Open Close
Kalb, J.M., DiBenedetto, A.J., Wolfner, M.F. (1993). Probing the function of Drosophila melanogaster accessory glands by directed cell ablation.  Proc. Natl. Acad. Sci. U.S.A. 90(17): 8093--8097.
FlyBase ID
Publication Type
Research paper

The female Drosophila melanogaster fly undergoes behavioral changes after mating, including an increase in egg laying and an avoidance of remating. Accessory-gland products elicit these changes transiently when introduced into unmated female flies. We report here the generation and phenotype of flies that lack functional accessory-gland main cells as a consequence of genetically directed delivery of diphtheria toxin subunit A to those cells. Only main-cell secretions are essential for the short-term inhibition to remating; no other products of the genital tract can replace their function. Long-term inhibition to remating depends only on the storage of sperm in the female. Both sperm and main-cell secretions have roles in the increase of egg laying by the mated female. In addition to full-strength diphtheria toxin, we used low-activity toxins to kill only those cells that express toxin at high levels. These transgenic strains that express diphtheria toxins of different strengths in accessory-gland main cells will be useful in further defining the role of these cells.

PubMed ID
PubMed Central ID
PMC47294 (PMC) (EuropePMC)
Associated Information
Associated Files
Other Information
Secondary IDs
    Language of Publication
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Proc. Natl. Acad. Sci. U.S.A.
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    Data From Reference
    Alleles (7)
    Genes (8)
    Experimental Tools (3)
    Transgenic Constructs (4)