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General Information
Symbol
Dmel\zip
Species
D. melanogaster
Name
zipper
Annotation Symbol
CG15792
Feature Type
FlyBase ID
FBgn0287873
Gene Model Status
Stock Availability
Gene Summary
Nonmuscle myosin appears to be responsible for cellularization. Required for morphogenesis and cytokinesis (PubMed:24786584). Necessary for auditory transduction: plays a role in Johnston's organ organization by acting in scolopidial apical attachment (PubMed:27331610). Interaction with the myosin ck may be important for this function (PubMed:27331610). (UniProt, Q99323)
Contribute a Gene Snapshot for this gene.
Also Known As

myosin II, MyoII, MHC, myosin heavy chain, Myosin

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:24,990,570..25,011,965 [-]
Recombination map
2-107
RefSeq locus
NT_033778 REGION:24990570..25011965
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (50 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687
inferred from physical interaction with FLYBASE:sqh; FB:FBgn0003514
inferred from physical interaction with UniProtKB:Q9W3N0
(assigned by UniProt )
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR008989
(assigned by InterPro )
inferred from sequence or structural similarity with UniProtKB:P35580
enables ATP binding
inferred from electronic annotation with InterPro:IPR001609, InterPro:IPR004009
(assigned by InterPro )
(assigned by InterPro )
Biological Process (33 terms)
Terms Based on Experimental Evidence (33 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
involved_in head involution
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:fz; FB:FBgn0001085
inferred from genetic interaction with FLYBASE:dsh; FB:FBgn0000499
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
involved_in muscle contraction
inferred from mutant phenotype
involved_in myofibril assembly
inferred from mutant phenotype
inferred from direct assay
inferred from genetic interaction with FLYBASE:kirre; FB:FBgn0028369
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
involved_in spindle assembly
inferred from mutant phenotype
involved_in wound healing
inferred from mutant phenotype
NOT involved_in muscle attachment
inferred from genetic interaction with FLYBASE:l(2)gl; FB:FBgn0002121
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (11 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
inferred from direct assay
colocalizes_with apical cortex
inferred from direct assay
inferred from direct assay
located_in cell cortex
inferred from mutant phenotype
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
located_in cleavage furrow
inferred from direct assay
colocalizes_with contractile ring
inferred from high throughput direct assay
located_in cytosol
inferred from high throughput direct assay
inferred from direct assay
inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687
inferred from physical interaction with FLYBASE:sqh; FB:FBgn0003514
located_in Z disc
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. (Q99323)
Summaries
Gene Group (FlyBase)
MYOSIN MOTORS -
Myosin motors are motor proteins that use the hydrolysis of ATP to drive movement along actin filaments. They can be divided into two groups: conventional myosins (myosin II) are involved in generating the mechanical force for muscle contraction; unconventional myosins (all other classes) are involved in processes such as cell motility, cytokinesis and trafficking. (Adapted from FBrf0134714.)
Protein Function (UniProtKB)
Nonmuscle myosin appears to be responsible for cellularization. Required for morphogenesis and cytokinesis (PubMed:24786584). Necessary for auditory transduction: plays a role in Johnston's organ organization by acting in scolopidial apical attachment (PubMed:27331610). Interaction with the myosin ck may be important for this function (PubMed:27331610).
(UniProt, Q99323)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Mhc-c: Myosin heavy chain-cytoplasmic (D.P. Kiehart)
Encodes a 205 kilodalton myosin heavy chain found in Drosophila cell lines and all Drosophila developmental stages. Antibodies raised against this protein crossreact, but weakly with muscle myosin heavy chain. First appears in preblastoderm embryos; diffusely distributed until syncytial blastoderm at which time localization to cortex and pole cells observed; at cleavage furrow, canals at the time of cellularization; transiently present at points of invagination during gastrulation (Young et al., 1987, 1991).
zip: zipper
The wild-type allele of zipper is expressed in the nervous system during development and was believed to encode an integral membrane protein necessary for normal axon patterning (Zhao et al., 1988); however it has more recently been shown to encode the heavy chain of cytoplasmic myosin (Kierhart). The mutants are embryonic lethals; abnormalities include a small hole in the ventral thorax, distortion of ventral denticle rows, and defects in head involution and dorsal closure (Nusslein-Volhard et al., 1984; Cote et al., 1987). These defects vary in different alleles and in different embryos from the same egg laying (Cote et al., 1987). The nervous system of mutant embryos also differentiates abnormally, showing local defects in the fasciculation pattern of axons (Zhao et al., 1988), as indicated by antibody stains for neurons and their axons. These CNS abnormalities can be detected after germ band shortening and, together with the molecular data, suggested that neurological rather than epidermal defects are the primary ones in zip mutants (Cote et al., 1987).
Gene Model and Products
Number of Transcripts
9
Number of Unique Polypeptides
7

Please see the JBrowse view of Dmel\zip for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0072399
6482
2056
FBtr0072398
6796
2011
FBtr0100466
6298
1971
FBtr0100467
6362
2016
FBtr0302572
6325
1971
FBtr0302573
6322
1979
FBtr0302574
6319
1971
FBtr0302575
6456
1964
FBtr0306576
6386
2024
Additional Transcript Data and Comments
Reported size (kB)

6.455, 6.391, 6.335, 6.271 (sequence analysis)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0072306
236.6
2056
5.30
FBpp0072305
231.5
2011
5.15
FBpp0099894
226.9
1971
5.13
FBpp0099896
232.1
2016
5.27
FBpp0291728
226.9
1971
5.13
FBpp0291729
227.8
1979
5.13
FBpp0291730
226.9
1971
5.13
FBpp0291731
226.8
1964
5.20
FBpp0297531
233.0
2024
5.27
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1971 aa isoforms: zip-PC, zip-PE, zip-PG
Additional Polypeptide Data and Comments
Reported size (kDa)

2057, 2017, 2012, 1972 (aa)

2017, 1972 (aa); 227 (kD predicted)

500 (aa); 56 (kD predicted)

Comments

zip protein is most similar to metazoan smooth and nonmuscle myosins.

A 2017aa zip protein is predicted by translation from the first AUG of the "long" zip transcript. Antibodies directed against a 15aa peptide specific for part of the amino-terminal 45aa extension react with a protein of the expected size providing evidence for its existence in vivo.

External Data
Subunit Structure (UniProtKB)

Interacts with sau (PubMed:24786584). Interacts with ck and Ubr3 (PubMed:27331610).

(UniProt, Q99323)
Post Translational Modification

Ubiquitinated.

(UniProt, Q99323)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\zip using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

radioisotope in situ
Stage
Tissue/Position (including subcellular localization)
Reference
RNase protection, primer extension, SI map
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

zip expression is strong in the amnioserosa and weak in the dorsal epidermis at embryonic stage 11,12 and gone from the amnioserosa and strong in the dorsal epidermis at stage 13.

Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
colocalizes_with apical cortex
inferred from direct assay
inferred from direct assay
located_in cell cortex
inferred from mutant phenotype
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
located_in cleavage furrow
inferred from direct assay
colocalizes_with contractile ring
inferred from high throughput direct assay
located_in cytosol
inferred from high throughput direct assay
inferred from direct assay
inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687
inferred from physical interaction with FLYBASE:sqh; FB:FBgn0003514
located_in Z disc
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\zip in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 35 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 29 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of zip
Transgenic constructs containing regulatory region of zip
Aberrations (Deficiencies and Duplications) ( 8 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
contractile ring & meiotic cell cycle | male
elongation stage spermatid & nucleus
filamentous actin & denticle field primordium
spermatid & nucleus | supernumerary
spindle & meiotic cell cycle | male
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (22)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
11 of 15
No
Yes
1  
10 of 15
No
Yes
0  
9 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
1  
3 of 15
No
No
3 of 15
No
No
0  
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (21)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
11 of 15
No
Yes
10 of 15
No
Yes
8 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (21)
9 of 13
Yes
Yes
9 of 13
Yes
Yes
7 of 13
No
Yes
7 of 13
No
Yes
4 of 13
No
Yes
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
2 of 13
No
No
2 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (29)
6 of 12
Yes
Yes
6 of 12
Yes
Yes
5 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (44)
12 of 15
Yes
Yes
11 of 15
No
Yes
9 of 15
No
Yes
7 of 15
No
Yes
6 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (12)
14 of 15
Yes
Yes
6 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (20)
2 of 9
Yes
No
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (4)
11 of 15
Yes
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (5)
9 of 12
Yes
No
8 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
1 of 12
No
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900CI )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500CC )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00CM )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00BY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G009J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (14)
6 of 10
3 of 10
3 of 10
3 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 6 )
Potential Models Based on Orthology ( 4 )
Modifiers Based on Experimental Evidence ( 4 )
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
suppressible
External Data
Subunit Structure (UniProtKB)
Interacts with sau (PubMed:24786584). Interacts with ck and Ubr3 (PubMed:27331610).
(UniProt, Q99323 )
Linkouts
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-107
Cytogenetic map
Sequence location
2R:24,990,570..25,011,965 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
60E12-60E12
Limits computationally determined from genome sequence between P{EP}CG2790EP412&P{EP}CG3776EP835 and P{EP}zipEP856&P{PZ}l(2)1048103263
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
60F1-60F3
(determined by in situ hybridisation)
60E-60F
(determined by in situ hybridisation)
60E9-60F1
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (26)
Genomic Clones (17)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (157)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of

    Source for merge of: zip cbe

    Source for merge of: zip anon-WO0140519.37

    Source for merge of: zip l(2)17F1

    Additional comments

    Source for merge of zip anon-WO0140519.37 was sequence comparison ( date:051113 ).

    Other Comments

    Host gene for maternally inherited stable intronic sequence RNA (sisRNA).

    Candidate stable intronic sequence RNA (sisRNA) identified within 5'UTR of this gene.

    Overexpression of zip in D.melanogaster males results in paternal-effect lethality that mimics the fertilisation defects associated with cytoplasmic incompatibility (CI) caused by Wolbachia infection.

    dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc167 cells.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

    zip is necessary for normal morphology and behaviour of the leading edge cells during embryonic dorsal closure.

    zip is required for myofibril formation. zip may function at the muscle termini and the Z line as an actin crosslinker and may act to maintain the structural integrity of the sarcomere.

    The zip protein inhibits actomyosin dependent basal protein targeting in neuroblasts.

    158 deficiency chromosomes have been screened for second site non-complementation with zip alleles, identifying at least 39 SSNC loci, including Tm1, vkg and Rho1.

    Mutation rate at microsatellite loci in 119 lines maintained for approximately 250 generations is estimated to be 6.3x10-6, at least one order of magnitude lower than the mutation rate in mammals.

    One of a class of genes with TATA-less promoters that have the conserved DPE sequence.

    zip is required for cell sheet movements in embryos. The zip gene product is responsible for a surprisingly diverse array of cell shape changes throughout development.

    A serine kinase is tightly associated with l(2)gl. Activation of the serine kinase results in the disassociation of zip from the l(2)gl complex without affecting the homo-oligomerisation of l(2)gl.

    Organisation of the transcription unit and alternative splicing of zip are analysed.

    The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to study the zygotic lethal mutation.

    The p127 protein of the l(2)gl gene is a component of a cytoskeletal network when complexed with a nonmuscle myosin II heavy chain protein, zip.

    Severe alleles disrupt cell shape changes required for embryogenesis.

    The zip gene product, nonmuscle myosin, is required for generating and/or maintaining the cell shapes that change during the course of morphogenesis and provides a link between myosin and morphogenesis.

    Analysis of myosin heavy chain antibody staining in Drosophila tissue culture cells and premyogenic embryos suggests that zip rather than Mhc is associated with the nuclear envelope.

    The interaction of zip and br depends on loss of br function and is temperature-dependent. Flies reared at 18oC show a higher penetrance of the mlf phenotype (malformed syndrome), wing malformations and leg defects, than those reared at 25oC.

    zip mutant embryos display defects in dorsal closure, head involution, segmentation and neural pathfinding.

    The distribution of zip protein during embryogenesis has been analysed.

    Fas3, mys, disco, zip, l(2)gl, N and Egfr mutants show an additive phenotype in combination with Fas1TE89Da.

    An alternatively spliced exon at the 5' end of zip generates two distinct transcripts. The coding region reveals extensive homology with other conventional myosins.

    The zip region is defined by the proximal breakpoint of Df(2R)Kr10 (map position 0 to +3.5) and by the proximal breakpoint of Df(2R)gsb (map position -55 to -49).

    Included in genetic and molecular analysis of the zipper-gooseberry region.

    Five structural and three functional criteria demonstrate a protein purified from S2, S3 and Kc culture cells is a cytoplasmic myosin.

    The gene sequenced by Zhao et al. (Zhao et al., 1988, EMBO J. 7) as zip is not zip but the adjacent gene, uzip.

    The mutants are embryonic lethals; abnormalities include a small hole in the ventral thorax, distortion of ventral denticle rows and defects in head involution and dorsal closure (FBrf0041708; FBrf0046110). These defects vary in different alleles and in different embryos from the same egg laying (FBrf0046110).

    Encodes a 205 kilodalton myosin heavy chain found in Drosophila cell lines and all Drosophila developmental stages. Antibodies raised against this protein crossreact, but weakly with muscle myosin heavy chain. First appears in preblastoderm embryos; diffusely distributed until syncytial blastoderm at which time localization to cortex and pole cells observed; at cleavage furrow, canals at the time of cellularization; transiently present at points of invagination during gastrulation (FBrf0046628; FBrf0053751). produces a truncated myosin heavy chain on Western blots and fails to complement zip1 and zip2. Western blots also indicate zip2 fails to accumulate myosin heavy chain.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 62 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Synonyms and Secondary IDs (77)
    Reported As
    Symbol Synonym
    Dronm-MII
    Mhc-c1
    anon-WO0140519.37
    l(2)17F1
    zip
    (Carim et al., 2020, Hung et al., 2020, López-Gay et al., 2020, Santa-Cruz Mateos et al., 2020, West and Harris, 2020, Balaji et al., 2019, Boucher et al., 2019, Chang et al., 2019, Finegan et al., 2019, Hunter et al., 2019, Kasza et al., 2019, Kobb et al., 2019, Park et al., 2019, Proag et al., 2019, Sharma et al., 2019, Thuveson et al., 2019, Córdoba and Estella, 2018, Lebreton et al., 2018, Lee et al., 2018, Mortensen et al., 2018, Nithianandam and Chien, 2018, Park et al., 2018, Tsai et al., 2018, Zhang et al., 2018, Bulgakova et al., 2017, Fochler et al., 2017, Hermle et al., 2017, Ly et al., 2017, Rothenbusch-Fender et al., 2017, Simões et al., 2017, Sun et al., 2017, Tay and Pek, 2017, Transgenic RNAi Project members, 2017-, Fear et al., 2016, Gene Disruption Project members, 2016-, Ng et al., 2016, Rousso et al., 2016, Weng and Wieschaus, 2016, Davis et al., 2015, Gene Disruption Project members, 2015-, Guglielmi et al., 2015, Heissler et al., 2015, Kim et al., 2015, Levayer et al., 2015, Monier et al., 2015, Pek et al., 2015, Sato et al., 2015, Winkler et al., 2015, Ashwal-Fluss et al., 2014, Guo et al., 2014, He et al., 2014, Kitazawa et al., 2014, Merlo et al., 2014, Mukherjee et al., 2014, Nie et al., 2014, Rauskolb et al., 2014, Rudrapatna et al., 2014, Aldaz et al., 2013, Beckett et al., 2013, Bonn et al., 2013, Guthrie, 2013, Külshammer and Uhlirova, 2013, Kwon et al., 2013, Lee and Harris, 2013, Moon and Matsuzaki, 2013, Shen et al., 2013, Taliaferro et al., 2013, Thomae et al., 2013, Abreu-Blanco et al., 2012, Duboff et al., 2012, Gault et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Lesch and Page, 2012, Murray et al., 2012, Rodriguez et al., 2012, Sano et al., 2012, Weber et al., 2012, Axelsson et al., 2011, Escudero et al., 2011, Farkaš et al., 2011, Friedman et al., 2011, Guest et al., 2011, Jiang et al., 2011, Kim et al., 2011, Monier et al., 2011, Neumüller et al., 2011, Rees et al., 2011, Seabrooke and Stewart, 2011, Sun et al., 2011, Baek et al., 2010, Campos et al., 2010, David et al., 2010, Djiane and Mlodzik, 2010, Franke et al., 2010, Gettings et al., 2010, Knowles-Barley et al., 2010, Kwon et al., 2010, Lowery et al., 2010, Monier et al., 2010, Neubueser and Hipfner, 2010, Okumura et al., 2010, Rui et al., 2010, Saja et al., 2010, Simões et al., 2010, Simone and DiNardo, 2010, Aerts et al., 2009, Bertet et al., 2009, Chung et al., 2009, Dworkin et al., 2009, Landsberg et al., 2009, Martin et al., 2009, Patch et al., 2009, Warner and Longmore, 2009, Chen et al., 2008, Christensen et al., 2008.9.3, Fiehler and Wolff, 2008, Homem and Peifer, 2008, Okumura et al., 2008, Pope and Harris, 2008, Rodriguez-Diaz et al., 2008, Todi et al., 2008, Zahedi et al., 2008, Boettner and Van Aelst, 2007, Buszczak et al., 2007, Chung et al., 2007, Dietzl et al., 2007, Dilks and DiNardo, 2007, Escudero et al., 2007, Farkas et al., 2007, Fiehler and Wolff, 2007, Franke et al., 2007, Kaltenbach et al., 2007, Kirchner et al., 2007, Kirchner et al., 2007, Stuart et al., 2007, Brodu and Casanova, 2006, Franke et al., 2006, Hammonds and Fristrom, 2006, Hawley and Gilliland, 2006, Hickson et al., 2006, Verdier et al., 2006, Gim et al., 2001, Winter et al., 2001)
    Name Synonyms
    Myo-II heavy chain
    MyoII heavy chain
    Myosin heavy chain-cytoplasmic
    Nonmuscle MyoII
    Zipper heavy chain
    cytoplasmic myosin
    cytoplasmic myosin heavy chain
    cytoplasmic myosin-II
    lethal (2) 17F1
    myo-II heavy chain
    myosin-heavy-chain-cytoplasmic
    myosins II
    non muscle myosin
    non-muscle myosin
    non-muscle myosin heavy-chain
    non-muscle myosin-II
    nonmuscle Myosin II heavychain
    nonmuscle myosin II heavy chain
    nonmuscle myosin heavy chain
    zipper
    (Cohen et al., 2020, Hunter et al., 2020, Schmidt and Grosshans, 2018, Ng et al., 2016, Weng and Wieschaus, 2016, Wieschaus and Nüsslein-Volhard, 2016, Machado et al., 2015, Sato et al., 2015, Lye et al., 2014, Rudrapatna et al., 2014, Vasquez et al., 2014, Aldaz et al., 2013, Giuliani et al., 2013, Shen et al., 2013, Gault et al., 2012, Lesch and Page, 2012, Petzoldt et al., 2012, Reed et al., 2012, Weber et al., 2012, Bassi et al., 2011, Cammarato et al., 2011, Farkaš et al., 2011, Guest et al., 2011, Laplante and Nilson, 2011, Monier et al., 2011, Seabrooke and Stewart, 2011, Cabernard et al., 2010, Campos et al., 2010, David et al., 2010, Djiane and Mlodzik, 2010, Franke et al., 2010, Gettings et al., 2010, Lowery et al., 2010, Okumura et al., 2010, Ricketson et al., 2010, Simone and DiNardo, 2010, Vielemeyer et al., 2010, Landsberg et al., 2009, Newman and Prehoda, 2009, Pouille et al., 2009, Sawyer et al., 2009, Chen et al., 2008, Liu et al., 2008, Pope and Harris, 2008, Roy et al., 2008, Sokac and Wieschaus, 2008, Todi et al., 2008, Boettner and Van Aelst, 2007, Dilks and DiNardo, 2007, Escudero et al., 2007, Franke et al., 2007, Kaltenbach et al., 2007, Texada et al., 2007, Borghese et al., 2006, Clark et al., 2006, Franke et al., 2006, Hawley and Gilliland, 2006, Hickson et al., 2006, Verdier et al., 2006, Walters et al., 2006, Wang et al., 2006, Dean et al., 2005, Huang et al., 2003, Rogers et al., 2003, Gim et al., 2001, Halsell and Kiehart, 1997, Riesgo-Escovar et al., 1996)
    Secondary FlyBase IDs
    • FBgn0265434
    • FBgn0005634
    • FBgn0002742
    • FBgn0004415
    • FBgn0010524
    • FBgn0044594
    • FBgn0026658
    • FBgn0083095
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (613)