dS6K, S6 kinase, p70S6K, fs(3)07084, RPS6-p70-protein kinase
signaling - regulates growth response - targets ribosomal protein S6 - a target of the TOR pathway - essential for Myc-dependent rDNA transcription
Please see the JBrowse view of Dmel\S6k for information on other features
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Gene model reviewed during 5.45
Gene model reviewed during 5.43
Gene model reviewed during 5.46
Low-frequency RNA-Seq exon junction(s) not annotated.
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
5.0, 3.7, 2.8 (northern blot)
5.0, 3.7 (northern blot)
5.0, 3.0, 2.5, 2.3 (northern blot)
490 (aa)
637 (aa); 73 (kD predicted)
637 (aa); 76 (kD predicted)
Two peaks of S6 kinase activity were observed in
S2 cells, one of which is rapamycin sensitive. The cloned S6k was shown
to have identity with the rapamycin sensitive form.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\S6k using the Feature Mapper tool.
S6k protein is detected in extracts of S2 cells.
Comment: expression throughout adult brain
GBrowse - Visual display of RNA-Seq signals
View Dmel\S6k in GBrowse 23-15
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Source for identity of: S6k CG10539
Source for merge of: S6k Pk64F
BLAST of sequence given in Fig 9 of FBrf0048100 together with cytological location indicates that 'Pk64F' from FBrf0048100 corresponds to 'S6k'.
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
dsRNA made from templates generated with primers directed against this gene used to treat S2 cells.
dsRNA made from templates generated with primers directed against this gene increases Akt1 phosphorylation in S2 cells.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G1 phase cells is seen.
Promotes starvation-induced autophagy in the fat body.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: increased or polarized (uneven) accumulation of F-actin.
Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.
The S6k gene product regulates cell size in a cell-autonomous manner without impinging on cell number.
Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. S6k is required for germ cell viability or early oogenesis.
Isolated using a rat p70s6k fragment as a probe.
S6k has been cloned and sequenced.
Isolated from a cDNA library using rat p70S6k cDNA sequences as a probe, under low stringency conditions.
Isolated from a genomic library using a Pka-C1 probe.
Pk64F may encode a protein kinase.
Gene in D.melanogaster encoding product related (by sequence comparison) to the serine-threonine protein kinases of mammals. Isolated from Drosophila clones obtained with mammalian probes.
