Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Axn binds Gαo regardless of its bound nucleotide (GDP vs GTP) or the presence/absence of a myristoyl moiety. Axn does not affect GTP hydrolysis by Gαo. dsh binds pig-derived Gβ/Gγ, but not to Gαo.
