deletion of this region decreases binding by 70%
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/B42-AD
The enrichment of candidate chico-interacting proteins in control versus experimental samples was corrected for relative protein abundance in the lysates. Only three chico-interacting candidates were considered to be enriched above the threshold expected for abundant contaminant proteins: 14-3-3ε, 14-3-3ζ and InR.
Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.
SAINT score = 1
Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Overall SAINT probability of interaction = 1.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.
Observed in two of three conditions tested: uninduced cells and 10 minutes after insulin stimulation.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.
Observed in all three conditions tested: uninduced cells, 10 minutes after insulin stimulation and 30 minutes after insulin stimulation.
