This interaction persists upon RNase treatment.
Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was Drosophila nuclear extracts, stage unspecified; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
weak interaction
Source was whole cell extracts; bait produced from endogenous gene; prey produced from endogenous gene.
weak interaction
Source was whole cell extracts; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of Sg4 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Positive control.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; proteins produced as a recombinant fusion proteins in a baculovirus and insect cell system.
Positive control.
Proteins were expressed individually in Sf21 cells and whole cell extracts were mixed.
Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system; prey produced as recombinant fusion protein in baculovirus-infected Sf21 cell system.
Source was cross-linked nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Crude nuclear extracts were cross-linked using formaldehyde, sonicated, and subjected to ProteinA affinity purification with rabbit IgG agarose beads eluted under denaturing conditions, and subsequently using streptavidin agarose beads for Bio affinity purification. Purified peptides were eluted by on-bead trypsin digestion and mass spectrometric analysis.
Enrichment of prey protein was compared to input, mock and unrelated BioTAP affinity purifications using a statistical method (Bamse) developed by the authors to control for multiple sources of bias.
Source was cross-linked nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Positive control.
One step, IgG-mediated pulldown of ProteinA portion of BioTAP tag.
Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.
HGScore = 14.41017
Factors were detected on polytene chromosomes using antibodies (to proteins) or biotinylated antisense probes (to ncRNA). The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).
Source was larval salivary gland of wild-type fly line; proteins produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
K532R,K539R,K541R,K545R,K694R,K706R,K715R,K773R,K776R
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was embryos of engineered fly line; bait produced from tagged endogenous gene; prey produced from endogenous gene.
Source was ovaries of engineered fly line; bait produced from tagged endogenous gene; prey produced from endogenous gene.
