lig3, DNA ligase III
Gene model reviewed during 5.53
Gene model reviewed during 5.45
Low-frequency RNA-Seq exon junction(s) not annotated.
There is only one protein coding transcript and one polypeptide associated with this gene
The properties of two DNA ligases purified from
embryos were compared. The enzymes differ in size, thermostability,
affinity for ATP, Km, and optimal reaction conditions (salt optima,
requirement for DTT, and reaction to PVA). They are similar in requirement
for ATP as cofactor and Mg2+ as divalent cation, inhibition by
N-ethylmaleimide, optimal ligation temp, and substrate specificity. The
major AMP-adduct has a molecular weight of 70kD.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\DNAlig3 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\DNAlig3 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: lig3 DNA-ligII
'DNA-ligII' (FBgn0013775) was an unannotated (non-CG) gene, created based on an enzymatic activity described by Takahashi and Senshu, 1987 (FBrf0046241) and Takahashi and Tomizawa, 1990 (FBrf0051704). The described characteristics of 'DNA-ligII' included: i) a relatively low activity throughout all developmental stages; ii) nuclear concentration; iii) thought to be similar to mammalian DNA ligase II. Of the two possible annotated candidates (DNA ligases 3 and 4), DNA ligase 3 is the best match - it's expressed at a low level throughout development, it's predicted to be nuclear, and it's the ortholog of mammalian LIG3 (aka LIG2). On this basis, 'DNA-ligII' was merged into the 'DNAlig3' report.
Through BLAST similarity (51%) to human LIG3.