Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103322
Interaction Network
Interactions Browser links
chico network
InR network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0130030-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
InR 

dIRc

bait

fused to LexA DNA-binding domain

InR 
InR 
InR 

dIRS

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
InR 
NPXY motifs
sufficient binding region
aa 1328-2144
InR 
intracellular domain
necessary binding region
aa 1662-2144
InR 
intracellular domain
mutation decreasing interaction
aa 1962-2144

deletion of this region decreases binding by 70%

N-terminal region
sufficient binding region
aa 1-410
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42-AD

FBrf0204162-3.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Chico

bait

HA tag

InR 

Insulin-like receptor precursor

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The enrichment of candidate chico-interacting proteins in control versus experimental samples was corrected for relative protein abundance in the lysates. Only three chico-interacting candidates were considered to be enriched above the threshold expected for abundant contaminant proteins: 14-3-3ε, 14-3-3ζ and InR.

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0216604-44.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

chico

bait

GFP tag

InR 

InR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

SAINT score = 1

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0221426-1.FRET
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
InR 

InR

bait

CFP tag

Chico

prey

RFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The InR-chico interaction is observed in 71% of samples upon insulin stimulation of tissue (compared to 20% of samples in the absence of insulin).

Source was larval salivary gland of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

FBrf0224702-229.coIP.MS
Description
physical association
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
InR 

InR

bait

TAP tag

chico

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Overall SAINT probability of interaction = 1.

FBrf0230883-12.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
InR 

InR

bait

TAP tag

chico

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0233454-133.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

chico

bait

TAP tag

InR 

InR

prey

SAINT score = 1

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

Observed in two of three conditions tested: uninduced cells and 10 minutes after insulin stimulation.

FBrf0233454-466.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
InR 

InR

bait

TAP tag

chico

prey

SAINT score = 1

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

Observed in all three conditions tested: uninduced cells, 10 minutes after insulin stimulation and 30 minutes after insulin stimulation.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (7)