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General Information
Symbol
Dmel\Vha44
Species
D. melanogaster
Name
Vacuolar H+ ATPase 44kD subunit
Annotation Symbol
CG8048
Feature Type
FlyBase ID
FBgn0287825
Gene Model Status
Stock Availability
Gene Summary
Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. In enterocytes, acts as part of a pHCl-2 sensory pathway which mediates Tor-dependent larval growth and metabolism in response to zinc availability (PubMed:32269334). Likely acts in maintaining enterocyte lysosomal acidification which consequently promotes Tor activation at the lysosome membrane (PubMed:32269334). (UniProt, Q9V7N5)
Contribute a Gene Snapshot for this gene.
Also Known As

Vacuolar H+ ATPase 44kD C subunit, Vacuolar H+ ATPase 44kD C subunit, l(2)02836

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
2-79
RefSeq locus
NT_033778 REGION:16324813..16336637
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR004907, InterPro:IPR036132
(assigned by InterPro )
inferred from sequence or structural similarity
inferred by curator from GO:0033181
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with SGD:S000001563
inferred from sequence or structural similarity
Protein Family (UniProt)
Belongs to the V-ATPase C subunit family. (Q9V7N5)
Summaries
Gene Group (FlyBase)
VACUOLAR ATPASE V1 DOMAIN NON-CATALYTIC SUBUNITS -
The V1 domain of V-ATPases is a peripheral membrane domain composed of eight different subunits (A-H) that are expressed as multiple isoforms in a tissue-specific manner. The V1 domain is responsible for ATP hydrolysis. The non-catalytic V1 subunits do not hydrolyze ATP directly but provide regulatory functions and/or interact with the V0 domain. (Adapted from PMID:20450191 and FBrf0188241).
Protein Function (UniProtKB)
Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. In enterocytes, acts as part of a pHCl-2 sensory pathway which mediates Tor-dependent larval growth and metabolism in response to zinc availability (PubMed:32269334). Likely acts in maintaining enterocyte lysosomal acidification which consequently promotes Tor activation at the lysosome membrane (PubMed:32269334).
(UniProt, Q9V7N5)
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\Vha44 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0087173
1470
388
FBtr0087176
1431
388
FBtr0087175
2693
442
FBtr0290031
3875
836
FBtr0290032
3820
836
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0086317
44.5
388
7.96
FBpp0086320
44.5
388
7.96
FBpp0086319
51.2
442
8.94
FBpp0288470
92.4
836
7.59
FBpp0288471
92.4
836
7.59
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

836 aa isoforms: Vha44-PE, Vha44-PF
388 aa isoforms: Vha44-PA, Vha44-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d).

(UniProt, Q9V7N5)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Vha44 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Vha44 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 17 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Vha44
Transgenic constructs containing regulatory region of Vha44
Aberrations (Deficiencies and Duplications) ( 4 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
10 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
9 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
12 of 13
Yes
Yes
8 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
13 of 12
Yes
Yes
10 of 12
No
Yes
Danio rerio (Zebrafish) (3)
14 of 15
Yes
Yes
13 of 15
No
Yes
7 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
14 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
9 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
13 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
10 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091904FT )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502I0 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02A5 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X027L )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G03CP )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 2 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d).
(UniProt, Q9V7N5 )
Linkouts
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-79
Cytogenetic map
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
53B1-53C1
Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338); Limits computationally determined from genome sequence between P{lacW}Lis-1k11702&P{EP}CG8443EP969 and P{lacW}l(2)k07824k07824&P{lacW}vegk03402
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Complementation tests with molecularly defined deletions place the P{PZ}l(2)02836a02836a insertion in the interval 2R:16187888--16386515 (release 6 genome), equivalent to a cytological location of 52F6--53C4.
53B3-53B4
53B1-53B2
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (18)
Genomic Clones (20)
cDNA Clones (128)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of

    Source for merge of: Vha44 l(2)SH1339

    Source for merge of: Vha44 l(2)02836a

    Additional comments

    l(2)02836a may correspond to Vha44: the P{lacW}l(2)02836ak05440 insertion maps within the transcription unit.

    Other Comments

    dsRNA made from templates generated with primers directed against Vha44 that is transfected into S2 treated with Listeria monocytogenes reveals Vha44 to be involved in Listeria monocytogenes entry and vacuolar escape.

    22 alleles of l(2)SH1339 recovered in a P-insertion screen.

    Area matching Drosophila EST AA699128. This EST matches the 5' of drosophila V-ATP ase gene AF006655 gene.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 48 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    InterPro - A database of protein families, domains and functional sites
    MARRVEL_MODEL - MARRVEL (model organism gene)
    Synonyms and Secondary IDs (20)
    Reported As
    Symbol Synonym
    l(2)02836a
    l(2)SH1339
    l(2)SH2 1339
    Name Synonyms
    C subunit
    V-ATPase C subunit
    Vacuolar H+ ATPase 44kD C subunit
    Vacuolar H+ ATPase 44 kDa subunit
    Vacuolar H+ ATPase 44kD C subunit
    Vacuolar H+ ATPase 44kD subunit
    lethal (2) 02836a
    lethal (2) SH1339
    vacuolar ATPase C-subunit
    Secondary FlyBase IDs
    • FBgn0020611
    • FBgn0262511
    • FBgn0065466
    • FBgn0010522
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (116)