brm, mor, dalao, Hsc70-4, Bap60, Bap55, Snr1 and Bap47 (actin) were identified as components of the Brahma complex.

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Nuclear extract was fractionated through several different chromatographic steps, selecting for brm-containing fractions, before pull down of His tagged brm.

This interaction persists in the presence of ethidium bromide, and so is not due to indirect interactions via DNA.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

This interaction persists in the presence of ethidium bromide, and so is not due to indirect interactions via DNA.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated on a glycerol gradient and fractions containing brm and osa were used for immunoprecipitation.

Interaction in vitro; bait derived from wild type embryonic nuclear extract; prey produced and labeled by in vitro translation.

Purified Brahma complex was resolved by SDS-PAGE, transferred to a blot and proteins were renatured. The blot was then incubated with radiolabeled prey protein.

brm, osa, Bap170, p400, mor, dalao, Bap60, Bap55, Snr1, Bap47 (actin) and Bap26 were identified as components of the Brahma complex.

Source was embryonic nuclear extract of wild-type fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Nuclear extract was fractionated through several different chromatographic steps, selecting for brm-containing fractions, before immunoprecipitation of brm.

mor is present with brm in all Brahma complexes.

Source was embryonic nuclear extract wild-type fly line; proteins produced from endogenous gene.

Nuclear extract was subjected to several chromatographic steps to isolate brm-containing complexes.

brm, mor, dalao, Bap60, Bap55, Snr1 and actin form the Brahma (BRM) complex, together with either osa (BAP BRM complex) or polybromo and Bap170 (PBAP BRM complex).

Source was embryonic nuclear extract wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated by ammonium sulfate precipitation and several chromatographic steps before being subjected to immunoprecipitation.

BAP complex purified via Bap60-HA immunoprecipitation, cross-links detected by mass spectrometry.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

Positive control.

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.