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Citation
Heslip, T.R., Williams, J.A., Bell, J.B., Hodgetts, R.B. (1992). A P-element chimera containing captured genomic sequences was recovered at the vestigial locus in Drosophila following targeted transposition.  Genetics 131: 917--927.
FlyBase ID
FBrf0056271
Publication Type
Research paper
Abstract

A P element carrying the Dopa decarboxylase gene, P[Ddc], was targeted into vg21, a cryptic P element induced mutant allele of the vestigial (vg) locus. The resulting allele, vg28w, contained the expected P[Ddc] plus an additional 9.5 kb of DNA, captured from elsewhere on chromosome II. Reversion of the vg28w mutant allele demonstrated that the entire insert can excise but cannot reinsert at an appreciable frequency. We explain the targeted transposition as the repair of a double stranded gap, created by the excision of the P element at vg21, and suggest that the formation of chimeric elements may be an important component of P element dependent genomic instability.

PubMed ID
PubMed Central ID
PMC1205102 (PMC) (EuropePMC)
DOI
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference