An umbrella record for Lai lab shortRNA-seq coverage profiles consolidated from shortRNA-seq assays of various developmental stages.
Data from short RNA-Seq assays from multiple, independent studies have been analyzed in parallel and consolidated into data for 13 developmental stages. To generate bigWig short RNA transcriptome profiles, reads for these assays were mapped to D. melanogaster Release 6 (FlyBase r6.09). Multiple libraries were consolidated per condition by summing the number of reads per genomic position and normalizing this count by dividing by the total number of reads summed over all libraries for this condition and multiplying by 1,000,000. Basically, this normalization is equivalent to reads per million (RPM), but on a per nucleotide basis. For reads mapping to multiple locations, the coverage values were equally weighted across all mapping sites.