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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000071509
Interaction Network
Interactions Browser links
Tsc1 network
gig network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0135685-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tsc1 

Tsc1

bait

GST tag

Tsc1 
gig 

Tsc2

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tsc1 
C terminal region
necessary binding region
aa 673-1100
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0136941-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tsc1 

TSC1

bait

Myc tag

gig 

TSC2

prey

V5 tag, His tag

gig 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gig 
N-terminal half
unspecified role
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transiently transfected cells.

Prey expressed in transiently transfected cells.

FBrf0180255-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gig 

Tsc2

bait

Pyo tag

Tsc1 

Tsc1

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Tsc2 variations to nonphosphorylatable alanine (S924A and T1518A) or phosphomimicking residues (S924D and T1518E) had no effect on the interaction.

FBrf0180255-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gig 

Tsc2

bait
Tsc1 

Tsc1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene

Presence of insulin resulted in a mobility shift of Tsc2 but absence of insulin did not affect interaction.

FBrf0216604-36.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tsc1 

Tsc1

bait

GFP tag

gig 

gig

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

SAINT score = 1

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0216604-96.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gig 

gig

bait

GFP tag

Tsc1 

Tsc1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

SAINT score = 1

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0233454-1722.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tsc1 

Tsc1

bait

TAP tag

gig 

gig

prey

SAINT score = 0.9963

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

Observed in two of three conditions tested: 10 minutes and 30 minutes after insulin stimulation.

FBrf0233454-456.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gig 

gig

bait

TAP tag

Tsc1 

Tsc1

prey

SAINT score = 0.997

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

Observed in all three conditions tested: uninduced cells, 10 minutes after insulin stimulation and 30 minutes after insulin stimulation.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)