4 gene may have been derived from the Hsc70-3 gene by retroposition.

(Pan and Zhang, 2009)

The Hsc70-4 gene may have been derived from the Hsc70-1 gene by retroposition.

(Bai et al., 2007)

Source for merge of Hsc70-4 anon-WO0118547.237 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Hsc70-4 CG4264

(Zheng, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (55)

Reported As

Symbol Synonym

BAP74

(Bouazoune and Brehm, 2006, Eberharter and Becker, 2004, Kingston and Narlikar, 1999, Wade and Wolffe, 1999, Papoulas et al., 1998)

Bap74

C7LA75

(Ebanks et al., 2021)

E(csp)1545

(Wenniger et al., 1998)

E(nd)195

(Doughty, 2000.5.9)

HSC-70

(Richmond and Broadie, 2002)

HSC4

(Li et al., 2012, Fernandez-Funez et al., 2009, Chen et al., 2005, Saurin et al., 2001, Elefant and Palter, 1997, Elefant and Palter, 1995, Rubin et al., 1993)

HSC70

(Folk et al., 2006, Gunawardena et al., 2003, Singh and Lakhotia, 1995)

HSC70-4

(Lovejoy et al., 2021, Naz et al., 2020, Kumada et al., 2019, Hsiao et al., 2014, Kuo et al., 2013, Singh et al., 2010, Wong et al., 2008)

HSP70

(Oh et al., 2015)

HSP70cB2

(Kaneko, 2022)

HSP7D

(Beghelli et al., 2024)

Hsc-4

(Nikolaidis and Nei, 2004)

Hsc-70-4

(Chang et al., 2008)

Hsc4p

(Elefant and Palter, 1999)

Hsc70

(Himalian et al., 2022, Sabath et al., 2013, Fredriksson et al., 2012, Arndt et al., 2010, Wong et al., 2008, Korolchuk et al., 2007, Hagedorn et al., 2006, Wellington and Zinsmaier, 2005, Gunawardena et al., 2003, Tatar et al., 2003, Chan et al., 2002, Zoghbi and Botas, 2002, Begun and Whitley, 2000, Yue et al., 1999, Wilke et al., 1994)

Hsc70-4

(Collins et al., 2024, Deo et al., 2024, Desai et al., 2024, Malik et al., 2024, Meyer et al., 2024, Sood et al., 2024, Vesala et al., 2024, Wang et al., 2024, Everman et al., 2023, Koehler and Huber, 2023, Pueyo et al., 2023, Yheskel et al., 2023, Huang et al., 2022, Kaneko, 2022, Känel et al., 2022, Perlegos et al., 2022, Planelles-Herrero et al., 2022, Prakash et al., 2022, Rai and Tapadia, 2022, van de Leemput et al., 2022, Yang and Zhang, 2022, Azuma et al., 2021, Evans et al., 2021, Girard et al., 2021, Mesquita et al., 2021, Pang et al., 2021, Parkhitko et al., 2021, Ryan et al., 2021, Silva et al., 2021, Tanaka et al., 2021, Wang et al., 2021, Brooks et al., 2020, Gohel et al., 2020, Johnson et al., 2020, Khan et al., 2020, Tsuboyama et al., 2020, Vásquez-Procopio et al., 2020, Sørensen et al., 2019, Zhou et al., 2019, Kucherenko and Shcherbata, 2018, Stephano et al., 2018, Thomas et al., 2018, Tsuboyama et al., 2018, Coyne et al., 2017, Dyer et al., 2017, Galluzzi et al., 2017, Lin et al., 2017, Miller et al., 2017, Transgenic RNAi Project members, 2017-, Clandinin and Owens, 2016-, Mukherjee et al., 2016, Tan et al., 2016, Yu et al., 2016, Dent et al., 2015, Horan et al., 2015, Iwasaki et al., 2015, Jia et al., 2015, Štětina et al., 2015, Tang et al., 2015, Xie et al., 2015, Ashwal-Fluss et al., 2014, Benbahouche et al., 2014, dos Santos, 2014, Kim and Choe, 2014, Lee et al., 2014, Mukherjee et al., 2014, Radermacher et al., 2014, Slawson et al., 2014, Colinet et al., 2013, Kockmann et al., 2013, Kwon et al., 2013, O'Sullivan et al., 2013, Telonis-Scott et al., 2013, Yin et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Franco et al., 2011, Friedman et al., 2011, Rees et al., 2011, Toku et al., 2011, Wertheim et al., 2011, Iwasaki et al., 2010, Kallappagoudar et al., 2010, Kim et al., 2010, Müller et al., 2010, Wasbrough et al., 2010, Zhang et al., 2010, Ahlander and Bosco, 2009, Gruenewald et al., 2009, Herold et al., 2009, Pisa et al., 2009, Takemori and Yamamoto, 2009, Worringer et al., 2009, Allen and Spradling, 2008, Bettencourt et al., 2008, Puri et al., 2008, Bai et al., 2007, Buszczak et al., 2007, Derré et al., 2007, Nekrasov et al., 2007, Stuart et al., 2007, Bandyopadhyay et al., 2006, Beller et al., 2006, Beller et al., 2006, Cermelli et al., 2006, Chartier et al., 2006, Dorner et al., 2006, Hagedorn et al., 2006, Hennig et al., 2006, Molnar et al., 2006, Neal et al., 2006, Walser et al., 2006, Rehwinkel et al., 2004, Stanyon et al., 2004)

Hsc70.4

(François-Moutal et al., 2022, Kumar and Tiwari, 2018)

Hsp-c4

(Fisher et al., 2008)

Hsp70

(Tsuboyama et al., 2018, Qurashi et al., 2011, Hrizo and Palladino, 2010)

Q3KN45_DROME

(Fukuyama et al., 2012)

anon-WO0118547.237

bs17d06.y1

(Andrews et al., 2000)

chr3R:11070155..11070315

(Will et al., 2013)

dHSP70

(Tang et al., 2015)

dHsp70

(Tang et al., 2015)

dhsc70

(Rabindran et al., 1994)

hsc4

(Kim et al., 2015, Cowan et al., 2011, Dickman and Davis, 2009, Yang et al., 2006, Shulman and Feany, 2003, Bronk et al., 2001, Mollaaghababa et al., 2001, van Steensel et al., 2001, Arbeitman and Hogness, 2000, Hing et al., 1999, Warrick et al., 1999, Shopland and Lis, 1996, Noll et al., 1993)

hsc70

(González-Méndez et al., 2020, Kang et al., 2002, Mehta et al., 1995, Mehta et al., 1991, Ornelles and Penman, 1990, Perkins et al., 1990)

hsc70-4

(Jacomin et al., 2021, Xie et al., 2021, Imler et al., 2019, Uytterhoeven et al., 2015, Zhang et al., 2010, Kuronen et al., 2009, Zhou et al., 2008, Behr et al., 2007, Bi et al., 2004, Cherry and Perrimon, 2004, Clyne et al., 2003)

hsp70

(Iijima-Ando et al., 2005)

i190

(Battersby et al., 2003)

l(3)03550

l(3)L3929

(BDGP Project Members, 1994-1999)

l(3)j7A4

mtHsp 70

(Baqri et al., 2014)

scd

(Schmucker et al., 1997)

Name Synonyms

70 kDa heat shock protein, cytosolic B 2

(Kaneko, 2022)

Bap74

Heat shock 70 kDa protein cognate 4

(Urban et al., 2017)

Heat shock 70-kDa protein cognate 4

(Pflieger et al., 2008)

Heat shock cognate 4

(Wang and Brock, 2003)

Heat shock protein 70

(Robinson et al., 2010)

Heat shock protein 70 cognate 4

Heat shock protein cognate 4

(Ebanks et al., 2021, McEachern et al., 2014, Jaspers et al., 2012, Cammarato et al., 2011, Pisa et al., 2009)

Hsp cognate 4

(Takemori and Yamamoto, 2009)

heat shock cognate 4

(Ghosh and Feany, 2004)

heat shock cognate 70

(Wong et al., 2008)

heat shock cognate protein 4

(Cermelli et al., 2006)

heat shock cognate protein 70-4

(Behr et al., 2007)

heat shock protein cognate 4

(Breloy et al., 2016)

heat-shock protein 70

(Perez and Hastings, 2004)

hsp cognate 4

(Fakhouri et al., 2006)

scattered

(Wenniger et al., 1998, Schmucker et al., 1997)

Secondary FlyBase IDs

FBgn0001219
FBgn0010802
FBgn0011400
FBgn0024549
FBgn0062154
FBgn0066308

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 82 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41840

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P11147

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P11147

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

C7LA75

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P11147

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266599

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41840/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266599

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266599

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4264

MARRVEL_MODEL - MARRVEL (model organism gene)

41840

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0266599

DroID - A comprehensive database of gene and protein interactions.

FBgn0266599

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001219

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0266599

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4264

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0266599

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031639

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

41840

MIST (protein-protein) - An integrated Molecular Interaction Database

41840

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (347)

The following external sites may use different assemblies or annotations than FlyBase.

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