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Newsome, T.P., Schmidt, S., Dietzl, G., Keleman, K., Asling, B., Debant, A., Dickson, B.J. (2000). Trio combines with Dock to regulate Pak activity during photoreceptor axon pathfinding in Drosophila.  Cell 101(3): 283--294.
FlyBase ID
FBrf0128589
Publication Type
Research paper
Abstract

Correct pathfinding by Drosophila photoreceptor axons requires recruitment of p21-activated kinase (Pak) to the membrane by the SH2-SH3 adaptor Dock. Here, we identify the guanine nucleotide exchange factor (GEF) Trio as another essential component in photoreceptor axon guidance. Regulated exchange activity of one of the two Trio GEF domains is critical for accurate pathfinding. This GEF domain activates Rac, which in turn activates Pak. Mutations in trio result in projection defects similar to those observed in both Pak and dock mutants, and trio interacts genetically with Rac, Pak, and dock. These data define a signaling pathway from Trio to Rac to Pak that links guidance receptors to the growth cone cytoskeleton. We propose that distinct signals transduced via Trio and Dock act combinatorially to activate Pak in spatially restricted domains within the growth cone, thereby controlling the direction of axon extension.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Aberrations (2)
    Alleles (52)
    Genes (18)
    Physical Interactions (6)
    Insertions (3)
    Experimental Tools (6)
    Transgenic Constructs (29)
    Transcripts (2)