Human Disease Model Report: cancer, neural stem cell, disruption of asymmetric division

General Information

Name

cancer, neural stem cell, disruption of asymmetric division

FlyBase ID

FBhh0000954

Disease Ontology Term

brain cancer

Parent Disease

OMIM

Overview

Asymmetric division is a critical step in the differentiation of post-embryonic stem cells during which each stem cell generates one self-renewing and one differentiating daughter cell. Mutations in Drosophila genes that disrupt the process of asymmetric division have been studied as models of neural-stem-cell derived cancers. Genes studied include numb, mira, pros, pins, brat, and l(3)mbt.

Loss-of-function mutations that affect normal neuroblast differentiation in Drosophila often result in conspicuous phenotypes of overproliferation and tumorous expansion of the brain at the larval stage. In brat or pros mutants, both daughter cells of the normally asymmetric division grow and behave like neuroblasts, leading to the formation of larval brain tumors comprised primarily of neuroblast-like cells. A sensitive test of neoplastic transformation is transplantation of potential tumorous cells into the abdomen of adult hosts; cells are observed for proliferation and invasive behaviors. One of the earliest genes characterized in this transplantation assay was brat. Homozygous loss-of-function mutations of numb, mira, pros, and pins have also been tested in this assay; larval brain tissue carrying neuroblasts with any of these mutations grew to more than 100 times their initial size, invading other tissues and killing the hosts in 2 weeks. The tumors became immortal and could be re-transplanted into new hosts for years.

See also reports for well-studied disease models using specific genes, 'cancer, neural stem cell, TRIM2,3-related' (FBhh0000772, using the fly gene brat) and 'cancer, neural stem cell, L3MBTL-related' (FBhh0000773, using the fly gene l(3)mbt).

[updated Jan. 2019 by FlyBase; FBrf0222196]

Disease Summary Information

Disease Summary: cancer, neural stem cell, disruption of asymmetric division

OMIM report

Human gene(s) implicated

Symptoms and phenotype

Genetics

Cellular phenotype and pathology

Molecular information

External links

Disease synonyms

Search term: neoplastic phenotype(s)

Search term: metastatic phenotype(s)

Related Diseases

Related human health report(s)

cancer, neural stem cell, L3MBTL-related

cancer, neural stem cell, TRIM2,3-related

Ortholog Information

Human gene(s) in FlyBase

Other mammalian ortholog(s) used

D. melanogaster Gene Information (6)

Dmel\numb

Gene Snapshot

numb (numb) encodes a membrane-associated inhibitor of Notch signaling. It controls neuroblast and sense organ precursor asymmetric division. It is asymmetrically localized during mitosis and segregates exclusively to one of two daughter cells. [Date last reviewed: 2019-03-14]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

NTCH-N

Comments on ortholog(s)

High-scoring ortholog of human NUMB and NUMBL (1 Drosophila to 2 human). Dmel\numb shares 39-41% identity and 49-51% similarity with the human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\mira

Gene Snapshot

miranda (mira) encodes a cytoplasmic and cortical scaffolding protein that binds the products of pros, stau and brat. It is asymmetrically localized to the basal cortex during neuroblast asymmetric cell division, resulting in its partioning into GMC daughter cells, where it is degraded and releases its cargo proteins. [Date last reviewed: 2019-03-14]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

No gene orthologous to mira has been identified in human.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\pros

Gene Snapshot

prospero (pros) encodes a protein that promotes the expression of neural differentiation genes and represses neuroblast stem cell identity genes and cell cycle proliferation genes; it is also important for astrocyte development. The product of pros is asymmetrically localized to the basal cortex during neuroblast asymmetric cell division, resulting in its partioning into GMC daughter cells, where it translocates into the nucleus. [Date last reviewed: 2019-03-14]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

PROS

Comments on ortholog(s)

Moderate-scoring ortholog of human PROX1 and PROX2 (1 Drosophila to 2 human). Dmel\pros shares 24-29% identity and 35-41% similarity with the human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\pins

Gene Snapshot

partner of inscuteable (pins) encodes a GDP-dissociation inhibitor involved in asymmetric cell division and mitotic spindle orientation. [Date last reviewed: 2019-06-13]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

High-scoring ortholog of human GPSM2 and GPMS1 (1 Drosophila to 2 human). Dmel\pins shares 45-47% identity and 60-63% similarity with the human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\brat

Gene Snapshot

brain tumor (brat) encodes a tumor suppressor that regulates proliferation in the brain. During neural stem cell division, it segregates into one daughter cell where it inhibits protein translation, preventing self-renewal and inducing differentiation. It also acts during oogenesis where it interacts with the products of nos and pum to suppress hb translation. [Date last reviewed: 2019-03-07]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Low-scoring ortholog of human TRIM3 and TRIM2 (2 Drosophila to 2 human). Dmel\brat shares 23-25% identity and 39-41% similarity with the human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\l(3)mbt

Gene Snapshot

lethal (3) malignant brain tumor (l(3)mbt) encodes a tumor suppressor protein regulating proliferation in the brain, particularly the optic lobes. The proposed functions of the product of l(3)mbt include histone compaction, establishment of chromatin insulators, regulation of the Hippo pathway and repression of the germ line program in somatic cells. [Date last reviewed: 2019-09-26]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Moderate-scoring ortholog of human L3MBTL3, L3MBTL4, and L3MBTL1 (1 Drosophila to 3 human). Dmel\l(3)mbt shares 28-31% identity and 42-46% similarity with the human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Synthetic Gene(s) Used (0)

Summary of Physical Interactions (98 groups)

numb

protein-protein

Interacting group

Assay

References

numb

anti tag coimmunoprecipitation, anti tag western blot

(Shan et al., 2018)

numb - AP-1γ

anti tag coimmunoprecipitation, western blot

(Cotton et al., 2013)

numb - AP-2α

anti tag coimmunoprecipitation, western blot, pull down, anti bait coimmunoprecipitation, autoradiography, anti tag western blot

(Cotton et al., 2013, Song and Lu, 2012, Wirtz-Peitz et al., 2008, Bhalerao et al., 2005, Berdnik et al., 2002)

numb - aPKC

pull down, western blot

(Wirtz-Peitz et al., 2008)

numb - baz

pull down, western blot

(Wirtz-Peitz et al., 2008)

numb - CRMP

anti tag coimmunoprecipitation, anti tag western blot

(Jauffred et al., 2013)

numb - Dap160

pull down, western blot

(Tang et al., 2005)

numb - Dronc

anti tag coimmunoprecipitation, anti tag western blot

(Ouyang et al., 2011)

numb - Eps-15

pull down, western blot, two hybrid

(Tang et al., 2005)

numb - mira

pull down, autoradiography

(Shen et al., 1998, Shen et al., 1997)

numb - mol

lambda phage display, pull down, western blot, anti tag coimmunoprecipitation, anti tag western blot, fluorescence polarization spectroscopy, predetermined participant

(Qin et al., 2004)

numb - N

two hybrid, pull down, autoradiography

(Guo et al., 1996)

numb - Nak

pull down, autoradiography, two hybrid, anti tag coimmunoprecipitation, western blot, molecular weight estimation by staining, cosedimentation, molecular weight, fluorescence polarization spectroscopy, predetermined participant, nuclear magnetic resonance

(Shan et al., 2018, Tang et al., 2005, Zwahlen et al., 2000, Chien et al., 1998, Li et al., 1998)

numb - par-6

pull down, western blot

(Wirtz-Peitz et al., 2008)

numb - Past1

phage display, tag visualisation, pull down, anti tag western blot

(Smith et al., 2004)

numb - pon

anti tag coimmunoprecipitation, western blot, anti tag western blot, x-ray crystallography, isothermal titration calorimetry, predetermined participant, pull down, molecular weight estimation by staining, two hybrid, cosedimentation, molecular weight, anti bait coimmunoprecipitation

(Shan et al., 2018, Ouyang et al., 2011, Bhalerao et al., 2005, Lu et al., 1998)

numb - spdo

anti tag coimmunoprecipitation, anti tag western blot, western blot, anti bait coimmunoprecipitation

(Cotton et al., 2013, Song and Lu, 2012, Tong et al., 2010, Bhalerao et al., 2005, Hutterer and Knoblich, 2005, O'Connor-Giles and Skeath, 2003)

RNA-RNA

Interacting group

Assay

References

numb - mir-ban

luminiscence technology, necessary binding region

(Wu et al., 2017)

mira

protein-protein

Interacting group

Assay

References

mira

protein cross-linking with a bifunctional reagent, molecular weight estimation by staining, detection by mass spectrometry, anti tag coimmunoprecipitation, western blot, comigration in gel electrophoresis

(Ramat et al., 2017, Yousef et al., 2008)

mira - aPKC

pull down, western blot, enzymatic study, autoradiography, anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation

(Atwood and Prehoda, 2009, Wirtz-Peitz et al., 2008)

mira - baz

pull down, anti tag western blot

(Wirtz-Peitz et al., 2008)

mira - brat

molecular sieving, molecular weight estimation by staining, two hybrid, isothermal titration calorimetry, predetermined participant, pull down, anti tag coimmunoprecipitation, anti tag western blot

(Liu et al., 2019, Jia et al., 2015, Komori et al., 2014, Lee et al., 2006)

mira - cup

two hybrid, pull down, western blot

(Piccioni et al., 2009)

mira - flfl

anti tag coimmunoprecipitation, western blot, two hybrid

(Sousa-Nunes et al., 2009)

mira - goddard

two hybrid

(Hu et al., 2017.6.13)

mira - insc

pull down, autoradiography

(Shen et al., 1998)

mira - jar

anti bait coimmunoprecipitation, peptide massfingerprinting, pull down, anti tag western blot, autoradiography, western blot

(Finan et al., 2011, Petritsch et al., 2003)

mira - numb

pull down, autoradiography

(Shen et al., 1998, Shen et al., 1997)

mira - par-6

pull down, western blot

(Wirtz-Peitz et al., 2008)

mira - pon

anti tag coimmunoprecipitation, western blot

(Lu et al., 1998)

mira - pros

anti bait coimmunoprecipitation, two hybrid, western blot, pull down, autoradiography

(Wirtz-Peitz et al., 2008, Petritsch et al., 2003, Shen et al., 1998, Ikeshima-Kataoka et al., 1997, Shen et al., 1997)

mira - stau

anti bait coimmunoprecipitation, western blot, isothermal titration calorimetry, predetermined participant, pull down, autoradiography, two hybrid, molecular sieving, x-ray crystallography, anti tag coimmunoprecipitation, anti tag western blot

(Jia et al., 2015, Petritsch et al., 2003, Schuldt et al., 1998, Shen et al., 1998)

mira - zip

anti bait coimmunoprecipitation, peptide massfingerprinting

(Petritsch et al., 2003)

RNA-protein

Interacting group

Assay

References

mira

(Ramat et al., 2017, Yousef et al., 2008)

mira - pros

anti bait coimmunoprecipitation, two hybrid, western blot, pull down, autoradiography

(Wirtz-Peitz et al., 2008, Petritsch et al., 2003, Shen et al., 1998, Ikeshima-Kataoka et al., 1997, Shen et al., 1997)

pros

RNA-protein

Interacting group

Assay

References

pros - Hrb87F

pull down, Identification by mass spectrometry

(Borah et al., 2009)

pros - Hrb98DE

pull down, Identification by mass spectrometry

(Borah et al., 2009)

pros - mira

anti bait coimmunoprecipitation, two hybrid, western blot, pull down, autoradiography

(Wirtz-Peitz et al., 2008, Petritsch et al., 2003, Shen et al., 1998, Ikeshima-Kataoka et al., 1997, Shen et al., 1997)

pros - stau

pull down, autoradiography, anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013, Li et al., 1997)

pros - Syp

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Samuels et al., 2020)

protein-protein

Interacting group

Assay

References

pros

anti tag coimmunoprecipitation, anti tag western blot

(Liu et al., 2020)

pros - abd-A

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018)

pros - C15

two hybrid array

(Shokri et al., 2019)

pros - Clbn

two hybrid, pull down, autoradiography

(Bi et al., 2005)

pros - CtBP

two hybrid array

(Shokri et al., 2019)

pros - CycE

anti tag coimmunoprecipitation, western blot

(Berger et al., 2010)

pros - mira

anti bait coimmunoprecipitation, two hybrid, western blot, pull down, autoradiography

(Wirtz-Peitz et al., 2008, Petritsch et al., 2003, Shen et al., 1998, Ikeshima-Kataoka et al., 1997, Shen et al., 1997)

pros - Oaz

two hybrid array

(Shokri et al., 2019)

pros - spoon

anti bait coimmunoprecipitation, western blot

(Tripathi et al., 2017)

pins

protein-protein

Interacting group

Assay

References

pins - aurA

enzymatic study, autoradiography

(Johnston et al., 2009)

pins - cnk

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

pins - cno

anti bait coimmunoprecipitation, western blot, pull down

(Wee et al., 2011, Speicher et al., 2008)

pins - dlg1

anti bait coimmunoprecipitation, western blot, pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, two hybrid, anti tag western blot

(Mauser and Prehoda, 2012, Siegrist and Doe, 2005, Bellaiche et al., 2001)

pins - dos

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

pins - drk

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

pins - Gαi

two hybrid, anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot, pull down, autoradiography

(Yoshiura et al., 2012, Kopein and Katanaev, 2009, Bowman et al., 2006, Hampoelz et al., 2005, Wang et al., 2005, Yu et al., 2005, Schaefer et al., 2001, Parmentier et al., 2000, Schaefer et al., 2000)

pins - Gαo

anti tag coimmunoprecipitation, western blot, fluorescent resonance energy transfer, experimental knowledge based, pull down, anti bait coimmunoprecipitation, two hybrid, autoradiography

(Lüchtenborg et al., 2015, Yoshiura et al., 2012, Guruharsha et al., 2011, Kopein and Katanaev, 2009, Katanaev and Tomlinson, 2006, Schaefer et al., 2000)

pins - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

pins - insc

anti bait coimmunoprecipitation, western blot, two hybrid, x-ray crystallography, static light scattering, molecular weight, anti tag coimmunoprecipitation, pull down, molecular weight estimation by staining, molecular sieving, autoradiography, peptide massfingerprinting

(Culurgioni et al., 2018, Januschke et al., 2013, Mauser and Prehoda, 2012, Culurgioni et al., 2011, Schaefer et al., 2000, Yu et al., 2000)

pins - Lkb1

anti bait coimmunoprecipitation, western blot

(Blumer et al., 2003)

pins - mud

pull down, autoradiography, molecular sieving, molecular weight estimation by staining, anti tag coimmunoprecipitation, anti tag western blot, molecular weight estimation by coomasie staining, anti bait coimmunoprecipitation, western blot, coimmunoprecipitation, fluorescence polarization spectroscopy, predetermined participant, molecular weight estimation by silver staining, Identification by mass spectrometry

(Mauser and Prehoda, 2012, Smith and Prehoda, 2011, Wang et al., 2011, Wee et al., 2011, Nipper et al., 2007, Bowman et al., 2006, Izumi et al., 2006, Siller et al., 2006)

pins - RasGAP1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

pins - RhoGAP18B

experimental knowledge based

(Guruharsha et al., 2011)

pins - ric8a

anti bait coimmunoprecipitation, western blot, pull down

(Wang et al., 2005)

pins - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

brat

protein-protein

Interacting group

Assay

References

brat

x-ray crystallography

(Liu et al., 2019)

brat - AGO1

anti bait coimmunoprecipitation, western blot, two hybrid, anti tag coimmunoprecipitation, anti tag western blot, bimolecular fluorescence complementation, fluorescence microscopy, peptide massfingerprinting

(Malik et al., 2017, Neumuller et al., 2008)

brat - bam

bimolecular fluorescence complementation, fluorescence microscopy, two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Malik et al., 2017)

brat - eIF4EHP

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot

(Cho et al., 2006)

brat - mira

molecular sieving, molecular weight estimation by staining, two hybrid, isothermal titration calorimetry, predetermined participant, pull down, anti tag coimmunoprecipitation, anti tag western blot

(Liu et al., 2019, Jia et al., 2015, Komori et al., 2014, Lee et al., 2006)

brat - nos

two hybrid

(Sonoda and Wharton, 2001)

brat - pum

pull down, western blot, anti tag western blot, three hybrid, molecular sieving, molecular weight estimation by staining, transcriptional complementation assay

(Loedige et al., 2014, Kadyrova et al., 2007, Cho et al., 2006, Edwards et al., 2001, Sonoda and Wharton, 2001)

brat - sstn

anti tag coimmunoprecipitation, peptide massfingerprinting

(Liu et al., 2015)

brat - stau

pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, primer specific pcr

(Jia et al., 2015, Loedige et al., 2015)

brat - Sxl

two hybrid, bimolecular fluorescence complementation, fluorescence microscopy, anti tag coimmunoprecipitation, anti tag western blot

(Malik et al., 2017)

brat - Tis11

anti tag coimmunoprecipitation, anti tag western blot

(Komori et al., 2018)

RNA-protein

Interacting group

Assay

References

brat - aub

iclip, partial RNA sequence identification

(Ma et al., 2017)

brat - chinmo

anti tag coimmunoprecipitation, primer specific pcr

(Loedige et al., 2015)

brat - Corin

anti tag coimmunoprecipitation, primer specific pcr

(Loedige et al., 2015)

brat - dpn

anti tag coimmunoprecipitation, primer specific pcr, electrophoretic mobility shift assay, autoradiography

(Reichardt et al., 2018, Loedige et al., 2015)

brat - elav

anti bait coimmunoprecipitation, primer specific pcr

(Hilgers et al., 2012)

brat - hb

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, molecular sieving, static light scattering, nucleic acid uv cross-linking assay, Identification by mass spectrometry, microscale thermophoresis, predetermined participant, electrophoretic mobility shift assay, autoradiography

(Malik et al., 2019, Loedige et al., 2014)

brat - klu

electrophoretic mobility shift assay, autoradiography, anti tag coimmunoprecipitation, primer specific pcr

(Loedige et al., 2015)

brat - kni

electrophoretic mobility shift assay, autoradiography, anti tag coimmunoprecipitation, primer specific pcr

(Loedige et al., 2015)

brat - Mad

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Malik et al., 2019)

brat - Myc

anti tag coimmunoprecipitation, primer specific pcr

(Loedige et al., 2015)

brat - orb2

pull down, western blot

(Mastushita-Sakai et al., 2010)

brat - par-6

anti tag coimmunoprecipitation, primer specific pcr

(Loedige et al., 2015)

brat - Pc

anti tag coimmunoprecipitation, primer specific pcr

(Loedige et al., 2015)

brat - piwi

anti bait coimmunoprecipitation, northern blot

(Saito et al., 2009)

brat - Sirt1

anti tag coimmunoprecipitation, primer specific pcr

(Loedige et al., 2015)

brat - stau

pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, primer specific pcr

(Jia et al., 2015, Loedige et al., 2015)

brat - w

anti tag coimmunoprecipitation, primer specific pcr

(Loedige et al., 2015)

brat - zld

electrophoretic mobility shift assay, autoradiography, anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Reichardt et al., 2018)

l(3)mbt

protein-protein

Interacting group

Assay

References

l(3)mbt - Ada2b

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

l(3)mbt - Atac3

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

l(3)mbt - CoRest

anti tag coimmunoprecipitation, western blot, molecular sieving, ion exchange chromatography, anti bait coimmunoprecipitation, Identification by mass spectrometry

(Mačinković et al., 2019, Meier et al., 2012)

l(3)mbt - Cp190

two hybrid

(Melnikova et al., 2019)

l(3)mbt - E2f1

anti tag coimmunoprecipitation, western blot

(Lu et al., 2007)

l(3)mbt - HDAC1

coimmunoprecipitation, western blot

(Scharf et al., 2009)

l(3)mbt - His4

pull down, western blot

(Scharf et al., 2009)

l(3)mbt - Lint-1

affinity technology, peptide massfingerprinting, anti tag coimmunoprecipitation, western blot, pull down, autoradiography, molecular sieving, ion exchange chromatography, anti bait coimmunoprecipitation, experimental knowledge based

(Rhee et al., 2014, Meier et al., 2012)

l(3)mbt - mip120

pull down, autoradiography

(Blanchard et al., 2014)

l(3)mbt - Scm

tandem affinity purification, Identification by mass spectrometry

(Kang et al., 2015)

l(3)mbt - Su(H)

pull down, autoradiography

(Xu et al., 2017)

Alleles Reported to Model Human Disease (Disease Ontology) (7 alleles)

mira

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

mira^HMS00347

exacerbates central nervous system cancer

modeled by N^UAS.icd

(Zacharioudaki et al., 2016)

pros

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

pros¹⁷

exacerbates chronic myeloid leukemia

modeled by Hsap\ABL1::Hsap\BCR^UAS.cBa

(Bernardoni et al., 2019)

ameliorates non-syndromic X-linked intellectual disability

modeled by Acsl⁰⁵⁸⁴⁷, Acsl⁸

(Jia et al., 2019)

brat

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

brat^HMS01121

model of cancer

CEA

(Hussain et al., 2017)

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

brat^HMS01121

model of cancer

is ameliorated by Pepck1^GD9451

(Hussain et al., 2017)

is ameliorated by Mfe2^KK101732

(Hussain et al., 2017)

is ameliorated by Gpdh1^GD14102

(Hussain et al., 2017)

is ameliorated by Mdh1^GD11625

(Hussain et al., 2017)

is ameliorated by Men^GL01234

(Hussain et al., 2017)

brat¹

exacerbates viral infectious disease

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates infectious mononucleosis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates Epstein-Barr virus hepatitis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

brat^k06028

exacerbates viral infectious disease

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates infectious mononucleosis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates Epstein-Barr virus hepatitis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

l(3)mbt

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

l(3)mbt^ts1

model of brain cancer

CEA

(Gateff et al., 1993)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

l(3)mbt^HMS01466

ameliorates amyotrophic lateral sclerosis type 1

modeled by Hsap\SOD1^A4V.UAS

(Lu et al., 2019)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis-1

modeled by Zzzz\poly-GR^PO-100.UAS

(Lu et al., 2019)

modeled by Zzzz\poly-PR^PO-100.UAS

(Lu et al., 2019)

Genetic Tools, Stocks and Reagents

Sources of Stocks

Contact lab of origin for a reagent not available from a public stock center.

Bloomington Stock Center Disease Page

Selected mammalian transgenes

Allele

Transgene

Publicly Available Stocks

Selected Drosophila transgenes

Allele

Transgene

Publicly Available Stocks

RNAi constructs available

Allele

Transgene

Publicly Available Stocks

numb^{dsRNA.UAS.Tag:MYC}

P{UAS-numb-Myc.dsRNA}

numb^JF01695

P{TRiP.JF01695}

y¹ v¹; P{TRiP.JF01695}attP2

numb^HMS01459

P{TRiP.HMS01459}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01459}attP2

numb^NIG.3779R

P{NIG.3779R}

numb^{dsRNA.UAS.THU1674}

P{UAS-numb.RNAi.THU1674}

brat^GD7073

P{GD7073}

w¹¹¹⁸ P{GD7073}v31333

y¹ v¹; P{TRiP.HM05078}attP2

brat^HMS01121

P{TRiP.HMS01121}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01121}attP2

w¹¹¹⁸; P{GD1596}v51484/CyO

mira^HMS00347

P{TRiP.HMS00347}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00347}attP2

w¹¹¹⁸; P{GD4370}v12709

y¹ v¹; P{TRiP.JF02913}attP2

l(3)mbt^HMS01466

P{TRiP.HMS01466}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01466}attP2/TM3, Sb¹

w¹¹¹⁸; P{GD11737}v27486/TM3

pins^JF02450

P{TRiP.JF02450}

y¹ v¹; P{TRiP.JF02450}attP2

pins^HMS01462

P{TRiP.HMS01462}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01462}attP2

pins^GL00622

P{TRiP.GL00622}

y¹ sc^* v¹ sev²¹; P{TRiP.GL00622}attP40

pins^HMJ21351

P{TRiP.HMJ21351}

y¹ v¹; P{TRiP.HMJ21351}attP40

y¹ v¹; P{TRiP.JF02308}attP2/TM3, Sb¹

y¹ v¹; P{TRiP.HMJ02107}attP40

pros^VSH330051

P{VSH330051}

P{VSH330051}attP40

Selected Drosophila classical alleles

Allele

Allele class

Mutagen

Publicly Available Stocks

numb¹

amorphic allele - genetic evidence

PM hybrid dysgenesis

numb¹/CyO

numb²

amorphic allele - genetic evidence

diepoxybutane

numb^S52F

loss of function allele

numb¹⁵

amorphic allele - genetic evidence

ethyl methanesulfonate

numb³

amorphic allele - genetic evidence

diepoxybutane

brat^k06028

P-element activity

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}brat^k06028 P{neoFRT}40A/CyO y⁺

brat¹

ethyl methanesulfonate

brat¹ rdo¹ hook¹ pr¹/CyO

brat¹¹

amorphic allele - molecular evidence

formaldehyde

brat^2L-150-11

amorphic allele - molecular evidence

ethyl methanesulfonate

brat^2L-192-9

amorphic allele - molecular evidence

ethyl methanesulfonate

mira^KO

loss of function allele

cre recombinase

mira^L44

loss of function allele

ethyl methanesulfonate

w^*; P{neoFRT}82B mira^L44 e^*/TM6B, P{Dfd-EYFP}3, Sb¹ Tb¹ ca¹

mira^ZZ176

loss of function allele

ethyl methanesulfonate

l(3)mbt^ts1

ethyl methanesulfonate

pins^P120

amorphic allele - molecular evidence

Delta2-3 transposase

pins^P62

loss of function allele

Delta2-3 transposase

pins^P17

amorphic allele - molecular evidence

Delta2-3 transposase

pins^P89

loss of function allele

Delta2-3 transposase

pros¹³⁹

loss of function allele

P-element activity

pros^C43

amorphic allele - genetic evidence

Delta2-3 transposase

pros^V17

loss of function allele

Delta2-3 transposase

pros^m1

amorphic allele - genetic evidence

ethyl methanesulfonate

pros¹⁷

amorphic allele - genetic evidence

P-element activity

w^*; pros¹⁷/TM6B, Tb¹

pros^m2

amorphic allele - genetic evidence

ethyl methanesulfonate

pros^JO1

amorphic allele - genetic evidence

P-element activity

pros^m5

amorphic allele - genetic evidence

ethyl methanesulfonate

pros^JO13

amorphic allele - genetic evidence

P-element activity

pros¹⁴

amorphic allele - genetic evidence

P-element activity

pros^m4

amorphic allele - genetic evidence

ethyl methanesulfonate

pros^K816

amorphic allele - genetic evidence

ethyl methanesulfonate

pros^Il3

loss of function allele

pros^C7

amorphic allele - genetic evidence

Delta2-3 transposase

pros^m3

amorphic allele - genetic evidence

ethyl methanesulfonate

pros^D135

amorphic allele - genetic evidence

ethyl methanesulfonate

References (15)

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