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Taillebourg, E., Dura, J.M. (1999). A novel mechanism for P element homing in Drosophila.  Proc. Natl. Acad. Sci. U.S.A. 96(12): 6856--6861.
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Research paper

P element insertion is essentially random at the scale of the genome. However, P elements containing regulatory sequences from Drosophila engrailed and polyhomeotic genes and from the Bithorax and Antennapedia complexes show some insertional specificity by frequently inserting near the parent gene (homing) and/or near genes containing Polycomb group response elements (preferential insertion). This phenomenon is thought to be mediated by Polycomb group proteins. In this report, we describe a case of homing of P elements containing regulatory sequences of the linotte gene. This homing occurs with high frequency (up to 20% of the lines) and high precision (inserted into a region of <1 kilobase). We present evidence showing that it is not mediated by Polycomb group proteins but by a new, as yet unknown, mechanism. We also suggest that P element homing could be a more frequent phenomenon than generally assumed and that it could become a powerful tool of Drosophila reverse genetics, for which there is no other described gene targeting technique.

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PubMed Central ID
PMC22006 (PMC) (EuropePMC)
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    Proc. Natl. Acad. Sci. U.S.A.
    Proceedings of the National Academy of Sciences of the United States of America
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    Aberrations (1)
    Alleles (21)
    Genes (16)
    Insertions (3)
    Experimental Tools (1)
    Transgenic Constructs (4)