FB2022_03, released June 9, 2022

Gene: Dmel\slmb

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General Information
Symbol
Dmel\slmb
Species
D. melanogaster
Name
supernumerary limbs
Annotation Symbol
CG3412
Feature Type
protein_coding_gene
FlyBase ID
FBgn0283468
Gene Model Status
Current
Stock Availability
25 publicly available
Gene Summary
supernumerary limbs (slmb) encodes an essential, conserved F-box protein and a component of the SCF (Skp/Cullin/F-box) E3 ubiquitin-ligase, providing substrate specificity to the SCF. It negatively regulates multiple signaling pathways and cellular processes by promoting the proteasome-mediated degradation or cleavage of its targets, including the products of arm and ci (Wg and Hh signaling), SAK, per and Cap-H2. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

slimb, ica, shv, shiva, β-TrCP

Key Links
Genomic Location
Cytogenetic map
93B13-93C1
Sequence location
Recombination map
3-70
RefSeq locus
NT_033777 REGION:21119252..21127485
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (48 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
enables beta-catenin destruction complex binding
inferred from direct assay
(Schaefer et al., 2020)
enables molecular adaptor activity
inferred from physical interaction with FLYBASE:ci; FB:FBgn0004859, FLYBASE:Cul1; FB:FBgn0015509
(Smelkinson et al., 2007)
enables phosphoprotein binding
inferred from physical interaction with FLYBASE:ci; FB:FBgn0004859
(Jia et al., 2005)
enables ubiquitin ligase-substrate adaptor activity
inferred from direct assay
(Li et al., 2020, Wong et al., 2013)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables protein dimerization activity
inferred from electronic annotation with InterPro:IPR021977
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables ubiquitin ligase-substrate adaptor activity
inferred from biological aspect of ancestor with PANTHER:PTN002891465
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (40 terms)
Terms Based on Experimental Evidence (38 terms)
CV Term
Evidence
References
involved_in chorion-containing eggshell formation
inferred from mutant phenotype
(Muzzopappa and Wappner, 2005)
involved_in circadian rhythm
inferred from mutant phenotype
(Grima et al., 2002)
inferred from physical interaction with FLYBASE:dco; FB:FBgn0002413
(Grima et al., 2002)
inferred from physical interaction with FLYBASE:per; FB:FBgn0003068
(Grima et al., 2002)
involved_in dorsal appendage formation
inferred from mutant phenotype
(Muzzopappa and Wappner, 2005)
involved_in establishment of epithelial cell polarity
inferred from mutant phenotype
(Morais-de-Sá et al., 2014)
involved_in germarium-derived egg chamber formation
inferred from mutant phenotype
(Muzzopappa and Wappner, 2005)
involved_in germarium-derived female germ-line cyst encapsulation
inferred from mutant phenotype
(Muzzopappa and Wappner, 2005)
involved_in germarium-derived female germ-line cyst formation
inferred from mutant phenotype
(Muzzopappa and Wappner, 2005)
involved_in locomotor rhythm
inferred from mutant phenotype
(Grima et al., 2002)
involved_in negative regulation of antimicrobial peptide production
inferred from mutant phenotype
(Khush et al., 2002)
involved_in negative regulation of apoptotic process
inferred from mutant phenotype
(Fereres et al., 2013)
involved_in negative regulation of canonical Wnt signaling pathway
inferred from mutant phenotype
(Roberts et al., 2012, Swarup and Verheyen, 2011, Jiang and Struhl, 1998)
involved_in negative regulation of cell population proliferation
inferred from mutant phenotype
(Skwarek et al., 2014)
involved_in negative regulation of glial cell proliferation
inferred from genetic interaction with FLYBASE:ago; FB:FBgn0041171
(Ho et al., 2009)
involved_in negative regulation of hippo signaling
inferred from mutant phenotype
(Tokamov et al., 2021, Fulford et al., 2019, Zhang et al., 2015)
inferred from genetic interaction with FLYBASE:POSH; FB:FBgn0040294
(Ma et al., 2018)
involved_in negative regulation of insulin receptor signaling pathway
inferred from genetic interaction with FLYBASE:InR; FB:FBgn0283499
(Wong et al., 2013)
involved_in negative regulation of nurse cell apoptotic process
inferred from mutant phenotype
(Muzzopappa and Wappner, 2005)
involved_in negative regulation of peptidoglycan recognition protein signaling pathway
inferred from mutant phenotype
(Khush et al., 2002)
involved_in negative regulation of protein kinase B signaling
inferred from physical interaction with FLYBASE:Akt; FB:FBgn0010379
(Wong et al., 2013)
involved_in negative regulation of smoothened signaling pathway
inferred from genetic interaction with FLYBASE:Pka-C1; FB:FBgn0000273
(Smelkinson et al., 2007)
inferred from genetic interaction with FLYBASE:TER94; FB:FBgn0286784
(Zhang et al., 2013)
inferred from direct assay
(Li et al., 2020)
inferred from mutant phenotype
(Zhou et al., 2015, Wang et al., 1999, Jiang and Struhl, 1998, Ohlmeyer and Kalderon, 1998)
inferred from genetic interaction with FLYBASE:smo; FB:FBgn0003444
(Smelkinson and Kalderon, 2006)
involved_in oocyte axis specification
inferred from mutant phenotype
(Morais-de-Sá et al., 2014)
involved_in oocyte localization involved in germarium-derived egg chamber formation
inferred from mutant phenotype
(Muzzopappa and Wappner, 2005)
involved_in ovarian follicle cell development
inferred from mutant phenotype
(Muzzopappa and Wappner, 2005)
involved_in ovarian follicle cell stalk formation
inferred from mutant phenotype
(Muzzopappa and Wappner, 2005)
involved_in positive regulation of border follicle cell migration
inferred from mutant phenotype
(Mathieu et al., 2007)
involved_in positive regulation of neuron remodeling
inferred from mutant phenotype
(Wong et al., 2013)
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process
inferred from mutant phenotype
(Ribeiro et al., 2014, Roberts et al., 2012, Ohlmeyer and Kalderon, 1998)
inferred from direct assay
(Zhang et al., 2015)
involved_in positive regulation of protein catabolic process
inferred from genetic interaction with FLYBASE:POSH; FB:FBgn0040294, FLYBASE:crb; FB:FBgn0259685
(Ma et al., 2018)
involved_in positive regulation of synaptonemal complex assembly
inferred from mutant phenotype
(Barbosa et al., 2021)
involved_in positive regulation of Toll signaling pathway
inferred from mutant phenotype
(Daigneault et al., 2013)
involved_in protein K11-linked ubiquitination
inferred from direct assay
(Zhang et al., 2013)
involved_in protein polyubiquitination
inferred from mutant phenotype
(Ribeiro et al., 2014)
inferred from direct assay
(Zhang et al., 2015, Swarup and Verheyen, 2011)
involved_in protein ubiquitination
inferred from mutant phenotype
(Tokamov et al., 2021, Wong et al., 2013)
inferred from direct assay
(Li et al., 2020, Zhou et al., 2015)
involved_in proteolysis
inferred from mutant phenotype
(Noureddine et al., 2002)
involved_in regulation of centriole replication
inferred from direct assay
(Rogers et al., 2009)
involved_in regulation of chromosome condensation
inferred from mutant phenotype
(Buster et al., 2013)
involved_in regulation of establishment of cell polarity
inferred from mutant phenotype
(Skwarek et al., 2014)
involved_in regulation of mitotic nuclear division
inferred from mutant phenotype
(Muzzopappa and Wappner, 2005)
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
inferred from mutant phenotype
(Tokamov et al., 2021)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in protein polyubiquitination
inferred from biological aspect of ancestor with PANTHER:PTN002891465
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in proteolysis
inferred from biological aspect of ancestor with PANTHER:PTN000520494
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in regulation of cell cycle
inferred from biological aspect of ancestor with PANTHER:PTN000520494
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in regulation of proteasomal protein catabolic process
inferred from biological aspect of ancestor with PANTHER:PTN000520494
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
inferred from sequence model
(FlyBase Curators, 2011-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
part_of SCF ubiquitin ligase complex
inferred from direct assay
(Barbosa et al., 2021, Ribeiro et al., 2014, Wong et al., 2013, Zhang et al., 2013, Smelkinson et al., 2007, Bocca et al., 2001)
inferred from mutant phenotype
(Roberts et al., 2012)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000520494
(assigned by GO_Central )
(Gaudet et al., 2011)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000520494
(assigned by GO_Central )
(Gaudet et al., 2011)
part_of SCF ubiquitin ligase complex
inferred from biological aspect of ancestor with PANTHER:PTN002891465
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
supernumerary limbs (slmb) encodes an essential, conserved F-box protein and a component of the SCF (Skp/Cullin/F-box) E3 ubiquitin-ligase, providing substrate specificity to the SCF. It negatively regulates multiple signaling pathways and cellular processes by promoting the proteasome-mediated degradation or cleavage of its targets, including the products of arm and ci (Wg and Hh signaling), SAK, per and Cap-H2. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
F BOX AND WD DOMAIN PROTEINS -
F box and WD domain (FBXW) proteins are F box proteins which contain WD40 repeats. F box proteins are the variable substrate adapters for Skp, Cullin, F-box (SCF) E3 ubiquitin ligase complexes. (Adapted from FBrf0225076).
SCF-SLMB UBIQUITIN LIGASE COMPLEX -
SCF-slmb ubiquitin ligase complex ubiquitinates proteins, targeting them for degradation by the proteasome. (FBrf0217546).
Pathway (FlyBase)
Negative Regulators of Wnt-TCF Signaling Pathway -
Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
Negative Regulators of Insulin-like Receptor Signaling Pathway -
Negative regulators of the Insulin-like Receptor signaling pathway suppress Insulin-like receptor (InR) activation or the activity of intracellular effectors. (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
Negative Regulators of Hippo Signaling Pathway -
The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).
Negative Regulators of Hedgehog Signaling Pathway -
Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.
Positive Regulators of Toll-NF-κB Signaling Pathway -
In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. Positive regulators of Toll-NF-κB Signaling lead to the increased nuclear accumulation of the NF-κB proteins and regulated expression of target genes. (Adapted from FBrf0091014 and FBrf0223077).
Negative Regulators of Imd Signaling Pathway -
Negative regulators of the immune deficiency (Imd) pathway result in the decreased activity of the NFκB-like transcription factor Rel in the nucleus. Negative regulators are important in preventing damage to the host from over-activation of the pathway; preventing inappropriate triggering or terminating the response. (Adapted from FBrf0224587 and FBrf0238555.)
Summary (Interactive Fly)

beta-transducin family Trp-Asp repeats family - subunit of a multi-protein complex that targets proteins for degradation by the ubiquitin-proteasome pathway - an important regulator of Wingless, Hedgehog and Dorsal pathways

Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\slmb for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry A0A0B4KHK1)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Stop-codon suppression (UAA) postulated; FBrf0216884.

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene
gene
(Menut et al., 2007)
gene_with_stop_codon_read_through
(Jungreis et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
slmb-RA
FBtr0084032
NM_079706
3492
510
slmb-RB
FBtr0330049
NM_001275873
3492
597
slmb-RC
FBtr0333931
NM_001275872
2549
510
Additional Transcript Data and Comments
Reported size (kB)

4.0, 3.0, 2.6 (northern blot)

(Miletich and Limbourg-Bouchon, 2000)

2.154 (compiled cDNA)

(Jiang and Struhl, 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
slmb-PA
FBpp0083434
59.0
510
7.67
Q9VDE3
NP_524430
AAF55853
slmb-PB
FBpp0303082
68.6
597
5.18
A0A0B4KHK1
NP_001262802
AGB96182
slmb-PC
FBpp0306059
59.0
510
7.67
Q9VDE3
NP_001262801
AGB96181
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

510 aa isoforms: slmb-PA, slmb-PC
Additional Polypeptide Data and Comments
Reported size (kDa)

510 (aa)

(Jiang and Struhl, 1998)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\slmb using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:
 low
high 
Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.
Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:
 low
high 
as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
organism
(Miletich and Limbourg-Bouchon, 2000)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
anterior endoderm anlage
(Fisher et al., 2012)
mesectoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
dorsal ectoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
central brain primordium
(Fisher et al., 2012)
dorsal epidermis primordium
(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
tracheal primordium
(Fisher et al., 2012)
ventral epidermis primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic ventral epidermis
(Fisher et al., 2012)
embryonic/larval nervous system
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | male
organism
(Miletich and Limbourg-Bouchon, 2000)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of SCF ubiquitin ligase complex
inferred from direct assay
(Barbosa et al., 2021, Ribeiro et al., 2014, Wong et al., 2013, Zhang et al., 2013, Smelkinson et al., 2007, Bocca et al., 2001)
inferred from mutant phenotype
(Roberts et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\slmb in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 37 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 31 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of slmb
Transgenic constructs containing regulatory region of slmb
Aberrations (Deficiencies and Duplications) ( 4 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone
lethal
lethal, with Scer\GAL4Mef2.PR
lethal (with Df(3R)ED6058)
lethal (with Df(3R)e-R1)
lethal (with Df(3R)Exel6272)
lethal (with slmb8A6-5)
lethal (with slmb9H4-17)
lethal | heat sensitive, with Scer\GAL4da.PU, brpMI02987-GFSTF.0
lethal | heat sensitive, with Scer\GAL4nSyb.PS, brpMI02987-GFSTF.0
lethal | recessive
lethal - all die before end of larval stage (with Df(3R)e-R1)
lethal - all die before end of larval stage (with slmb8)
lethal - all die before end of larval stage (with slmb41)
lethal - all die before end of larval stage (with slmbP245)
lethal - all die before end of larval stage | recessive
lethal - all die before end of pupal stage
lethal - all die before end of pupal stage, with Scer\GAL4pnr-MD237
lethal - all die before end of pupal stage | recessive
lethal - all die before end of pupal stage | recessive | cold sensitive
lethal - all die during embryonic stage | heat sensitive, with Scer\GAL4Tub.PU, dlg1MI06353-GFSTF.0
lethal - all die during embryonic stage | heat sensitive, with α-CatMI02577-GFSTF.0, Scer\GAL4Act.PU
lethal - all die during embryonic stage | recessive
lethal - all die during first instar larval stage
lethal - all die during first instar larval stage, with slmb8
lethal - all die during first instar larval stage, with slmbL.hs
lethal - all die during third instar larval stage | heat sensitive, with slmb8
lethal - all die during third instar larval stage | heat sensitive, with slmbL.hs
viable, with Scer\GAL4nullo.PG
viable, with Scer\GAL4tin.CΔ4
viable, with Scer\GAL4Tub.PU
Sterility
Allele
female semi-sterile, with Scer\GAL4VP16.nos.UTR
female sterile, with Scer\GAL4VP16.nos.UTR
fertile, with Scer\GAL4nullo.PG
fertile, with Scer\GAL4Tub.PU
Other Phenotypes
Allele
abnormal circadian rhythm
abnormal circadian rhythm, with Scer\GAL41118
abnormal circadian rhythm, with slmb8
abnormal circadian rhythm, with slmbL.hs
abnormal developmental rate | gastrula stage, with cherCPTI001399
abnormal learning
abnormal learning | heat sensitive, with Scer\GAL4117Y, dncMI03415-GFSTF.2
abnormal locomotor behavior | adult stage, with PitsMI02926-DH.PT-GFSTF.1, Scer\GAL4Act5C.PI
abnormal locomotor behavior | adult stage | progressive, with PitsMI02926-DH.PT-GFSTF.1, Scer\GAL4Act5C.PI
abnormal locomotor behavior | adult stage | progressive, with PitsMI02926-DH.PT-GFSTF.1, Scer\GAL4nSyb.PS
abnormal locomotor behavior | heat sensitive, with Scer\GAL4nSyb.PS, brpMI02987-GFSTF.0
abnormal locomotor rhythm, with Scer\GAL4tim.PE
abnormal locomotor rhythm, with Scer\GAL4tim.UAS
abnormal locomotor rhythm, with slmb8
abnormal locomotor rhythm, with slmbL.hs
abnormal memory
abnormal memory | conditional, with Scer\GAL4Mef2.247, mengMI03008-GFSTF.1
abnormal mitotic cell cycle
abnormal mitotic cell cycle | germline clone
abnormal neuroanatomy
abnormal neuroanatomy | adult stage | progressive, with PitsMI02926-DH.PT-GFSTF.1, Scer\GAL4nSyb.PS
abnormal neuroanatomy | pupal stage | recessive | somatic clone
abnormal neuroanatomy | pupal stage | somatic clone
abnormal neuroanatomy | somatic clone | third instar larval stage
abnormal neurophysiology, with Scer\GAL4ey.PU, brpMI02987-GFSTF.0
abnormal neurophysiology | heat sensitive, with Scer\GAL4nSyb.PS, brpMI02987-GFSTF.0
abnormal planar polarity, with Rab23EYFP, Scer\GAL4en.PU
abnormal size, with Scer\GAL4sd.PU
decreased occurrence of cell division
decreased size | adult stage | female limited, with Scer\GAL4VP16.nos.UTR
increased cell death
increased cell death, with Scer\GAL4sd.PU
increased cell death | germline clone
increased cell death | third instar larval stage, with Scer\GAL4pnr-MD237
increased cell size
increased size | adult stage, with Scer\GAL4GMR.PU
neoplasia | somatic clone
short lived, with PitsMI02926-DH.PT-GFSTF.1, Scer\GAL4Act5C.PI
short lived, with PitsMI02926-DH.PT-GFSTF.1, Scer\GAL4nSyb.PS
some die during embryonic stage | recessive
some die during larval stage (with Df(3R)e-R1)
some die during larval stage (with slmb8)
some die during larval stage (with slmb41)
some die during larval stage (with slmbP245)
some die during larval stage | recessive
some die during pupal stage
some die during pupal stage, with Scer\GAL4pnr-MD237
some die during pupal stage | recessive
some die during pupal stage | recessive | cold sensitive
visible, with Scer\GAL4dpp.blk1
visible, with α-CatMI02577-GFSTF.0, Scer\GAL4ey.PU
visible | adult stage, with Scer\GAL4GMR.PFa
visible | adult stage, with Scer\GAL4GMR.PU
visible | adult stage, with Scer\GAL4pnr-MD237
visible | dominant
visible | recessive | somatic clone
wild-type
Phenotype manifest in
Allele
1st posterior cell, with Scer\GAL4dpp.blk1
actomyosin contractile ring | embryonic stage 5, with cherCPTI001399
actomyosin contractile ring | embryonic stage 5, with FimCPTI100066
adult abdomen, with Scer\GAL4pnr-MD237
adult cuticle & head | dorsal | somatic clone
adult thorax, with Scer\GAL4pnr-MD237
anterior crossvein, with Scer\GAL4dpp.blk1
axon | adult stage | decreased number, with PitsMI02926-DH.PT-GFSTF.1, Scer\GAL4Act5C.PI
axon | adult stage | decreased number, with PitsMI02926-DH.PT-GFSTF.1, Scer\GAL4nSyb.PS
axon | pupal stage | somatic clone
border follicle cell | somatic clone
centrosome & neuroblast
centrosome | ectopic
chromatin | somatic clone
dorsal appendage | dorsal | somatic clone
dorsal appendage | somatic clone
dorsal appendage | somatic clone | ventral
egg | germline clone
egg chamber, with slmb8, slmb9H4-17
egg chamber, with slmb8, slmbL.hs
egg chamber, with slmb9H4-17, slmbL.hs
egg chamber, with slmb9H4-17/slmb8
egg chamber (with slmb8), with slmbL.hs
egg chamber (with slmb9H4-17), with slmbL.hs
egg chamber | decreased number, with Scer\GAL4VP16.nos.UTR
egg chamber | germline clone
egg chamber | somatic clone
egg chorion | germline clone
egg operculum | somatic clone
embryo, with Rab8EGFP, Scer\GAL4VP16.mat.αTub67C
embryonic/larval brain | heat sensitive, with Scer\GAL4Tub.PU, dlg1MI06353-GFSTF.0
embryonic/larval brain | larval stage
embryonic/larval fat body | third instar larval stage, with Scer\GAL4SPARC-MI00329-GAL4
embryonic/larval midgut | heat sensitive, with Scer\GAL4Tub.PU, dlg1MI06353-GFSTF.0
embryonic/larval neuromuscular junction | heat sensitive, with Scer\GAL4Tub.PU, dlg1MI06353-GFSTF.0
embryonic/larval salivary gland | third instar larval stage, with Scer\GAL4hs.PU
embryonic epidermis | embryonic stage 5, with cherCPTI001399
embryonic epidermis | embryonic stage 5, with FimCPTI100066
extracellular matrix | third instar larval stage, with Scer\GAL4SPARC-MI00329-GAL4
eye, with Scer\GAL4GMR.PFa
eye, with Scer\GAL4GMR.PU
eye, with α-CatMI02577-GFSTF.0, Scer\GAL4ey.PU
eye | somatic clone
eye disc | somatic clone
female germ cell nucleus | germline clone
female germline stem cell | germline clone
filamentous actin | embryonic stage 5, with cherCPTI001399
filamentous actin | embryonic stage 5, with FimCPTI100066
follicle cell | germline clone
gamma Kenyon cell | pupal stage | somatic clone
gamma Kenyon cell | somatic clone | third instar larval stage
germarium region 2b & germline cyst | somatic clone
germarium region 3 & germline cyst | somatic clone
haltere
haltere | ectopic
karyosome, with Scer\GAL4VP16.nos.UTR
larva | first instar larval stage
larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone
leg
leg | distal
leg | distal | ectopic
leg | ectopic | somatic clone
leg | somatic clone
macrochaeta & wing margin
macrochaeta | ectopic
marginal nerve, with PitsMI02926-DH.PT-GFSTF.1, Scer\GAL4Act5C.PI
marginal nerve, with PitsMI02926-DH.PT-GFSTF.1, Scer\GAL4nSyb.PS
mitotic cell cycle
mitotic cell cycle & neuroblast
neuroblast | somatic clone
nurse cell | germline clone
nurse cell | increased number, with Scer\GAL4VP16.nos.UTR
nurse cell | somatic clone | increased number
oocyte | germline clone
oocyte | somatic clone
oocyte | somatic clone | increased number
ovary, with Scer\GAL4VP16.nos.UTR
polytene chromosome, with Scer\GAL4hs.PU
ring canal | germline clone | increased number
stalk follicle cell | somatic clone
synaptonemal complex | conditional, with Scer\GAL4nos.PG, vtdαTub84B.EGFP, vtdex3
synaptonemal complex | oogenesis | oogenesis, with Scer\GAL4VP16.nos.UTR
ventral thoracic disc
wing
wing, with Scer\GAL4sd.PU
wing | ectopic
wing | ectopic | somatic clone
wing | somatic clone
wing disc
wing disc, with Scer\GAL4sd.PU
wing disc | heat sensitive, with Scer\GAL4Tub.PU, dlg1MI06353-GFSTF.0
wing disc | somatic clone
wing disc | third instar larval stage, with Scer\GAL4pnr-MD237
wing margin bristle | ectopic | somatic clone
wing vein | ectopic | somatic clone
wing vein | somatic clone
wing vein L3 | ectopic | somatic clone
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\BTRC
13 of 15
Yes
Yes
1  
Hsap\FBXW11
11 of 15
No
Yes
1  
Hsap\DAW1
1 of 15
No
No
Hsap\FBXW7
1 of 15
No
No
Hsap\WDR64
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Btrc
14 of 15
Yes
Yes
Mmus\Fbxw11
10 of 15
No
Yes
Mmus\Daw1
1 of 15
No
No
Mmus\Fbxw7
1 of 15
No
No
Rattus norvegicus (Norway rat) (2)
Rnor\Btrc
12 of 13
Yes
Yes
Rnor\Fbxw11
5 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
Xtro\btrc
9 of 12
Yes
Yes
Xtro\daw1
1 of 12
No
No
Xtro\fbxw7
1 of 12
No
No
Danio rerio (Zebrafish) (5)
Drer\fbxw11a
11 of 15
Yes
Yes
Drer\fbxw11b
11 of 15
Yes
Yes
Drer\daw1
1 of 15
No
No
Drer\fbxw7
1 of 15
No
No
Drer\traf7
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\lin-23
12 of 15
Yes
Yes
Cele\sel-10
2 of 15
No
No
Cele\Y43F4B.9
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (5)
Scer\CDC4
2 of 15
Yes
No
Scer\MET30
2 of 15
Yes
No
Scer\CAF4
1 of 15
No
Yes
Scer\MDV1
1 of 15
No
Yes
Scer\RRT13
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (3)
Spom\pof11
8 of 12
Yes
Yes
Spom\pof1
2 of 12
No
Yes
Spom\pop2
1 of 12
No
No
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (5)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
ago
3 of 10
CG7568
2 of 10
CG10459
1 of 10
WDY
1 of 10
wmd
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Evidence
    References
    slmbGD10682
    model of  Menkes disease
    CEA
    (Zhang et al., 2020)
    slmbKK108569
    model of  Menkes disease
    CEA
    (Zhang et al., 2020)
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 3 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    BTRC; beta-transducin repeat containing E3 ubiquitin protein ligase
    13 of 15
    603482
         
        FBXW11; F-box and WD repeat domain containing 11
        11 of 15
        605651
           
          Functional Complementation Data
          Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
          Interactions
          Summary of Physical Interactions
          esyN Network Diagram
          Show neighbor-neighbor interactions:
          Select Layout:
          Legend:
          Protein
          RNA
          Selected Interactor(s)
          Interactions Browser
          View in Interactions Browser
          Please see the Physical Interaction reports below for full details
          protein-protein
          Physical Interaction
          Assay
          References
          slmb
          anti tag coimmunoprecipitation, anti tag western blot
          (Smelkinson et al., 2007)
          slmb - Akt
          anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, anti tag western blot
          (Wong et al., 2013)
          slmb - Blimp-1
          anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
          (Rhee et al., 2014)
          slmb - CG13088
          experimental knowledge based
          (Guruharsha et al., 2011)
          slmb - CG32219
          experimental knowledge based
          (Guruharsha et al., 2011)
          slmb - CG7407
          experimental knowledge based
          (Guruharsha et al., 2011)
          slmb - Cap-H2
          anti tag coimmunoprecipitation, western blot, anti tag western blot, anti bait coimmunoprecipitation
          (Nguyen et al., 2015, Buster et al., 2013)
          slmb - CkIα
          anti tag coimmunoprecipitation, anti tag western blot
          (Fulford et al., 2019)
          slmb - Cul1
          anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot, western blot
          (Barbosa et al., 2021, Wong et al., 2013, Zhang et al., 2013, Smelkinson et al., 2007)
          slmb - Debcl
          anti tag coimmunoprecipitation, anti tag western blot, proximity ligation assay, fluorescence microscopy
          (Colin et al., 2014)
          slmb - Dlish
          anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot
          (Wang et al., 2019, Zhang et al., 2016)
          slmb - E(spl)m8-HLH
          experimental knowledge based
          (Guruharsha et al., 2011)
          slmb - E2f1
          pull down, autoradiography
          (Heriche et al., 2003)
          slmb - E2f2
          pull down, autoradiography
          (Heriche et al., 2003)
          slmb - HIV-1\Vpu
          two hybrid, anti bait coimmunoprecipitation, anti tag western blot
          (Marchal et al., 2012)
          slmb - Hipk
          enzymatic study, autoradiography, pull down, anti tag western blot
          (Swarup and Verheyen, 2011)
          slmb - Mbs
          experimental knowledge based
          (Guruharsha et al., 2011)
          slmb - Mer
          anti tag coimmunoprecipitation, anti tag western blot
          (Tokamov et al., 2021)
          slmb - PR-Set7
          anti tag coimmunoprecipitation, western blot
          (Zouaz et al., 2018)
          slmb - Roc1a
          anti tag coimmunoprecipitation, western blot, anti tag western blot
          (Wong et al., 2013, Zhang et al., 2013)
          slmb - SAK
          anti tag coimmunoprecipitation, western blot, pull down, anti tag western blot, anti bait coimmunoprecipitation, peptide massfingerprinting
          (Boese et al., 2018, Klebba et al., 2015, Klebba et al., 2015, Cunha-Ferreira et al., 2013, Klebba et al., 2013, Dzhindzhev et al., 2010, Rogers et al., 2009)
          slmb - SkpA
          anti tag coimmunoprecipitation, anti tag western blot, western blot, anti bait coimmunoprecipitation, experimental knowledge based, Identification by mass spectrometry
          (Barbosa et al., 2021, Xiao et al., 2015, Wong et al., 2013, Zhang et al., 2013, Guruharsha et al., 2011, Rogers et al., 2009, Silva et al., 2007)
          slmb - SkpC
          experimental knowledge based
          (Guruharsha et al., 2011)
          slmb - Smn
          anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot, western blot
          (Gray et al., 2018)
          slmb - TER94
          anti tag coimmunoprecipitation, anti tag western blot
          (Zhang et al., 2013)
          slmb - arm
          anti tag coimmunoprecipitation, western blot
          (White et al., 2018)
          slmb - ci
          pull down, autoradiography, anti tag western blot, enzymatic study, peptide massfingerprinting
          (Zhang et al., 2013, Smelkinson et al., 2007, Smelkinson and Kalderon, 2006, Jia et al., 2005, Dai et al., 2003)
          slmb - dbr
          anti bait coimmunoprecipitation, anti tag western blot, pull down, autoradiography
          (Dai et al., 2003)
          slmb - ds
          anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting
          (Kwon et al., 2013, Kwon et al., 2013)
          slmb - en
          anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
          (Rhee et al., 2014)
          slmb - ex
          anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot, pull down, western blot
          (Fulford et al., 2019, Wang et al., 2019, Zhang et al., 2015, Ribeiro et al., 2014)
          slmb - gcm
          anti tag coimmunoprecipitation, anti tag western blot
          (Xi et al., 2017, Ho et al., 2009)
          slmb - hpo
          anti tag coimmunoprecipitation, anti tag western blot
          (Tokamov et al., 2021)
          slmb - kibra
          anti tag coimmunoprecipitation, anti tag western blot
          (Tokamov et al., 2021)
          slmb - morgue
          experimental knowledge based
          (Guruharsha et al., 2011)
          slmb - nerfin-1
          experimental knowledge based, anti tag coimmunoprecipitation, anti tag western blot
          (Lin et al., 2018, Guruharsha et al., 2011)
          slmb - par-1
          anti bait coimmunoprecipitation, anti tag western blot
          (Lee et al., 2012)
          slmb - per
          pull down, anti tag western blot
          (Chiu et al., 2008)
          slmb - vri
          experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting
          (Rhee et al., 2014, Guruharsha et al., 2011)
          slmb - wts
          anti tag coimmunoprecipitation, anti tag western blot
          (Tokamov et al., 2021)
          RNA-protein
          Physical Interaction
          Assay
          References
          slmb - Atx2
          anti bait coimmunoprecipitation, primer specific pcr
          (Zhang et al., 2013)
          Summary of Genetic Interactions
          esyN Network Diagram
          esyN Network Key:
          Suppression
          Enhancement
          View in Interactions Browser
          Please look at the allele data for full details of the genetic interactions
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          slmb
          suppressible
          aPKC
          (Morais-de-Sá et al., 2014, Skwarek et al., 2014)
          slmb
          suppressible
          ATP7
          (Zhang et al., 2020)
          Pits|slmb
          suppressible
          CkIα
          (Marcogliese et al., 2022)
          slmb
          suppressible
          cnc
          (Zhang et al., 2020)
          slmb
          enhanceable
          CSN5
          (Muzzopappa and Wappner, 2005)
          cnc|slmb
          suppressible
          Ctr1A
          (Zhang et al., 2020)
          slmb
          suppressible
          Ctr1A
          (Zhang et al., 2020)
          slmb
          enhanceable
          Cul1
          (Muzzopappa and Wappner, 2005)
          slmb
          suppressible
          E2f1
          (Heriche et al., 2003)
          ago|slmb
          suppressible
          gcm
          (Ho et al., 2009)
          slmb
          enhanceable
          Mical
          (Wong et al., 2013)
          slmb
          enhanceable
          RYBP
          (Fereres et al., 2013)
          slmb
          suppressible
          smo
          (Theodosiou et al., 1998)
          slmb
          suppressible
          yki
          (Skwarek et al., 2014)
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          CG9003
          enhanceable
          slmb
          (Meltzer et al., 2019)
          crb
          enhanceable
          slmb
          (Skwarek et al., 2014)
          Debcl
          enhanceable
          slmb
          (Colin et al., 2014)
          dpp
          suppressible
          slmb
          (Muzzopappa and Wappner, 2005)
          glu|Cap-H2
          enhanceable
          slmb
          (Buster et al., 2013)
          Mical
          enhanceable
          slmb
          (Wong et al., 2013)
          nerfin-1
          enhanceable
          slmb
          (Lin et al., 2018)
          nerfin-1
          suppressible
          slmb
          (Lin et al., 2018)
          RYBP
          enhanceable
          slmb
          (Fereres et al., 2013)
          External Data
          Linkouts
          MIST (genetic) - An integrated Molecular Interaction Database
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Pathways
          Signaling Pathways (FlyBase)
          Negative Regulators of Wnt-TCF Signaling Pathway -
          Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
          Negative Regulators of Insulin-like Receptor Signaling Pathway -
          Negative regulators of the Insulin-like Receptor signaling pathway suppress Insulin-like receptor (InR) activation or the activity of intracellular effectors. (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
          Negative Regulators of Hippo Signaling Pathway -
          The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).
          Negative Regulators of Hedgehog Signaling Pathway -
          Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.
          Positive Regulators of Toll-NF-κB Signaling Pathway -
          In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. Positive regulators of Toll-NF-κB Signaling lead to the increased nuclear accumulation of the NF-κB proteins and regulated expression of target genes. (Adapted from FBrf0091014 and FBrf0223077).
          Negative Regulators of Imd Signaling Pathway -
          Negative regulators of the immune deficiency (Imd) pathway result in the decreased activity of the NFκB-like transcription factor Rel in the nucleus. Negative regulators are important in preventing damage to the host from over-activation of the pathway; preventing inappropriate triggering or terminating the response. (Adapted from FBrf0224587 and FBrf0238555.)
          Metabolic Pathways
          External Data
          Genomic Location and Detailed Mapping Data
          Chromosome (arm)
          3R
          Recombination map
          3-70
          Cytogenetic map
          93B13-93C1
          Sequence location
          FlyBase Computed Cytological Location
          Cytogenetic map
          Evidence for location
          93B13-93C1
          Limits computationally determined from genome sequence between P{PZ}Atpα01164 and P{PZ}mod(mdg4)07038&P{lacW}mod(mdg4)L3101
          Experimentally Determined Cytological Location
          Cytogenetic map
          Notes
          References
          93B-93C
          (Miletich, 2000.1.10)
          93B10-93B11
          (Jiang, 1997.11.3)
          93B10-93B11
          (determined by in situ hybridisation)
          (Spradling et al., 1999)
          93B1-93B11
          (determined by in situ hybridisation)
          (Jiang and Struhl, 1998)
          62B4-62B5
          68C12--13 93B10--11
          (BDGP Project Members, 1994-1999)
          Experimentally Determined Recombination Data
          Location

          3-70

          (FlyBase, 2016)

          3-73.7

          (Comeron et al., 2012)

          3-

          (Menut et al., 2007, Martin et al., 2003)
          Left of (cM)
          Right of (cM)
          Notes

          Maps to 3R.

          (Menut et al., 2007, Martin et al., 2003)
          Stocks and Reagents
          Stocks (25)
          Genomic Clones (22)
          cDNA Clones (124)
           

          Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

          cDNA clones, fully sequenced
          BDGP DGC clones
          Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           

          polyclonal

          (Brownlee et al., 2011)
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of

          Source for identity of: slmb CG3412

          (Gong, 1999.11)
          Source for database merge of

          Source for merge of: slmb CG3412

          (FlyBase, 1996-)

          Source for merge of: slmb wel

          (Morais-de-Sá et al., 2014)

          Source for merge of: slmb BcDNA:GM02031

          (FlyBase, 1996-)

          Source for merge of: slmb shv

          (Theodosiou et al., 1998)

          Source for merge of: slmb ica

          (Limbourg-Bouchon and Miletich, 1999)

          Source for merge of: slmb MENE(3R)-B

          (Skwarek et al., 2014)
          Additional comments

          Source for merge of slmb CG3412 was sequence comparison ( date:000414 ).

          (FlyBase, 1996-)

          Source for merge of slmb BcDNA:GM02031 was a shared cDNA ( date:030728 ).

          (FlyBase, 1996-)
          Other Comments

          slmb antagonises the aPKC/par-6 complex to control oocyte and epithelial polarity.

          (Morais-de-Sá et al., 2014)

          slmb maintains an S-phase reduplication block by down-regulating SAK on centrioles.

          (Rogers et al., 2009)

          Processing of ci-155 to the repressor ci-75 is mediated by direct binding to slmb.

          (Smelkinson and Kalderon, 2006)

          Identified as a component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells.

          (Nybakken et al., 2005)

          dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

          (Kiger et al., 2003)

          Identified in a germline clone screen for mutants that are defective in localisation of an Avic\GFP-stau marker in living oocytes. 2 alleles of wel have been identified in the screen.

          (Martin et al., 2003)

          slmb is an essential part of the circadian clock, it controls the levels of clock proteins per and tim.

          (Grima et al., 2002)

          slmb is likely to be involved, in addition to its repression role on dpp and wg, in some other essential cellular mechanism that would be essential for maintaining imaginal cells affinities.

          (Miletich and Limbourg-Bouchon, 2000)

          Isolated in an EMS screen for recessive mutations that alter normal adult patterning in somatic clones.

          (Jiang and Struhl, 1998)

          Loss of slmb function results in a cell-autonomous accumulation of high levels of both ci and arm and ectopic expression of both hh- and wg- responsive genes.

          (Jiang and Struhl, 1998)

          slmb negatively regulates hh and wg signal transduction.

          (Jiang and Struhl, 1998)

          Strong alleles cause embryonic lethality while weak alleles cause larval and pupal lethality.

          (Theodosiou et al., 1998)

          slmb coordinates wg and dpp expression in the dorsal-ventral and anterior-posterior axes during limb development.

          (Theodosiou et al., 1998)

          Isolated in a screen for mutations that modify fu phenotypes.

          (Miletich and Limbourg-Bouchon, 1997)

          Identified in an adult mosaic screen for negative regulators of cell proliferation.

          (Theodosiou et al., 1996)
          Origin and Etymology
          Discoverer
          Etymology

          Given the name "pingiel", after one of Pavlov's dogs.

          (Dubnau et al., 2003)

          To reflect the fact that many of the mutants isolated in this screen disrupt Avic\GFP-stau localisation to the anterior and/or posterior pole of the oocyte, many of the complementation groups identified in the screen have been named after polar explorers who failed to reach the North or South pole.

          (Martin et al., 2003)
          Identification
          External Crossreferences and Linkouts ( 80 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          Linkouts
          Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
          KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
          MIST (genetic) - An integrated Molecular Interaction Database
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Synonyms and Secondary IDs (30)
          Reported As
          Symbol Synonym
          BcDNA:GM02031
          CG3412
          (Erez et al., 2021, Çiçek et al., 2016, Hosono et al., 2015, Roberts et al., 2012, Kirienko and Fay, 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Ni et al., 2008, Nybakken et al., 2005, Das et al., 2002)
          FBXW1
          (Roberts et al., 2012)
          MENE (3R)-B
          (Menut et al., 2007)
          MENE(3R)-B
          (Skwarek et al., 2014)
          SLIMB
          (Patke et al., 2020, He et al., 2017, Mendoza-Viveros et al., 2017, Merbitz-Zahradnik and Wolf, 2015, Tataroglu and Emery, 2015, Allada and Chung, 2010)
          SLMB
          (Srikanta and Cermakian, 2021, Du et al., 2018, Rosin et al., 2018, Dubowy and Sehgal, 2017, Jouette et al., 2017, Ingham et al., 2011, Bajpe et al., 2008)
          Slimb
          (Tokamov et al., 2021, Kang et al., 2020, Li et al., 2020, Rowley et al., 2019, Su, 2019, Boese et al., 2018, Lin et al., 2018, Pan et al., 2017, Richardson and Portela, 2017, Bandyopadhyay et al., 2016, Lee et al., 2016, Khire et al., 2015, Klebba et al., 2015, Zhou et al., 2015, Chen and Jiang, 2013, Wong et al., 2013, Yu and Guan, 2013, Roberts et al., 2012, Brownlee et al., 2011, Godin et al., 2010, Cunha-Ferreira et al., 2009, Djagaeva and Doronkin, 2009, Rogers et al., 2009, Skaar et al., 2009, Huangfu and Anderson, 2006, Jia and Jiang, 2006, Jiang, 2006, Smelkinson and Kalderon, 2006, Jia et al., 2005, Torroja et al., 2005, Park et al., 2004, Reynolds et al., 2004, Jiang, 2002, Ko et al., 2002, Wojcik and Hays, 2000, Aza-Blanc and Kornberg, 1999, Morin, 1999)
          Slimb/beta-TrCP
          (Brownlee and Rogers, 2013)
          Slimb/Β-TRCP1
          (Smelkinson and Kalderon, 2006)
          Slimb/β-TRCP
          (Jia et al., 2005)
          Slimb/βTrCP
          (Cunha-Ferreira et al., 2013)
          Slmb
          (Zhang et al., 2020, AlHaj Abed et al., 2019, Chen et al., 2019, Fulford et al., 2018, Gray et al., 2018, Senaratne et al., 2016, Nagarkar-Jaiswal et al., 2015, Brauchle et al., 2014, Caussinus et al., 2011, Shibutani et al., 2008, Silva et al., 2007)
          crd
          ica
          (Miletich and Limbourg-Bouchon, 1998, Miletich and Limbourg-Bouchon, 1997)
          l(3)00295
          shv
          (Theodosiou et al., 1998, Theodosiou et al., 1996)
          slimb
          (Wang et al., 2019, Zouaz et al., 2018, Chen et al., 2017, Zhang et al., 2016, Colin et al., 2014, Buster et al., 2013, Fan et al., 2013, Chen and Verheyen, 2012, Marchal et al., 2012, Roberts et al., 2012, Chiu et al., 2011, Zhou and Kalderon, 2011, Dubruille et al., 2009, Ho et al., 2009, Bajpe et al., 2008, Kim et al., 2007, Ho et al., 2006, Price, 2006, Nybakken et al., 2005, Muzzopappa and Wappner, 2004, Ogden et al., 2004, Sherr, 2004, Wang and Jiang, 2004, Donaldson et al., 2003, Langloh et al., 2003, Lee et al., 2003, Ou et al., 2003, Jin and Harper, 2002, Khush et al., 2002, Meraldi and Nigg, 2002, Netter et al., 2002, Noureddine et al., 2002, Noureddine et al., 2002, Ou et al., 2002, Rouyer et al., 2002, DeSalle and Pagano, 2001, Lee and Treisman, 2001, Potter and Xu, 2001, Silverman and Maniatis, 2001, Methot and Basler, 2000, Miletich and Limbourg-Bouchon, 2000, Seidensticker and Behrens, 2000, Wojcik et al., 2000, Bienz, 1999, Dong et al., 1999, Maniatis, 1999, Novak and Dedhar, 1999, Polakis, 1999, Spencer et al., 1999, Wang et al., 1999, Johnston and Scott, 1998, Ohlmeyer and Kalderon, 1998, Patton et al., 1998, Jiang, 1997.11.3)
          slmb
          (Barbosa et al., 2021, Evans et al., 2021, Parkhitko et al., 2021, Aboukilila et al., 2020, Wang et al., 2020, Fulford et al., 2019, Meltzer et al., 2019, Rosin et al., 2019, Collu et al., 2018, Li et al., 2017, Gene Disruption Project members, 2016-, Mao et al., 2016, Ricolo et al., 2016, Zhang et al., 2015, Colin et al., 2014, Huelsmann, 2014.12.18, Kerr et al., 2014, Li et al., 2014, Morais-de-Sá et al., 2014, Ribeiro et al., 2014, Skwarek et al., 2014, Yan et al., 2014, Christiansen et al., 2013, Das et al., 2013, Fereres et al., 2013, Kwon et al., 2013, Kwon et al., 2013, McPhee et al., 2013, Morais-de-Sá et al., 2013, Spratford and Kumar, 2013, Zhang et al., 2013, Grima et al., 2012, Hazelett et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Joyce et al., 2012, Lee et al., 2012, Abdou et al., 2011, Du et al., 2011, Jungreis et al., 2011, Neumüller et al., 2011, Rohn et al., 2011, Toku et al., 2011, Arancio et al., 2010, Pospisilik et al., 2010, Djagaeva and Doronkin, 2009, Berger et al., 2008, Christensen et al., 2008.4.15, DasGupta et al., 2007, Kuo et al., 2006, DasGupta et al., 2005, Stanyon et al., 2004, Heriche et al., 2003)
          wel
          (Morais-de-Sá et al., 2014)
          β-TrCP
          (van Soldt and Cardoso, 2020, Tsai and Galko, 2019)
          βTrCP
          (Bandyopadhyay et al., 2016)
          Name Synonyms
          SCF-βTrCP complex
          (Marthiens and Basto, 2020)
          SLIMB
          (Xiao et al., 2015, Chiu et al., 2008)
          Slimb
          (Harrison et al., 2020, Hu et al., 2017, Nguyen et al., 2015, Cunha-Ferreira et al., 2013, Zhang et al., 2013, Zhang et al., 2013, Gonsalves et al., 2011, Peschel and Helfrich-Förster, 2011, Dzhindzhev et al., 2010, Godin et al., 2010, Alexandre et al., 2008, Sathyanarayanan et al., 2008, Wang and Price, 2008, Smelkinson et al., 2007)
          Supernumerary limbs
          (Ho et al., 2009)
          centrosome replication defective
          icare
          (Miletich and Limbourg-Bouchon, 1997)
          pingiel
          (Berger et al., 2008, Dubnau et al., 2003)
          shiva
          (Theodosiou et al., 1998, Theodosiou et al., 1998, Theodosiou et al., 1996)
          slimb
          (Doerflinger et al., 2022, Li et al., 2020, Xi et al., 2017, Morais-de-Sá et al., 2013, Spratford and Kumar, 2013, Marthiens et al., 2012, Du et al., 2011, Zhou and Kalderon, 2011, Rogers et al., 2009, Ho et al., 2008, Takacs et al., 2008, Mathieu et al., 2007, Van Gelder, 2006, Ishii, 2005, Leulier et al., 2003)
          supernumerary limbs
          (Li et al., 2014, Hazelett et al., 2012, Abdou et al., 2011, DasGupta et al., 2007, Ho et al., 2006, Beckstead et al., 2005)
          wellman
          (Morais-de-Sá et al., 2014, Martin et al., 2003)
          Secondary FlyBase IDs
          • FBgn0260565
          • FBgn0267841
          • FBgn0023423
          • FBgn0016060
          • FBgn0023437
          • FBgn0063206
          • FBgn0067639
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (0)
          Result
          Result Type
          Title
          References (339)