Open Close
Reference
Citation
Henchoz, S., De Rubertis, F., Pauli, D., Spierer, P. (1996). The dose of a putative ubiquitin-specific protease affects position-effect variegation in Drosophila melanogaster.  Mol. Cell. Biol. 16(10): 5717--5725.
FlyBase ID
FBrf0090605
Publication Type
Research paper
Abstract

A dominant insertional P-element mutation enhances position-effect variegation in Drosophila melanogaster. The mutation is homozygous, viable, and fertile and maps at 64E on the third chromosome. The corresponding gene was cloned by transposon tagging. Insertion of the transposon upstream of the open reading frame correlates with a strong reduction of transcript level. A transgene was constructed with the cDNA and found to have the effect opposite from that of the mutation, namely, to suppress variegation. Sequencing of the cDNA reveals a large open reading frame encoding a putative ubiquitin-specific protease (Ubp). Ubiquitin marks various proteins, frequently for proteasome-dependent degradation. Ubps can cleave the ubiquitin part from these proteins. We discuss the link established here between a deubiquitinating enzyme and epigenetic silencing processes.

PubMed ID
PubMed Central ID
PMC231572 (PMC) (EuropePMC)
DOI
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Cell. Biol.
    Title
    Molecular and Cellular Biology
    Publication Year
    1981-
    ISBN/ISSN
    0270-7306
    Data From Reference
    Aberrations (4)
    Alleles (8)
    Genes (8)
    Insertions (1)
    Transgenic Constructs (1)