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Citation
Steller, H., Pirrotta, V. (1985). Expression of the Drosophila white gene under the control of the hsp-70 heat shock promoter.  EMBO J. 4(13B): 3765--3772.
FlyBase ID
FBrf0042439
Publication Type
Research paper
Abstract

We have used P-mediated transformation to introduce into the Drosophila genome transposons containing the hsp-70 heat shock promoter (hsp) placed at different distances upstream of the white gene. Using these constructions we found a strong terminator 250 nucleotides upstream of the white transcription start. When all white upstream sequences are deleted and the hsp is fused to the white leader region, white transcription is under hsp control, however, flies carrying this construction have red eyes even at low temperature, due to the basal level of activity of the hsp. High levels of transcription are obtained after heat shock but about half of the transcripts are incompletely spliced. One transformed line carries the transposon integrated near the heterochromatin. These flies have very pale eyes but develop strongly pigmented, variegated eyes if heat shocked in the first 2 days of the pupal stage. Heat shocks after eclosion have no effect on eye pigmentation. The properties of these transposons also indicated that the control sequences required for Zeste interaction lie > 1 kb upstream of the white transcription start while those required for dosage compensation are close to the gene.

PubMed ID
PubMed Central ID
PMC554729 (PMC) (EuropePMC)
DOI
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    EMBO J.
    Title
    The EMBO Journal
    Publication Year
    1982-
    ISBN/ISSN
    0261-4189
    Data From Reference
    Aberrations (1)
    Alleles (3)
    Genes (2)
    Molecular Constructs (2)
    Insertions (1)
    Transgenic Constructs (2)