msl-2, Unr Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

msl-2, Unr

FlyBase ID

FBig0000098789

Interaction Network

Interactions Browser links

msl-2 network
Unr network

Reported Interactions

FBrf0190533-5.XL.A

Reference

Abaza et al., 2006

Description

physical association

Assay

nucleic acid uv cross-linking assay

autoradiography

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

msl-2

bait

[32]P label

msl-2

Unr

dUNR

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

msl-2

3-prime UTR

sufficient binding region

UUUUUUUGAGCAUGAAUUUUUUUGAGCACGUGAACCUAGGAUUAAG

msl-2

3-prime UTR

mutation disrupting interaction

UUUUUUUGAGCAUGAAUUUUUUUGAGCACGUGAACCUAGGAUUAAG

mut2456, mutated to UUUUUUUGCUCUCUCAUUUUUUUGCUCUCUCUCUCUCUCUCUCUCU

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type embryo extract.

(Abaza et al., 2006)

Unr binding to msl-2 RNA is weak but strongly enhanced by the addition of a recombinant purified Sxl RNA-binding domains fragment.

(Abaza et al., 2006)

FBrf0190533-7.EMSA.A

Reference

Abaza et al., 2006

Description

physical association

Assay

electrophoretic mobility shift assay

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Unr

dUNR

bait

His tag

msl-2

prey

[32]P label

msl-2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

msl-2

3-prime UTR

sufficient binding region

UUUUUUUGAGCAUGAAUUUUUUUGAGCACGUGAACCUAGGAUUAAG

msl-2

3-prime UTR

mutation disrupting interaction

UUUUUUUGAGCAUGAAUUUUUUUGAGCACGUGAACCUAGGAUUAAGUUUUUUUGAGCAUGAAUUUUUUUGAGCACGUGAACCUAGGAUUAAG

EFmut, mutated to CUCUCUCUAGCAUGAACUCUCUCUAGCACGUGAACCUAGGAUUAAG

mut2456, mutated to UUUUUUUGCUCUCUCAUUUUUUUGCUCUCUCUCUCUCUCUCUCUCU

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Abaza et al., 2006)

FBrf0190533-8.coIP.PCR

Reference

Abaza et al., 2006

Description

physical association

Assay

anti bait coimmunoprecipitation

quantitative reverse transcription pcr

Collection

Source/Stage

organism / female adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Unr

dUNR

bait

msl-2

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult females; bait produced from endogenous gene; prey produced from endogenous gene.

(Abaza et al., 2006)

FBrf0190542-1.CH.MS

Reference

Duncan et al., 2006

Description

physical association

Assay

affinity chromatography technology

Identification by mass spectrometry

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

msl-2

bait

Box-B hairpin tag

msl-2

Unr

UNR

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

msl-2

5-prime and 3-prime UTR

sufficient binding region

nt 270-339,909-954

msl-2

5-prime and 3-prime UTR

mutation disrupting interaction

for site B in 5-prime UTR, T16 mutated to (CT)8; for sites E,F in 3-prime UTR, U7G mutated to (CT)4

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.

(Duncan et al., 2006)

FBrf0190542-2.CH.WB

Reference

Duncan et al., 2006

Description

physical association

Assay

affinity chromatography technology

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

msl-2

bait

Box-B hairpin tag

msl-2

Unr

UNR

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

msl-2

5-prime and 3-prime UTR

sufficient binding region

nt 270-339,909-954

msl-2

5-prime and 3-prime UTR

mutation disrupting interaction

for site B in 5-prime UTR, T16 mutated to (CT)8; for sites E,F in 3-prime UTR, U7G mutated to (CT)4

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.

(Duncan et al., 2006)

Addition of purified recombinant Sxl increases the msl-2-Unr interaction.

(Duncan et al., 2006)

FBrf0221878-12.PD.WB

Reference

Graindorge et al., 2013

Description

physical association

Assay

pull down

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

msl-2

Msl-2

bait

biotin label

msl-2

Unr

UNR

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

msl-2

3-prime UTR EF site

sufficient binding region

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Positive control.

(Graindorge et al., 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Graindorge et al., 2013)

FBrf0221878-2.PD.WB

Reference

Graindorge et al., 2013

Description

physical association

Assay

pull down

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

msl-2

bait

biotin label

msl-2

Unr

UNR

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

msl-2

3-prime UTR EF sites

sufficient binding region

msl-2

3-prime UTR EF sites

necessary binding region

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

GRAB (GST pull-down and RNA affinity binding) was used to identify components of the Sxl-msl-2 mRNP complex. GST-Sxl and biotinylated msl-2 RNA were mixed with embryonic extract. mRNP complexes were purified by GST-glutathione, then by biotin-streptavidin affinity steps.

(Graindorge et al., 2013)

Positive control.

(Graindorge et al., 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Graindorge et al., 2013)

FBrf0226752-2.EMSA.A

Reference

Hennig et al., 2014

Description

physical association

Assay

electrophoretic mobility shift assay

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Unr

bait

His tag

Unr

msl-2

prey

[32]P label

msl-2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

msl-2

Sxl-binding sites EF

sufficient binding region

UUUUUUUGAGCAUGAAUUUUUUUGAGCACGUGAA

msl-2

Sxl-binding sites EF

mutation decreasing interaction

UUUUUUUGAGCAUGAAUUUUUUUGAGUACGUGAA

Unr

CSD1 domain

sufficient binding region

aa 185-252

coordinates refer to Unr-PA

Unr

triple zipper domain

mutation decreasing interaction

aa 213aa 239

H213A, coordinates refer to Unr-PA

R239A, coordinates refer to Unr-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Hennig et al., 2014)

Interaction shown as supershift of msl-2-Sxl complex.

(Hennig et al., 2014)

FBrf0226752-4.XC

Reference

Hennig et al., 2014

Description

physical association

Assay

x-ray crystallography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Unr

neutral component

Unr

neutral component

Unr

neutral component

Unr

msl-2

neutral component

msl-2

neutral component

msl-2

neutral component

msl-2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

msl-2

Sxl-binding site F

sufficient binding region

UUUUUUUGAGCACGUGAA

Unr

CSD1 domain

sufficient binding region

aa 181-252

coordinates refer to Unr-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; protein produced as recombinant fusion protein in bacterial system; mRNA produced by in vitro transcription.

(Hennig et al., 2014)

Structure of Sxl, Unr, msl-2 ternary complex shown. Captured as two protein-mRNA interactions since Sxl and Unr do not interact in the absence of mRNA.

(Hennig et al., 2014)

FBrf0226752-6.SLS

Reference

Hennig et al., 2014

Description

physical association

Assay

static light scattering

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Unr

neutral component

Unr

neutral component

Unr

neutral component

Unr

msl-2

neutral component

msl-2

neutral component

msl-2

neutral component

msl-2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

msl-2

Sxl-binding sites F

sufficient binding region

UUUUUUUGAGCACGUGAA

Unr

CSD1 domain

sufficient binding region

aa 181-252

coordinates refer to Unr-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; protein produced as recombinant fusion protein in bacterial system; mRNA produced by in vitro transcription.

(Hennig et al., 2014)

Ternanry Sxl, Unr, msl-2 complex analyzed. Captured as two protein-mRNA interactions since Sxl and Unr do not interact in the absence of mRNA.

(Hennig et al., 2014)

FBrf0226752-7.ITC

Reference

Hennig et al., 2014

Description

physical association

Assay

isothermal titration calorimetry

predetermined participant

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Unr

bait

Unr

msl-2

prey

msl-2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

msl-2

Sxl-binding sites F

sufficient binding region

UUUUUUUGAGCACGUGAA

Unr

CSD1 domain

sufficient binding region

aa 181-252

coordinates refer to Unr-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; protein produced as recombinant fusion protein in bacterial system; mRNA produced by in vitro transcription.

(Hennig et al., 2014)

FBrf0238839-2.EMSA.A

Reference

Szostak et al., 2018

Description

physical association

Assay

electrophoretic mobility shift assay

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Unr

UNR

bait

His, FLAG tag

msl-2

prey

[32]P label

msl-2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

msl-2

3-prime UTR

sufficient binding region

UUUUUUUGAGCAUGAAUUUUUUUGAGCACGUGAACCUAGGAUUAAG

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Unr binding to msl-2 RNA was observed as a decrease in mobility of a Sxl-msl-2 complex upon adding Unr.

(Szostak et al., 2018)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Szostak et al., 2018)

FBrf0238839-6.CH.WB

Reference

Szostak et al., 2018

Description

physical association

Assay

affinity chromatography technology

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

msl-2

bait

biotin label

msl-2

Unr

UNR

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

msl-2

3-prime UTR

sufficient binding region

UUUUUUUGAGCAUGAAUUUUUUUGAGCACGUGAACCUAGGAUUAAG

msl-2

3-prime UTR

mutation disrupting interaction

UUUUUUUGAGCAUGAAUUUUUUUGAGCACGUGAACCUAGGAUUAAG

UUUUUUUG to (CU)4

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Recombinant GST-Sxl RNA binding domain 4 was added to RNA affinity column along with embryo extract.

(Szostak et al., 2018)

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type embryo extract.

(Szostak et al., 2018)

External Crossreferences and Linkouts ( 1 )

Linkouts

MIST - An integrated Molecular Interaction Database

msl-2,Unr

References (5)

Report Sections

Open Close