FlyBase Gene Report: Dmel\Mcr

General Information

Symbol

Dmel\Mcr

Species

D. melanogaster

Name

Macroglobulin complement-related

Annotation Symbol

CG7586

Feature Type

protein_coding_gene

FlyBase ID

FBgn0267488

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

Macroglobulin complement-related (Mcr) encodes a transmembrane protein of the conserved thioester-containing protein (TEP) superfamily including macroglobulins and complement proteins. It is a core component of septate junctions, which provide an essential paracellular barrier to epithelia. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Also Known As

Tep6, E(br)155, dTEPVI, TepVI

Key Links

Genomic Location

Cytogenetic map

28E1-28E3

Sequence location

2L:8,074,117..8,082,982 [-]

Recombination map

2-30

RefSeq locus

NT_033779 REGION:8074117..8082982

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables structural molecule activity

inferred from mutant phenotype

(Hall et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables endopeptidase inhibitor activity

inferred from electronic annotation with InterPro:IPR001599, InterPro:IPR002890

(InterPro Project Members, 2004-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in defense response to fungus

inferred from mutant phenotype

(Stroschein-Stevenson et al., 2006)

involved_in dorsal closure

inferred from mutant phenotype

(Ward et al., 2003)

involved_in head involution

inferred from mutant phenotype

(Ward et al., 2003)

involved_in phagocytosis, recognition

inferred from direct assay

(Stroschein-Stevenson et al., 2006)

inferred from mutant phenotype

(Stroschein-Stevenson et al., 2006)

involved_in regulation of tube length, open tracheal system

inferred from mutant phenotype

(Bätz et al., 2014, Hall et al., 2014)

involved_in septate junction assembly

inferred from mutant phenotype

(Bätz et al., 2014, Hall et al., 2014)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (9 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

located_in apical plasma membrane

inferred from direct assay

(Hall et al., 2014)

located_in apicolateral plasma membrane

inferred from direct assay

(Hall et al., 2014)

located_in basolateral plasma membrane

inferred from direct assay

(Hall et al., 2014)

located_in extracellular space

inferred from direct assay

(Stroschein-Stevenson et al., 2006)

located_in lateral plasma membrane

inferred from direct assay

(Bätz et al., 2014)

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

located_in septate junction

inferred from direct assay

(Bätz et al., 2014, Hall et al., 2014)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in extracellular region

inferred from electronic annotation with InterPro:IPR009048, InterPro:IPR036595

(InterPro Project Members, 2004-)

located_in extracellular space

inferred from electronic annotation with InterPro:IPR011626

(InterPro Project Members, 2004-)

located_in membrane

inferred from sequence model

(Bätz et al., 2014)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Mcr for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VLT3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Mcr-RA

FBtr0079543

NM_079949

6296

1760

Additional Transcript Data and Comments

Reported size (kB)

8 (northern blot)

(Vallejo and Leon, 1989)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Mcr-PA

FBpp0079165

202.8

1760

6.42

Q9VLT3

NP_524688

AAF52601

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VLT3

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mcr using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mcr_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.35

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval peripheral nervous system

(Crowley, 1997.7.14)

spermatogenesis & adult stage

testis

(Crowley, 1997.7.14)

spermatocyte

(Crowley, 1997.7.14)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10 -- 17

embryonic hindgut

(Bätz et al., 2014)

embryonic foregut

(Bätz et al., 2014)

embryonic stage 11 -- 17

embryonic/larval salivary gland

(Bätz et al., 2014)

embryonic/larval tracheal section

(Bätz et al., 2014)

embryonic epidermis

(Bätz et al., 2014)

larval stage

embryonic/larval plasmatocyte

(Bou Aoun et al., 2011)

imaginal disc

(Bou Aoun et al., 2011)

adult stage

epithelium of crop

(Bou Aoun et al., 2011)

adult proventriculus

(Bou Aoun et al., 2011)

epidermis of abdomen | ventral

(Bou Aoun et al., 2011)

mesothoracic phragma

(Bou Aoun et al., 2011)

Additional Descriptive Data

Mcr expression is detected in plasmatocytes and in all imaginal discs in larvae. In adults it is observed in the proventriculus, in the crop epithelium and in the epidermis underlying the cuticle in the abodomen but only on the ventral side. Expression is also detected in the mesothoracic phragma, the structure found at the border between thorax and abdomen and to which flight muscles are attached posteriorly.

(Bou Aoun et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 17

embryonic epidermis

(Bätz et al., 2014)

embryonic stage 16

embryonic/larval salivary gland

• apical plasma membrane

(Bätz et al., 2014)

embryonic/larval salivary gland

• apicolateral plasma membrane

(Bätz et al., 2014)

embryonic hindgut

(Bätz et al., 2014)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in apical plasma membrane

inferred from direct assay

(Hall et al., 2014)

located_in apicolateral plasma membrane

inferred from direct assay

(Hall et al., 2014)

located_in basolateral plasma membrane

inferred from direct assay

(Hall et al., 2014)

located_in extracellular space

inferred from direct assay

(Stroschein-Stevenson et al., 2006)

located_in lateral plasma membrane

inferred from direct assay

(Bätz et al., 2014)

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

located_in septate junction

inferred from direct assay

(Bätz et al., 2014, Hall et al., 2014)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mcr in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mcr

View exonic expression by tissue for Dmel\Mcr

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44071/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267488

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020240

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267488

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7586

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mcr

Transgenic constructs containing regulatory region of Mcr

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2L)Exel7034

(Bätz et al., 2014, Ryder, 2004.4.26)

Df(2L)Trf-C6R31

(Bätz et al., 2014, Crowley et al., 1993)

Gene not disrupted in

Df(2L)TE29Aa-11

(Bätz et al., 2014)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Mcr^Pex3

lethal (with Mcr^D13)

Mcr^EY07421

lethal (with Mcr^EY07421)

lethal (with Mcr^G249)

Mcr^EY07421

lethal (with Mcr^H25)

Mcr^EY07421

lethal (with Mcr^K101)

Mcr^EY07421

lethal (with Mcr^K103)

Mcr^EY07421

lethal - all die during embryonic stage

lethal - all die during embryonic stage | recessive

Mcr¹

lethal - all die during larval stage

Mcr^H25

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Mcr^GD988

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Mcr^GD988

viable, with Scer\GAL4^Mef2.PR

Mcr^GD988

Other Phenotypes

Allele

majority die during embryonic stage

Mcr^Pex3

some die during embryonic stage | recessive

Mcr¹

some die during pupal stage, with Scer\GAL4^pnr-MD237

Mcr^GD988

visible, with Scer\GAL4^pnr-MD237

Mcr^GD988

visible | semidominant

Mcr¹

Phenotype manifest in

Allele

chaeta, with Scer\GAL4^pnr-MD237

Mcr^GD988

embryonic/first instar larval cuticle | anterior

Mcr¹

embryonic/first instar larval cuticle | dorsal

Mcr¹

embryonic/larval hindgut

embryonic/larval salivary gland

embryonic/larval tracheal dorsal trunk

Mcr^K103

larval tracheal system

larval tracheal system, with Scer\GAL4^btl.PS

Mcr^KK104742

larval tracheal system (with Mcr^D13)

Mcr^EY07421

larval tracheal system (with Mcr^EY07421)

larval tracheal system (with Mcr^G249)

Mcr^EY07421

larval tracheal system (with Mcr^H25)

Mcr^EY07421

larval tracheal system (with Mcr^K101)

Mcr^EY07421

larval tracheal system (with Mcr^K103)

Mcr^EY07421

larval tracheal system | embryonic stage, with Scer\GAL4^btl.PS

Mcr^KK104742

leg

Mcr¹

septate junction

septate junction, with Dcr-2^UAS.cDa, Scer\GAL4^ap-md544

Mcr^KK104742

trichogen cell, with Scer\GAL4^pnr-MD237

Mcr^GD988

ventral denticle belt

ventral denticle belt, with Scer\GAL4^da.PU

Mcr^KK104742

wing disc, with Scer\GAL4^ap-md544

Mcr^KK104742

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (15)

Hsap\CD109

5 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

1 of 14

Yes

Hsap\LOC408186

1 of 14

Hsap\LOC110384692

1 of 14

Yes

Hsap\OVOS2

1 of 14

Hsap\WASHC1

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (12)

Rnor\A2m

4 of 14

Yes

Rnor\C4a

4 of 14

Yes

Rnor\C4b

4 of 14

Yes

Rnor\Cd109

4 of 14

Yes

Rnor\LOC297568

4 of 14

Yes

Rnor\Mug1

4 of 14

Yes

Rnor\Mug2

4 of 14

Yes

Rnor\Pzp

4 of 14

Yes

3 of 14

3 of 14

1 of 14

1 of 14

Yes

Mus musculus (laboratory mouse) (12)

Mmus\Cd109

5 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (39)

Xtro\LOC100170611

4 of 13

Yes

Xtro\c4a

3 of 13

Yes

Xtro\acap2

2 of 13

Xtro\c3

2 of 13

Yes

Xtro\c5

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Xtro\LOC100492134

2 of 13

Yes

Xtro\ovos2

2 of 13

Xtro\ptcd2

2 of 13

Yes

Xtro\a2ml1

1 of 13

Xtro\a2m

1 of 13

Xtro\c3p1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100488897

1 of 13

Yes

Xtro\LOC100489060

1 of 13

Yes

Xtro\LOC100494685

1 of 13

Yes

Xtro\LOC100495827

1 of 13

Yes

Xtro\LOC100497762

1 of 13

Yes

Xtro\LOC101730404

1 of 13

Yes

Xtro\LOC101730963

1 of 13

Yes

Xtro\LOC101731171

1 of 13

Yes

Xtro\LOC101732535

1 of 13

Yes

Xtro\LOC101734901

1 of 13

Yes

Xtro\LOC105945599

1 of 13

Yes

Xtro\LOC108644460

1 of 13

Yes

Xtro\LOC108645185

1 of 13

Yes

Xtro\LOC108646250

1 of 13

Yes

Xtro\LOC108647609

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (31)

Drer\a2ml

4 of 14

Yes

Drer\c4b

4 of 14

Yes

Drer\c4

4 of 14

Yes

Drer\c5

4 of 14

Yes

Drer\cd109

4 of 14

Yes

Drer\si:ch211-212c13.10

4 of 14

Yes

Drer\si:dkey-46g23.2

4 of 14

Yes

Drer\si:dkey-46g23.5

4 of 14

Yes

Drer\zgc:165453

4 of 14

Yes

Drer\zgc:165518

4 of 14

Yes

Drer\zgc:171445

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Drer\si:dkey-105h12.2

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Drer\LOC407643

1 of 14

Yes

Drer\LOC569025

1 of 14

Yes

Drer\LOC100006895

1 of 14

Yes

Drer\LOC101886227

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\tep-1

3 of 14

Yes

Cele\F13D2.1

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (15)

Agam\TEP13

12 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (1)

Ecol\yfhM

4 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Mcr. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (5)

5 of 13

5 of 13

5 of 13

5 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CD109; CD109 molecule

5 of 14

608859

C4A; complement C4A (Chido/Rodgers blood group)

4 of 14

120810

complement component 4a deficiency

C4B; complement C4B (Chido/Rodgers blood group)

4 of 14

120820

COMPLEMENT COMPONENT 4B DEFICIENCY; C4BD

complement component 4b deficiency

C5; complement C5

4 of 14

120900

complement component 5 deficiency

A2M; alpha-2-macroglobulin

3 of 14

103950

A2ML1; alpha-2-macroglobulin like 1

3 of 14

610627

OTITIS MEDIA, SUSCEPTIBILITY TO; OMS

C3; complement C3

3 of 14

120700

C4B_2; complement component 4B (Chido/Rodgers blood group), copy 2

3 of 14

CPAMD8; C3 and PZP like alpha-2-macroglobulin domain containing 8

3 of 14

608841

ANTERIOR SEGMENT DYSGENESIS 8; ASGD8

anterior segment dysgenesis 8

PZP; PZP alpha-2-macroglobulin like

3 of 14

176420

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Mcr - LRP1

experimental knowledge based

(Guruharsha et al., 2011)

Mcr - egg

two hybrid

(Stabell et al., 2006)

RNA-RNA

Physical Interaction

Assay

References

Mcr - mir-184

luminiscence technology, phenotype-based detection assay, immunohistochemistry

(Lim et al., 2018, Sharifkhodaei et al., 2016)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Mcr

(Ward et al., 2003)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0267488

MIST (protein-protein) - An integrated Molecular Interaction Database

44071

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

(Waterhouse et al., 2007)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-30

Cytogenetic map

28E1-28E3

Sequence location

2L:8,074,117..8,082,982 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

28E1-28E3

Limits computationally determined from genome sequence between P{EP}poe^EP737 and P{lacW}l(2)k16919^k16919&P{lacW}Bsg^k09030

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

28D-28E

(Crowley, 1997.2.6, Crowley et al., 1993)

Experimentally Determined Recombination Data

Location

2-30

(FlyBase, 2016)

2-26.4

(Comeron et al., 2012)

2-35--38

(Ward et al., 2003)

(Crowley, 1997.2.6)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

606765

w^*; PBac{U6-Mcr.gRNA}VK00027/TM6B, Tb¹

15997

y¹ w^67c23; P{EPgy2}Mcr^EY07421/CyO

65896

y¹ sc^* v¹ sev²¹; P{TRiP.HMC06158}attP40

Korea

10894

P{EP}Mcr^G2308

Kyoto

111715

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{EPgy2}Mcr^EY07421 P{neoFRT}40A/CyO y⁺

NIG-Fly

M2L-1275

y¹ w¹¹¹⁸; Mcr^SK5 P{neoFRT}40A/CyO-Tb

M2L-1276

y¹ w¹¹¹⁸; Mcr^SK6 P{neoFRT}40A/CyO-Tb

2LG-1016

y² VhaAC39-1^cho-1 v¹; P{U6-Mcr.NIG.sgRNA}attP40/CyO

VDRC

v100197

P{KK104742}VIE-260B

v2785

w¹¹¹⁸; P{GD988}v2785

Genomic Clones (28)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (92)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD23292

Other clones

DPiM_LD23292

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0267488

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Hall et al., 2014)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Mcr is required cell autonomously for the correct membrane localisation of several septate junction components and is required for epithelial barrier formation.

(Bätz et al., 2014)

Mcr is required cell-autonomously for septate junction organisation in embryonic epithelia and larval wing imaginal discs and is required for paracellular barrier function.

(Hall et al., 2014)

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. However, the dsRNA treatment does not affect phagocytosis of either Escherichia coli, Staphylococcus aureus or latex beads.

(Stroschein-Stevenson et al., 2006)

P-element insertions P{lacW}Trf¹ and P{lacW}Trf² (which lie within 50bp of each other at 28D--28E) map approximately 3kb proximal to 3' end of Mcr transcription unit.

(Crowley et al., 1993)

Transcription unit defined as part of a molecular analysis of Trf.

(Crowley et al., 1993)

Relationship to Other Genes

Source for database merge of

Source for merge of: Mcr CG7586

(Bayraktaroglu, 2000.4.3)

Source for merge of: Mcr E(br)155

(Hall et al., 2014)

Additional comments

E(br)155 comprises two closely linked mutations corresponding to SP1070 and an anonymous gene in region 26.

(Zhang et al., 2008)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

CG7586

(National Institute of Genetics Fly Stocks, 2022-, Hosono et al., 2015, Jaspers et al., 2012, Keleman et al., 2009.8.5, Perkins et al., 2009, Schiess et al., 2009, Stabell et al., 2006, Anholt and Mackay, 2001)

DmTEP6

(Bou Aoun et al., 2011)

E(br)155

(Zhang and Ward, 2009, Zhang et al., 2008, Zhang et al., 2007)

TEP6

(Tafesh-Edwards and Eleftherianos, 2023, Younes et al., 2020, Bou Aoun et al., 2011)

TEP6mel

(Bou Aoun et al., 2011)

Tep6

(Liegeois and Ferrandon, 2022, Eleftherianos and Sachar, 2020, Lu et al., 2020, Melcarne et al., 2019, Shokal and Eleftherianos, 2017, Bou Aoun et al., 2011, Bou Aoun et al., 2008, Waterhouse et al., 2007)

TepVI

(Christophides et al., 2002)

anon-28DE

dTEPVI

(Lagueux, 2000.3.15, Lagueux et al., 2000)

luf

(Bätz et al., 2014)

mcr

(Rouka et al., 2021, Lin et al., 2017, Guenin et al., 2010)

α-2-Mrp

Name Synonyms

E(br)155

Enhancer of broad

(Zhang et al., 2007)

Enhancer of broad 155

(Zhang et al., 2008)

Macroglobulin complement related

(Bier and Guichard, 2012, Anonymous, 2006)

Macroglobulin complement-related

(Jaspers et al., 2012, Crowley, 1997.7.14)

TEP

(Erturk-Hasdemir and Silverman, 2005)

Tep6

Thiolester containing protein VI

(Lagueux et al., 2000)

luftschlange

(Bätz et al., 2014)

α-2-macroglobulin related

Secondary FlyBase IDs

FBgn0087010
FBgn0041178
FBgn0031984
FBgn0014086
FBgn0020240
FBgn0264800
FBgn0069452

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44071

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VLT3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VLT3

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VLT3

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44071/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267488

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267488

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7586

MARRVEL_MODEL - MARRVEL (model organism gene)

44071

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0267488

DroID - A comprehensive database of gene and protein interactions.

FBgn0267488

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mcr_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020240

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0267488

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7586

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0267488

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009375

MIST (protein-protein) - An integrated Molecular Interaction Database

44071

References (91)

The following external sites may use different assemblies or annotations than FlyBase.

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