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Citation
Schwartz, C.J.E., Sampson, H.M., Hlousek, D., Percival-Smith, A., Copeland, J.W.R., Simmonds, A.J., Krause, H.M. (2001). FTZ-Factor1 and Fushi tarazu interact via conserved nuclear receptor and coactivator motifs.  EMBO J. 20(3): 510--519.
FlyBase ID
FBrf0134590
Publication Type
Research paper
Abstract

To activate transcription, most nuclear receptor proteins require coactivators that bind to their ligand-binding domains (LBDs). The Drosophila FTZ-Factor1 (FTZ-F1) protein is a conserved member of the nuclear receptor superfamily, but was previously thought to lack an AF2 motif, a motif that is required for ligand and coactivator binding. Here we show that FTZ-F1 does have an AF2 motif and that it is required to bind a coactivator, the homeodomain-containing protein Fushi tarazu (FTZ). We also show that FTZ contains an AF2-interacting nuclear receptor box, the first to be found in a homeodomain protein. Both interaction motifs are shown to be necessary for physical interactions in vitro and for functional interactions in developing embryos. These unexpected findings have important implications for the conserved homologs of the two proteins.

PubMed ID
PubMed Central ID
PMC133472 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    EMBO J.
    Title
    The EMBO Journal
    Publication Year
    1982-
    ISBN/ISSN
    0261-4189
    Data From Reference
    Alleles (9)
    Genes (9)
    Physical Interactions (3)
    Natural transposons (1)
    Experimental Tools (1)
    Transgenic Constructs (7)