FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pit, Su(var)205
FlyBase ID
FBig0000123949
Interaction Network
Interactions Browser links
pit network
Su(var)205 network
Reported Interactions
FBrf0264195-1.PL.WB
Reference
Rajshekar et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
pit 

pit

bait

APEX2 tag

Su(var)205 

HP1a

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from stably transfected construct; prey produced from endogenous gene.

(Rajshekar et al., 2025)

After addition of biotin, Su(var)205 was biotinylated by pit-APEX2 and pulled down by streptavadin.

(Rajshekar et al., 2025)
FBrf0264195-2.coIP.WB
Reference
Rajshekar et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
pit 

pit

bait
Su(var)205 

HP1a

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear cell extract of S2R+ cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Rajshekar et al., 2025)
FBrf0264195-3.PD.WB
Reference
Rajshekar et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pit 

pit

bait

His tag

pit 
Su(var)205 

HP1a

prey
Su(var)205 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pit 
C-terminal residues 629 and 633
necessary binding region
aa 629 and 633

V629E, V633E; coordinates relative to pit-PA

Su(var)205 
chromo-shadow dimerization domain residue 119
necessary binding region
aa 119

I191E; coordinates relative to Su(var)205-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and produced as recombinant fusion proteins in bacterial system.

(Rajshekar et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (1)