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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000099362
Interaction Network
Interactions Browser links
Mer network
wts network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0222652-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mer 

Mer

bait

HA tag

Mer 
Mer 
wts 

Wts

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mer 
FERM domain
sufficient binding region
aa 1-375
Mer 
C-terminal region
mutation increasing interaction
aa 601-635

ΔC-terminus

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

wts coimmunoprecipitates with Mer but not ex in this assay.

The C-terminus of Mer inhibits the interaction with wts and presumably regulates Mer activity.

Mer promotes membrane association of wts.

The interaction of wts with full length Mer is enhanced by treatment with drugs that disrupt the actin cytoskeleton (C3, Latrunculin B).

FBrf0222652-5.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mer 

Mer

bait
wts 

Wts

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from endogeous gene; prey produced from endogeous gene.

FBrf0222652-7.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wts 

Wts

bait

V5 tag

Mer 

Mer

prey

HA tag

Mer 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mer 
FERM domain
sufficient binding region
aa 1-375
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

wts coimmunoprecipitates the N-terminal half of Mer, but not full length Mer.

FBrf0222652-8a.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mer 

Mer

bait

HA tag

Mer 
wts 

Wts

prey

GFP tag

wts 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mer 
FERM domain
sufficient binding region
aa 1-375
wts 
N-terminal region
sufficient binding region
aa 1-50
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

The interaction of wts with the Mer FERM domain is inhibited by co-transfection of a separate construct bearing the Mer C-terminus (aa 375-635).

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)