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Citation
Krishnan, S.N., Frei, E., Schalet, A.P., Wyman, R.J. (1995). Molecular basis of intracistronic complementation in the Passover locus of Drosophila.  Proc. Natl. Acad. Sci. U.S.A. 92(6): 2021--2025.
FlyBase ID
FBrf0080175
Publication Type
Research paper
Abstract

The only demonstrated mechanism for intracistronic genetic complementation requires physical interaction of protein subunits to create a functional molecule. We demonstrate another and perhaps quite general mechanism utilizing proteins with unique and shared domains. The Drosophila neural mutant Passover (Pas) disrupts specific synaptic connections. Alleles of a lethal complementation group exhibit a complex pattern of complementation with Pas alleles. Whereas all heterozygotes between these lethal alleles and Pas are viable, only some alleles complement the neural defect of Pas. Lethal and neural functions are separately encoded by two proteins that have distinct N-terminal domains and a common C-terminal portion. Neural-specific and lethal-specific mutations map to unique exons, while neural-lethal mutations map to shared exons. Combinations of lethal and neural alleles result in production of both proteins and demonstrate intracistronic complementation.

PubMed ID
PubMed Central ID
PMC42415 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Aberrations (3)
    Alleles (14)
    Genes (2)
    Insertions (1)