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Citation
Laski, F.A., Ganguly, S., Sharp, P.A., RajBhandary, U.L., Rubin, G.M. (1989). Construction, stable transformation, and function of an amber suppressor tRNA gene in Drosophila melanogaster.  Proc. Natl. Acad. Sci. U.S.A. 86(): 6696--6698.
FlyBase ID
FBrf0050858
Publication Type
Research paper
Abstract

Drosophila melanogaster strains with a stably incorporated amber suppressor tRNA gene have been generated. A tRNATyr gene was site specifically mutated to produce an anticodon sequence that recognizes the amber codon and then introduced into Drosophila by using P-element-mediated transformation. Transformants from four integration events were recovered. Two integrations resulted in both male and female sterility, whereas the other two resulted in male sterility but female fertility. Strains derived from the two female-fertile integration events were shown to have a low level of amber-suppressing activity by their ability to suppress an amber mutation in a chloramphenicol acetyltransferase gene.

PubMed ID
PubMed Central ID
PMC297912 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Alleles (5)
    Genes (4)
    Transgenic Constructs (4)