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Bomer, U., Rassow, J., Zufall, N., Pfanner, N., Meijer, M., Maarse, A.C. (1996). The preprotein translocase of the inner mitochondrial membrane: evolutionary conservation of targeting and assembly of Tim17.  J. Mol. Biol. 262(4): 389--395.
FlyBase ID
FBrf0090402
Publication Type
Research paper
Abstract

The preprotein translocase of the inner mitochondrial membrane has only been described in Saccharomyces cerevisiae to date. We report that the essential subunit Tim17 is highly conserved in evolution. The targeting and assembly of yeast Tim17 as well as that of human and Drosophila melanogaster Tim17 were characterized with isolated yeast mitochondria. Targeting signals in the mature protein direct the Tim17 precursors to the receptor Tom70 on the mitochondrial surface. In a membrane potential-dependent step the precursors insert into the inner membrane, adopt a characteristic topology and assemble with Tim23. The mechanisms of targeting and assembly were indistinguishable between the Tim17s from distinct organisms, indicating a high evolutionary conservation.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Mol. Biol.
    Title
    Journal of Molecular Biology
    Publication Year
    1959-
    ISBN/ISSN
    0022-2836
    Data From Reference
    Genes (1)