Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000097492
Interaction Network
Interactions Browser links
robo1 network
sli network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0107628-1.AT.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

dRobo1

bait
sli 

dSlit

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from transfected COS cells; prey purified from medium of transfected S2 cells.

robo-transfected COS cells wre incubated with supernatants from sli-transfected S2 cells. Binding to the cell surface was determined by immunofluorescence using sli antibody.

FBrf0107628-2.AT.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

dSlit

bait

dRobo1

prey

Fc tag, [125]I label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ectodomain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from transfected COS cells; prey purified from medium of transfected COS cells.

sli and robo bind with a K[[d]] = 41.7 nM.

sli-transfected COS cells wre incubated with purified Fc-tagged robo, then with [125]I anti-human IgG. Total binding to the cell surface was determined by measuring cell-associated radioactivity.

FBrf0136993-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

slit

bait

Biotin tag

sli 

Robo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sli 
LRR domain
necessary binding region

coordinates relative to sli-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0180200-5.ELISA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

Slit

bait

His, Myc tag

sli 
sli 
sli 
sli 
sli 

Robo

prey

Fc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
IG domains 1-5
sufficient binding region
aa 51-546

"D1-5", coordinates relative to robo1-PA

sli 
LRR domain 2
sufficient binding region
aa 314-542

"D2", coordinates relative to sli-PC

sli 
LRR domain 2 residue Y402
mutation disrupting interaction
aa 402

Y402S, coordinates relative to sli-PC

sli 
LRR domain 2 residue L424
mutation disrupting interaction
aa 424

L424R, coordinates relative to sli-PC

sli 
LRR domain 2 residue Y450
mutation disrupting interaction
aa 450

Y450S, coordinates relative to sli-PC

sli 
LRR domain 2 residue H472
mutation disrupting interaction
aa 472

H472S, coordinates relative to sli-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait purified from conditioned medium of transfected 293 cells; prey purified from conditioned medium of transfected 293 cells.

FBrf0200053-1.ELISA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

Slit

bait

His tag

sli 

Robo

prey

Fc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
IG1-5 domains
sufficient binding region
aa 51-546

coordinates relative to robo1-PA

sli 
LRR1-4 domains
sufficient binding region
aa 72-934

coordinates relative to sli-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait purified from conditioned medium of transfected 293-EBNA cells; prey purified from conditioned medium of transfected 293-EBNA cells.

The robo1-sli interaction is enhanced by heparin.

FBrf0200053-2.CH
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

Slit

neutral component

His tag

sli 

Slit

neutral component

His tag

sli 
sli 

Slit

neutral component

His tag

sli 

Robo

neutral component

Fc tag

Robo

neutral component

Fc tag

Robo

neutral component

Fc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
IG domains 1-2
sufficient binding region
aa 51-254

"IG1-2", coordinates relative to robo1-PA

sli 
LRR domain 2
sufficient binding region
aa 314-542

"D2", coordinates relative to sli-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait purified from conditioned medium of transfected 293-EBNA cells; prey purified from conditioned medium of transfected 293-EBNA cells.

The robo1-sli constructs form a binary complex with 1:1 stoichiometry. A ternary 1:1:1 complex is formed with a purified heparin-derived decasaccaharide.

FBrf0205517-1.ELISA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

Slit

bait

His, Myc tag

sli 

Robo

prey

Fc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sli 
LRR domain 2
sufficient binding region
aa 314-542

"D2", coordinates relative to sli-PC

Ig domains 1-5
sufficient binding region
aa 51-546

"D1-5", coordinates relative to robo1-PA

Ig domain 1 residue T74
mutation disrupting interaction
aa 74

T74E, coordinates relative to robo1-PA

Ig domain 1 residue F114
mutation disrupting interaction
aa 114

F114E, coordinates relative to robo1-PA

Ig domain 1 residue R117
mutation disrupting interaction
aa 117

R117E, coordinates relative to robo1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait purified from conditioned medium of transfected 293 cells; prey purified from conditioned medium of transfected 293 cells.

Mutations in robo1 Ig domain 2 have only a marginal effect on sli binding.

FBrf0210412-1.SPR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Robo1

neutral component

His tag

Robo1

neutral component

His tag

Robo1

neutral component

His tag

sli 

Slit

neutral component

His tag

sli 
sli 

Slit

neutral component

His tag

sli 

Slit

neutral component

His tag

sli 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ig domains 1-5
sufficient binding region
sli 
Robo-binding domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus system; prey produced as a recombinant fusion protein in baculovirus system.

sli binds robo (Kd = 235nM) more strongly than lea/robo2 (Kd = 1098nM).

FBrf0221963-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

Slit

bait

Robo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0229066-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Robo1

bait

Myc tag

sli 

Slit

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey derived from conditioned medium.

FBrf0230014-1.AT.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Robo1

bait

HA tag

sli 

slit

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ig1 domain
necessary binding region
aa 1-152

coordinates relative to robo1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait present on live, transfected S2R+ cells; prey produced from conditioned media of transfected S2R+ cell line.

S2R+ cells expressing robo1 were incubated with sli-conditioned medium, then fixed and analyzed for sli-surface binding by immunofluorescence.

FBrf0233216-1.AT.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Robo1

bait

HA tag

sli 

slit

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ig1 domain
necessary binding region
aa 1-152

coordinates relative to robo1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait present on live, transfected S2R+ cells; prey produced from conditioned media of transfected S2R+ cell line.

Only robo1 Ig1 domain is necessary for sli binding (other Ig domains tested are dispensable).

S2R+ cells expressing robo1 were incubated with sli-conditioned medium, then fixed and analyzed for sli-surface binding by immunofluorescence.

FBrf0233519-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Robo1

bait
sli 

Slit

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293 cell line; bait produced from transfected construct; prey produced from transfected construct.

In the absence of Dscam1, robo1 preferentially interacts with full length sli. Upon cotransfection of Dscam1, robo1 preferentially binds the proteolytically processed sliN form.

FBrf0235927-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

Slit

bait

Robo1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The sli-robo1 interaction occurs in both wildtype and mmy mutant backgrounds.

FBrf0237629-1.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

Slit

bait

Robo1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Interaction is detected in wildtype and in ptc mutant embryos.

FBrf0237629-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Robo1

bait
sli 

Slit

prey
sli 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sli 
N-terminal domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Precipitation of robo1 coprecipitates full length sli,the processed N-terminal fragment of sli but not the C-terminal fragment.

FBrf0238021-1.AT.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Robo1

bait

HA tag

sli 

Slit

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction was observed on the surface of SR2+ cells expressing robo1. sli was derived from the medium of transfected S2R+ and bound sli was detected with anti-sli antibodies.

Source was S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (14)