FB2022_03, released June 9, 2022

Gene: Dmel\Vps4

Open Close
General Information
Symbol
Dmel\Vps4
Species
D. melanogaster
Name
Vacuolar protein sorting 4
Annotation Symbol
CG6842
Feature Type
protein_coding_gene
FlyBase ID
FBgn0283469
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
Vacuolar protein sorting 4 (Vps4) encodes an AAA family ATPase. It catalyzes the ATP-dependent dissociation of class E VPS proteins from endosomal membranes. It functions in endosomal sorting of cargo into the multivesicular body for lysosomal degradation, abscission during cell division, virus release and nuclear envelope membrane sealing after cell division. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)
All Summaries
Also Known As

BcDNA:GH02678 , dvps4

Key Links
Genomic Location
Cytogenetic map
16F1-16F2
Sequence location
Recombination map
1-59
RefSeq locus
NC_004354 REGION:17907692..17911097
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (15 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables ATP hydrolysis activity
inferred from sequence or structural similarity with UniProtKB:O75351
(FlyBase Curators, 2008-)
inferred from sequence or structural similarity with SGD:S000006377
(Rodahl et al., 2009)
Biological Process (11 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
involved_in actin cytoskeleton organization
inferred from mutant phenotype
(Rodahl et al., 2009)
involved_in autophagosome maturation
inferred from mutant phenotype
(Rusten et al., 2007)
involved_in establishment or maintenance of epithelial cell apical/basal polarity
inferred from mutant phenotype
(Rodahl et al., 2009)
involved_in neuron remodeling
inferred from mutant phenotype
(Loncle et al., 2015)
involved_in positive regulation of epidermal growth factor receptor signaling pathway
inferred from mutant phenotype
(Legent et al., 2015)
inferred from genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205
(Legent et al., 2015)
inferred from genetic interaction with FLYBASE:Egfr; FB:FBgn0003731
(Legent et al., 2015)
inferred from genetic interaction with FLYBASE:rho; FB:FBgn0004635
(Legent et al., 2015)
inferred from genetic interaction with FLYBASE:spi; FB:FBgn0005672
(Legent et al., 2015)
involved_in R8 cell development
inferred from mutant phenotype
(Legent et al., 2015)
involved_in R8 cell-mediated photoreceptor organization
inferred from mutant phenotype
(Legent et al., 2015)
involved_in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
inferred from mutant phenotype
(Rodahl et al., 2009)
involved_in viral entry into host cell
inferred from high throughput mutant phenotype
(Hodgson et al., 2019)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in endosomal transport
inferred from biological aspect of ancestor with PANTHER:PTN000553912
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in vacuole organization
inferred from biological aspect of ancestor with PANTHER:PTN000553912
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in endosome
inferred from sequence or structural similarity with UniProtKB:P52917
(Rodahl et al., 2009)
part_of ESCRT IV complex
inferred from sequence or structural similarity with UniProtKB:P52917
(FlyBase Curators, 2008-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Vacuolar protein sorting 4 (Vps4) encodes an AAA family ATPase. It catalyzes the ATP-dependent dissociation of class E VPS proteins from endosomal membranes. It functions in endosomal sorting of cargo into the multivesicular body for lysosomal degradation, abscission during cell division, virus release and nuclear envelope membrane sealing after cell division. [Date last reviewed: 2019-03-21]
Gene Group (FlyBase)
VPS4-VTA1 COMPLEX -
The ESCRT (Endosomal Sorting Complexes Required for Transport) machinery is made up of five complexes, ESCRT-0 to -III and the Vps4-Vta1 ATPase complex, which are sequentially recruited to the cytosolic face of the membrane, and mediate cargo recognition, membrane budding and fission. The Vps4-Vta1 complex catalyzes disassembly of the ESCRT-III filament around the neck of the budding vesicle in an ATP-driven reaction, resulting in membrane scission and recycling of the ESCRT-III components back to the cytosol. (Adapted from PMID:22361144 and FBrf0209668).
OTHER ATPASES -
The 'other ATPases group comprises ATPases that do not fit into any of the other major ATPase groups.
Pathway (FlyBase)
Positive Regulators of EGFR Signaling Pathway -
Positive regulators of Epidermal Growth Factor Receptor signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl) or acting on downstream effectors.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Vps4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9Y162)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.50

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene
gene
(Legent et al., 2012)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Vps4-RA
FBtr0074504
NM_133030
2031
442
Vps4-RB
FBtr0342787
NM_001298467
2411
442
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Vps4-PA
FBpp0074278
49.6
442
6.96
Q9Y162
NP_573258
AAF48783
Vps4-PB
FBpp0309628
49.6
442
6.96
Q9Y162
NP_001285396
AHN59866
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

442 aa isoforms: Vps4-PA, Vps4-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Vps4 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:
 low
high 
Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.
Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:
 low
high 
as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Rodahl et al., 2009)
third instar larval stage
(Rodahl et al., 2009)
adult stage | male
(Rodahl et al., 2009)
adult stage | female
(Rodahl et al., 2009)
ovary
(Rodahl et al., 2009)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Vps4 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Vps4
Transgenic constructs containing regulatory region of Vps4
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(1)Vps4Δ7b
(Rodahl et al., 2009)
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
cell lethal, with Scer\GAL4unspecified
lethal, with Scer\GAL4Mef2.PR
lethal, with Scer\GAL4pnr-MD237
lethal | pupal stage, with Scer\GAL4pnr-MD237
lethal - all die before end of pupal stage, with Scer\GAL4Bx-MS1096-KE
partially lethal, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
partially lethal, with Scer\GAL4tin.CΔ4
Sterility
Allele
female semi-sterile, with Scer\GAL4nos.PG
female sterile, with Scer\GAL4nos.PG
Other Phenotypes
Allele
abnormal developmental rate | larval stage, with Scer\GAL4Bx-MS1096-KE
abnormal neuroanatomy | P-stage, with Scer\GAL4ppk.PU
abnormal neuroanatomy | P-stage | RU486 conditional, with Scer\GAL4GSG2295
abnormal neuroanatomy | pupal stage, with Scer\GAL4ppk.1.9
abnormal size | prepupal stage P1, with Scer\GAL4ppk.PU
hyperactive | adult stage, with Scer\GAL4elav.PU
hypoactive | adult stage, with Scer\GAL4elav.PU
increased cell death, with Scer\GAL4Bx-MS1096-KE
increased cell death | somatic clone | third instar larval stage
some die during pupal stage, with Scer\GAL4Bx-MS1096-KE
visible, with Scer\GAL4Bx-MS1096
visible | somatic clone
Phenotype manifest in
Allele
abdominal dorsal multidendritic neuron | P-stage, with Scer\GAL4221
actin filament & dorsal mesothoracic disc, with Scer\GAL4Bx-MS1096-KE
actin filament & egg chamber, with Scer\GAL4hs.PB
dendrite | pupal stage, with Scer\GAL4ppk.1.9
dendritic tree | P-stage | RU486 conditional, with Scer\GAL4GSG2295
dendritic tree | P-stage | somatic clone, with Scer\GAL4ppk.PU
embryonic/larval fat body, with Scer\GAL4Cg.PA
endosome | prepupal stage P1, with Scer\GAL4ppk.PU
endosome | P-stage, with Scer\GAL4ppk.PU
endosome | P-stage | RU486 conditional, with Scer\GAL4GSG2295
eye | somatic clone
eye disc | somatic clone | third instar larval stage
larval dorsal multidendritic neuron ddaC | prepupal stage P1, with Scer\GAL4ppk.PU
larval dorsal multidendritic neuron ddaC | P-stage, with Scer\GAL4ppk.PU
larval dorsal multidendritic neuron ddaC | P-stage | RU486 conditional, with Scer\GAL4GSG2295
larval dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4ppk.1.9
multivesicular body | third instar larval stage, with Scer\GAL4en.PU
ommatidium | somatic clone
photoreceptor cell R1 | somatic clone | third instar larval stage
photoreceptor cell R2 | somatic clone | third instar larval stage
photoreceptor cell R3 | somatic clone | third instar larval stage
photoreceptor cell R4 | somatic clone | third instar larval stage
photoreceptor cell R5 | somatic clone | third instar larval stage
photoreceptor cell R6 | somatic clone | third instar larval stage
photoreceptor cell R7 | somatic clone | third instar larval stage
photoreceptor cell R8 | somatic clone | third instar larval stage
sarcomere, with Scer\GAL4Mef2.PR
vesicle | third instar larval stage, with Scer\GAL4en.PU
wing, with Scer\GAL4Bx-MS1096
wing disc, with Scer\GAL4Bx-MS1096-KE
wing disc | somatic clone | third instar larval stage
wing disc posterior compartment, with Scer\GAL4en.PU
Z disc, with Scer\GAL4Mef2.PR
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\VPS4A
13 of 15
Yes
Yes
Hsap\VPS4B
13 of 15
Yes
Yes
Hsap\PIK3R2
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Vps4a
13 of 15
Yes
Yes
Mmus\Vps4b
12 of 15
No
Yes
Mmus\Katnal2
1 of 15
No
No
Rattus norvegicus (Norway rat) (3)
Rnor\Vps4a
12 of 13
Yes
Yes
Rnor\Vps4b
11 of 13
No
Yes
Rnor\Katnal2
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (3)
Xtro\vps4a
12 of 12
Yes
Yes
Xtro\vps4b
7 of 12
No
Yes
Xtro\katnal2
1 of 12
No
No
Danio rerio (Zebrafish) (3)
Drer\vps4b
11 of 15
Yes
Yes
Drer\vps4a
8 of 15
No
Yes
Drer\katnal2
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\vps-4
13 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (3)
Atha\SKD1
8 of 9
Yes
Yes
Atha\AT1G08270
1 of 9
No
Yes
Atha\CCP1
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\VPS4
13 of 15
Yes
Yes
Scer\SAP1
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\vps4
10 of 12
Yes
Yes
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (7)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG10793
3 of 10
Fign
2 of 10
Kat60
2 of 10
kat-60L1
2 of 10
spas
2 of 10
CG4701
1 of 10
nmd
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Vps4HM04061
model of  attention deficit hyperactivity disorder
CEA
(Jia et al., 2016)
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
VPS4A; vacuolar protein sorting 4 homolog A
13 of 15
609982
    VPS4B; vacuolar protein sorting 4 homolog B
    13 of 15
    609983
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Vps4 - CCT3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - CCT8
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - CG33303
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - CG5854
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - CHMP2B
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Chmp1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Cnx99A
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Cyp12d1-p
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Gfat2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Glt
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - GluProRS
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Idh3a
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Ist1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Kap-α3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Mcad
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Mpcp2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Pdk1
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Vps4 - Pi3K68D
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Pp2A-29B
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Rdh1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - ScsβG
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Sec61α
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Sec63
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - SerRS
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Tapδ
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Vps2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Vps24
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - Vps60
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - chico
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - mib2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - ninaA
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - r
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - shrb
        experimental knowledge based
        (Guruharsha et al., 2011)
        Vps4 - wal
        experimental knowledge based
        (Guruharsha et al., 2011)
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Vps4
        suppressible
        bsk
        (Rodahl et al., 2009)
        Vps4
        suppressible
        Dronc
        (Legent et al., 2015)
        Vps4
        enhanceable
        Nrg
        (Zhang et al., 2014)
        Vps4
        suppressible
        Nrg
        (Zhang et al., 2014)
        Vps4
        suppressible
        puc
        (Rodahl et al., 2009)
        Vps4
        suppressible
        Ras85D
        (Legent et al., 2015)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Ras85D
        suppressible
        Vps4
        (Friedman et al., 2011)
        shi
        suppressible
        Vps4
        (Legent et al., 2015)
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Positive Regulators of EGFR Signaling Pathway -
        Positive regulators of Epidermal Growth Factor Receptor signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl) or acting on downstream effectors.
        Metabolic Pathways
        External Data
        Linkouts
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        X
        Recombination map
        1-59
        Cytogenetic map
        16F1-16F2
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        16F1-16F2
        Limits computationally determined from genome sequence between P{EP}EP1464 and P{EP}EP970
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        1-59

        (FlyBase, 2016)

        1-58.9

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (7)
        Genomic Clones (4)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (46)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Rodahl et al., 2009)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: Vps4 CG6842

        (Rodahl et al., 2009)
        Source for database merge of

        Source for merge of: Vps4 3B1

        (Legent et al., 2015)
        Additional comments
        Other Comments

        Vps4 is required cell autonomously for dendrite pruning in sensory neurons.

        (Loncle et al., 2015)

        S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

        (Stroschein-Stevenson et al., 2006)

        dsRNA made from templates generated with primers directed against CG6842 that is transfected into S2 treated with Listeria monocytogenes reveals CG6842 to be involved in Listeria monocytogenes entry, intracellular growth and vacuolar escape.

        (Cheng et al., 2005)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 39 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (12)
        Reported As
        Symbol Synonym
        3B1
        BcDNA:GH02678
        (Giot et al., 2003)
        CG6842
        (Widmer et al., 2018, Hosono et al., 2015, Gupta et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Lindquist et al., 2011, Hilger et al., 2009, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Rodahl et al., 2009, Ni et al., 2008, Rusten et al., 2007, Stroschein-Stevenson et al., 2006)
        DmBcDNA.GH02678
        (Frohlich, 2001)
        Dvps4
        (Gupta et al., 2014)
        Vps4
        (Pennemann et al., 2021, Reiff et al., 2021, Gohel et al., 2020, González-Méndez et al., 2020, McGough et al., 2020, Rui et al., 2020, Hodgson et al., 2019, Khezri and Rusten, 2019, Meltzer et al., 2019, Wang et al., 2019, Widmer et al., 2018, Zong et al., 2018, Beer and Wehman, 2017, Harden, 2017, Martinez et al., 2017, Jia et al., 2016, Malartre, 2016, Dent et al., 2015, Legent et al., 2015, Li and Blissard, 2015, Loncle et al., 2015, Parchure et al., 2015, Sopko et al., 2015, Gradilla et al., 2014, Zhang et al., 2014, Czech et al., 2013, Kwon et al., 2013, Friedman et al., 2011, Michelet et al., 2010, Wasbrough et al., 2010, Philips et al., 2008, Rodahl et al., 2007, Rusten et al., 2007, Rusten et al., 2007)
        Vps4B
        (Lindquist et al., 2011)
        bcDNA:GH02678
        (Stuart et al., 2007)
        dvps4
        (Gupta et al., 2014, Rodahl et al., 2009)
        vps4
        (Gore et al., 2021, Baeumers et al., 2020, González-Méndez et al., 2020, Bäumers et al., 2019, Troost et al., 2012, Kammermeier et al., 2003)
        Name Synonyms
        CG6842
        Vacuolar protein sorting 4
        (Malartre, 2016)
        vacuolar protein sorting 4
        (Rusten et al., 2007)
        Secondary FlyBase IDs
        • FBgn0264354
        • FBgn0027605
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
        Title
        References (91)