Source was HEK 293T (human) cell line transfected with tagged Atg1 and sqa constructs.

Use a kinase substrate-trapping approach in which the catalytically inactive kinase variants formed a stable interaction with their substrates (Deminoff et al., 2006, Genetics 173: 1909–1917).

Interaction detectable only with kinase-inactive form of Atg1; not detected with wild-type Atg1 protein.

Source was HEK 293T (human) cell line transfected with tagged Atg1 and sqa constructs.

Use a kinase substrate-trapping approach in which the catalytically inactive kinase variants formed a stable interaction with their substrates (Deminoff et al., 2006, Genetics 173: 1909–1917).

Interaction assayed with kinase-inactive form of Atg1.